AT4G00520

acyl-CoA thioesterase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003309
Physical Location & Seq
Forward (+)
227650 .. 231751
4102 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00520.3

Sequence Viewer

Length: 1323 bp
ATGATTGAATTTGCCGGCCAACGTTTCGACAGTCTCTACACCGTCATGAACACTGAATCAGTTGTCGAGTTCCTTGGGAATGTGACCTTGTTGCAGCGGTTACCTAGTTCCTCTCTGAAGAGAATCTCCGAAGTCGTTGTCTTCAAAGGTTATGACAGAGGTGATTATGTGGTTCGTGAAAATCAAAATGTGGATGGAGTTTATTTTCTCTTGCAAGGACAGGCTCAGGTTCTGAGATCAGCCGGAGAGGAAAACTATCAAGAGTTCCCTTTGAAACGATATGATTTCTTCGGCCATGGTATTTTCGGGGATGTTTACTCAGCAGATGTTGTTGCTGTGACAGAGCTTACCTGCTTGCTGTTGATGTCTGATCATCGTGCTTTACTTGAAATAAAGTCAGTCTCGGATTCAGATAAGGAACGCTGTCTTGTGGAAGACATACTATATCTAGAACCATTAGATTTGAATGTATACCGGGGGTTCACCCCACCTAATGCTCCAACCTATGGAAAGGTTTATGGAGGGCAATTAGTTGGACAGGCACTTGCCGCAGCATCAAAAACTGTTGAAACTATGAAGATAGTCCATAATTTTCATTGCTATTTCCTTCTTGTTGGAGATATAAATATTCCCATCATATATGATGTTAACCGCTTACGTGACGGCAACAACTTTGCCACCAGAAGTGTAGATGCTAGACAGAAAGGAAAAACTATATTCACCTTGTTCGCGTCATTTCAGAAAAAGCAACAAGGTTTTATTCACCAGGAGTCGACCATGCCTCATACACCAGCTCCTGAAACGCTTCTACCAAGGGAGGAGATGCTTGAACGGCTTGTTACTGAGCCTCTGCTACCTAGGGATTACCGAAACCAAGTTGCAACTGAAATTAGTGTTCCATTCCCTATAGATATTCGATTTTGTGAGCCAAATCGTTCCACTAAACAGAATAAGTCTCCTCCAAGACTAAAATATTGGTTTAGAGCAAAGGGAAAACTTTCTGATGATGATCAAGCTTTGCACAGATGTGTGGTTGCATTTGCTTCCGATTTGATATTCGCCACTATCAGTTTAAACCCTCACCGGAGAGAGGGCATGAGTGTAGCTGCTCTTAGCCTGGACCACTCGATGTGGTTCCACCGACCTGTAAGAGCAGATGATTGGTTGTTGTTTGTGATAGTGAGTCCAACTGCGACCGAAAGCCGCGGTTTTGCAACTGGCAAAATGTTCAACAGAAAGGGAGAGCTGGTGGTATCATTGACGCAAGAAGCTGTGTTAAGAGAAGCTGTGACTATTAAGCCATCCTTCGGGGCCAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

440

Amino Acids

49.85

Weight (kDa)

