pycom01g08790

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003309
Physical Location & Seq
Reverse (-)
9726907 .. 9730998
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g08790.4

Sequence Viewer

Length: 843 bp
ATGGCCACATATAAAGAATTGTTTTGGTTGATAAGATTCTATTCCTTCCCCCCTCCTCTGAAGGTAAACATATTTGAAGGGATTACTTTGCCAGATGCGCCAAGTTTCGAACAGGTTTTTGGAGGACAGTTAGTTGGACAGGCATTGGCTGCGGCAACGAAAACTGTTGATTGTCTTAAACTTCTCCATAGTGTGCATGCGCATTTTCTGCTGGCTGGGGATTGTAACACACCAATTATTTATCAAGTTCACAGAATACGTGATGGGAAGAGCTTTGCTACTCGAAGAGTGGACGCGATACAAAAGGGGATTAACATATTCACGTTACTGGCATCATTTAAGAAAGAAGAAGAAGGGTTTGTCCACCAGCAAGCAACAATGCCATCTGTGCCTGCTCCGGATAAACTTCTGTCAATGGAAGAACTACGTGAAAAACATCTGCTTGATCCACGTCTTCTAAGGACTTATCGATTCAATGCAGCTGCAAAGAAATTTGTGCCTTGGCCTATAGAGATACGATCTTGTGAGCCTAACAGCTCTACCAATCAGACTGAATCTGCACCAAGATGTGTTGTAGCATATGCTTCGGATTTGATATTTTTGGGTGTGAGTCTGAATCCCCAACGCTTAAGAGTTAGTTTGGACCATGCGATTTGGTTTCACCGTCCGGTTAGAGCTGATGACTGGTTACTGTATGTGATAGTGACTCCAATTGCCTACGACACGCGTGAATTTGTTTCGGGCCAAATGTTTAACATAAAGGGAGAGCTTATTGTATCATTGACTCAAGAGTCCCTAAATAGGCGAATTAGACCAACTCCGGCTGCCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

281

Amino Acids

31.75

Weight (kDa)

