Rh1DG254500

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003309
Physical Location & Seq
Reverse (-)
46834501 .. 46840598
6098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG254500.1

Sequence Viewer

Length: 990 bp
ATGGAATTGGCAGTACTAGAGCCATTAGAGAATGGAGACGGTTTTGTTCCAGAGTCAAAATCAATCTGGCATGCTGATAAAGCTTTTGAGACATGCTCTCTTGTTGAGAATATATTGCATTTGGAGCCAATTGAAGCAAATATATTTCAGGGGATTACTTTACCGGAGGCACCAAGATTTGGACAGGTTTTCGGAGGACAGTTAGTTGGACAGGCATTGGCTGCAGCAACAAAAACTGTTGATTGTCTCAAAGTCGTCCATAGTTTGCATGCACATTTTTTGCTTGTCGGGGATTTCAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGAAAGAGCTTTGCTACTCGAAGAGTAGATGCAATACAAAAAGGGATAAACATATTCACTCTACTGGCTTCCTTTCAAAAAGAAGAAGAAGGGTCTATCCACCAGGAAGCCATAATGCCATCTGTGCCTCCCCCAGATACGCTCCCATCAATGGAAGAACTGCGCGAAAGACGCCTTTTTGATCCCCGTCTTCCAAGAACTTATCGAATCAAGGTAGCTGCAAAGGAGTTTGTCCCGTGGCCAATTGAGATACGATTTTGTGAACCTAGTAGCCGGACCAATCAGACTAAATCTCCACCAAGATTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTGGCTTATGCTTCAGATTTGATATTCGCTGGTGTAAGTCTGAATCCTCACCGTAGAAGAGGACATAAAACAAGTCAAGTTAGTTTGGACCATGCATTGTGGTTTCACCGCCCCCTTAGAGCTGATGACTGGTTACTACATGTGATAGTGAGTCCCATTGCCTACAATGCACGCGGCTATGTCACAGGCCAAATGTTCAATACCAAGGGAGAGCTTGTTGTATCATTGACTCAAGTGTCCCTGAATAGGCCAATTAGACCAACTCCGACTGTCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

329

Amino Acids

37.15

Weight (kDa)

