Rh1BG226900

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003309
Physical Location & Seq
Reverse (-)
34670390 .. 34675002
4613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG226900.1

Sequence Viewer

Length: 942 bp
ATGCAGTCAAAATCAATCTGGCATGCTGATAAAGCTTTTGAGACATGCTCTCTTGTTGAGAATATATTGCATTTGGAGCCAATTGAAGCAAATATATTTCAGGGGATTACTTTACCGGAGGCACCAAGATTTGGACAGGTTTTCGGAGGACAGTTAGTTGGACAGGCATTGGCTGCAGCAACAAAAACTGTTGATTGTCTCAAAGTCGTCCATAGTTTGCATGCACATTTTTTGCTTGTCGGGGATTTCAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGAAAGAGCTTTGCTACTCGAAGAGTAGATGCAATACAAAAAGGGATAAACATATTCACTCTACTGGCTTCCTTTCAAAAAGAAGAAGAAGGGTCTATCCACCAGGAAGCCATAATGCCATCTGTGCCTCCCCCAGATACGCTCCCATCAATGGAAGAACTGCGCGAAAGACGCCTTTTTGATCCCCGTCTTCCAAGAACTTATCGAATCAAGGTAGCTGCAAAGGAGTTTGTCCCGTGGCCAATTGAGATACGATTTTGTGAACCTAGTAGCCGGACCAATCAGACTAAATCTCCACCAAGATTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTGGCTTATGCTTCAGATTTGATATTCGCTGGTGTAAGTCTGAATCCTCACCGTAGAAGAGGACATAAAACAAGTCAAGTTAGTTTGGACCATGCATTGTGGTTTCACCGCCCCCTTAGAGCTGATGACTGGTTACTACATGTGATAGTGAGTCCCATTGCCTACAATGCACGCGGCTATGTCACAGGCCAAATGTTCAATACCAAGGGAGAGCTTGTTGTATCATTGACTCAAGTGTCCCTGAATAGGCCAATTAGACCAACTCCGACTGTCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

313

Amino Acids

35.47

Weight (kDa)

