RLG00000032094

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002223
Physical Location & Seq
Reverse (-)
10846315 .. 10847734
1420 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032094

Sequence Viewer

Length: 1212 bp
ATGGAAATTTCTAGCAGAATTACCAGAATCAATGCTTTTCTTCTCTGTTTGCTTTGTATCTCAGAATTACAAGAAGTTACATCACTTGGCATCAACTATGGCCAACTCGGCAACAATTTGCTAGAACCAGAGAAAGTCCTAGACCTCTTAAGCTCCCTCAAGGTCACAAAAGCAAGAATTTACGACACGAATCCTCAAGTACTAACAGCATTTGCCAATTCCGGCGTTGAGCTAATGGTGACAATCGAAAACGCAATGTTAGGCCAGTTGATGAACCCCCAAGCAGCCCTTCAATGGGTTAACGCCCACATCAAGCCTTATGTTCCCGCCACGAAAATATCCAGCATTGCGGTAGGGAATGAGGTCTTCACCGACGATGACACAACCGTGCTGCTAGATAATCTTGTCCCAGCCATGGTCAGCATTCAAGCGGCACTAACTCAGTTAGGCCTTGACTCAAACATTAAGGTCTCAACACCTTGCTCTTTAGCAGTGCTTGAAGAATCCTACCCACCTTCCGCGGGGAGCTTCAAAACTGAAGTAGCTGGTGTCATGTCACAATTGTTACAGTTTTTGCAAACCACAAAGGCACCTTTTTGGATCAATGCCTATCCCTACTTTGCATACAAAGATAGCCCGAATAAAATTTCTCTAGACTATGTGCTCTTCAATCCCAATTCGGGCATGATTGATCCTTATACCAAGTTGCACTATGACAATATGTTGTATGCTCAAGTAGATGCAGCCATTTTCGCTATGGCGCGGTTGGGGTTCAATGGGATCGAGGTCAAGGTTTCGGAGACGGGGTGGCCATCCAAGGGCGATGCCAATGAAATCGGTGCAACTTTGCAGAATGCTGCTATTTACAATAGAAACTTGCTGAGAAGGCAATTGGAAAATGAAGGCACACCTTTGAGGCCTAGAATGAGGCTTGAAATCTATGTTTTTGCTCTTTTCAATGAGAACATGAAGCCAGGACCAACTTCAGAGAGGAATTACGGTCTATTTCAACCGGATGGAACTATGGCTTACAATGTGGGATTGTCTGCCTTGTCAACTACTTCAACTACCTCATCAACCTCGACAGCTTCAATTTCTGATCTCACTTCCTCTGCCACAAAGCCAACAGCAATGGACTTTCATCAAAGCTGGGTGAGCTGCATCTTTGTTTGTGTGGTGACCTTCCAAGTTTATATGAGAAGACCATTTTAA

Protein Analysis

404

Amino Acids

44.38

Weight (kDa)

