pycom10g21790

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002223
Physical Location & Seq
Forward (+)
24364459 .. 24365271
813 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21790.1

Sequence Viewer

Length: 813 bp
ATGATGACACAACAGTACTCTTATCCAATCTTGTCCCGGCCATCGATCAGCATCCAGACGGCACTAGCTAAGCTAGGCCTTGATTCCTACATTAAAATCTCCACCCCTTGTTCTCTGGCGGTCCTCAAAGAATCCTACCCGCCTTCAGCAGGGAGTTTCAAACCCGAGGTGGCTCAGGTCATGACACAACTGTTACAGTTCTTGCAAAGCACAAAGGCACCTTTTTGGATCAATGCCTATCCATATTTTTCTTACAAGGGTGATCCAAACAGAGTCTCTTTGGACTATGTACTATTTAACGCCAACCAAGGGATGGTCGACCCTTTCACCAAGCTACACTATGACAACATGTTGTATGCACAAGTAGATGCCGCCATTTATGCCATGGCTAGGTTAGGGTTCAATGGTATTGAGGTTAAGGTTTCGGAAACGGGCTGGCCATCCAGGGGGGACTCAAACGAAATCGGTGCAACGCTACAGAATGCTGCCACTTACAACAGGAATCTGTTGAGAAGGCAGCTCCAGAATGAAGGCACTCCTCTGAGGCCTAAATTGAGGCTGGAGGTCTACGTGTTTGCTTTGTTCAATGAGGATATGAAGCCCGGACCAACGTCAGAAAGGAACTACGGACTATTTCAGCCGGATGGGACTATGGCTTACAATGTTGGACTGTCTGCCATATCAACTAGTAGAACACCAACTTCATCATCAACAACATCAGCTTCCATTTCTGATGTCTTTTCCTCTGCTACAAAGGTAAAAAAACCACAGGCTCTTTCTCACTTTCCTCAGAATGATTATTTTTTTCCTTAA

Protein Analysis

271

Amino Acids

30.05

Weight (kDa)

