Rh4BG185900

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003309
Physical Location & Seq
Reverse (-)
32490670 .. 32511103
20434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG185900.1

Sequence Viewer

Length: 660 bp
ATGATGAAGAGAAGAGAGAAGAGAGCGGACCTACGCAGGAAAGAAAATCAAACGCTAGTGGCGCTGGTTGAGTCGGAGAACAAGCTGAGGTCGGGGGCGCGGGTCAGGTTGGAGAAGGAGCTGGGTCAGGTTGGAGAAGGAGCTGGGGTCGGGGACGTGGAGAATGAGGCAGTCAAAAGGTCTTCGCCATTGAAGCTCCCTCTCTTCCATATCTATCACCTTCCCATTTCAGTGATTGAGTTTCTGGGCTGCGTTCCTCTGCTTCAGCGCCTACCCAGCTCCTCGCTCAAGAAAATCGCCCAGCTCGTAATTCCAAAGCACTACGATAAAGGAGAGTATGTTGTTCGTGAAGGTAAGGATGGGATTGGGGTTTACTTCATTTGGGAAGGAGAGCAGGAAGGCGTCCCTGTCTTCCAATGGTGGTCGGCTGCAGCTCACGTGACGAGCTCGACGCCGTCGGCTCTGCCGTCACAACCGCCCCCTATATCTCTTTATCCCCCCTGTTGTCAAATTGCTGGGGAGTGGACAATATATCCGTGGATCCACAGCAATTATATTCATCCCGCTAGTGCAGGGATTACTGAAGGCTTTTCTATGTGTGGCGGTGACTTTGTTGAGGTTTATAACGATCCAAGCCAAGTAGGAACATATATGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

219

Amino Acids

24.39

Weight (kDa)