9.16

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 790
Acc16I TGCGCA 1 cut(s) 201
AccII CGCG 2 cut(s) 296, 727
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 1 cut(s) 152
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 491, 731
AcuI CTGAAG 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 801
AflII CTTAAG 1 cut(s) 628
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 2 cut(s) 77, 475
AjiI CACGTC 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 5 cut(s) 273, 482, 537, 677, 769
AluI AGCT 5 cut(s) 273, 482, 537, 677, 769
AlwI GGATC 1 cut(s) 440
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 3 cut(s) 3, 503, 742
ApeKI GCWGC 4 cut(s) 149, 479, 482, 824
ApoI RAATTY 2 cut(s) 491, 731
AspLEI GCGC 2 cut(s) 100, 202
AspS9I GGNCC 2 cut(s) 643, 742
AsuHPI GGTGA 1 cut(s) 653
AsuII TTCGAA 1 cut(s) 108
AvaII GGWCC 1 cut(s) 643
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 4 cut(s) 136, 469, 491, 811
BccI CCATC 2 cut(s) 257, 391
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BfmI CTRYAG 1 cut(s) 507
BfrI CTTAAG 1 cut(s) 628
BisI GCNGC 5 cut(s) 150, 153, 480, 483, 825
BlsI GCNGC 5 cut(s) 151, 154, 481, 484, 826
Bme18I GGWCC 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 452
BmgT120I GGNCC 2 cut(s) 643, 742
BmsI GCATC 2 cut(s) 85, 341
BpiI GAAGAC 1 cut(s) 446
Bpu14I TTCGAA 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 771
Bsa29I ATCGAT 1 cut(s) 469
BsaAI YACGTR 2 cut(s) 260, 428
BsaJI CCNNGG 1 cut(s) 500
BsaWI WCCGGW 2 cut(s) 397, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 2 cut(s) 333, 689
BseAI TCCGGA 1 cut(s) 397
BseCI ATCGAT 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 500
BseLI CCNNNNNNNGG 1 cut(s) 801
BseNI ACTGG 2 cut(s) 333, 689
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 4 cut(s) 136, 469, 491, 811
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 2 cut(s) 296, 727
BshFI GGCC 3 cut(s) 5, 505, 744
BshVI ATCGAT 1 cut(s) 469
BsiSI CCGG 3 cut(s) 398, 668, 821
BslFI GGGAC 1 cut(s) 778
BslI CCNNNNNNNGG 1 cut(s) 801
BsmFI GGGAC 1 cut(s) 778
BsnI GGCC 3 cut(s) 5, 505, 744
Bsp119I TTCGAA 1 cut(s) 108
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 2 cut(s) 445, 518
BspACI CCGC 1 cut(s) 152
BspANI GGCC 3 cut(s) 5, 505, 744
BspDI ATCGAT 1 cut(s) 469
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 2 cut(s) 296, 727
BspPI GGATC 1 cut(s) 440
BspQI GCTCTTC 1 cut(s) 263
BspT104I TTCGAA 1 cut(s) 108
BspTI CTTAAG 1 cut(s) 628
BsrI ACTGG 2 cut(s) 333, 689
BssECI CCNNGG 1 cut(s) 500
BssMI GATC 2 cut(s) 445, 518
BssT1I CCWWGG 1 cut(s) 500
Bst4CI ACNGT 4 cut(s) 129, 166, 665, 693
Bst6I CTCTTC 2 cut(s) 263, 280
BstAFI CTTAAG 1 cut(s) 628
BstAPI GCANNNNNTGC 2 cut(s) 149, 208
BstBAI YACGTR 2 cut(s) 260, 428
BstBI TTCGAA 1 cut(s) 108
BstC8I GCNNGC 4 cut(s) 198, 213, 372, 393
BstDEI CTNAG 2 cut(s) 458, 834
BstFNI CGCG 2 cut(s) 296, 727
BstHHI GCGC 2 cut(s) 100, 202
BstKTI GATC 2 cut(s) 448, 521
BstMBI GATC 2 cut(s) 445, 518
BstMWI GCNNNNNNNGC 4 cut(s) 97, 149, 208, 388
BstNSI RCATGY 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 507
BstUI CGCG 2 cut(s) 296, 727
BstV1I GCAGC 4 cut(s) 136, 469, 491, 811
BstV2I GAAGAC 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 469
BsuRI GGCC 3 cut(s) 5, 505, 744
BsuTUI ATCGAT 1 cut(s) 469
BtrI CACGTC 1 cut(s) 452
Cac8I GCNNGC 4 cut(s) 198, 213, 372, 393
CfoI GCGC 2 cut(s) 100, 202
Cfr13I GGNCC 2 cut(s) 643, 742
ClaI ATCGAT 1 cut(s) 469
CseI GACGC 1 cut(s) 302
CviAII CATG 2 cut(s) 197, 647
DdeI CTNAG 2 cut(s) 458, 834
DpnI GATC 2 cut(s) 447, 520
DpnII GATC 2 cut(s) 445, 518
DrdI GACNNNNNNGTC 1 cut(s) 790
DseDI GACNNNNNNGTC 1 cut(s) 790
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 263, 280
EarI CTCTTC 2 cut(s) 263, 280
Eco130I CCWWGG 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 500
FaeI CATG 2 cut(s) 200, 650
FaqI GGGAC 1 cut(s) 778