8.98

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 17 - 222 5.8e-54 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 2 cut(s) 318, 567
AciI CCGC 3 cut(s) 119, 140, 372
AclWI GGATC 2 cut(s) 236, 257
AcoI YGGCCR 2 cut(s) 38, 437
AcuI CTGAAG 1 cut(s) 129
AfaI GTAC 2 cut(s) 17, 291
AfiI CCNNNNNNNGG 5 cut(s) 149, 309, 313, 390, 446
AflIII ACRYGT 2 cut(s) 348, 570
AgsI TTSAA 3 cut(s) 160, 403, 586
AhlI ACTAGT 1 cut(s) 686
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 5 cut(s) 68, 73, 334, 520, 722
AluI AGCT 5 cut(s) 68, 73, 334, 520, 722
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 236, 257
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 4 cut(s) 38, 76, 437, 545
ApeKI GCWGC 2 cut(s) 485, 517
AspS9I GGNCC 2 cut(s) 121, 605
AsuC2I CCSGG 2 cut(s) 37, 603
AsuHPI GGTGA 2 cut(s) 272, 319
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 2 cut(s) 121, 605
BalI TGGCCA 1 cut(s) 439
BanI GGYRCC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 472, 529
BccI CCATC 4 cut(s) 49, 307, 448, 638
BceAI ACGGC 1 cut(s) 75
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 1 cut(s) 445
BcnI CCSGG 2 cut(s) 37, 603
BcoDI GTCTC 1 cut(s) 280
BcuI ACTAGT 1 cut(s) 686
BfaI CTAG 4 cut(s) 65, 74, 390, 687
BfmI CTRYAG 1 cut(s) 476
BisI GCNGC 3 cut(s) 372, 486, 518
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 3 cut(s) 373, 487, 519
BmcAI AGTACT 1 cut(s) 17
Bme1390I CCNGG 3 cut(s) 37, 445, 603
Bme18I GGWCC 2 cut(s) 121, 605
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 121, 605
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 3 cut(s) 37, 445, 603
BmsI GCATC 2 cut(s) 60, 358
BoxI GACNNNNGTC 1 cut(s) 610
BpmI CTGGAG 2 cut(s) 506, 581
Bpu10I CCTNAGC 1 cut(s) 174
Bpu1102I GCTNAGC 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 37, 603
Bsa29I ATCGAT 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 571
BsaBI GATNNNNATC 1 cut(s) 50
BsaJI CCNNGG 4 cut(s) 165, 307, 384, 444
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 309, 313, 390, 446
Bse8I GATNNNNATC 1 cut(s) 50
BseBI CCWGG 1 cut(s) 445
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 4 cut(s) 165, 307, 384, 444
BseGI GGATG 4 cut(s) 51, 318, 440, 649
BseJI GATNNNNATC 1 cut(s) 50
BseLI CCNNNNNNNGG 5 cut(s) 149, 309, 313, 390, 446
BseMII CTCAG 3 cut(s) 188, 533, 803
BseRI GAGGAG 1 cut(s) 528
BseXI GCAGC 2 cut(s) 472, 529
BshFI GGCC 4 cut(s) 40, 78, 439, 547
BshNI GGYRCC 1 cut(s) 217
BshVI ATCGAT 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 3 cut(s) 37, 603, 641
BslFI GGGAC 3 cut(s) 19, 464, 661
BslI CCNNNNNNNGG 5 cut(s) 149, 309, 313, 390, 446
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 3 cut(s) 19, 464, 661
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 4 cut(s) 40, 78, 439, 547
BsoBI CYCGRG 1 cut(s) 164
Bsp143I GATC 3 cut(s) 45, 228, 262
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 119, 140, 372
BspANI GGCC 4 cut(s) 40, 78, 439, 547
BspCNI CTCAG 3 cut(s) 187, 534, 802
BspDI ATCGAT 1 cut(s) 44
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 2 cut(s) 236, 257
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 4 cut(s) 165, 307, 384, 444
BssMI GATC 3 cut(s) 45, 228, 262
BssT1I CCWWGG 2 cut(s) 307, 384
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 4 cut(s) 15, 192, 198, 672
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 437
BstDEI CTNAG 4 cut(s) 69, 174, 542, 789
BstDSI CCRYGG 1 cut(s) 384
BstENI CCTNNNNNAGG 1 cut(s) 147
BstF5I GGATG 4 cut(s) 51, 318, 440, 649
BstKTI GATC 3 cut(s) 48, 231, 265
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 45, 228, 262
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 1 cut(s) 352
BstPAI GACNNNNGTC 1 cut(s) 610
BstSCI CCNGG 3 cut(s) 35, 443, 601
BstSFI CTRYAG 1 cut(s) 476
BstV1I GCAGC 2 cut(s) 472, 529
Bsu15I ATCGAT 1 cut(s) 44
BsuRI GGCC 4 cut(s) 40, 78, 439, 547
BsuTUI ATCGAT 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 4 cut(s) 51, 318, 440, 649
Cac8I GCNNGC 1 cut(s) 437
CciI TCATGA 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 121, 605
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 2 cut(s) 16, 290