9.69

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 43 - 122 4.8e-18 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 186 - 298 1.7e-23 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 190 - 296 4e-23 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 889
AccB1I GGYRCC 1 cut(s) 121
AccB7I CCANNNNNTGG 1 cut(s) 131
AccII CGCG 2 cut(s) 453, 828
AciI CCGC 2 cut(s) 763, 828
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 527
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 460
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 3 cut(s) 131, 439, 900
AflIII ACRYGT 1 cut(s) 793
AgsI TTSAA 4 cut(s) 86, 250, 365, 853
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 5 cut(s) 35, 297, 506, 776, 868
AluI AGCT 5 cut(s) 35, 297, 506, 776, 868
Alw26I GTCTC 2 cut(s) 35, 203
AlwI GGATC 1 cut(s) 464
AoxI GGCC 3 cut(s) 527, 841, 902
ApeKI GCWGC 3 cut(s) 173, 176, 506
Asp700I GAANNNNTTC 1 cut(s) 622
AspLEI GCGC 1 cut(s) 453
AspS9I GGNCC 2 cut(s) 564, 742
AsuHPI GGTGA 3 cut(s) 266, 695, 752
AvaII GGWCC 2 cut(s) 564, 742
BalI TGGCCA 1 cut(s) 529
BanI GGYRCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 470
BbvI GCAGC 3 cut(s) 160, 188, 493
BccI CCATC 3 cut(s) 281, 415, 442
BciT130I CCWGG 1 cut(s) 392
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 2 cut(s) 35, 203
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 4 cut(s) 174, 177, 507, 829
BlsI GCNGC 4 cut(s) 175, 178, 508, 830
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 2 cut(s) 564, 742
BmgT120I GGNCC 2 cut(s) 564, 742
BmiI GGNNCC 2 cut(s) 78, 123
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 307
BpiI GAAGAC 1 cut(s) 470
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpuEI CTTGAG 1 cut(s) 870
BsaAI YACGTR 1 cut(s) 284
BsaHI GRCGYC 1 cut(s) 460
BsaJI CCNNGG 2 cut(s) 524, 858
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 4 cut(s) 565, 595, 855, 885
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 439, 900
Bse1I ACTGG 2 cut(s) 357, 788
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 524, 858
BseLI CCNNNNNNNGG 3 cut(s) 131, 439, 900
BseMI GCAATG 1 cut(s) 810
BseNI ACTGG 2 cut(s) 357, 788
BseXI GCAGC 3 cut(s) 160, 188, 493
Bsh1236I CGCG 2 cut(s) 453, 828
BshFI GGCC 3 cut(s) 529, 843, 904
BshNI GGYRCC 1 cut(s) 121
BsiSI CCGG 2 cut(s) 116, 562
BslFI GGGAC 3 cut(s) 506, 792, 877
BslI CCNNNNNNNGG 3 cut(s) 131, 439, 900
BsmAI GTCTC 2 cut(s) 35, 203
BsmFI GGGAC 3 cut(s) 506, 792, 877
BsnI GGCC 3 cut(s) 529, 843, 904
Bsp143I GATC 2 cut(s) 469, 631
BspACI CCGC 2 cut(s) 763, 828
BspANI GGCC 3 cut(s) 529, 843, 904
BspFNI CGCG 2 cut(s) 453, 828
BspLI GGNNCC 2 cut(s) 78, 123
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 2 cut(s) 357, 788
BssECI CCNNGG 2 cut(s) 524, 858
BssMI GATC 2 cut(s) 469, 631
BssNI GRCGYC 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 858
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 4 cut(s) 153, 190, 707, 925
Bst6I CTCTTC 2 cut(s) 304, 706
BstACI GRCGYC 1 cut(s) 460
BstAPI GCANNNNNTGC 1 cut(s) 173
BstBAI YACGTR 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 24, 222, 826
BstDEI CTNAG 2 cut(s) 770, 933
BstDSI CCRYGG 1 cut(s) 524
BstFNI CGCG 2 cut(s) 453, 828
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 2 cut(s) 472, 634
BstMAI GTCTC 2 cut(s) 35, 203
BstMBI GATC 2 cut(s) 469, 631
BstMWI GCNNNNNNNGC 6 cut(s) 32, 76, 173, 412, 459, 821
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 4 cut(s) 26, 48, 224, 797
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 174
BstUI CGCG 2 cut(s) 453, 828
BstV1I GCAGC 3 cut(s) 160, 188, 493
BstV2I GAAGAC 1 cut(s) 470
BsuRI GGCC 3 cut(s) 529, 843, 904
BtgI CCRYGG 1 cut(s) 524
Cac8I GCNNGC 3 cut(s) 24, 222, 826
CfoI GCGC 1 cut(s) 453
Cfr13I GGNCC 2 cut(s) 564, 742
CseI GACGC 1 cut(s) 468
Csp6I GTAC 1 cut(s) 281
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 5 cut(s) 23, 45, 221, 746, 794
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 2 cut(s) 770, 933
DpnI GATC 2 cut(s) 471, 633
DpnII GATC 2 cut(s) 469, 631
DrdI GACNNNNNNGTC 1 cut(s) 889
DseDI GACNNNNNNGTC 1 cut(s) 889
