RchiOBHm_Chr4g0438021

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002223
Physical Location & Seq
Forward (+)
60945870 .. 60946774
905 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40621

Sequence Viewer

Length: 669 bp
ATGGCAATGTGTACCAGAATCAGTGCTGCTTTTCTTCTTCTATGTTGGTTGATATGTTTCTCAGGGTTTGGTTTCGTACAAAGAGTTGCATCGCTTGGTATCAACTTCGGCCAACTCGGCGACAATTTGCTACAACCACAGCAAGTTATGAGCCTCTTAAGGTCCAACAATATCACAAAAACAAGAATTTACGACACCAATCCGCAGATACCAGCTGCATTCGCAAATTCGGATATTGAGCTCATTGTGACAGTGGAAAACGAAATGCTAAGCCAATTAACCAACCCCCAAGCAGCCCTTCAATGGGTTACTACACACATCAAGCCTTATTTTCTCTCCACGAAAATCACAGGCATTATGATAGGGAACGAGATTGATACAACGGATTTGCTAGCTAGCCTTGTACCGGCCGTGTCCAGCATTCAAAAGGCCCTATCTCAGTTAGGCCTTGATTCGCACATGAAGTCTTATCCACCTTCTGCTGGCAGTTTCAAAAGCGAGGTCTCGGGTGTGATGTCACAATTGTTACAGTTTTTGTCAAGCACAAAGGCGCCATTTTGGATCAATGCATATCCTTACTTTGCGTACAAGGGCAACTCGAAAGATATTCCTCTAGACCATGTGCGTTTCAGTAATGCCGGTTCATCAGCAGGAGTGACTGACCCTTAG

Protein Analysis

222

Amino Acids

24.22

Weight (kDa)

