MD01G1058300.v1.1

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003309
Physical Location & Seq
Reverse (-)
16264702 .. 16268301
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1058300.v1.1.491

Sequence Viewer

Length: 900 bp
ATGGCCACACATAAAGAATTGTTTTGGTTGATAAGATTATATTCCTTTCCCCCTCCTCTGAAGGTAAACATATTTCAAGGGATTACTTTGCCAGATGCACCAAGTTTCGGACAGGTTTTTGGAGGACAGTTAGTTGGACAGGCATTGGCTGCGGCAACGAAAACTGTTGATTGTCTTAAACTTCTCCATAGTGTGCATGCCCATTTTCTGCTTGCTGGGGATTGTAACACACCAATTATTTATCAAGTTCACAGAGTACGTGATGGGAAGAGCTTTGCCACTCGAAGAGTGGACGCGATACAAAAGGGGATTAACATATTCACATTACTGGCATCATTTAAAAAAGAAGAAGAAGGGTTTGTCCACCAGCAAGCAACAATGCCATCTGTGCCTACTCCGGATAAACTTCTGTCGATGGAAGAACTACGTGAAAAACGTCTGCTTGATCCACGTCTTCTAAGGACTTATCGATTCAAGGCAGCCGCAAAGAAATTTGTGCCTTGGCCTATAGAGATACGATCTTGTGAGCCTAACAGCTCTACCAATCAGACTAAATCTGCACCAAGTGTGAAGTTCTGGTTTAGAGCGATTGGAAAACTTTCAGACGATCAGGCTTTGCATAGATGTGTTGTAGCATATGCTTCGGATTTGATATTTTTGGGTGTAAGTCTGAATCCCCACCGCTTAAGAGTTAGTTTGGACCATGCGATTTGGTTTCACCGTCCGGTTAGAGCTGATGAGTGGTTACTGTATGTGATAGTGACTCCAATTGCCTACGACACACGTGAATTTGTTTCGGGCCAAATGTTTAACATAAAGGGAGAGCTTATTGTATCATTGACTCAAGAGTCCCTAAATAGGCCAATTAGACCAACGCCGGCTGCCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

300

Amino Acids

33.83

Weight (kDa)

