RchiOBHm_Chr5g0006351

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002223
Physical Location & Seq
Forward (+)
3953935 .. 3955022
1088 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28748

Sequence Viewer

Length: 387 bp
ATGCTAGCTGCATTTGCAAATTCCGGCAATGAGCTAACTGTAACAATTGAAAACGCAATGCTGAGCGAATTAACCAACCCCCAAGCAGCCCTTCTCTGGGTTAGCACACACATCAAGCCTTATTGTCCCGCAACAATCACCATCATTGCGGTAGGGAATGAGGTGGATGCAACCGACTTGCTAGCTAACCTTGTACCGGCCATGTCCAACATTGAAGGGGCACTATCTCAATTAGGCCTTGATTCATACATCAAGCCTGGACCAACTTCAGAGAGGAATTTTGGTGTCTATCAACCAAATGGAACTAGAGCTTACAATCTGGGCAACAATAGGAAGCCGGATGTACTTGATCATGTTTGTTGCTTTGTTGACTTTCCAAGGATATAA

Protein Analysis

128

Amino Acids

13.81

Weight (kDa)

4.74

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 2 - 85 6.2e-11 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 129, 149
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 2 cut(s) 19, 277
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 2 cut(s) 195, 345
AfiI CCNNNNNNNGG 3 cut(s) 96, 97, 196
AgsI TTSAA 2 cut(s) 50, 215
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 4 cut(s) 8, 34, 185, 311
AluI AGCT 4 cut(s) 8, 34, 185, 311
AoxI GGCC 2 cut(s) 198, 235
ApeKI GCWGC 2 cut(s) 8, 86
ApoI RAATTY 2 cut(s) 19, 277
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 130
AsuNHI GCTAGC 2 cut(s) 4, 181
AvaII GGWCC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 98
BccI CCATC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 258
BclI TGATCA 1 cut(s) 349
BfaI CTAG 3 cut(s) 5, 182, 306
BisI GCNGC 2 cut(s) 9, 87
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 2 cut(s) 10, 88
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 157
BmtI GCTAGC 2 cut(s) 8, 185
Bpu1102I GCTNAGC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 377
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 97, 196
Bse118I RCCGGY 1 cut(s) 196
Bse3DI GCAATG 3 cut(s) 34, 63, 144
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 2 cut(s) 172, 346
BseLI CCNNNNNNNGG 3 cut(s) 96, 97, 196
BseMI GCAATG 3 cut(s) 34, 63, 144
BseMII CTCAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 1 cut(s) 98
BshFI GGCC 2 cut(s) 200, 237
BsiSI CCGG 3 cut(s) 24, 197, 338
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 3 cut(s) 96, 97, 196
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 2 cut(s) 200, 237
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 349
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 2 cut(s) 129, 149
BspANI GGCC 2 cut(s) 200, 237
BspCNI CTCAG 1 cut(s) 54
BspOI GCTAGC 2 cut(s) 8, 185
BsrDI GCAATG 3 cut(s) 34, 63, 144
BsrFI RCCGGY 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 6, 183
BstDEI CTNAG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 172, 346
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 1 cut(s) 98
BsuRI GGCC 2 cut(s) 200, 237
BtsCI GGATG 2 cut(s) 172, 346
Cac8I GCNNGC 2 cut(s) 6, 183
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 194, 344
CviAII CATG 2 cut(s) 202, 353
CviQI GTAC 2 cut(s) 194, 344
DdeI CTNAG 1 cut(s) 62
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 377
Eco147I AGGCCT 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 2 cut(s) 205, 356
FaiI YATR 4 cut(s) 203, 247, 354, 385
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 111
FatI CATG 2 cut(s) 201, 352
FauI CCCGC 1 cut(s) 136
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 9, 87
FokI GGATG 2 cut(s) 179, 353
Fsp4HI GCNGC 2 cut(s) 9, 87
FspBI CTAG 3 cut(s) 5, 182, 306
GluI GCNGC 2 cut(s) 9, 87
HaeIII GGCC 2 cut(s) 200, 237
HapII CCGG 3 cut(s) 24, 197, 338
Hin1II CATG 2 cut(s) 205, 356
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 1 cut(s) 242
HpaII CCGG 3 cut(s) 24, 197, 338
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 370
HpyAV CCTTC 2 cut(s) 101, 209
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 3 cut(s) 11, 17, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 1 cut(s) 62
Hsp92II CATG 2 cut(s) 205, 356
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 1 cut(s) 349
LpnPI CCDG 7 cut(s) 37, 82, 210, 243, 270, 305, 351
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 1 cut(s) 157
MaeI CTAG 3 cut(s) 5, 182, 306
MaeIII GTNAC 1 cut(s) 40
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MfeI CAATTG 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 5 cut(s) 19, 45, 68, 230, 277
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 2 cut(s) 154, 267
MseI TTAA 1 cut(s) 71
MspI CCGG 3 cut(s) 24, 197, 338
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 1 cut(s) 349
NheI GCTAGC 2 cut(s) 4, 181
NlaIII CATG 2 cut(s) 205, 356
PceI AGGCCT 1 cut(s) 237
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 10, 88
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspPI GGNCC 1 cut(s) 260
RsaI GTAC 2 cut(s) 195, 345
RsaNI GTAC 2 cut(s) 194, 344
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 2 cut(s) 9, 87
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 223
SetI ASST 6 cut(s) 10, 36, 165, 187, 192, 313
SfaNI GCATC 1 cut(s) 157
SinI GGWCC 1 cut(s) 260
Sse9I AATT 5 cut(s) 19, 45, 68, 230, 277
SseBI AGGCCT 1 cut(s) 237
SsiI CCGC 2 cut(s) 129, 149
SspMI CTAG 3 cut(s) 5, 182, 306
StuI AGGCCT 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 1 cut(s) 40
TasI AATT 5 cut(s) 19, 45, 68, 230, 277
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TseI GCWGC 2 cut(s) 8, 86
TspDTI ATGAA 1 cut(s) 234
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 2 cut(s) 19, 277
XspI CTAG 3 cut(s) 5, 182, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.