Rw0G006270

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00245
OG0002223
Physical Location & Seq
Forward (+)
33330 .. 34664
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006270.1

Sequence Viewer

Length: 1335 bp
ATGCTTTGTTTTTCTAGTTATTTGTCAATGGCAAGTTTTAGAAGAATCAGTGCTGTTTTTCTTCTTCTGTGTTCGCTGATTTGTTTACTACATGGAGTTTCATCACTTGGCATAAACTATGGCCAACTCGGTGACAATTTGCTGCCACCACAGCAAGTTGTGAACCTCCTAACCGCCAACAACATCACCAAAGCAAGAATCTACGACACAAATCCACAAATATTAACTGCATTCGCCAATTCCAATATTGAGCTAATTGTGACGGTTGAAAATGAAGTGCTGGGAGAATTAACCAATTCCGAAGCAGGCCTTCAATGGGTTAGCACACACATCAAGCCTTATTTTCCCGCAACGAAAATCACAGGCATTGCTGTGGGGAACGAGATTGATGCAACGGATTTGCTACCTAACCTTGTACCGGCCATGTCCAACATTCAAGGGGCACTTTCTCAGCTAGGCCTTGACTCACACATCAAGGTGTCATCGTCTTTTTCTTATTCGGTGATCAAAGAGTCTTATCCGCCTTCTGCTGGCAGTTTCAAAAGCGAGCTCTCCAGTGTAATGTCACAATTGTTACAGTTTCTGTCGAGCACAAAGGCACCATTTTGGATCAATGCATATCCTTACTTTGCATACAAGGGCAACTCGAAAGAAATTCCTCTAGACTATGTGCTTTTCAGTAATGCCGGTTCATCAGCAGGAGTGACTGACCCTACTAGCAATTTGCACTATGACAACATGTTGTATGCTCAAGTAGATGCAGTCACTTATGCCATTTCTAAATTGGGCTTCGATGGCATAGAGGTTAGGGTCGCAGAGACAGGGTGGCCATCAAAAGGGGATGTTGGTGAACTTGGTGCAAGTTTAGAGAATGCTGCTATATACAATGGGAACTTGTTGAAGAGGCAATTGGCCAACGAAGGTACACCTTTGAAGCCTAAGCTTGACGTCTATATTTTCGCTCTTTTTAATGAGAACAAGAAGCCTGGACCAACATCGGAGAGGAATTTTGGTCTATTTCAACCGGATGGAACTTCGGCTTACAATGTGGGCTTGTCTGGATCATCATCTACCTCAACTAATGCCCCATCACCCCCATCGCCAAGCCTATCAAAACCACCAACTTCAGCTACCAACTCAACTGGTGCCTCATCATCACCCTCAATCCCATTAAAATCATCAAGCTCGGCTACTAATTCAACTAATGCCTCATCCCCCTCGTCACTTCCTGAGATTGGTATGACTTATTCCTCTGGTGCCAAGGCAGCAACAATGGGAAGCTTGGTTTACATTTTTCTCTGTGTATTGACCTTCCAATTTTGTATGATGATCTAG

Protein Analysis

444

Amino Acids

47.21

Weight (kDa)

