Rw3G029180

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003309
Physical Location & Seq
Forward (+)
44641547 .. 44645741
4195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G029180.1

Sequence Viewer

Length: 594 bp
ATGGACTCCGAATCAGTGATTGAGTTTCTGGGCTGCGTTCCTCTGCTTCAATGCCTACCCAGCTCCTCCCTAAAGAAAATCGCCCAACTCGTAATTCCAAAGCACTACGATAAAGGAGAGTATGTTGTTCATGAAGTTGTCAAACTGCTGGGGAGTGGACAATATATCCGTGGATCCACAGCAATTGTAATTCGCTTTCAGATGATTCGTCCCGTTTCAATACCAGATATTCATCCCTCTAGTGCAGGGATTACTGAAGGCTTTTCTATGTGTGGCGGTGACTTTGTTGAGGTTTATAACGATCCAAGCCAAGTAGGTAATTTTATCAAGTTTCATAGGTTTCTACCATTGCAATATTGGAATTTAAATTACATTCTAGTAGATACATTTGTGCCTCCTAAAAAGGAAAGTTCTTTTGAAGTGATCAGGGATATTGTTTGTTGTGGAGTTTGCGATTTCAGTAATTGGTTGGAAGAGGTCGGAGGTCAGATTTCAGTAATTGTTGTGGGTCTTCATTCGCCAGATAAACCATTGTCACATAGAGTTCAAGTAGTACAGCAAGCAAGGTTAAGCTTCAATGGAGAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

197

Amino Acids

21.91

Weight (kDa)

5.84

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CARME PF07942 67 - 103 4.4e-06 Carnosine N-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
AciI CCGC 1 cut(s) 276
AclWI GGATC 3 cut(s) 168, 181, 296
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 1 cut(s) 555
AgsI TTSAA 5 cut(s) 50, 219, 419, 548, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 3 cut(s) 63, 573, 587
AluI AGCT 3 cut(s) 63, 573, 587
AlwI GGATC 3 cut(s) 168, 181, 296
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 290
BaeI ACNNNNGTAYC 2 cut(s) 375, 408
BamHI GGATCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 20
BclI TGATCA 1 cut(s) 423
BfaI CTAG 2 cut(s) 240, 377
BisI GCNGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 35
BmiI GGNNCC 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bse3DI GCAATG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 1 cut(s) 231
BseMI GCAATG 1 cut(s) 347
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 20
BseYI CCCAGC 2 cut(s) 59, 148
BsgI GTGCAG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 195
Bsp143I GATC 3 cut(s) 173, 301, 423
BspACI CCGC 1 cut(s) 276
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 175
BspPI GGATC 3 cut(s) 168, 181, 296
BsrDI GCAATG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 3 cut(s) 173, 301, 423
Bst6I CTCTTC 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 561
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 232
BstKTI GATC 3 cut(s) 176, 304, 426
BstMBI GATC 3 cut(s) 173, 301, 423
BstMWI GCNNNNNNNGC 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 20
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 561
CciI TCATGA 1 cut(s) 130
Csp6I GTAC 1 cut(s) 554
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 1 cut(s) 131
CviJI RGCY 6 cut(s) 33, 63, 261, 309, 573, 587
CviKI_1 RGCY 6 cut(s) 33, 63, 261, 309, 573, 587
CviQI GTAC 1 cut(s) 554
DpnI GATC 3 cut(s) 175, 303, 425
DpnII GATC 3 cut(s) 173, 301, 423
DraI TTTAAA 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 276
FaeI CATG 1 cut(s) 134
FaiI YATR 7 cut(s) 123, 132, 165, 269, 297, 336, 540
FaqI GGGAC 1 cut(s) 195
FatI CATG 1 cut(s) 130
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 1 cut(s) 34
FokI GGATG 1 cut(s) 219
Fsp4HI GCNGC 1 cut(s) 34
FspBI CTAG 2 cut(s) 240, 377
GluI GCNGC 1 cut(s) 34
GsaI CCCAGC 2 cut(s) 63, 152
Hin1II CATG 1 cut(s) 134
HindIII AAGCTT 2 cut(s) 571, 585
HinfI GANTC 3 cut(s) 5, 11, 205
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 4 cut(s) 10, 201, 482, 489
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 1 cut(s) 251
HpyCH4V TGCA 2 cut(s) 245, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 60
Hsp92II CATG 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 3 cut(s) 173, 301, 423
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 7 cut(s) 14, 73, 134, 231, 237, 412, 534
Lsp1109I GCAGC 1 cut(s) 20
MaeI CTAG 2 cut(s) 240, 377
MaeIII GTNAC 2 cut(s) 278, 534
MalI GATC 3 cut(s) 175, 303, 425
MboI GATC 3 cut(s) 173, 301, 423
MboII GAAGA 2 cut(s) 485, 503
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 173
MluCI AATT 8 cut(s) 93, 183, 189, 319, 361, 367, 463, 498
MmeI TCCRAC 2 cut(s) 450, 460
MnlI CCTC 7 cut(s) 51, 76, 247, 283, 405, 469, 476
MseI TTAA 3 cut(s) 365, 569, 592
MunI CAATTG 1 cut(s) 183
MwoI GCNNNNNNNGC 1 cut(s) 60
NdeII GATC 3 cut(s) 173, 301, 423
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 278, 534
PagI TCATGA 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 2 cut(s) 11, 205
PkrI GCNGC 1 cut(s) 35
PsiI TTATAA 1 cut(s) 297
PspFI CCCAGC 2 cut(s) 59, 148
PspN4I GGNNCC 1 cut(s) 175
PsuI RGATCY 1 cut(s) 173
RsaI GTAC 1 cut(s) 555
RsaNI GTAC 1 cut(s) 554
SaqAI TTAA 3 cut(s) 365, 569, 592
SatI GCNGC 1 cut(s) 34
Sau3AI GATC 3 cut(s) 173, 301, 423
SetI ASST 9 cut(s) 65, 294, 319, 341, 480, 487, 569, 575, 589
SmiI ATTTAAAT 1 cut(s) 366
Sse9I AATT 8 cut(s) 93, 183, 189, 319, 361, 367, 463, 498
SsiI CCGC 1 cut(s) 276
SspI AATATT 1 cut(s) 356
SspMI CTAG 2 cut(s) 240, 377
SwaI ATTTAAAT 1 cut(s) 366
TasI AATT 8 cut(s) 93, 183, 189, 319, 361, 367, 463, 498
TatI WGTACW 1 cut(s) 553
TfiI GAWTC 2 cut(s) 11, 205
Tru1I TTAA 3 cut(s) 365, 569, 592
Tru9I TTAA 3 cut(s) 365, 569, 592
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 2 cut(s) 278, 534
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 2 cut(s) 278, 534
TspDTI ATGAA 5 cut(s) 119, 147, 221, 323, 503
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 1 cut(s) 21
XapI RAATTY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XspI CTAG 2 cut(s) 240, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.