5.15

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 29 - 346 7.8e-81 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 589
AccII CGCG 2 cut(s) 521, 763
AciI CCGC 6 cut(s) 327, 350, 431, 519, 521, 763
AclWI GGATC 3 cut(s) 608, 686, 788
AcoI YGGCCR 2 cut(s) 100, 809
AcsI RAATTY 3 cut(s) 6, 177, 645
AcuI CTGAAG 2 cut(s) 558, 969
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
AflII CTTAAG 1 cut(s) 148
AjnI CCWGG 1 cut(s) 973
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AleI CACNNNNGTG 1 cut(s) 386
AluBI AGCT 7 cut(s) 153, 232, 528, 545, 1088, 1149, 1158
AluI AGCT 7 cut(s) 153, 232, 528, 545, 1088, 1149, 1158
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 2 cut(s) 475, 794
AlwI GGATC 3 cut(s) 608, 686, 788
AoxI GGCC 5 cut(s) 100, 262, 448, 809, 917
ApeKI GCWGC 5 cut(s) 284, 391, 743, 857, 1158
ApoI RAATTY 3 cut(s) 6, 177, 645
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 4 cut(s) 250, 361, 1165, 1189
AvaII GGWCC 1 cut(s) 977
BalI TGGCCA 2 cut(s) 102, 811
BanI GGYRCC 1 cut(s) 589
BbsI GAAGAC 2 cut(s) 358, 1207
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 5 cut(s) 296, 378, 755, 844, 1145
BccI CCATC 2 cut(s) 820, 1010
BciT130I CCWGG 1 cut(s) 975
BcoDI GTCTC 2 cut(s) 475, 794
BfaI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
BfrI CTTAAG 1 cut(s) 148
BglI GCCNNNNNGGC 1 cut(s) 108
BisI GCNGC 6 cut(s) 285, 392, 432, 744, 858, 1159
BlsI GCNGC 6 cut(s) 286, 393, 433, 745, 859, 1160
BmcAI AGTACT 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 975
Bme18I GGWCC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 1 cut(s) 975
BmsI GCATC 4 cut(s) 99, 730, 814, 1170
BpiI GAAGAC 2 cut(s) 358, 1207
BpuEI CTTGAG 3 cut(s) 143, 180, 717
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 3 cut(s) 414, 519, 816
BsaWI WCCGGW 1 cut(s) 1012
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 3 cut(s) 261, 345, 1137
BseBI CCWGG 1 cut(s) 975
BseDI CCNNGG 3 cut(s) 414, 519, 816
BseGI GGATG 2 cut(s) 812, 1021
BseLI CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
BseMI GCAATG 3 cut(s) 261, 345, 1137
BseMII CTCAG 3 cut(s) 75, 455, 872
BseNI ACTGG 1 cut(s) 265
BseXI GCAGC 5 cut(s) 296, 378, 755, 844, 1145
BseYI CCCAGC 2 cut(s) 409, 1149
Bsh1236I CGCG 2 cut(s) 521, 763
BshFI GGCC 5 cut(s) 102, 264, 450, 811, 919
BshNI GGYRCC 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 666
BsiSI CCGG 2 cut(s) 222, 1013
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
BsmAI GTCTC 2 cut(s) 475, 794
BsmBI CGTCTC 1 cut(s) 794
BsmFI GGGAC 1 cut(s) 392
BsmI GAATGC 2 cut(s) 423, 859
BsnI GGCC 5 cut(s) 102, 264, 450, 811, 919
Bso31I GGTCTC 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 4 cut(s) 600, 691, 780, 1099
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 6 cut(s) 327, 350, 431, 519, 521, 763
BspANI GGCC 5 cut(s) 102, 264, 450, 811, 919
BspCNI CTCAG 3 cut(s) 74, 454, 873
BspFNI CGCG 2 cut(s) 521, 763
BspLI GGNNCC 1 cut(s) 591
BspPI GGATC 3 cut(s) 608, 686, 788
BspQI GCTCTTC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 589
BspTI CTTAAG 1 cut(s) 148
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 3 cut(s) 261, 345, 1137
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 3 cut(s) 414, 519, 816
