AT1G30080

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002223
Physical Location & Seq
Reverse (-)
10550855 .. 10553295
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30080.2

Sequence Viewer

Length: 1227 bp
ATGTCTAACATGTTTAGCAGAATTGCCATGACCAACTCTATCGTTCTTCTTCTATTTTCCTTGACTTTCTTAGAACATGGATTGTTGTTTCAAAGGGTCTCCTCTCTTGGCATCAACTATGGTCAAGTCGGCGACAATCTTCCTCCACCGGACAAAGTTCTACAACTCTTGAGTTCACTCCATATCAATAAGACAAGAATCTATGACACAAACCCTCGAGTGTTAACCTCATTTGCCAATTCCAATATCGAGCTCTTTGTCACCGTCGAAAATGAAATGCTTCCCAGCTTAGTAGACCCTCAACAAGCTCTCCAATGGGTCACTACCCGTATCAAACCCTACTTTCCGGCCACCAAGATCGGTGGTATCGCCGTCGGCAACGAACTATACACTGACGATGACTCAAGCCTTATAGGGTATCTTATGCCTGCAATGATGAGCATTCATGGGGCTTTGGTCCAAACCGGACTAGACAAGTACATTCAAGTATCGACTCCGAACTCACTATCCGTCCTTCAAGAATCTTACCCTCCATCCGCCGGATGTTTTAGGCCGGAAGTAGCTGGCGTAATGACACAGCTACTAGGTTTCTTGCGTAACACGAATTCCCCCTTCTGGATCAACGCTTACCCTTACTTCGCTTACAAGGATTCCCCAACAAAGATCCCATTAGACTACGTCCTCTTCAACCCTAACCCTGGAATGGTTGACCCCTATACCAAGTACCGCTATGACAACATGCTCTACGCTCAAGTAGACGCTGTGATCTTTGCCATGGCGAGGCTTGGCTTCAAAGACATCGAAGTTGGGGTCTCGGAGACTGGATGGCCGTCTAAAGGCGATGGAGATGAGGTTGGAGCCACTGTGGCCAACGCAGCCGTTTATAACAAAAATATTCTAAGGAGACAACTTCAAAATGAAGGAACGCCATTGAGACCAAACTTGAGTTTTGATGTTTATCTATTTGCTCTTTTCAATGAAGACCTTAAACCAGGACCAACCTCTGAGAGGAATTATGGATTGTATCAGCCTGATGAGACCATGACTTACAATGTAGGTTTATTATCGTCGTCATCATTAACATCAACATCAACTACTTCTTCAACTTCTATTATTTCCCTCACTTCCTCTGCCTCCACGGCTTTAAAGAAGGGAAAGCAACGGTTGATGTATTGGACATGCGTTTATTTGTTGGCCATTCACATGCTAATTAGAAGATCGTACTAG

Protein Analysis

408

Amino Acids

45.41

Weight (kDa)

