Rh4CG383200

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002223
Physical Location & Seq
Forward (+)
63639535 .. 63640481
947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG383200.1

Sequence Viewer

Length: 915 bp
ATGTCCAGCATTCAAGGGGCACTTTCTCAGCTAGGCCTTGACTCACACATCAAGGTGTCATCGCCTTTTTCTTATTCGGTGATCAAAGAGTCTTATCCGCCTTCTGCTGGCAGTTTCAAAAGCGAGGTCTCCAGTGTAATGTCACAATTGTTACAGTTTCTGTCAAGCACAAAGGCACCATTTTGGATCAATGCATATCCTTACTTTGCATACAAGGGCAACTCGAAAGAAATTCCTCTAGACTATGTGCTTTTCAGTAATGCCGGTTCATCAGCAGGAGTGACTGACCCTACTAGCAATTTGCACTATGACAACATGTTGTATGCTCAAGTAGATGCAGTCACTTATGCCATTTCTAAATTGGGCTTCGATGGCATAGAGGTTAGGGTCACAGAGACAGGGTGGCCATCAAAAGGGGATGCTGGTGAACTTGGTGCAAGTTTAGAGAATGCTGCTATATACAATGGGAACTTGTTGAAGAGGCAATTGGCCAACGAAGGTACACCTTTGAAGCCTAAGCTTGACGTCTATATTTTTGCTCTTTTCAACGAGAACAAGAAGCCTGGACCAACATCGGAGAGGAATTTTGGTCTATTTCAACCGGATGGAACTTCGGCTTACAATTTGGGCTTGTCTGGATCATCATCTACCTCAACTAATGCCCCATCACCCCCATCGCCAAGCCTATCAAAACCACCAACTTCAGCTACCAACTCAACTGGTGCCTCATCATCACCCTCAATCCCAATAAAATCATCAAGCTCGGCTACTAATTCAACTAATGCCTCATCCCCCTCGTCACTTCCTGAGATTGATATGACTTATTCCTCTAGCAGTGCCAAGGCAGCAACAATGGGAAGCTTGGTGTACATTTTTCTTGGTGTATTGACCTTCCAATTTTATATGATGATCTAG

Protein Analysis

304

Amino Acids

32.14

Weight (kDa)