BssMI GATC 4 cut(s) 600, 691, 780, 1099
BssT1I CCWWGG 2 cut(s) 414, 816
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 3 cut(s) 388, 570, 1001
Bst6I CTCTTC 1 cut(s) 671
BstAFI CTTAAG 1 cut(s) 148
BstDEI CTNAG 3 cut(s) 61, 441, 881
BstDSI CCRYGG 2 cut(s) 414, 519
BstEII GGTNACC 1 cut(s) 1177
BstF5I GGATG 2 cut(s) 812, 1021
BstFNI CGCG 2 cut(s) 521, 763
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 4 cut(s) 603, 694, 783, 1102
BstMAI GTCTC 2 cut(s) 475, 794
BstMBI GATC 4 cut(s) 600, 691, 780, 1099
BstMWI GCNNNNNNNGC 4 cut(s) 108, 752, 886, 1155
BstNI CCWGG 1 cut(s) 975
BstPI GGTNACC 1 cut(s) 1177
BstSCI CCNGG 1 cut(s) 973
BstUI CGCG 2 cut(s) 521, 763
BstV1I GCAGC 5 cut(s) 296, 378, 755, 844, 1145
BstV2I GAAGAC 2 cut(s) 358, 1207
BsuRI GGCC 5 cut(s) 102, 264, 450, 811, 919
BtgI CCRYGG 2 cut(s) 414, 519
BtgZI GCGATG 1 cut(s) 837
BtsCI GGATG 2 cut(s) 812, 1021
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
CfoI GCGC 1 cut(s) 763
Cfr13I GGNCC 1 cut(s) 977
Cfr42I CCGCGG 1 cut(s) 522
Csp6I GTAC 1 cut(s) 200
CviAII CATG 4 cut(s) 415, 553, 685, 967
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 61, 441, 881
DpnI GATC 4 cut(s) 602, 693, 782, 1101
DpnII GATC 4 cut(s) 600, 691, 780, 1099
EaeI YGGCCR 2 cut(s) 100, 809
Eam1104I CTCTTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 671
Eco130I CCWWGG 2 cut(s) 414, 816
Eco147I AGGCCT 2 cut(s) 450, 919
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 977
Eco57I CTGAAG 2 cut(s) 558, 969
Eco91I GGTNACC 1 cut(s) 1177
EcoO65I GGTNACC 1 cut(s) 1177
EcoRII CCWGG 1 cut(s) 973
EcoT14I CCWWGG 2 cut(s) 414, 816
ErhI CCWWGG 2 cut(s) 414, 816
Esp3I CGTCTC 1 cut(s) 794
FaeI CATG 4 cut(s) 418, 556, 688, 970
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FaqI GGGAC 1 cut(s) 392
FatI CATG 4 cut(s) 414, 552, 684, 966
FauI CCCGC 2 cut(s) 334, 514
Fnu4HI GCNGC 6 cut(s) 285, 392, 432, 744, 858, 1159
FokI GGATG 2 cut(s) 799, 1028
Fsp4HI GCNGC 6 cut(s) 285, 392, 432, 744, 858, 1159
FspBI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
GlaI GCGC 1 cut(s) 762
GluI GCNGC 6 cut(s) 285, 392, 432, 744, 858, 1159
GsaI CCCAGC 2 cut(s) 413, 1153
HaeIII GGCC 5 cut(s) 102, 264, 450, 811, 919
HapII CCGG 2 cut(s) 222, 1013
HhaI GCGC 1 cut(s) 763
Hin1II CATG 4 cut(s) 418, 556, 688, 970
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 2 cut(s) 301, 1056
HindII GTYRAC 2 cut(s) 301, 1056
HinfI GANTC 4 cut(s) 27, 190, 455, 503
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 2 cut(s) 222, 1013
HphI GGTGA 4 cut(s) 250, 361, 1165, 1189
Hpy166II GTNNAC 2 cut(s) 301, 1056
Hpy188I TCNGA 4 cut(s) 64, 799, 988, 1099
Hpy188III TCNNGA 1 cut(s) 653
Hpy8I GTNNAC 2 cut(s) 301, 1056
Hpy99I CGWCG 1 cut(s) 377
HpyAV CCTTC 5 cut(s) 299, 525, 879, 896, 1192
HpyCH4III ACNGT 3 cut(s) 388, 570, 1001
HpyCH4V TGCA 7 cut(s) 577, 623, 709, 743, 842, 850, 1161
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 752, 886, 1155
HpyF3I CTNAG 3 cut(s) 61, 441, 881
Hsp92II CATG 4 cut(s) 418, 556, 688, 970
HspAI GCGC 1 cut(s) 761
KspAI GTTAAC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 522
Kzo9I GATC 4 cut(s) 600, 691, 780, 1099
LguI GCTCTTC 1 cut(s) 671