6.81

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 6 - 74 1.9e-09 Cyclic nucleotide-binding domain
4HBT_3 PF13622 116 - 200 2.8e-17 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 267 - 378 5e-25 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 272 - 376 4.7e-23 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 506
AccI GTMKAC 2 cut(s) 471, 773
AccII CGCG 2 cut(s) 731, 1206
AciI CCGC 5 cut(s) 97, 549, 652, 1204, 1206
AclI AACGTT 1 cut(s) 22
AcoI YGGCCR 2 cut(s) 16, 292
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 137
AfiI CCNNNNNNNGG 3 cut(s) 506, 1090, 1307
AgsI TTSAA 8 cut(s) 8, 145, 274, 389, 466, 569, 830, 1231
AjnI CCWGG 2 cut(s) 765, 1116
AleI CACNNNNGTG 1 cut(s) 1026
AluBI AGCT 8 cut(s) 346, 794, 1016, 1106, 1246, 1271, 1286, 1318
AluI AGCT 8 cut(s) 346, 794, 1016, 1106, 1246, 1271, 1286, 1318
Alw26I GTCTC 3 cut(s) 38, 406, 960
AlwNI CAGNNNCTG 2 cut(s) 232, 797
AoxI GGCC 3 cut(s) 16, 292, 1311
ApeKI GCWGC 3 cut(s) 94, 551, 1106
ApoI RAATTY 1 cut(s) 8
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
Asp700I GAANNNNTTC 2 cut(s) 804, 997
AspA2I CCTAGG 1 cut(s) 857
AspS9I GGNCC 2 cut(s) 1120, 1311
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 5 cut(s) 173, 475, 712, 755, 1073
AvaII GGWCC 1 cut(s) 1120
AvrII CCTAGG 1 cut(s) 857
BbsI GAAGAC 2 cut(s) 133, 441
BbvI GCAGC 3 cut(s) 106, 563, 1093
BccI CCATC 3 cut(s) 188, 641, 1309
BceAI ACGGC 2 cut(s) 679, 848
BciT130I CCWGG 2 cut(s) 767, 1118
BclI TGATCA 2 cut(s) 370, 1009
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 3 cut(s) 38, 406, 960
BfaI CTAG 4 cut(s) 105, 449, 696, 858
BfmI CTRYAG 1 cut(s) 906
BfuAI ACCTGC 1 cut(s) 359
BisI GCNGC 5 cut(s) 95, 549, 552, 1107, 1204
BlnI CCTAGG 1 cut(s) 857
BlsI GCNGC 5 cut(s) 96, 550, 553, 1108, 1205
Bme1390I CCNGG 3 cut(s) 476, 767, 1118
Bme18I GGWCC 1 cut(s) 1120
BmgT120I GGNCC 2 cut(s) 1120, 1311
BmiI GGNNCC 2 cut(s) 1136, 1312
BmrFI CCNGG 3 cut(s) 476, 767, 1118
BmsI GCATC 3 cut(s) 563, 682, 813
BpiI GAAGAC 2 cut(s) 133, 441
Bpu10I CCTNAGC 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 1008
BsaJI CCNNGG 6 cut(s) 73, 295, 475, 812, 857, 1204
BsaWI WCCGGW 1 cut(s) 1083
BsaXI ACNNNNNCTCC 4 cut(s) 778, 808, 1233, 1263
Bsc4I CCNNNNNNNGG 3 cut(s) 506, 1090, 1307
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 1222
Bse3DI GCAATG 1 cut(s) 595
Bse8I GATNNNNATC 1 cut(s) 1008
BseBI CCWGG 2 cut(s) 767, 1118
BseDI CCNNGG 6 cut(s) 73, 295, 475, 812, 857, 1204