9.67

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 33 - 113 7.5e-19 Acyl-CoA thioesterase N-terminal domain
Acyl_CoA_thio PF02551 166 - 283 2.1e-24 Acyl-CoA thioesterase
4HBT_3C PF20789 179 - 284 9.4e-21 Acyl-CoA thioesterase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 847
AccII CGCG 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 3 cut(s) 152, 483, 682
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 491, 788
AcuI CTGAAG 1 cut(s) 80
AcvI CACGTG 1 cut(s) 785
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 107, 858
AflII CTTAAG 1 cut(s) 685
AflIII ACRYGT 1 cut(s) 782
AgsI TTSAA 2 cut(s) 77, 475
AjiI CACGTC 1 cut(s) 452
AluBI AGCT 4 cut(s) 273, 537, 734, 826
AluI AGCT 4 cut(s) 273, 537, 734, 826
AlwI GGATC 1 cut(s) 440
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 4 cut(s) 3, 503, 799, 860
ApeKI GCWGC 3 cut(s) 149, 479, 881
ApoI RAATTY 2 cut(s) 491, 788
Asp700I GAANNNNTTC 1 cut(s) 598
AspS9I GGNCC 2 cut(s) 700, 799
AsuHPI GGTGA 1 cut(s) 710
AvaII GGWCC 1 cut(s) 700
BalI TGGCCA 1 cut(s) 5
BbrPI CACGTG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 3 cut(s) 136, 491, 868
BccI CCATC 3 cut(s) 257, 391, 409
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BfmI CTRYAG 1 cut(s) 507
BfrI CTTAAG 1 cut(s) 685
BisI GCNGC 5 cut(s) 150, 153, 480, 483, 882
BlsI GCNGC 5 cut(s) 151, 154, 481, 484, 883
Bme18I GGWCC 1 cut(s) 700
BmgBI CACGTC 1 cut(s) 452
BmgT120I GGNCC 2 cut(s) 700, 799
BmsI GCATC 2 cut(s) 85, 341
BpiI GAAGAC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 828
Bsa29I ATCGAT 1 cut(s) 469
BsaAI YACGTR 3 cut(s) 260, 428, 785
BsaJI CCNNGG 1 cut(s) 500
BsaWI WCCGGW 2 cut(s) 397, 724
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 858
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 397
BseCI ATCGAT 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 500
BseLI CCNNNNNNNGG 2 cut(s) 107, 858
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 3 cut(s) 136, 491, 868
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 4 cut(s) 5, 505, 801, 862
BshVI ATCGAT 1 cut(s) 469
BsiSI CCGG 3 cut(s) 398, 725, 878
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 2 cut(s) 107, 858
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 4 cut(s) 5, 505, 801, 862
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 3 cut(s) 445, 518, 607
BspACI CCGC 3 cut(s) 152, 483, 682
BspANI GGCC 4 cut(s) 5, 505, 801, 862
BspDI ATCGAT 1 cut(s) 469
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 1 cut(s) 296
BspPI GGATC 1 cut(s) 440
BspQI GCTCTTC 1 cut(s) 263
BspTI CTTAAG 1 cut(s) 685
BsrFI RCCGGY 1 cut(s) 877
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 1 cut(s) 500
BssMI GATC 3 cut(s) 445, 518, 607
BssT1I CCWWGG 1 cut(s) 500
Bst4CI ACNGT 4 cut(s) 129, 166, 722, 750
Bst6I CTCTTC 2 cut(s) 263, 280
BstAFI CTTAAG 1 cut(s) 685
BstAPI GCANNNNNTGC 1 cut(s) 149
BstBAI YACGTR 3 cut(s) 260, 428, 785
BstC8I GCNNGC 4 cut(s) 198, 213, 372, 879
BstDEI CTNAG 2 cut(s) 458, 891
BstFNI CGCG 1 cut(s) 296
BstKTI GATC 3 cut(s) 448, 521, 610
BstMBI GATC 3 cut(s) 445, 518, 607
BstMWI GCNNNNNNNGC 2 cut(s) 149, 388
BstNSI RCATGY 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 507
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 3 cut(s) 136, 491, 868
BstV2I GAAGAC 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 469
BsuRI GGCC 4 cut(s) 5, 505, 801, 862
BsuTUI ATCGAT 1 cut(s) 469
BtrI CACGTC 1 cut(s) 452
Cac8I GCNNGC 4 cut(s) 198, 213, 372, 879
Cfr10I RCCGGY 1 cut(s) 877
Cfr13I GGNCC 2 cut(s) 700, 799
ClaI ATCGAT 1 cut(s) 469
CseI GACGC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 257
CviAII CATG 2 cut(s) 197, 704
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 2 cut(s) 458, 891
DpnI GATC 3 cut(s) 447, 520, 609
DpnII GATC 3 cut(s) 445, 518, 607
DraI TTTAAA 1 cut(s) 340
DraIII CACNNNGTG 1 cut(s) 566
DrdI GACNNNNNNGTC 1 cut(s) 847
DseDI GACNNNNNNGTC 1 cut(s) 847
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 263, 280
EarI CTCTTC 2 cut(s) 263, 280