5.73

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 1 - 209 1.3e-52 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 528
AccB1I GGYRCC 2 cut(s) 175, 722
AciI CCGC 1 cut(s) 98
AclWI GGATC 2 cut(s) 194, 646
AcoI YGGCCR 2 cut(s) 404, 489
AcsI RAATTY 2 cut(s) 231, 583
AcuI CTGAAG 1 cut(s) 687
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 2 cut(s) 502, 869
AfiI CCNNNNNNNGG 2 cut(s) 107, 413
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 7 cut(s) 14, 118, 478, 511, 547, 599, 777
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 5 cut(s) 31, 520, 707, 762, 861
AluI AGCT 5 cut(s) 31, 520, 707, 762, 861
Alw26I GTCTC 2 cut(s) 133, 389
AlwI GGATC 2 cut(s) 194, 646
AlwNI CAGNNNCTG 1 cut(s) 160
AoxI GGCC 3 cut(s) 34, 404, 489
ApeKI GCWGC 2 cut(s) 452, 845
ApoI RAATTY 2 cut(s) 231, 583
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AspS9I GGNCC 1 cut(s) 566
AsuHPI GGTGA 4 cut(s) 91, 437, 660, 726
AvaII GGWCC 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 22
BalI TGGCCA 2 cut(s) 406, 491
BanI GGYRCC 2 cut(s) 175, 722
BbvI GCAGC 2 cut(s) 439, 857
BccI CCATC 5 cut(s) 365, 415, 599, 673, 682
BciT130I CCWGG 1 cut(s) 564
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 133, 389
BfaI CTAG 5 cut(s) 32, 239, 294, 831, 913
BisI GCNGC 2 cut(s) 453, 846
BlsI GCNGC 2 cut(s) 454, 847
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 177, 724
BmrFI CCNGG 1 cut(s) 564
BmsI GCATC 2 cut(s) 325, 409
BpmI CTGGAG 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 643
BsaHI GRCGYC 1 cut(s) 525
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 840
BsaWI WCCGGW 1 cut(s) 601
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 413
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 132, 724
Bse8I GATNNNNATC 1 cut(s) 643
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 1 cut(s) 840
BseGI GGATG 3 cut(s) 424, 610, 788
BseJI GATNNNNATC 1 cut(s) 643
BseLI CCNNNNNNNGG 2 cut(s) 107, 413
BseMII CTCAG 2 cut(s) 41, 798
BseNI ACTGG 2 cut(s) 132, 724
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 2 cut(s) 439, 857
BshFI GGCC 3 cut(s) 36, 406, 491
BshNI GGYRCC 2 cut(s) 175, 722
BsiSI CCGG 2 cut(s) 264, 602
BslI CCNNNNNNNGG 2 cut(s) 107, 413
BsmAI GTCTC 2 cut(s) 133, 389
BsmI GAATGC 2 cut(s) 9, 454
BsnI GGCC 3 cut(s) 36, 406, 491
Bso31I GGTCTC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 22
Bsp1407I TGTACA 1 cut(s) 867
Bsp143I GATC 4 cut(s) 81, 186, 638, 909
BspACI CCGC 1 cut(s) 98
BspANI GGCC 3 cut(s) 36, 406, 491
BspCNI CTCAG 2 cut(s) 40, 799
BspLI GGNNCC 2 cut(s) 177, 724
BspPI GGATC 2 cut(s) 194, 646
BspT107I GGYRCC 2 cut(s) 175, 722
BspTNI GGTCTC 1 cut(s) 133
BsrFI RCCGGY 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 867
BsrI ACTGG 2 cut(s) 132, 724
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 840
BssMI GATC 4 cut(s) 81, 186, 638, 909
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 840
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 1 cut(s) 156
Bst6I CTCTTC 1 cut(s) 473
BstACI GRCGYC 1 cut(s) 525
BstAUI TGTACA 1 cut(s) 867
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 3 cut(s) 27, 516, 807
BstF5I GGATG 3 cut(s) 424, 610, 788
BstKTI GATC 4 cut(s) 84, 189, 641, 912
BstMAI GTCTC 2 cut(s) 133, 389
BstMBI GATC 4 cut(s) 81, 186, 638, 909
BstMWI GCNNNNNNNGC 2 cut(s) 372, 845
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 562
BstSLI GKGCMC 1 cut(s) 22
BstV1I GCAGC 2 cut(s) 439, 857
BsuRI GGCC 3 cut(s) 36, 406, 491
BtgZI GCGATG 2 cut(s) 45, 660
BtsCI GGATG 3 cut(s) 424, 610, 788
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 2 cut(s) 139, 841
Cac8I GCNNGC 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 566
Csp6I GTAC 2 cut(s) 501, 868
CviAII CATG 1 cut(s) 316
CviQI GTAC 2 cut(s) 501, 868
DdeI CTNAG 3 cut(s) 27, 516, 807
DpnI GATC 4 cut(s) 83, 188, 640, 911
DpnII GATC 4 cut(s) 81, 186, 638, 909
EaeI YGGCCR 2 cut(s) 404, 489
Eam1104I CTCTTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 473