CviAII CATG 3 cut(s) 181, 349, 385
CviQI GTAC 2 cut(s) 16, 290
DdeI CTNAG 4 cut(s) 69, 174, 542, 789
DpnI GATC 3 cut(s) 47, 230, 264
DpnII GATC 3 cut(s) 45, 228, 262
EaeI YGGCCR 2 cut(s) 38, 437
Eco130I CCWWGG 2 cut(s) 307, 384
Eco147I AGGCCT 2 cut(s) 78, 547
Eco47I GGWCC 2 cut(s) 121, 605
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 443
EcoT14I CCWWGG 2 cut(s) 307, 384
ErhI CCWWGG 2 cut(s) 307, 384
FaeI CATG 3 cut(s) 184, 352, 388
FaqI GGGAC 3 cut(s) 19, 464, 661
FatI CATG 3 cut(s) 180, 348, 384
FauI CCCGC 1 cut(s) 147
FblI GTMKAC 2 cut(s) 318, 567
Fnu4HI GCNGC 3 cut(s) 372, 486, 518
FokI GGATG 4 cut(s) 38, 325, 427, 656
Fsp4HI GCNGC 3 cut(s) 372, 486, 518
FspBI CTAG 4 cut(s) 65, 74, 390, 687
GluI GCNGC 3 cut(s) 372, 486, 518
GsuI CTGGAG 2 cut(s) 506, 581
HaeIII GGCC 4 cut(s) 40, 78, 439, 547
HapII CCGG 3 cut(s) 37, 603, 641
Hin1II CATG 3 cut(s) 184, 352, 388
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 5 cut(s) 83, 131, 273, 452, 502
HpaII CCGG 3 cut(s) 37, 603, 641
HphI GGTGA 2 cut(s) 272, 319
Hpy166II GTNNAC 2 cut(s) 319, 568
Hpy188I TCNGA 5 cut(s) 427, 543, 616, 733, 792
Hpy188III TCNNGA 3 cut(s) 55, 181, 523
Hpy8I GTNNAC 2 cut(s) 319, 568
HpyAV CCTTC 3 cut(s) 153, 507, 524
HpyCH4III ACNGT 4 cut(s) 15, 192, 198, 672
HpyCH4IV ACGT 2 cut(s) 570, 611
HpyCH4V TGCA 3 cut(s) 205, 359, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 4 cut(s) 69, 174, 542, 789
HpySE526I ACGT 2 cut(s) 570, 611
Hsp92II CATG 3 cut(s) 184, 352, 388
Kzo9I GATC 3 cut(s) 45, 228, 262
LmnI GCTCC 1 cut(s) 525
Lsp1109I GCAGC 2 cut(s) 472, 529
LweI GCATC 2 cut(s) 60, 358
MaeI CTAG 4 cut(s) 65, 74, 390, 687
MaeII ACGT 2 cut(s) 570, 611
MaeIII GTNAC 1 cut(s) 192
MalI GATC 3 cut(s) 47, 230, 264
MboI GATC 3 cut(s) 45, 228, 262
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 1 cut(s) 551
MluNI TGGCCA 1 cut(s) 439
MlyI GAGTC 2 cut(s) 282, 446
MmeI TCCRAC 1 cut(s) 646
Mox20I TGGCCA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 439
MseI TTAA 4 cut(s) 93, 297, 417, 811
Msp20I TGGCCA 1 cut(s) 439
MspI CCGG 3 cut(s) 37, 603, 641
MspR9I CCNGG 3 cut(s) 37, 445, 603
Mva1269I GAATGC 1 cut(s) 487
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 1 cut(s) 380
NciI CCSGG 2 cut(s) 37, 603
NcoI CCATGG 1 cut(s) 384
NdeII GATC 3 cut(s) 45, 228, 262
NlaIII CATG 3 cut(s) 184, 352, 388
NlaIV GGNNCC 1 cut(s) 219
NspI RCATGY 1 cut(s) 352
PagI TCATGA 1 cut(s) 180
PceI AGGCCT 2 cut(s) 78, 547
PciI ACATGT 1 cut(s) 348
PctI GAATGC 1 cut(s) 487
PfeI GAWTC 3 cut(s) 83, 131, 502
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 3 cut(s) 373, 487, 519
PleI GAGTC 2 cut(s) 281, 446
PpsI GAGTC 2 cut(s) 281, 446
Ppu21I YACGTR 1 cut(s) 571
PscI ACATGT 1 cut(s) 348
PshAI GACNNNNGTC 1 cut(s) 610
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 2 cut(s) 121, 605
RsaI GTAC 2 cut(s) 17, 291
RsaNI GTAC 2 cut(s) 16, 290
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 4 cut(s) 93, 297, 417, 811
SatI GCNGC 3 cut(s) 372, 486, 518
Sau3AI GATC 3 cut(s) 45, 228, 262
Sau96I GGNCC 2 cut(s) 121, 605
ScaI AGTACT 1 cut(s) 17
SchI GAGTC 2 cut(s) 282, 446
ScrFI CCNGG 3 cut(s) 37, 445, 603
SfaNI GCATC 2 cut(s) 60, 358
SfcI CTRYAG 1 cut(s) 476
SinI GGWCC 2 cut(s) 121, 605
SpeI ACTAGT 1 cut(s) 686
Sse9I AATT 1 cut(s) 551
SseBI AGGCCT 2 cut(s) 78, 547
SsiI CCGC 3 cut(s) 119, 140, 372
SspMI CTAG 4 cut(s) 65, 74, 390, 687
StuI AGGCCT 2 cut(s) 78, 547
StyD4I CCNGG 3 cut(s) 35, 443, 601
StyI CCWWGG 2 cut(s) 307, 384
TaaI ACNGT 4 cut(s) 15, 192, 198, 672
TaiI ACGT 2 cut(s) 573, 614
TaqI TCGA 2 cut(s) 44, 318
TasI AATT 1 cut(s) 551
TatI WGTACW 2 cut(s) 15, 289
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 83, 131, 502
Tru1I TTAA 4 cut(s) 93, 297, 417, 811
Tru9I TTAA 4 cut(s) 93, 297, 417, 811
TseI GCWGC 2 cut(s) 485, 517
TspDTI ATGAA 3 cut(s) 543, 611, 693
TspGWI ACGGA 1 cut(s) 642
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 121, 605
XagI CCTNNNNNAGG 1 cut(s) 147
XceI RCATGY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 2 cut(s) 310, 382
XmiI GTMKAC 2 cut(s) 318, 567
XspI CTAG 4 cut(s) 65, 74, 390, 687
ZrmI AGTACT 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.