9.17

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 59 - 138 5.3e-18 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 203 - 314 1.9e-23 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 206 - 312 4.5e-23 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 937
AccB1I GGYRCC 1 cut(s) 169
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 2 cut(s) 501, 876
AciI CCGC 2 cut(s) 811, 876
AclWI GGATC 1 cut(s) 512
AcoI YGGCCR 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 699
AcyI GRCGYC 1 cut(s) 508
AfaI GTAC 2 cut(s) 15, 330
AfiI CCNNNNNNNGG 3 cut(s) 179, 487, 948
AflIII ACRYGT 1 cut(s) 841
AgsI TTSAA 4 cut(s) 134, 298, 413, 901
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 5 cut(s) 83, 345, 554, 824, 916
AluI AGCT 5 cut(s) 83, 345, 554, 824, 916
Alw26I GTCTC 3 cut(s) 30, 83, 251
AlwI GGATC 1 cut(s) 512
AoxI GGCC 3 cut(s) 575, 889, 950
ApeKI GCWGC 3 cut(s) 221, 224, 554
Asp700I GAANNNNTTC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 2 cut(s) 612, 790
AsuHPI GGTGA 3 cut(s) 314, 743, 800
AvaII GGWCC 2 cut(s) 612, 790
BalI TGGCCA 1 cut(s) 577
BanI GGYRCC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 518
BbvI GCAGC 3 cut(s) 208, 236, 541
BccI CCATC 3 cut(s) 329, 463, 490
BciT130I CCWGG 1 cut(s) 440
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 30, 83, 251
BfaI CTAG 2 cut(s) 17, 603
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 4 cut(s) 222, 225, 555, 877
BlsI GCNGC 4 cut(s) 223, 226, 556, 878
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 1 cut(s) 440
Bme18I GGWCC 2 cut(s) 612, 790
BmgT120I GGNCC 2 cut(s) 612, 790
BmiI GGNNCC 2 cut(s) 126, 171
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 518
BplI GAGNNNNNCTC 2 cut(s) 80, 112
BpuEI CTTGAG 1 cut(s) 918
BsaAI YACGTR 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 508
BsaJI CCNNGG 2 cut(s) 572, 906
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 4 cut(s) 613, 643, 903, 933
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 487, 948
Bse1I ACTGG 2 cut(s) 405, 836
Bse3DI GCAATG 1 cut(s) 858
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 572, 906
BseLI CCNNNNNNNGG 3 cut(s) 179, 487, 948
BseMI GCAATG 1 cut(s) 858
BseNI ACTGG 2 cut(s) 405, 836
BseXI GCAGC 3 cut(s) 208, 236, 541
Bsh1236I CGCG 2 cut(s) 501, 876
BshFI GGCC 3 cut(s) 577, 891, 952
BshNI GGYRCC 1 cut(s) 169
BsiSI CCGG 2 cut(s) 164, 610
BslFI GGGAC 3 cut(s) 554, 840, 925
BslI CCNNNNNNNGG 3 cut(s) 179, 487, 948
BsmAI GTCTC 3 cut(s) 30, 83, 251
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 3 cut(s) 554, 840, 925
BsnI GGCC 3 cut(s) 577, 891, 952
Bsp143I GATC 2 cut(s) 517, 679
BspACI CCGC 2 cut(s) 811, 876
BspANI GGCC 3 cut(s) 577, 891, 952
BspFNI CGCG 2 cut(s) 501, 876
BspLI GGNNCC 2 cut(s) 126, 171
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 858
BsrI ACTGG 2 cut(s) 405, 836
BssECI CCNNGG 2 cut(s) 572, 906
BssMI GATC 2 cut(s) 517, 679
BssNI GRCGYC 1 cut(s) 508
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 5 cut(s) 41, 201, 238, 755, 973
Bst6I CTCTTC 2 cut(s) 352, 754
BstACI GRCGYC 1 cut(s) 508
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 332
BstC8I GCNNGC 3 cut(s) 72, 270, 874
BstDEI CTNAG 2 cut(s) 818, 981
BstDSI CCRYGG 1 cut(s) 572
BstFNI CGCG 2 cut(s) 501, 876
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 2 cut(s) 520, 682
BstMAI GTCTC 3 cut(s) 30, 83, 251
BstMBI GATC 2 cut(s) 517, 679
BstMWI GCNNNNNNNGC 6 cut(s) 80, 124, 221, 460, 507, 869
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 4 cut(s) 74, 96, 272, 845
BstSCI CCNGG 1 cut(s) 438
BstSFI CTRYAG 1 cut(s) 222
BstUI CGCG 2 cut(s) 501, 876
BstV1I GCAGC 3 cut(s) 208, 236, 541
BstV2I GAAGAC 1 cut(s) 518
BsuRI GGCC 3 cut(s) 577, 891, 952
BtgI CCRYGG 1 cut(s) 572
Cac8I GCNNGC 3 cut(s) 72, 270, 874
CfoI GCGC 1 cut(s) 501
Cfr13I GGNCC 2 cut(s) 612, 790
CseI GACGC 1 cut(s) 516
Csp6I GTAC 2 cut(s) 14, 329
CspCI CAANNNNNGTGG 2 cut(s) 621, 656
CviAII CATG 5 cut(s) 71, 93, 269, 794, 842
CviQI GTAC 2 cut(s) 14, 329
DdeI CTNAG 2 cut(s) 818, 981
DpnI GATC 2 cut(s) 519, 681
DpnII GATC 2 cut(s) 517, 679
DrdI GACNNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 937
EaeI YGGCCR 1 cut(s) 575
Eam1104I CTCTTC 2 cut(s) 352, 754
EarI CTCTTC 2 cut(s) 352, 754
Eco130I CCWWGG 1 cut(s) 906
Eco47I GGWCC 2 cut(s) 612, 790