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 840
Eco147I AGGCCT 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 687
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 840
EcoT22I ATGCAT 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 1 cut(s) 319
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 1 cut(s) 315
FbaI TGATCA 1 cut(s) 81
Fnu4HI GCNGC 2 cut(s) 453, 846
FokI GGATG 3 cut(s) 431, 617, 775
Fsp4HI GCNGC 2 cut(s) 453, 846
FspBI CTAG 5 cut(s) 32, 239, 294, 831, 913
GluI GCNGC 2 cut(s) 453, 846
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 3 cut(s) 36, 406, 491
HapII CCGG 2 cut(s) 264, 602
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 1 cut(s) 319
HindIII AAGCTT 2 cut(s) 518, 859
HinfI GANTC 2 cut(s) 41, 89
HpaII CCGG 2 cut(s) 264, 602
HphI GGTGA 4 cut(s) 91, 437, 660, 726
Hpy166II GTNNAC 3 cut(s) 428, 503, 868
Hpy188I TCNGA 1 cut(s) 577
Hpy188III TCNNGA 3 cut(s) 239, 636, 806
Hpy8I GTNNAC 3 cut(s) 428, 503, 868
HpyAV CCTTC 3 cut(s) 111, 491, 901
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 5 cut(s) 194, 209, 304, 338, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 845
HpyF3I CTNAG 3 cut(s) 27, 516, 807
HpySE526I ACGT 1 cut(s) 525
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 4 cut(s) 81, 186, 638, 909
Lsp1109I GCAGC 2 cut(s) 439, 857
LweI GCATC 2 cut(s) 325, 409
MaeI CTAG 5 cut(s) 32, 239, 294, 831, 913
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 6 cut(s) 141, 150, 280, 340, 388, 798
MalI GATC 4 cut(s) 83, 188, 640, 911
MboI GATC 4 cut(s) 81, 186, 638, 909
MboII GAAGA 1 cut(s) 490
MfeI CAATTG 2 cut(s) 146, 485
MhlI GDGCHC 1 cut(s) 22
MlsI TGGCCA 2 cut(s) 406, 491
MluCI AATT 9 cut(s) 146, 231, 298, 359, 485, 583, 622, 772, 896
MluNI TGGCCA 2 cut(s) 406, 491
MlyI GAGTC 2 cut(s) 35, 98
Mox20I TGGCCA 2 cut(s) 406, 491
Mph1103I ATGCAT 1 cut(s) 196
MscI TGGCCA 2 cut(s) 406, 491
MslI CAYNNNNRTG 1 cut(s) 53
Msp20I TGGCCA 2 cut(s) 406, 491
MspI CCGG 2 cut(s) 264, 602
MspR9I CCNGG 1 cut(s) 564
MunI CAATTG 2 cut(s) 146, 485
Mva1269I GAATGC 2 cut(s) 9, 454
MvaI CCWGG 1 cut(s) 564
MwoI GCNNNNNNNGC 2 cut(s) 372, 845
NdeII GATC 4 cut(s) 81, 186, 638, 909
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 2 cut(s) 177, 724
NmeAIII GCCGAG 1 cut(s) 743
NmuCI GTSAC 5 cut(s) 141, 280, 340, 388, 798
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 319
PceI AGGCCT 1 cut(s) 36
PciI ACATGT 1 cut(s) 315
PctI GAATGC 2 cut(s) 9, 454
PkrI GCNGC 2 cut(s) 454, 847
PleI GAGTC 2 cut(s) 35, 97
PpsI GAGTC 2 cut(s) 35, 97
PscI ACATGT 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 2 cut(s) 177, 724
PspPI GGNCC 1 cut(s) 566
PsrI GAACNNNNNNTAC 2 cut(s) 250, 282
PstNI CAGNNNCTG 1 cut(s) 160
RsaI GTAC 2 cut(s) 502, 869
RsaNI GTAC 2 cut(s) 501, 868
RseI CAYNNNNRTG 1 cut(s) 53
SatI GCNGC 2 cut(s) 453, 846
Sau3AI GATC 4 cut(s) 81, 186, 638, 909
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 2 cut(s) 35, 98
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 22
SfaNI GCATC 2 cut(s) 325, 409
SinI GGWCC 1 cut(s) 566
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 9 cut(s) 146, 231, 298, 359, 485, 583, 622, 772, 896
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 1 cut(s) 98
SspMI CTAG 5 cut(s) 32, 239, 294, 831, 913
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 562
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 1 cut(s) 156
TaiI ACGT 1 cut(s) 528
TaqI TCGA 2 cut(s) 224, 369
TasI AATT 9 cut(s) 146, 231, 298, 359, 485, 583, 622, 772, 896
TatI WGTACW 1 cut(s) 867
TscAI CASTG 2 cut(s) 139, 841
TseFI GTSAC 5 cut(s) 141, 280, 340, 388, 798
TseI GCWGC 2 cut(s) 452, 845
Tsp45I GTSAC 5 cut(s) 141, 280, 340, 388, 798
TspDTI ATGAA 1 cut(s) 258
TspRI CASTG 2 cut(s) 139, 841
VpaK11BI GGWCC 1 cut(s) 566
XapI RAATTY 2 cut(s) 231, 583
XbaI TCTAGA 1 cut(s) 238
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XspI CTAG 5 cut(s) 32, 239, 294, 831, 913
ZraI GACGTC 1 cut(s) 526
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.