6.12

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 36 - 352 1e-78 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 885
AccI GTMKAC 2 cut(s) 294, 756
AciI CCGC 2 cut(s) 537, 727
AclWI GGATC 2 cut(s) 626, 658
AcoI YGGCCR 4 cut(s) 348, 827, 867, 1194
AcsI RAATTY 1 cut(s) 604
AfaI GTAC 3 cut(s) 479, 725, 1223
AfiI CCNNNNNNNGG 7 cut(s) 539, 615, 698, 703, 780, 836, 1008
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 8 cut(s) 92, 485, 518, 688, 793, 914, 976, 1104
AjnI CCWGG 2 cut(s) 697, 991
AloI GAACNNNNNNTCC 2 cut(s) 491, 523
AluBI AGCT 5 cut(s) 253, 288, 308, 563, 580
AluI AGCT 5 cut(s) 253, 288, 308, 563, 580
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 6 cut(s) 103, 812, 817, 898, 928, 1031
AlwI GGATC 2 cut(s) 626, 658
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 5 cut(s) 348, 551, 827, 867, 1194
ApeKI GCWGC 1 cut(s) 875
ApoI RAATTY 1 cut(s) 604
ArsI GACNNNNNNTTYG 2 cut(s) 795, 827
Asp700I GAANNNNTTC 1 cut(s) 279
AspS9I GGNCC 2 cut(s) 457, 995
AsuHPI GGTGA 1 cut(s) 253
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 2 cut(s) 457, 995
BalI TGGCCA 2 cut(s) 869, 1196
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 987
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 887
BccI CCATC 3 cut(s) 541, 819, 836
BceAI ACGGC 4 cut(s) 356, 814, 863, 1155
BciT130I CCWGG 2 cut(s) 699, 993
BcoDI GTCTC 6 cut(s) 103, 812, 817, 898, 928, 1031
BfaI CTAG 3 cut(s) 470, 584, 1225
BglI GCCNNNNNGGC 2 cut(s) 866, 1139
BisI GCNGC 1 cut(s) 876
BlsI GCNGC 1 cut(s) 877
Bme1390I CCNGG 2 cut(s) 699, 993
Bme18I GGWCC 2 cut(s) 457, 995
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 2 cut(s) 457, 995
BmiI GGNNCC 1 cut(s) 859
BmrFI CCNGG 2 cut(s) 699, 993
BmsI GCATC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 987
BpuEI CTTGAG 4 cut(s) 190, 388, 735, 964
BsaBI GATNNNNATC 1 cut(s) 957
BsaI GGTCTC 4 cut(s) 103, 817, 928, 1031
BsaJI CCNNGG 3 cut(s) 697, 774, 1137
BsaWI WCCGGW 2 cut(s) 148, 464
BsaXI ACNNNNNCTCC 6 cut(s) 294, 324, 837, 849, 867, 879
Bsc4I CCNNNNNNNGG 7 cut(s) 539, 615, 698, 703, 780, 836, 1008
Bse1I ACTGG 1 cut(s) 826
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 957
BseBI CCWGG 2 cut(s) 699, 993
BseDI CCNNGG 3 cut(s) 697, 774, 1137
BseGI GGATG 3 cut(s) 533, 548, 830
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 7 cut(s) 539, 615, 698, 703, 780, 836, 1008
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 996
BseNI ACTGG 1 cut(s) 826
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 1 cut(s) 887
BseYI CCCAGC 1 cut(s) 284
BshFI GGCC 5 cut(s) 350, 553, 829, 869, 1196
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 5 cut(s) 149, 347, 465, 540, 554
BslI CCNNNNNNNGG 7 cut(s) 539, 615, 698, 703, 780, 836, 1008
BsmAI GTCTC 6 cut(s) 103, 812, 817, 898, 928, 1031
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 5 cut(s) 350, 553, 829, 869, 1196
Bso31I GGTCTC 4 cut(s) 103, 817, 928, 1031
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 5 cut(s) 357, 618, 663, 765, 1217
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 2 cut(s) 537, 727
BspANI GGCC 5 cut(s) 350, 553, 829, 869, 1196
BspCNI CTCAG 1 cut(s) 997
BspLI GGNNCC 1 cut(s) 859
BspPI GGATC 2 cut(s) 626, 658
BspTNI GGTCTC 4 cut(s) 103, 817, 928, 1031
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 1 cut(s) 826