5.47

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 36 - 350 4.2e-82 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 951
AccB1I GGYRCC 3 cut(s) 598, 1145, 1256
AciI CCGC 3 cut(s) 174, 348, 521
AclWI GGATC 2 cut(s) 617, 1069
AcoI YGGCCR 4 cut(s) 121, 420, 827, 912
AcsI RAATTY 2 cut(s) 654, 1006
AcuI CTGAAG 1 cut(s) 1110
AcyI GRCGYC 1 cut(s) 948
AfaI GTAC 2 cut(s) 417, 925
AfiI CCNNNNNNNGG 5 cut(s) 316, 418, 530, 836, 1235
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 8 cut(s) 269, 314, 437, 541, 901, 934, 1022, 1200
AjnI CCWGG 1 cut(s) 985
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AluBI AGCT 7 cut(s) 253, 454, 550, 943, 1130, 1185, 1281
AluI AGCT 7 cut(s) 253, 454, 550, 943, 1130, 1185, 1281
Alw21I GWGCWC 2 cut(s) 552, 593
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 2 cut(s) 617, 1069
AlwNI CAGNNNCTG 1 cut(s) 583
AoxI GGCC 6 cut(s) 121, 307, 420, 457, 827, 912
ApeKI GCWGC 3 cut(s) 142, 875, 1265
ApoI RAATTY 2 cut(s) 654, 1006
AspS9I GGNCC 1 cut(s) 989
AsuHPI GGTGA 6 cut(s) 143, 178, 514, 860, 1083, 1149
AvaII GGWCC 1 cut(s) 989
BaeGI GKGCMC 1 cut(s) 445
BalI TGGCCA 3 cut(s) 123, 829, 914
BanI GGYRCC 3 cut(s) 598, 1145, 1256
BanII GRGCYC 1 cut(s) 552
Bbv12I GWGCWC 2 cut(s) 552, 593
BbvI GCAGC 3 cut(s) 129, 862, 1277
BccI CCATC 5 cut(s) 788, 838, 1022, 1096, 1105
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 987
BclI TGATCA 1 cut(s) 504
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 5 cut(s) 15, 455, 662, 717, 1333
BisI GCNGC 3 cut(s) 143, 876, 1266
BlsI GCNGC 3 cut(s) 144, 877, 1267
Bme1390I CCNGG 1 cut(s) 987
Bme18I GGWCC 1 cut(s) 989
BmgT120I GGNCC 1 cut(s) 989
BmiI GGNNCC 3 cut(s) 600, 1147, 1258
BmrFI CCNGG 1 cut(s) 987
BmsI GCATC 2 cut(s) 379, 748
BpmI CTGGAG 1 cut(s) 538
Bpu10I CCTNAGC 1 cut(s) 939
BpuEI CTTGAG 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 1066
BsaHI GRCGYC 1 cut(s) 948
BsaJI CCNNGG 1 cut(s) 1260
BsaWI WCCGGW 1 cut(s) 1024
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 418, 530, 836, 1235
Bse118I RCCGGY 2 cut(s) 418, 686
Bse1I ACTGG 2 cut(s) 555, 1147
Bse3DI GCAATG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 1066
BseBI CCWGG 1 cut(s) 987
BseDI CCNNGG 1 cut(s) 1260
BseGI GGATG 3 cut(s) 847, 1033, 1211
BseJI GATNNNNATC 1 cut(s) 1066
BseLI CCNNNNNNNGG 5 cut(s) 316, 418, 530, 836, 1235
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 464, 1221
BseNI ACTGG 2 cut(s) 555, 1147
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 3 cut(s) 129, 862, 1277
BseYI CCCAGC 1 cut(s) 280
BshFI GGCC 6 cut(s) 123, 309, 422, 459, 829, 914
BshNI GGYRCC 3 cut(s) 598, 1145, 1256
BsiHKAI GWGCWC 2 cut(s) 552, 593
BsiSI CCGG 3 cut(s) 419, 687, 1025
BslI CCNNNNNNNGG 5 cut(s) 316, 418, 530, 836, 1235
BsmAI GTCTC 1 cut(s) 812
BsmI GAATGC 2 cut(s) 230, 877
BsnI GGCC 6 cut(s) 123, 309, 422, 459, 829, 914
Bsp1286I GDGCHC 3 cut(s) 445, 552, 593
Bsp143I GATC 4 cut(s) 504, 609, 1061, 1329
BspACI CCGC 3 cut(s) 174, 348, 521
BspANI GGCC 6 cut(s) 123, 309, 422, 459, 829, 914
BspCNI CTCAG 2 cut(s) 463, 1222
BspLI GGNNCC 3 cut(s) 600, 1147, 1258
BspPI GGATC 2 cut(s) 617, 1069
BspT107I GGYRCC 3 cut(s) 598, 1145, 1256