EaeI YGGCCR 1 cut(s) 527
Eam1104I CTCTTC 2 cut(s) 304, 706
EarI CTCTTC 2 cut(s) 304, 706
Eco130I CCWWGG 1 cut(s) 858
Eco47I GGWCC 2 cut(s) 564, 742
Eco57I CTGAAG 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 1 cut(s) 751
ErhI CCWWGG 1 cut(s) 858
FaeI CATG 5 cut(s) 26, 48, 224, 749, 797
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 3 cut(s) 506, 792, 877
FatI CATG 5 cut(s) 22, 44, 220, 745, 793
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 4 cut(s) 174, 177, 507, 829
Fsp4HI GCNGC 4 cut(s) 174, 177, 507, 829
FspBI CTAG 1 cut(s) 555
GlaI GCGC 1 cut(s) 452
GluI GCNGC 4 cut(s) 174, 177, 507, 829
HaeIII GGCC 3 cut(s) 529, 843, 904
HapII CCGG 2 cut(s) 116, 562
HgaI GACGC 1 cut(s) 468
HhaI GCGC 1 cut(s) 453
Hin1I GRCGYC 1 cut(s) 460
Hin1II CATG 5 cut(s) 26, 48, 224, 749, 797
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 33
HinfI GANTC 4 cut(s) 495, 697, 805, 883
HpaII CCGG 2 cut(s) 116, 562
HphI GGTGA 3 cut(s) 266, 695, 752
Hpy166II GTNNAC 2 cut(s) 274, 551
Hpy188I TCNGA 6 cut(s) 146, 573, 628, 670, 696, 921
Hpy8I GTNNAC 2 cut(s) 274, 551
HpyAV CCTTC 1 cut(s) 371
HpyCH4III ACNGT 4 cut(s) 153, 190, 707, 925
HpyCH4IV ACGT 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 76, 173, 412, 459, 821
HpyF3I CTNAG 2 cut(s) 770, 933
HpySE526I ACGT 1 cut(s) 283
Hsp92I GRCGYC 1 cut(s) 460
Hsp92II CATG 5 cut(s) 26, 48, 224, 749, 797
HspAI GCGC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 2 cut(s) 469, 631
LmnI GCTCC 2 cut(s) 76, 435
Lsp1109I GCAGC 3 cut(s) 160, 188, 493
LweI GCATC 1 cut(s) 307
MaeI CTAG 1 cut(s) 555
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 3 cut(s) 786, 835, 925
MalI GATC 2 cut(s) 471, 633
MboI GATC 2 cut(s) 469, 631
MboII GAAGA 6 cut(s) 321, 383, 386, 455, 470, 723
MfeI CAATTG 2 cut(s) 81, 531
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 4 cut(s) 81, 258, 531, 906
MluNI TGGCCA 1 cut(s) 529
MlyI GAGTC 2 cut(s) 814, 877
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 6 cut(s) 112, 140, 426, 588, 707, 711
Mox20I TGGCCA 1 cut(s) 529
Mph1103I ATGCAT 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 622
MscI TGGCCA 1 cut(s) 529
MslI CAYNNNNRTG 1 cut(s) 648
Msp20I TGGCCA 1 cut(s) 529
MspI CCGG 2 cut(s) 116, 562
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 2 cut(s) 81, 531
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 2 cut(s) 453, 828
MwoI GCNNNNNNNGC 6 cut(s) 32, 76, 173, 412, 459, 821
NdeII GATC 2 cut(s) 469, 631
NlaIII CATG 5 cut(s) 26, 48, 224, 749, 797
NlaIV GGNNCC 2 cut(s) 78, 123
NmuCI GTSAC 2 cut(s) 835, 925
NsiI ATGCAT 1 cut(s) 751
NspI RCATGY 4 cut(s) 26, 48, 224, 797
PaeI GCATGC 2 cut(s) 26, 224
PciI ACATGT 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 2 cut(s) 495, 697
PflMI CCANNNNNTGG 1 cut(s) 131
PkrI GCNGC 4 cut(s) 175, 178, 508, 830
PleI GAGTC 2 cut(s) 813, 877
PpsI GAGTC 2 cut(s) 813, 877
Ppu21I YACGTR 1 cut(s) 284
PscI ACATGT 1 cut(s) 793
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 78, 123
PspPI GGNCC 2 cut(s) 564, 742
PstI CTGCAG 1 cut(s) 178
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
RseI CAYNNNNRTG 1 cut(s) 648
SatI GCNGC 4 cut(s) 174, 177, 507, 829
Sau3AI GATC 2 cut(s) 469, 631
Sau96I GGNCC 2 cut(s) 564, 742
SchI GAGTC 2 cut(s) 814, 877
ScrFI CCNGG 1 cut(s) 392
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 2 cut(s) 564, 742
SmiMI CAYNNNNRTG 1 cut(s) 648
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
SphI GCATGC 2 cut(s) 26, 224
Sse9I AATT 4 cut(s) 81, 258, 531, 906
SsiI CCGC 2 cut(s) 763, 828
SspMI CTAG 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 4 cut(s) 153, 190, 707, 925
TaiI ACGT 1 cut(s) 286
TaqI TCGA 2 cut(s) 307, 493
TasI AATT 4 cut(s) 81, 258, 531, 906
TauI GCSGC 1 cut(s) 831
TfiI GAWTC 2 cut(s) 495, 697
TseFI GTSAC 2 cut(s) 835, 925
TseI GCWGC 3 cut(s) 173, 176, 506
Tsp45I GTSAC 2 cut(s) 835, 925
Van91I CCANNNNNTGG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 564, 742
XceI RCATGY 4 cut(s) 26, 48, 224, 797
XmnI GAANNNNTTC 1 cut(s) 622
XspI CTAG 1 cut(s) 555
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.