BseGI GGATG 3 cut(s) 199, 316, 1301
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 3 cut(s) 506, 1090, 1307
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 4 cut(s) 224, 239, 333, 834
BseNI ACTGG 1 cut(s) 1222
BseRI GAGGAG 2 cut(s) 833, 948
BseXI GCAGC 3 cut(s) 106, 563, 1093
Bsh1236I CGCG 2 cut(s) 731, 1206
Bsh1285I CGRYCG 1 cut(s) 1197
BshFI GGCC 3 cut(s) 18, 294, 1313
BsiEI CGRYCG 1 cut(s) 1197
BsiSI CCGG 4 cut(s) 15, 243, 475, 1084
BslI CCNNNNNNNGG 3 cut(s) 506, 1090, 1307
BsmAI GTCTC 3 cut(s) 38, 406, 960
BsnI GGCC 3 cut(s) 18, 294, 1313
Bsp143I GATC 3 cut(s) 236, 370, 1009
Bsp19I CCATGG 1 cut(s) 295
BspACI CCGC 5 cut(s) 97, 549, 652, 1204, 1206
BspANI GGCC 3 cut(s) 18, 294, 1313
BspCNI CTCAG 4 cut(s) 225, 238, 332, 835
BspFNI CGCG 2 cut(s) 731, 1206
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 2 cut(s) 1136, 1312
BspMI ACCTGC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 1 cut(s) 1222
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 6 cut(s) 73, 295, 475, 812, 857, 1204
BssMI GATC 3 cut(s) 236, 370, 1009
BssNAI GTATAC 1 cut(s) 472
BssT1I CCWWGG 4 cut(s) 73, 295, 812, 857
Bst1107I GTATAC 1 cut(s) 472
Bst2UI CCWGG 2 cut(s) 767, 1118
Bst4CI ACNGT 3 cut(s) 32, 43, 565
Bst6I CTCTTC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 16, 356
BstDEI CTNAG 5 cut(s) 225, 233, 319, 843, 1112
BstDSI CCRYGG 2 cut(s) 295, 1204
BstEII GGTNACC 1 cut(s) 99
BstF5I GGATG 3 cut(s) 199, 316, 1301
BstFNI CGCG 2 cut(s) 731, 1206
BstKTI GATC 3 cut(s) 239, 373, 1012
BstMAI GTCTC 3 cut(s) 38, 406, 960
BstMBI GATC 3 cut(s) 236, 370, 1009
BstMCI CGRYCG 1 cut(s) 1197
BstMWI GCNNNNNNNGC 2 cut(s) 548, 832
BstNI CCWGG 2 cut(s) 767, 1118
BstPI GGTNACC 1 cut(s) 99
BstSCI CCNGG 3 cut(s) 474, 765, 1116
BstSFI CTRYAG 1 cut(s) 906
BstUI CGCG 2 cut(s) 731, 1206
BstV1I GCAGC 3 cut(s) 106, 563, 1093
BstV2I GAAGAC 2 cut(s) 133, 441
BstZ17I GTATAC 1 cut(s) 472
BsuRI GGCC 3 cut(s) 18, 294, 1313
BtgI CCRYGG 2 cut(s) 295, 1204
BtsCI GGATG 3 cut(s) 199, 316, 1301
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 2 cut(s) 16, 356
CaiI CAGNNNCTG 2 cut(s) 232, 797
CciI TCATGA 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 1120, 1311
Cfr42I CCGCGG 1 cut(s) 1207
CseI GACGC 2 cut(s) 720, 1270
CviAII CATG 4 cut(s) 46, 296, 778, 1096
DdeI CTNAG 5 cut(s) 225, 233, 319, 843, 1112
DpnI GATC 3 cut(s) 238, 372, 1011
DpnII GATC 3 cut(s) 236, 370, 1009
DraI TTTAAA 1 cut(s) 1074
EaeI YGGCCR 2 cut(s) 16, 292