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 2 cut(s) 418, 686
BsrI ACTGG 2 cut(s) 555, 1147
BssAI RCCGGY 2 cut(s) 418, 686
BssECI CCNNGG 1 cut(s) 1260
BssMI GATC 4 cut(s) 504, 609, 1061, 1329
BssNI GRCGYC 1 cut(s) 948
BssT1I CCWWGG 1 cut(s) 1260
Bst2UI CCWGG 1 cut(s) 987
Bst4CI ACNGT 2 cut(s) 265, 579
Bst6I CTCTTC 1 cut(s) 896
BstACI GRCGYC 1 cut(s) 948
BstC8I GCNNGC 3 cut(s) 307, 532, 548
BstDEI CTNAG 3 cut(s) 450, 939, 1230
BstF5I GGATG 3 cut(s) 847, 1033, 1211
BstKTI GATC 4 cut(s) 507, 612, 1064, 1332
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 4 cut(s) 504, 609, 1061, 1329
BstMWI GCNNNNNNNGC 3 cut(s) 151, 795, 1265
BstNI CCWGG 1 cut(s) 987
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 985
BstSLI GKGCMC 1 cut(s) 445
BstV1I GCAGC 3 cut(s) 129, 862, 1277
BsuRI GGCC 6 cut(s) 123, 309, 422, 459, 829, 914
BtgZI GCGATG 1 cut(s) 1083
BtsCI GGATG 3 cut(s) 847, 1033, 1211
BtsIMutI CAGTG 2 cut(s) 55, 562
Cac8I GCNNGC 3 cut(s) 307, 532, 548
CaiI CAGNNNCTG 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 418, 686
Cfr13I GGNCC 1 cut(s) 989
Csp6I GTAC 2 cut(s) 416, 924
CviAII CATG 3 cut(s) 92, 424, 739
CviQI GTAC 2 cut(s) 416, 924
DdeI CTNAG 3 cut(s) 450, 939, 1230
DpnI GATC 4 cut(s) 506, 611, 1063, 1331
DpnII GATC 4 cut(s) 504, 609, 1061, 1329
EaeI YGGCCR 4 cut(s) 121, 420, 827, 912
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
EciI GGCGGA 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 550
Eco130I CCWWGG 1 cut(s) 1260
Eco147I AGGCCT 2 cut(s) 309, 459
Eco24I GRGCYC 1 cut(s) 552
Eco47I GGWCC 1 cut(s) 989
Eco53kI GAGCTC 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 1110
EcoICRI GAGCTC 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 1260
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 1260
FaeI CATG 3 cut(s) 95, 427, 742
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 429, 461
FatI CATG 3 cut(s) 91, 423, 738
FauI CCCGC 1 cut(s) 355
FbaI TGATCA 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 143, 876, 1266
FokI GGATG 3 cut(s) 854, 1040, 1198
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 3 cut(s) 143, 876, 1266
FspBI CTAG 5 cut(s) 15, 455, 662, 717, 1333
GluI GCNGC 3 cut(s) 143, 876, 1266
GsaI CCCAGC 1 cut(s) 284
GsuI CTGGAG 1 cut(s) 538
HaeIII GGCC 6 cut(s) 123, 309, 422, 459, 829, 914
HapII CCGG 3 cut(s) 419, 687, 1025
Hin1I GRCGYC 1 cut(s) 948
Hin1II CATG 3 cut(s) 95, 427, 742
HindIII AAGCTT 2 cut(s) 941, 1279
HinfI GANTC 4 cut(s) 45, 198, 464, 512
HpaII CCGG 3 cut(s) 419, 687, 1025
HphI GGTGA 6 cut(s) 143, 178, 514, 860, 1083, 1149
Hpy166II GTNNAC 5 cut(s) 86, 163, 851, 926, 1288
Hpy188I TCNGA 2 cut(s) 301, 1000
Hpy188III TCNNGA 3 cut(s) 662, 1059, 1229
Hpy8I GTNNAC 5 cut(s) 86, 163, 851, 926, 1288
HpyAV CCTTC 4 cut(s) 320, 534, 914, 1321
HpyCH4III ACNGT 2 cut(s) 265, 579
HpyCH4IV ACGT 1 cut(s) 948
HpyCH4V TGCA 7 cut(s) 230, 392, 617, 632, 727, 761, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 795, 1265
HpyF3I CTNAG 3 cut(s) 450, 939, 1230
HpySE526I ACGT 1 cut(s) 948
Hsp92I GRCGYC 1 cut(s) 948
Hsp92II CATG 3 cut(s) 95, 427, 742
Ksp22I TGATCA 1 cut(s) 504
Kzo9I GATC 4 cut(s) 504, 609, 1061, 1329