Eco130I CCWWGG 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 700
Eco57I CTGAAG 1 cut(s) 80
Eco72I CACGTG 1 cut(s) 785
EcoT14I CCWWGG 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 500
FaeI CATG 2 cut(s) 200, 707
FaqI GGGAC 1 cut(s) 835
FatI CATG 2 cut(s) 196, 703
FauNDI CATATG 1 cut(s) 637
Fnu4HI GCNGC 5 cut(s) 150, 153, 480, 483, 882
Fsp4HI GCNGC 5 cut(s) 150, 153, 480, 483, 882
GluI GCNGC 5 cut(s) 150, 153, 480, 483, 882
GsaI CCCAGC 1 cut(s) 219
HaeIII GGCC 4 cut(s) 5, 505, 801, 862
HapII CCGG 3 cut(s) 398, 725, 878
HgaI GACGC 1 cut(s) 302
Hin1II CATG 2 cut(s) 200, 707
HinfI GANTC 5 cut(s) 471, 673, 763, 841, 848
HpaII CCGG 3 cut(s) 398, 725, 878
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 4 cut(s) 67, 250, 292, 364
Hpy188I TCNGA 6 cut(s) 60, 110, 549, 604, 646, 672
Hpy188III TCNNGA 2 cut(s) 398, 845
Hpy8I GTNNAC 4 cut(s) 67, 250, 292, 364
HpyAV CCTTC 2 cut(s) 55, 347
HpyCH4III ACNGT 4 cut(s) 129, 166, 722, 750
HpyCH4IV ACGT 5 cut(s) 259, 427, 436, 451, 784
HpyCH4V TGCA 4 cut(s) 98, 196, 560, 619
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 388
HpyF3I CTNAG 2 cut(s) 458, 891
HpySE526I ACGT 5 cut(s) 259, 427, 436, 451, 784
Hsp92II CATG 2 cut(s) 200, 707
Kpn2I TCCGGA 1 cut(s) 397
KroI GCCGGC 1 cut(s) 877
KroNI GCCGGC 1 cut(s) 879
Kzo9I GATC 3 cut(s) 445, 518, 607
LguI GCTCTTC 1 cut(s) 263
Lsp1109I GCAGC 3 cut(s) 136, 491, 868
LweI GCATC 2 cut(s) 85, 341
MaeII ACGT 5 cut(s) 259, 427, 436, 451, 784
MaeIII GTNAC 3 cut(s) 224, 744, 760
MalI GATC 3 cut(s) 447, 520, 609
MboI GATC 3 cut(s) 445, 518, 607
MboII GAAGA 6 cut(s) 280, 297, 359, 362, 431, 446
MfeI CAATTG 1 cut(s) 768
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 17, 234, 491, 768, 788, 864
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 757, 835, 857
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 3 cut(s) 63, 66, 116
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 397
MroNI GCCGGC 1 cut(s) 877
MroXI GAANNNNTTC 1 cut(s) 598
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 177, 312, 339, 686, 810
MslI CAYNNNNRTG 1 cut(s) 624
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 685
MspI CCGG 3 cut(s) 398, 725, 878
MunI CAATTG 1 cut(s) 768
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 2 cut(s) 149, 388
NaeI GCCGGC 1 cut(s) 879
NdeI CATATG 1 cut(s) 637
NdeII GATC 3 cut(s) 445, 518, 607
NgoMIV GCCGGC 1 cut(s) 877
NlaIII CATG 2 cut(s) 200, 707
NmuCI GTSAC 1 cut(s) 760
NspI RCATGY 1 cut(s) 200
PaeI GCATGC 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PdiI GCCGGC 1 cut(s) 879
PdmI GAANNNNTTC 1 cut(s) 598
PfeI GAWTC 2 cut(s) 471, 673
PkrI GCNGC 5 cut(s) 151, 154, 481, 484, 883
PleI GAGTC 3 cut(s) 757, 835, 856
PmaCI CACGTG 1 cut(s) 785
PmlI CACGTG 1 cut(s) 785
PpsI GAGTC 3 cut(s) 757, 835, 856
Ppu21I YACGTR 3 cut(s) 260, 428, 785
PspCI CACGTG 1 cut(s) 785
PspFI CCCAGC 1 cut(s) 215
PspPI GGNCC 2 cut(s) 700, 799
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 624
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 5 cut(s) 177, 312, 339, 686, 810
SatI GCNGC 5 cut(s) 150, 153, 480, 483, 882
Sau3AI GATC 3 cut(s) 445, 518, 607
Sau96I GGNCC 2 cut(s) 700, 799
SchI GAGTC 3 cut(s) 757, 835, 857
SfaNI GCATC 2 cut(s) 85, 341
SfcI CTRYAG 1 cut(s) 507
SinI GGWCC 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 624
SmlI CTYRAG 2 cut(s) 685, 843
SmoI CTYRAG 2 cut(s) 685, 843
SphI GCATGC 1 cut(s) 200
Sse9I AATT 6 cut(s) 17, 234, 491, 768, 788, 864
SsiI CCGC 3 cut(s) 152, 483, 682
StyI CCWWGG 1 cut(s) 500
TaaI ACNGT 4 cut(s) 129, 166, 722, 750
TaiI ACGT 5 cut(s) 262, 430, 439, 454, 787
TaqI TCGA 3 cut(s) 283, 413, 469
TasI AATT 6 cut(s) 17, 234, 491, 768, 788, 864
TauI GCSGC 2 cut(s) 155, 485
TfiI GAWTC 2 cut(s) 471, 673
Tru1I TTAA 5 cut(s) 177, 312, 339, 686, 810
Tru9I TTAA 5 cut(s) 177, 312, 339, 686, 810
TseFI GTSAC 1 cut(s) 760
TseI GCWGC 3 cut(s) 149, 479, 881
Tsp45I GTSAC 1 cut(s) 760
Vha464I CTTAAG 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 700
XapI RAATTY 2 cut(s) 491, 788
XceI RCATGY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.