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 4 cut(s) 73, 295, 812, 857
Eco47I GGWCC 1 cut(s) 1120
Eco57I CTGAAG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 765, 1116
EcoT14I CCWWGG 4 cut(s) 73, 295, 812, 857
ErhI CCWWGG 4 cut(s) 73, 295, 812, 857
FaeI CATG 4 cut(s) 49, 299, 781, 1099
FalI AAGNNNNNCTT 2 cut(s) 1289, 1321
FatI CATG 4 cut(s) 45, 295, 777, 1095
FbaI TGATCA 2 cut(s) 370, 1009
FblI GTMKAC 2 cut(s) 471, 773
Fnu4HI GCNGC 5 cut(s) 95, 549, 552, 1107, 1204
FokI GGATG 3 cut(s) 206, 323, 1288
Fsp4HI GCNGC 5 cut(s) 95, 549, 552, 1107, 1204
FspBI CTAG 4 cut(s) 105, 449, 696, 858
GluI GCNGC 5 cut(s) 95, 549, 552, 1107, 1204
HaeIII GGCC 3 cut(s) 18, 294, 1313
HapII CCGG 4 cut(s) 15, 243, 475, 1084
HgaI GACGC 2 cut(s) 720, 1270
Hin1II CATG 4 cut(s) 49, 299, 781, 1099
HincII GTYRAC 2 cut(s) 649, 774
HindII GTYRAC 2 cut(s) 649, 774
HindIII AAGCTT 1 cut(s) 1014
HinfI GANTC 5 cut(s) 56, 123, 407, 770, 1183
HpaI GTTAAC 1 cut(s) 649
HpaII CCGG 4 cut(s) 15, 243, 475, 1084
HphI GGTGA 5 cut(s) 173, 475, 712, 755, 1073
Hpy166II GTNNAC 5 cut(s) 316, 472, 483, 649, 774
Hpy188I TCNGA 9 cut(s) 117, 130, 234, 370, 406, 412, 741, 1003, 1048
Hpy188III TCNNGA 5 cut(s) 46, 176, 260, 449, 797
Hpy8I GTNNAC 5 cut(s) 316, 472, 483, 649, 774
HpyAV CCTTC 2 cut(s) 617, 1315
HpyCH4III ACNGT 3 cut(s) 32, 43, 565
HpyCH4IV ACGT 2 cut(s) 22, 658
HpyCH4V TGCA 6 cut(s) 94, 214, 881, 1021, 1037, 1214
HpyF10VI GCNNNNNNNGC 2 cut(s) 548, 832
HpyF3I CTNAG 5 cut(s) 225, 233, 319, 843, 1112
HpySE526I ACGT 2 cut(s) 22, 658
Hsp92II CATG 4 cut(s) 49, 299, 781, 1099
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Ksp22I TGATCA 2 cut(s) 370, 1009
KspAI GTTAAC 1 cut(s) 649
KspI CCGCGG 1 cut(s) 1207
Kzo9I GATC 3 cut(s) 236, 370, 1009
LmnI GCTCC 2 cut(s) 502, 799
Lsp1109I GCAGC 3 cut(s) 106, 563, 1093
LweI GCATC 3 cut(s) 563, 682, 813
MaeI CTAG 4 cut(s) 105, 449, 696, 858
MaeII ACGT 2 cut(s) 22, 658
MaeIII GTNAC 6 cut(s) 82, 99, 337, 659, 838, 1288
MalI GATC 3 cut(s) 238, 372, 1011
MboI GATC 3 cut(s) 236, 370, 1009
MboII GAAGA 5 cut(s) 130, 133, 280, 446, 589
MluCI AATT 4 cut(s) 8, 527, 589, 888
MlyI GAGTC 2 cut(s) 779, 1192
MmeI TCCRAC 4 cut(s) 514, 524, 595, 1211
MroNI GCCGGC 1 cut(s) 14
MroXI GAANNNNTTC 2 cut(s) 804, 997
MseI TTAA 4 cut(s) 648, 1073, 1277, 1296
MslI CAYNNNNRTG 2 cut(s) 44, 1026
MspA1I CMGCKG 2 cut(s) 97, 1206
MspI CCGG 4 cut(s) 15, 243, 475, 1084
MspR9I CCNGG 3 cut(s) 476, 767, 1118
MssI GTTTAAAC 1 cut(s) 1074