LmnI GCTCC 2 cut(s) 158, 525
Lsp1109I GCAGC 5 cut(s) 296, 378, 755, 844, 1145
LweI GCATC 4 cut(s) 99, 730, 814, 1170
MaeI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
MaeIII GTNAC 6 cut(s) 76, 163, 238, 555, 564, 1177
MalI GATC 4 cut(s) 602, 693, 782, 1101
MboI GATC 4 cut(s) 600, 691, 780, 1099
MboII GAAGA 5 cut(s) 32, 358, 512, 658, 1212
MfeI CAATTG 2 cut(s) 560, 890
MhlI GDGCHC 1 cut(s) 666
MlsI TGGCCA 2 cut(s) 102, 811
MluNI TGGCCA 2 cut(s) 102, 811
MlyI GAGTC 1 cut(s) 449
Mox20I TGGCCA 2 cut(s) 102, 811
MscI TGGCCA 2 cut(s) 102, 811
MseI TTAA 4 cut(s) 149, 300, 465, 1210
MslI CAYNNNNRTG 1 cut(s) 386
Msp20I TGGCCA 2 cut(s) 102, 811
MspA1I CMGCKG 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 148
MspI CCGG 2 cut(s) 222, 1013
MspR9I CCNGG 1 cut(s) 975
MunI CAATTG 2 cut(s) 560, 890
Mva1269I GAATGC 2 cut(s) 423, 859
MvaI CCWGG 1 cut(s) 975
MvnI CGCG 2 cut(s) 521, 763
MwoI GCNNNNNNNGC 4 cut(s) 108, 752, 886, 1155
NcoI CCATGG 1 cut(s) 414
NdeII GATC 4 cut(s) 600, 691, 780, 1099
NlaIII CATG 4 cut(s) 418, 556, 688, 970
NlaIV GGNNCC 1 cut(s) 591
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 4 cut(s) 163, 238, 555, 1177
OliI CACNNNNGTG 1 cut(s) 386
PceI AGGCCT 2 cut(s) 450, 919
PciSI GCTCTTC 1 cut(s) 671
PctI GAATGC 2 cut(s) 423, 859
PfeI GAWTC 3 cut(s) 27, 190, 503
PkrI GCNGC 6 cut(s) 286, 393, 433, 745, 859, 1160
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 973
PspEI GGTNACC 1 cut(s) 1177
PspFI CCCAGC 2 cut(s) 409, 1149
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 977
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 386
SacII CCGCGG 1 cut(s) 522
SapI GCTCTTC 1 cut(s) 671
SaqAI TTAA 4 cut(s) 149, 300, 465, 1210
SatI GCNGC 6 cut(s) 285, 392, 432, 744, 858, 1159
Sau3AI GATC 4 cut(s) 600, 691, 780, 1099
Sau96I GGNCC 1 cut(s) 977
ScaI AGTACT 1 cut(s) 201
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 975
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 4 cut(s) 99, 730, 814, 1170
Sfr303I CCGCGG 1 cut(s) 522
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 1 cut(s) 977
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 4 cut(s) 148, 158, 195, 732
SmoI CTYRAG 4 cut(s) 148, 158, 195, 732
SseBI AGGCCT 2 cut(s) 450, 919
SsiI CCGC 6 cut(s) 327, 350, 431, 519, 521, 763
SspMI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
StuI AGGCCT 2 cut(s) 450, 919
StyD4I CCNGG 1 cut(s) 973
StyI CCWWGG 2 cut(s) 414, 816
TaaI ACNGT 3 cut(s) 388, 570, 1001
TaqI TCGA 3 cut(s) 246, 783, 1082
TatI WGTACW 1 cut(s) 199
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 3 cut(s) 27, 190, 503
Tru1I TTAA 4 cut(s) 149, 300, 465, 1210
Tru9I TTAA 4 cut(s) 149, 300, 465, 1210
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 4 cut(s) 163, 238, 555, 1177
TseI GCWGC 5 cut(s) 284, 391, 743, 857, 1158
Tsp45I GTSAC 4 cut(s) 163, 238, 555, 1177
TspDTI ATGAA 5 cut(s) 287, 846, 915, 983, 1130
TspRI CASTG 1 cut(s) 498
Vha464I CTTAAG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 977
XapI RAATTY 3 cut(s) 6, 177, 645
XbaI TCTAGA 1 cut(s) 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XspI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
ZrmI AGTACT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.