Lsp1109I GCAGC 3 cut(s) 129, 862, 1277
LweI GCATC 2 cut(s) 379, 748
MaeI CTAG 5 cut(s) 15, 455, 662, 717, 1333
MaeII ACGT 1 cut(s) 948
MaeIII GTNAC 7 cut(s) 131, 259, 564, 573, 703, 763, 1221
MalI GATC 4 cut(s) 506, 611, 1063, 1331
MboI GATC 4 cut(s) 504, 609, 1061, 1329
MboII GAAGA 4 cut(s) 53, 54, 56, 913
MfeI CAATTG 2 cut(s) 569, 908
MhlI GDGCHC 3 cut(s) 445, 552, 593
MlsI TGGCCA 3 cut(s) 123, 829, 914
MluNI TGGCCA 3 cut(s) 123, 829, 914
MlyI GAGTC 2 cut(s) 458, 521
MmeI TCCRAC 1 cut(s) 453
Mox20I TGGCCA 3 cut(s) 123, 829, 914
Mph1103I ATGCAT 1 cut(s) 619
MscI TGGCCA 3 cut(s) 123, 829, 914
MseI TTAA 4 cut(s) 224, 290, 969, 1172
MslI CAYNNNNRTG 2 cut(s) 371, 476
Msp20I TGGCCA 3 cut(s) 123, 829, 914
MspI CCGG 3 cut(s) 419, 687, 1025
MspR9I CCNGG 1 cut(s) 987
MunI CAATTG 2 cut(s) 569, 908
Mva1269I GAATGC 2 cut(s) 230, 877
MvaI CCWGG 1 cut(s) 987
MwoI GCNNNNNNNGC 3 cut(s) 151, 795, 1265
NdeII GATC 4 cut(s) 504, 609, 1061, 1329
NlaIII CATG 3 cut(s) 95, 427, 742
NlaIV GGNNCC 3 cut(s) 600, 1147, 1258
NmeAIII GCCGAG 1 cut(s) 1166
NmuCI GTSAC 6 cut(s) 131, 259, 564, 703, 763, 1221
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 742
PceI AGGCCT 2 cut(s) 309, 459
PciI ACATGT 1 cut(s) 738
PctI GAATGC 2 cut(s) 230, 877
PfeI GAWTC 2 cut(s) 45, 198
PkrI GCNGC 3 cut(s) 144, 877, 1267
PleI GAGTC 2 cut(s) 458, 520
PpsI GAGTC 2 cut(s) 458, 520
PscI ACATGT 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 552
Psp6I CCWGG 1 cut(s) 985
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 1 cut(s) 985
PspN4I GGNNCC 3 cut(s) 600, 1147, 1258
PspPI GGNCC 1 cut(s) 989
PsrI GAACNNNNNNTAC 2 cut(s) 673, 705
PstNI CAGNNNCTG 1 cut(s) 583
RsaI GTAC 2 cut(s) 417, 925
RsaNI GTAC 2 cut(s) 416, 924
RseI CAYNNNNRTG 2 cut(s) 371, 476
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 4 cut(s) 224, 290, 969, 1172
SatI GCNGC 3 cut(s) 143, 876, 1266
Sau3AI GATC 4 cut(s) 504, 609, 1061, 1329
Sau96I GGNCC 1 cut(s) 989
SchI GAGTC 2 cut(s) 458, 521
ScrFI CCNGG 1 cut(s) 987
SduI GDGCHC 3 cut(s) 445, 552, 593
SfaNI GCATC 2 cut(s) 379, 748
SinI GGWCC 1 cut(s) 989
SmiMI CAYNNNNRTG 2 cut(s) 371, 476
SmlI CTYRAG 1 cut(s) 750
SmoI CTYRAG 1 cut(s) 750
SseBI AGGCCT 2 cut(s) 309, 459
SsiI CCGC 3 cut(s) 174, 348, 521
SspI AATATT 2 cut(s) 222, 247
SspMI CTAG 5 cut(s) 15, 455, 662, 717, 1333
SstI GAGCTC 1 cut(s) 552
StuI AGGCCT 2 cut(s) 309, 459
StyD4I CCNGG 1 cut(s) 985
StyI CCWWGG 1 cut(s) 1260
TaaI ACNGT 2 cut(s) 265, 579
TaiI ACGT 1 cut(s) 951
TaqI TCGA 3 cut(s) 587, 647, 792
TfiI GAWTC 2 cut(s) 45, 198
Tru1I TTAA 4 cut(s) 224, 290, 969, 1172
Tru9I TTAA 4 cut(s) 224, 290, 969, 1172
TscAI CASTG 2 cut(s) 55, 562
TseFI GTSAC 6 cut(s) 131, 259, 564, 703, 763, 1221
TseI GCWGC 3 cut(s) 142, 875, 1265
Tsp45I GTSAC 6 cut(s) 131, 259, 564, 703, 763, 1221
TspDTI ATGAA 3 cut(s) 90, 288, 681
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 2 cut(s) 55, 562
VpaK11BI GGWCC 1 cut(s) 989
XapI RAATTY 2 cut(s) 654, 1006
XbaI TCTAGA 1 cut(s) 661
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XspI CTAG 5 cut(s) 15, 455, 662, 717, 1333
ZraI GACGTC 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.