MvaI CCWGG 2 cut(s) 767, 1118
MvnI CGCG 2 cut(s) 731, 1206
MwoI GCNNNNNNNGC 2 cut(s) 548, 832
NaeI GCCGGC 1 cut(s) 16
NciI CCSGG 1 cut(s) 476
NcoI CCATGG 1 cut(s) 295
NdeII GATC 3 cut(s) 236, 370, 1009
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 4 cut(s) 49, 299, 781, 1099
NlaIV GGNNCC 2 cut(s) 1136, 1312
NmuCI GTSAC 4 cut(s) 82, 337, 659, 1288
OliI CACNNNNGTG 1 cut(s) 1026
PagI TCATGA 1 cut(s) 45
PdiI GCCGGC 1 cut(s) 16
PdmI GAANNNNTTC 2 cut(s) 804, 997
PfeI GAWTC 3 cut(s) 56, 123, 407
PflMI CCANNNNNTGG 1 cut(s) 506
PkrI GCNGC 5 cut(s) 96, 550, 553, 1108, 1205
PleI GAGTC 2 cut(s) 778, 1191
PmeI GTTTAAAC 1 cut(s) 1074
PpsI GAGTC 2 cut(s) 778, 1191
Ppu21I YACGTR 1 cut(s) 659
Psp1406I AACGTT 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 765, 1116
PspEI GGTNACC 1 cut(s) 99
PspGI CCWGG 2 cut(s) 765, 1116
PspN4I GGNNCC 2 cut(s) 1136, 1312
PspPI GGNCC 2 cut(s) 1120, 1311
PstNI CAGNNNCTG 2 cut(s) 232, 797
RseI CAYNNNNRTG 2 cut(s) 44, 1026
SacII CCGCGG 1 cut(s) 1207
SalI GTCGAC 1 cut(s) 772
SaqAI TTAA 4 cut(s) 648, 1073, 1277, 1296
SatI GCNGC 5 cut(s) 95, 549, 552, 1107, 1204
Sau3AI GATC 3 cut(s) 236, 370, 1009
Sau96I GGNCC 2 cut(s) 1120, 1311
SchI GAGTC 2 cut(s) 779, 1192
ScrFI CCNGG 3 cut(s) 476, 767, 1118
SfaNI GCATC 3 cut(s) 563, 682, 813
SfcI CTRYAG 1 cut(s) 906
Sfr303I CCGCGG 1 cut(s) 1207
SgrBI CCGCGG 1 cut(s) 1207
SinI GGWCC 1 cut(s) 1120
SmiMI CAYNNNNRTG 2 cut(s) 44, 1026
Sse9I AATT 4 cut(s) 8, 527, 589, 888
SsiI CCGC 5 cut(s) 97, 549, 652, 1204, 1206
SspI AATATT 2 cut(s) 628, 974
SspMI CTAG 4 cut(s) 105, 449, 696, 858
StyD4I CCNGG 3 cut(s) 474, 765, 1116
StyI CCWWGG 4 cut(s) 73, 295, 812, 857
TaaI ACNGT 3 cut(s) 32, 43, 565
TaiI ACGT 2 cut(s) 25, 661
TaqI TCGA 5 cut(s) 27, 66, 773, 916, 1127
TaqII GACCGA 1 cut(s) 1211
TasI AATT 4 cut(s) 8, 527, 589, 888
TauI GCSGC 2 cut(s) 551, 1206
TfiI GAWTC 3 cut(s) 56, 123, 407
Tru1I TTAA 4 cut(s) 648, 1073, 1277, 1296
Tru9I TTAA 4 cut(s) 648, 1073, 1277, 1296
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 4 cut(s) 82, 337, 659, 1288
TseI GCWGC 3 cut(s) 94, 551, 1106
Tsp45I GTSAC 4 cut(s) 82, 337, 659, 1288
TspDTI ATGAA 3 cut(s) 62, 584, 590
TspRI CASTG 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 1120
XapI RAATTY 1 cut(s) 8
XbaI TCTAGA 1 cut(s) 448
XmaJI CCTAGG 1 cut(s) 857
XmiI GTMKAC 2 cut(s) 471, 773
XmnI GAANNNNTTC 2 cut(s) 804, 997
XspI CTAG 4 cut(s) 105, 449, 696, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.