Eco57I CTGAAG 1 cut(s) 699
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 906
EcoT22I ATGCAT 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 906
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 5 cut(s) 74, 96, 272, 797, 845
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 3 cut(s) 554, 840, 925
FatI CATG 5 cut(s) 70, 92, 268, 793, 841
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 4 cut(s) 222, 225, 555, 877
Fsp4HI GCNGC 4 cut(s) 222, 225, 555, 877
FspBI CTAG 2 cut(s) 17, 603
GlaI GCGC 1 cut(s) 500
GluI GCNGC 4 cut(s) 222, 225, 555, 877
HaeIII GGCC 3 cut(s) 577, 891, 952
HapII CCGG 2 cut(s) 164, 610
HgaI GACGC 1 cut(s) 516
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 508
Hin1II CATG 5 cut(s) 74, 96, 272, 797, 845
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 5 cut(s) 53, 543, 745, 853, 931
HpaII CCGG 2 cut(s) 164, 610
HphI GGTGA 3 cut(s) 314, 743, 800
Hpy166II GTNNAC 2 cut(s) 322, 599
Hpy188I TCNGA 6 cut(s) 194, 621, 676, 718, 744, 969
Hpy188III TCNNGA 1 cut(s) 50
Hpy8I GTNNAC 2 cut(s) 322, 599
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 5 cut(s) 41, 201, 238, 755, 973
HpyCH4IV ACGT 1 cut(s) 331
HpyCH4V TGCA 9 cut(s) 118, 224, 268, 272, 368, 557, 691, 797, 872
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 124, 221, 460, 507, 869
HpyF3I CTNAG 2 cut(s) 818, 981
HpySE526I ACGT 1 cut(s) 331
Hsp92I GRCGYC 1 cut(s) 508
Hsp92II CATG 5 cut(s) 74, 96, 272, 797, 845
HspAI GCGC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 2 cut(s) 517, 679
LmnI GCTCC 2 cut(s) 124, 483
Lsp1109I GCAGC 3 cut(s) 208, 236, 541
LweI GCATC 1 cut(s) 355
MaeI CTAG 2 cut(s) 17, 603
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 3 cut(s) 834, 883, 973
MalI GATC 2 cut(s) 519, 681
MboI GATC 2 cut(s) 517, 679
MboII GAAGA 6 cut(s) 369, 431, 434, 503, 518, 771
MfeI CAATTG 2 cut(s) 129, 579
MlsI TGGCCA 1 cut(s) 577
MluCI AATT 5 cut(s) 5, 129, 306, 579, 954
MluNI TGGCCA 1 cut(s) 577
MlyI GAGTC 3 cut(s) 62, 862, 925
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 6 cut(s) 160, 188, 474, 636, 755, 759
Mox20I TGGCCA 1 cut(s) 577
Mph1103I ATGCAT 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 670
MscI TGGCCA 1 cut(s) 577
MslI CAYNNNNRTG 1 cut(s) 696
Msp20I TGGCCA 1 cut(s) 577
MspI CCGG 2 cut(s) 164, 610
MspR9I CCNGG 1 cut(s) 440
MunI CAATTG 2 cut(s) 129, 579
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 2 cut(s) 501, 876
MwoI GCNNNNNNNGC 6 cut(s) 80, 124, 221, 460, 507, 869
NdeII GATC 2 cut(s) 517, 679
NlaIII CATG 5 cut(s) 74, 96, 272, 797, 845
NlaIV GGNNCC 2 cut(s) 126, 171
NmuCI GTSAC 2 cut(s) 883, 973
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 4 cut(s) 74, 96, 272, 845
PaeI GCATGC 2 cut(s) 74, 272
PciI ACATGT 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 670
PfeI GAWTC 2 cut(s) 543, 745
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 4 cut(s) 223, 226, 556, 878
PleI GAGTC 3 cut(s) 61, 861, 925
PpsI GAGTC 3 cut(s) 61, 861, 925
Ppu21I YACGTR 1 cut(s) 332
PscI ACATGT 1 cut(s) 841
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 126, 171
PspPI GGNCC 2 cut(s) 612, 790
PstI CTGCAG 1 cut(s) 226
RsaI GTAC 2 cut(s) 15, 330
RsaNI GTAC 2 cut(s) 14, 329
RseI CAYNNNNRTG 1 cut(s) 696
SatI GCNGC 4 cut(s) 222, 225, 555, 877
Sau3AI GATC 2 cut(s) 517, 679
Sau96I GGNCC 2 cut(s) 612, 790
ScaI AGTACT 1 cut(s) 15
SchI GAGTC 3 cut(s) 62, 862, 925
ScrFI CCNGG 1 cut(s) 440
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 2 cut(s) 612, 790
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 933
SmoI CTYRAG 1 cut(s) 933
SphI GCATGC 2 cut(s) 74, 272
Sse9I AATT 5 cut(s) 5, 129, 306, 579, 954
SsiI CCGC 2 cut(s) 811, 876
SspMI CTAG 2 cut(s) 17, 603
StyD4I CCNGG 1 cut(s) 438
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 5 cut(s) 41, 201, 238, 755, 973
TaiI ACGT 1 cut(s) 334
TaqI TCGA 2 cut(s) 355, 541
TasI AATT 5 cut(s) 5, 129, 306, 579, 954
TatI WGTACW 1 cut(s) 13
TauI GCSGC 1 cut(s) 879
TfiI GAWTC 2 cut(s) 543, 745
TseFI GTSAC 2 cut(s) 883, 973
TseI GCWGC 3 cut(s) 221, 224, 554
Tsp45I GTSAC 2 cut(s) 883, 973
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 612, 790
XceI RCATGY 4 cut(s) 74, 96, 272, 845
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 2 cut(s) 17, 603
ZrmI AGTACT 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.