BssECI CCNNGG 3 cut(s) 697, 774, 1137
BssMI GATC 5 cut(s) 357, 618, 663, 765, 1217
BssT1I CCWWGG 1 cut(s) 774
Bst2UI CCWGG 2 cut(s) 699, 993
Bst4CI ACNGT 3 cut(s) 265, 865, 1164
Bst6I CTCTTC 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 429, 565
BstDEI CTNAG 4 cut(s) 70, 289, 899, 1005
BstDSI CCRYGG 2 cut(s) 774, 1137
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 3 cut(s) 533, 548, 830
BstKTI GATC 5 cut(s) 360, 621, 666, 768, 1220
BstMAI GTCTC 6 cut(s) 103, 812, 817, 898, 928, 1031
BstMBI GATC 5 cut(s) 357, 618, 663, 765, 1217
BstMWI GCNNNNNNNGC 3 cut(s) 866, 875, 1139
BstNI CCWGG 2 cut(s) 699, 993
BstNSI RCATGY 4 cut(s) 13, 742, 1182, 1207
BstSCI CCNGG 2 cut(s) 697, 991
BstV1I GCAGC 1 cut(s) 887
BstV2I GAAGAC 1 cut(s) 987
BstX2I RGATCY 1 cut(s) 663
BstYI RGATCY 1 cut(s) 663
BsuRI GGCC 5 cut(s) 350, 553, 829, 869, 1196
BtgI CCRYGG 2 cut(s) 774, 1137
BtgZI GCGATG 1 cut(s) 855
BtsCI GGATG 3 cut(s) 533, 548, 830
BtsIMutI CAGTG 2 cut(s) 390, 861
Cac8I GCNNGC 2 cut(s) 429, 565
Cfr13I GGNCC 2 cut(s) 457, 995
CseI GACGC 1 cut(s) 767
Csp6I GTAC 3 cut(s) 478, 724, 1222
CspCI CAANNNNNGTGG 2 cut(s) 343, 378
CviAII CATG 9 cut(s) 10, 28, 77, 446, 739, 775, 1042, 1179, 1204
CviQI GTAC 3 cut(s) 478, 724, 1222
DdeI CTNAG 4 cut(s) 70, 289, 899, 1005
DpnI GATC 5 cut(s) 359, 620, 665, 767, 1219
DpnII GATC 5 cut(s) 357, 618, 663, 765, 1217
DraI TTTAAA 1 cut(s) 1146
EaeI YGGCCR 4 cut(s) 348, 827, 867, 1194
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
EciI GGCGGA 1 cut(s) 526
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 255
Eco31I GGTCTC 4 cut(s) 103, 817, 928, 1031
Eco47I GGWCC 2 cut(s) 457, 995
Eco53kI GAGCTC 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoRI GAATTC 1 cut(s) 604
EcoRII CCWGG 2 cut(s) 697, 991
EcoT14I CCWWGG 1 cut(s) 774
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 9 cut(s) 13, 31, 80, 449, 742, 778, 1045, 1182, 1207
FatI CATG 9 cut(s) 9, 27, 76, 445, 738, 774, 1041, 1178, 1203
FblI GTMKAC 2 cut(s) 294, 756
Fnu4HI GCNGC 1 cut(s) 876
FokI GGATG 3 cut(s) 520, 555, 837
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 876
FspBI CTAG 3 cut(s) 470, 584, 1225
GluI GCNGC 1 cut(s) 876
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 5 cut(s) 350, 553, 829, 869, 1196
HapII CCGG 5 cut(s) 149, 347, 465, 540, 554
HgaI GACGC 1 cut(s) 767
Hin1II CATG 9 cut(s) 13, 31, 80, 449, 742, 778, 1045, 1182, 1207
HincII GTYRAC 2 cut(s) 225, 709
HindII GTYRAC 2 cut(s) 225, 709
HinfI GANTC 5 cut(s) 198, 401, 493, 521, 650
HpaI GTTAAC 1 cut(s) 225
HpaII CCGG 5 cut(s) 149, 347, 465, 540, 554
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 5 cut(s) 176, 225, 295, 709, 757
Hpy188I TCNGA 3 cut(s) 498, 817, 1006
Hpy188III TCNNGA 3 cut(s) 169, 518, 616
Hpy8I GTNNAC 5 cut(s) 176, 225, 295, 709, 757
Hpy99I CGWCG 3 cut(s) 269, 377, 1072
HpyAV CCTTC 4 cut(s) 524, 622, 914, 1144
HpyCH4III ACNGT 3 cut(s) 265, 865, 1164
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 1 cut(s) 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 866, 875, 1139
HpyF3I CTNAG 4 cut(s) 70, 289, 899, 1005
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 9 cut(s) 13, 31, 80, 449, 742, 778, 1045, 1182, 1207
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 5 cut(s) 357, 618, 663, 765, 1217
LmnI GCTCC 1 cut(s) 857
Lsp1109I GCAGC 1 cut(s) 887
LweI GCATC 1 cut(s) 120