FatI CATG 2 cut(s) 196, 646
FauNDI CATATG 1 cut(s) 580
Fnu4HI GCNGC 5 cut(s) 150, 153, 480, 483, 825
Fsp4HI GCNGC 5 cut(s) 150, 153, 480, 483, 825
FspAI RTGCGCAY 1 cut(s) 201
FspI TGCGCA 1 cut(s) 201
GlaI GCGC 2 cut(s) 99, 201
GluI GCNGC 5 cut(s) 150, 153, 480, 483, 825
GsaI CCCAGC 1 cut(s) 219
HaeIII GGCC 3 cut(s) 5, 505, 744
HapII CCGG 3 cut(s) 398, 668, 821
HgaI GACGC 1 cut(s) 302
HhaI GCGC 2 cut(s) 100, 202
Hin1II CATG 2 cut(s) 200, 650
Hin6I GCGC 2 cut(s) 98, 200
HinP1I GCGC 2 cut(s) 98, 200
HinfI GANTC 8 cut(s) 36, 471, 554, 610, 616, 706, 784, 791
HpaII CCGG 3 cut(s) 398, 668, 821
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 4 cut(s) 67, 250, 292, 364
Hpy188I TCNGA 4 cut(s) 60, 549, 589, 615
Hpy188III TCNNGA 2 cut(s) 398, 788
Hpy8I GTNNAC 4 cut(s) 67, 250, 292, 364
HpyAV CCTTC 4 cut(s) 55, 55, 71, 347
HpyCH4III ACNGT 4 cut(s) 129, 166, 665, 693
HpyCH4IV ACGT 4 cut(s) 259, 323, 427, 451
HpyCH4V TGCA 4 cut(s) 196, 479, 485, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 149, 208, 388
HpyF3I CTNAG 2 cut(s) 458, 834
HpySE526I ACGT 4 cut(s) 259, 323, 427, 451
Hsp92II CATG 2 cut(s) 200, 650
HspAI GCGC 2 cut(s) 98, 200
Kpn2I TCCGGA 1 cut(s) 397
Kzo9I GATC 2 cut(s) 445, 518
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 4 cut(s) 136, 469, 491, 811
LweI GCATC 2 cut(s) 85, 341
MaeII ACGT 4 cut(s) 259, 323, 427, 451
MaeIII GTNAC 4 cut(s) 224, 324, 687, 703
MalI GATC 2 cut(s) 447, 520
MboI GATC 2 cut(s) 445, 518
MboII GAAGA 6 cut(s) 280, 297, 359, 362, 431, 446
MfeI CAATTG 1 cut(s) 711
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 17, 234, 491, 711, 731, 807
MluI ACGCGT 1 cut(s) 725
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 619, 700, 778, 800
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 3 cut(s) 63, 66, 116
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 397
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 177, 312, 339, 629, 753
MslI CAYNNNNRTG 1 cut(s) 565
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 482
MspCI CTTAAG 1 cut(s) 628
MspI CCGG 3 cut(s) 398, 668, 821
MunI CAATTG 1 cut(s) 711
MvnI CGCG 2 cut(s) 296, 727
MwoI GCNNNNNNNGC 4 cut(s) 97, 149, 208, 388
NdeI CATATG 1 cut(s) 580
NdeII GATC 2 cut(s) 445, 518
NlaIII CATG 2 cut(s) 200, 650
NmuCI GTSAC 1 cut(s) 703
NsbI TGCGCA 1 cut(s) 201
NspI RCATGY 1 cut(s) 200
NspV TTCGAA 1 cut(s) 108
PaeI GCATGC 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 36, 471, 554, 616
PkrI GCNGC 5 cut(s) 151, 154, 481, 484, 826
PleI GAGTC 4 cut(s) 618, 700, 778, 799
PpsI GAGTC 4 cut(s) 618, 700, 778, 799
Ppu21I YACGTR 2 cut(s) 260, 428
PspFI CCCAGC 1 cut(s) 215
PspPI GGNCC 2 cut(s) 643, 742
PvuII CAGCTG 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 565
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 5 cut(s) 177, 312, 339, 629, 753
SatI GCNGC 5 cut(s) 150, 153, 480, 483, 825
Sau3AI GATC 2 cut(s) 445, 518
Sau96I GGNCC 2 cut(s) 643, 742
SchI GAGTC 4 cut(s) 619, 700, 778, 800
SfaNI GCATC 2 cut(s) 85, 341
SfcI CTRYAG 1 cut(s) 507
SfuI TTCGAA 1 cut(s) 108
SinI GGWCC 1 cut(s) 643
SmiMI CAYNNNNRTG 1 cut(s) 565
SmlI CTYRAG 2 cut(s) 628, 786
SmoI CTYRAG 2 cut(s) 628, 786
SphI GCATGC 1 cut(s) 200
Sse9I AATT 6 cut(s) 17, 234, 491, 711, 731, 807
SsiI CCGC 1 cut(s) 152
StyI CCWWGG 1 cut(s) 500
TaaI ACNGT 4 cut(s) 129, 166, 665, 693
TaiI ACGT 4 cut(s) 262, 326, 430, 454
TaqI TCGA 3 cut(s) 108, 283, 469
TasI AATT 6 cut(s) 17, 234, 491, 711, 731, 807
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 4 cut(s) 36, 471, 554, 616
Tru1I TTAA 5 cut(s) 177, 312, 339, 629, 753
Tru9I TTAA 5 cut(s) 177, 312, 339, 629, 753
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 4 cut(s) 149, 479, 482, 824
Tsp45I GTSAC 1 cut(s) 703
Vha464I CTTAAG 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 643
XapI RAATTY 2 cut(s) 491, 731
XceI RCATGY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.