6.6

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AccBSI CCGCTC 1 cut(s) 26
AccII CGCG 1 cut(s) 100
AciI CCGC 5 cut(s) 26, 100, 476, 564, 603
AclWI GGATC 3 cut(s) 535, 548, 623
AcuI CTGAAG 2 cut(s) 248, 603
AcvI CACGTG 1 cut(s) 439
AcyI GRCGYC 2 cut(s) 402, 452
AgsI TTSAA 1 cut(s) 193
AjiI CACGTC 1 cut(s) 157
AluBI AGCT 8 cut(s) 85, 121, 143, 196, 279, 304, 434, 447
AluI AGCT 8 cut(s) 85, 121, 143, 196, 279, 304, 434, 447
Alw21I GWGCWC 1 cut(s) 449
AlwI GGATC 3 cut(s) 535, 548, 623
ApeKI GCWGC 3 cut(s) 249, 428, 431
AspLEI GCGC 3 cut(s) 64, 100, 270
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 209, 617
AvaII GGWCC 1 cut(s) 28
BamHI GGATCC 1 cut(s) 540
BanII GRGCYC 1 cut(s) 449
BbrPI CACGTG 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 174, 403
Bbv12I GWGCWC 1 cut(s) 449
BbvCI CCTCAGC 1 cut(s) 86
BbvI GCAGC 3 cut(s) 236, 415, 443
BccI CCATC 1 cut(s) 353
BceAI ACGGC 2 cut(s) 439, 451
BfaI CTAG 2 cut(s) 56, 567
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 2 cut(s) 65, 271
BisI GCNGC 3 cut(s) 250, 429, 432
BlsI GCNGC 3 cut(s) 251, 430, 433
Bme18I GGWCC 1 cut(s) 28
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 542
BpiI GAAGAC 2 cut(s) 174, 403
Bpu10I CCTNAGC 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 439
BsaHI GRCGYC 2 cut(s) 402, 452
BsaJI CCNNGG 1 cut(s) 536
BsaXI ACNNNNNCTCC 4 cut(s) 132, 162, 324, 354
BseDI CCNNGG 1 cut(s) 536
BseGI GGATG 2 cut(s) 364, 559
BseMII CTCAG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 271
BseXI GCAGC 3 cut(s) 236, 415, 443
BseYI CCCAGC 5 cut(s) 121, 143, 275, 300, 515
BsgI GTGCAG 1 cut(s) 591
Bsh1236I CGCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 449
BslFI GGGAC 2 cut(s) 167, 389
BsmFI GGGAC 2 cut(s) 167, 389
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 2 cut(s) 540, 628
BspACI CCGC 5 cut(s) 26, 100, 476, 564, 603
BspCNI CTCAG 1 cut(s) 78
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 542
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 3 cut(s) 535, 548, 623
BsrBI CCGCTC 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 536
BssMI GATC 2 cut(s) 540, 628
BssNI GRCGYC 2 cut(s) 402, 452
Bst6I CTCTTC 4 cut(s) 2, 7, 14, 209
BstACI GRCGYC 2 cut(s) 402, 452
BstBAI YACGTR 1 cut(s) 439
BstDEI CTNAG 1 cut(s) 86
BstDSI CCRYGG 1 cut(s) 536
BstF5I GGATG 2 cut(s) 364, 559
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 65, 271
BstHHI GCGC 3 cut(s) 64, 100, 270
BstKTI GATC 2 cut(s) 543, 631
BstMBI GATC 2 cut(s) 540, 628
BstMWI GCNNNNNNNGC 3 cut(s) 61, 193, 276
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 3 cut(s) 236, 415, 443
BstV2I GAAGAC 2 cut(s) 174, 403
BstX2I RGATCY 1 cut(s) 540
BstYI RGATCY 1 cut(s) 540
BtgI CCRYGG 1 cut(s) 536
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 2 cut(s) 364, 559
BtsIMutI CAGTG 1 cut(s) 237
CfoI GCGC 3 cut(s) 64, 100, 270
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 2 cut(s) 391, 460
DdeI CTNAG 1 cut(s) 86
DpnI GATC 2 cut(s) 542, 630
DpnII GATC 2 cut(s) 540, 628
Eam1104I CTCTTC 4 cut(s) 2, 7, 14, 209
EarI CTCTTC 4 cut(s) 2, 7, 14, 209
Ecl136II GAGCTC 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 449
Eco47I GGWCC 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 2 cut(s) 248, 603
Eco72I CACGTG 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 449
FaqI GGGAC 2 cut(s) 167, 389
FauI CCCGC 2 cut(s) 93, 571
Fnu4HI GCNGC 3 cut(s) 250, 429, 432
FokI GGATG 2 cut(s) 371, 546
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 3 cut(s) 250, 429, 432
FspBI CTAG 2 cut(s) 56, 567
GlaI GCGC 3 cut(s) 63, 99, 269
GluI GCNGC 3 cut(s) 250, 429, 432
GsaI CCCAGC 5 cut(s) 125, 147, 279, 304, 519
HaeII RGCGCY 2 cut(s) 65, 271
HgaI GACGC 2 cut(s) 391, 460
HhaI GCGC 3 cut(s) 64, 100, 270
Hin1I GRCGYC 2 cut(s) 402, 452
Hin6I GCGC 3 cut(s) 62, 98, 268
HinP1I GCGC 3 cut(s) 62, 98, 268
HinfI GANTC 1 cut(s) 71
HphI GGTGA 2 cut(s) 209, 617
Hpy166II GTNNAC 2 cut(s) 373, 525
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 2 cut(s) 289, 347
Hpy8I GTNNAC 2 cut(s) 373, 525
Hpy99I CGWCG 2 cut(s) 454, 460
HpyAV CCTTC 7 cut(s) 109, 131, 230, 344, 380, 392, 578
HpyCH4IV ACGT 2 cut(s) 156, 438
HpyCH4V TGCA 2 cut(s) 431, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 193, 276
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 2 cut(s) 156, 438
Hsp92I GRCGYC 2 cut(s) 402, 452
HspAI GCGC 3 cut(s) 62, 98, 268
Kzo9I GATC 2 cut(s) 540, 628
LmnI GCTCC 4 cut(s) 118, 140, 201, 284
Lsp1109I GCAGC 3 cut(s) 236, 415, 443
MaeI CTAG 2 cut(s) 56, 567
MaeII ACGT 2 cut(s) 156, 438
MaeIII GTNAC 3 cut(s) 439, 468, 605
MalI GATC 2 cut(s) 542, 630
MbiI CCGCTC 1 cut(s) 26
MboI GATC 2 cut(s) 540, 628
MboII GAAGA 6 cut(s) 19, 24, 31, 174, 196, 403
MflI RGATCY 1 cut(s) 540
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 3 cut(s) 309, 510, 550
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 3 cut(s) 54, 90, 112
MnlI CCTC 6 cut(s) 81, 160, 210, 267, 292, 610
MslI CAYNNNNRTG 1 cut(s) 230
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 61, 193, 276
NdeII GATC 2 cut(s) 540, 628
NlaIV GGNNCC 1 cut(s) 542
NmuCI GTSAC 3 cut(s) 439, 468, 605
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 464
PflFI GACNNNGTC 1 cut(s) 454
PkrI GCNGC 3 cut(s) 251, 430, 433
PleI GAGTC 1 cut(s) 79
PmaCI CACGTG 1 cut(s) 439
PmlI CACGTG 1 cut(s) 439
PpsI GAGTC 1 cut(s) 79
Ppu21I YACGTR 1 cut(s) 439
PsiI TTATAA 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 449
PspCI CACGTG 1 cut(s) 439
PspFI CCCAGC 5 cut(s) 121, 143, 275, 300, 515
PspN4I GGNNCC 1 cut(s) 542
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 540
PsyI GACNNNGTC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 230
SacI GAGCTC 1 cut(s) 449
SatI GCNGC 3 cut(s) 250, 429, 432
Sau3AI GATC 2 cut(s) 540, 628
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 80
SduI GDGCHC 1 cut(s) 449
SfcI CTRYAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 3 cut(s) 309, 510, 550
SsiI CCGC 5 cut(s) 26, 100, 476, 564, 603
SspMI CTAG 2 cut(s) 56, 567
SstI GAGCTC 1 cut(s) 449
TaiI ACGT 2 cut(s) 159, 441
TaqI TCGA 1 cut(s) 449
TasI AATT 3 cut(s) 309, 510, 550
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 3 cut(s) 439, 468, 605
TseI GCWGC 3 cut(s) 249, 428, 431
Tsp45I GTSAC 3 cut(s) 439, 468, 605
TspDTI ATGAA 3 cut(s) 20, 367, 548
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 1 cut(s) 237
Tth111I GACNNNGTC 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 28
XspI CTAG 2 cut(s) 56, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.