MaeI CTAG 3 cut(s) 470, 584, 1225
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 3 cut(s) 259, 319, 596
MalI GATC 5 cut(s) 359, 620, 665, 767, 1219
MboI GATC 5 cut(s) 357, 618, 663, 765, 1217
MboII GAAGA 7 cut(s) 38, 41, 131, 676, 992, 1092, 1227
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 2 cut(s) 869, 1196
MluCI AATT 5 cut(s) 21, 238, 604, 1012, 1209
MluNI TGGCCA 2 cut(s) 869, 1196
MlyI GAGTC 2 cut(s) 395, 487
MmeI TCCRAC 1 cut(s) 835
Mox20I TGGCCA 2 cut(s) 869, 1196
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 2 cut(s) 869, 1196
MseI TTAA 4 cut(s) 224, 987, 1079, 1145
MslI CAYNNNNRTG 1 cut(s) 1202
Msp20I TGGCCA 2 cut(s) 869, 1196
MspI CCGG 5 cut(s) 149, 347, 465, 540, 554
MspR9I CCNGG 2 cut(s) 699, 993
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 2 cut(s) 699, 993
MwoI GCNNNNNNNGC 3 cut(s) 866, 875, 1139
NcoI CCATGG 1 cut(s) 774
NdeII GATC 5 cut(s) 357, 618, 663, 765, 1217
NlaIII CATG 9 cut(s) 13, 31, 80, 449, 742, 778, 1045, 1182, 1207
NlaIV GGNNCC 1 cut(s) 859
NmuCI GTSAC 2 cut(s) 259, 319
NspI RCATGY 4 cut(s) 13, 742, 1182, 1207
PaeR7I CTCGAG 1 cut(s) 216
PciI ACATGT 1 cut(s) 9
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 198, 521, 650
PflFI GACNNNGTC 1 cut(s) 677
PkrI GCNGC 1 cut(s) 877
PleI GAGTC 2 cut(s) 395, 487
PpsI GAGTC 2 cut(s) 395, 487
PscI ACATGT 1 cut(s) 9
PsiI TTATAA 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 697, 991
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 2 cut(s) 697, 991
PspN4I GGNNCC 1 cut(s) 859
PspPI GGNCC 2 cut(s) 457, 995
PspXI VCTCGAGB 1 cut(s) 216
PsuI RGATCY 1 cut(s) 663
PsyI GACNNNGTC 1 cut(s) 677
RsaI GTAC 3 cut(s) 479, 725, 1223
RsaNI GTAC 3 cut(s) 478, 724, 1222
RseI CAYNNNNRTG 1 cut(s) 1202
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 4 cut(s) 224, 987, 1079, 1145
SatI GCNGC 1 cut(s) 876
Sau3AI GATC 5 cut(s) 357, 618, 663, 765, 1217
Sau96I GGNCC 2 cut(s) 457, 995
SchI GAGTC 2 cut(s) 395, 487
ScrFI CCNGG 2 cut(s) 699, 993
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 1 cut(s) 120
Sfr274I CTCGAG 1 cut(s) 216
SinI GGWCC 2 cut(s) 457, 995
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 1202
SmlI CTYRAG 5 cut(s) 169, 216, 403, 750, 943
SmoI CTYRAG 5 cut(s) 169, 216, 403, 750, 943
Sse9I AATT 5 cut(s) 21, 238, 604, 1012, 1209
SsiI CCGC 2 cut(s) 537, 727
SspI AATATT 1 cut(s) 895
SspMI CTAG 3 cut(s) 470, 584, 1225
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 697, 991
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 3 cut(s) 265, 865, 1164
TaiI ACGT 1 cut(s) 681
TaqI TCGA 5 cut(s) 217, 249, 267, 491, 801
TasI AATT 5 cut(s) 21, 238, 604, 1012, 1209
TatI WGTACW 1 cut(s) 477
TfiI GAWTC 3 cut(s) 198, 521, 650
Tru1I TTAA 4 cut(s) 224, 987, 1079, 1145
Tru9I TTAA 4 cut(s) 224, 987, 1079, 1145
TscAI CASTG 2 cut(s) 397, 868
TseFI GTSAC 2 cut(s) 259, 319
TseI GCWGC 1 cut(s) 875
Tsp45I GTSAC 2 cut(s) 259, 319
TspDTI ATGAA 4 cut(s) 288, 434, 933, 993
TspGWI ACGGA 1 cut(s) 499
TspRI CASTG 2 cut(s) 397, 868
Tth111I GACNNNGTC 1 cut(s) 677
VpaK11BI GGWCC 2 cut(s) 457, 995
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 1 cut(s) 604
XceI RCATGY 4 cut(s) 13, 742, 1182, 1207
XhoI CTCGAG 1 cut(s) 216
XmiI GTMKAC 2 cut(s) 294, 756
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 3 cut(s) 470, 584, 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.