6.81

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 32 - 216 1.5e-33 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 550
AciI CCGC 1 cut(s) 203
AclWI GGATC 1 cut(s) 569
AcoI YGGCCR 2 cut(s) 109, 408
AcsI RAATTY 2 cut(s) 186, 226
AcyI GRCGYC 1 cut(s) 551
AfaI GTAC 4 cut(s) 13, 78, 405, 587
AfiI CCNNNNNNNGG 4 cut(s) 303, 304, 406, 482
AflII CTTAAG 1 cut(s) 157
AgsI TTSAA 3 cut(s) 302, 425, 493
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AluBI AGCT 3 cut(s) 215, 241, 395
AluI AGCT 3 cut(s) 215, 241, 395
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 508
AlwI GGATC 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 505
AoxI GGCC 4 cut(s) 109, 408, 429, 445
ApeKI GCWGC 3 cut(s) 26, 215, 293
ApoI RAATTY 2 cut(s) 186, 226
ArsI GACNNNNNNTTYG 2 cut(s) 486, 518
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 2 cut(s) 162, 430
AsuNHI GCTAGC 2 cut(s) 391, 395
AvaI CYCGRG 1 cut(s) 505
AvaII GGWCC 1 cut(s) 162
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 13, 202, 305
BceAI ACGGC 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 3 cut(s) 392, 396, 614
BfoI RGCGCY 1 cut(s) 554
BfrI CTTAAG 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 3 cut(s) 27, 216, 294
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 3 cut(s) 28, 217, 295
Bme18I GGWCC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 505
BmgT120I GGNCC 2 cut(s) 162, 430
BmiI GGNNCC 1 cut(s) 552
BmsI GCATC 1 cut(s) 98
BmtI GCTAGC 2 cut(s) 395, 399
Bpu1102I GCTNAGC 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 508
Bsc4I CCNNNNNNNGG 4 cut(s) 303, 304, 406, 482
Bse118I RCCGGY 2 cut(s) 406, 638
Bse3DI GCAATG 1 cut(s) 12
BseLI CCNNNNNNNGG 4 cut(s) 303, 304, 406, 482
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 75, 452
BseX3I CGGCCG 1 cut(s) 408
BseXI GCAGC 3 cut(s) 13, 202, 305
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 4 cut(s) 111, 410, 431, 447
BshNI GGYRCC 1 cut(s) 550
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 505
BsiSI CCGG 2 cut(s) 407, 639
BslI CCNNNNNNNGG 4 cut(s) 303, 304, 406, 482
BsmAI GTCTC 1 cut(s) 508
BsmI GAATGC 2 cut(s) 218, 420
BsnI GGCC 4 cut(s) 111, 410, 431, 447
Bso31I GGTCTC 1 cut(s) 508
BsoBI CYCGRG 1 cut(s) 505
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 1 cut(s) 561
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 1 cut(s) 203
BspANI GGCC 4 cut(s) 111, 410, 431, 447
BspCNI CTCAG 2 cut(s) 74, 451
BspLI GGNNCC 1 cut(s) 552
BspOI GCTAGC 2 cut(s) 395, 399
BspPI GGATC 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 550
BspTI CTTAAG 1 cut(s) 157
BspTNI GGTCTC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 406, 638
BssAI RCCGGY 2 cut(s) 406, 638
BssMI GATC 1 cut(s) 561
BssNI GRCGYC 1 cut(s) 551
Bst4CI ACNGT 2 cut(s) 253, 531
BstACI GRCGYC 1 cut(s) 551
BstAFI CTTAAG 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 393, 397, 484
BstDEI CTNAG 4 cut(s) 61, 269, 438, 666
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 1 cut(s) 564
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 1 cut(s) 561
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 2 cut(s) 117, 221
BstV1I GCAGC 3 cut(s) 13, 202, 305
BstZI CGGCCG 1 cut(s) 408
BsuRI GGCC 4 cut(s) 111, 410, 431, 447
BtgZI GCGATG 1 cut(s) 75
BtsIMutI CAGTG 2 cut(s) 28, 258
Cac8I GCNNGC 3 cut(s) 393, 397, 484
CfoI GCGC 1 cut(s) 553
Cfr10I RCCGGY 2 cut(s) 406, 638
Cfr13I GGNCC 2 cut(s) 162, 430
Csp6I GTAC 4 cut(s) 12, 77, 404, 586
CviAII CATG 2 cut(s) 460, 620
CviQI GTAC 4 cut(s) 12, 77, 404, 586
DdeI CTNAG 4 cut(s) 61, 269, 438, 666
DinI GGCGCC 1 cut(s) 552
DpnI GATC 1 cut(s) 563
DpnII GATC 1 cut(s) 561
EaeI YGGCCR 2 cut(s) 109, 408
EagI CGGCCG 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 408
Eco147I AGGCCT 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 162
Eco52I CGGCCG 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 505
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 430
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 243
EgeI GGCGCC 1 cut(s) 552
EheI GGCGCC 1 cut(s) 552
FaeI CATG 2 cut(s) 463, 623
FaiI YATR 7 cut(s) 43, 55, 149, 359, 461, 571, 621
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FatI CATG 2 cut(s) 459, 619
Fnu4HI GCNGC 3 cut(s) 27, 216, 294
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 3 cut(s) 27, 216, 294
FspBI CTAG 3 cut(s) 392, 396, 614
GlaI GCGC 1 cut(s) 552
GluI GCNGC 3 cut(s) 27, 216, 294
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 4 cut(s) 111, 410, 431, 447
HapII CCGG 2 cut(s) 407, 639
HhaI GCGC 1 cut(s) 553
Hin1I GRCGYC 1 cut(s) 551
Hin1II CATG 2 cut(s) 463, 623
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HinfI GANTC 2 cut(s) 18, 452
HpaII CCGG 2 cut(s) 407, 639
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 1 cut(s) 614
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 2 cut(s) 308, 486
HpyCH4III ACNGT 2 cut(s) 253, 531
HpyCH4V TGCA 3 cut(s) 89, 218, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 221
HpyF3I CTNAG 4 cut(s) 61, 269, 438, 666
Hsp92I GRCGYC 1 cut(s) 551
Hsp92II CATG 2 cut(s) 463, 623
HspAI GCGC 1 cut(s) 551
KasI GGCGCC 1 cut(s) 550
Kzo9I GATC 1 cut(s) 561
LpnPI CCDG 9 cut(s) 28, 48, 225, 336, 420, 430, 468, 636, 652
Lsp1109I GCAGC 3 cut(s) 13, 202, 305
LweI GCATC 1 cut(s) 98
MaeI CTAG 3 cut(s) 392, 396, 614
MaeIII GTNAC 5 cut(s) 247, 307, 516, 525, 655
MalI GATC 1 cut(s) 563
MboI GATC 1 cut(s) 561
MboII GAAGA 2 cut(s) 26, 29
MfeI CAATTG 1 cut(s) 521
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 5 cut(s) 124, 186, 226, 275, 521
Mly113I GGCGCC 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 3 cut(s) 164, 493, 621
Mph1103I ATGCAT 1 cut(s) 571
MseI TTAA 2 cut(s) 158, 278
MspA1I CMGCKG 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 157
MspI CCGG 2 cut(s) 407, 639
MunI CAATTG 1 cut(s) 521
Mva1269I GAATGC 2 cut(s) 218, 420
MwoI GCNNNNNNNGC 2 cut(s) 117, 221
NarI GGCGCC 1 cut(s) 551
NdeII GATC 1 cut(s) 561
NheI GCTAGC 2 cut(s) 391, 395
NlaIII CATG 2 cut(s) 463, 623
NlaIV GGNNCC 1 cut(s) 552
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 3 cut(s) 247, 516, 655
NsiI ATGCAT 1 cut(s) 571
PceI AGGCCT 1 cut(s) 447
PctI GAATGC 2 cut(s) 218, 420
PfeI GAWTC 2 cut(s) 18, 452
PkrI GCNGC 3 cut(s) 28, 217, 295
PluTI GGCGCC 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 552
PspPI GGNCC 2 cut(s) 162, 430
PsrI GAACNNNNNNTAC 2 cut(s) 625, 657
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 4 cut(s) 13, 78, 405, 587
RsaNI GTAC 4 cut(s) 12, 77, 404, 586
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 158, 278
SatI GCNGC 3 cut(s) 27, 216, 294
Sau3AI GATC 1 cut(s) 561
Sau96I GGNCC 2 cut(s) 162, 430
SduI GDGCHC 1 cut(s) 243
SetI ASST 6 cut(s) 164, 217, 243, 397, 478, 504
SfaNI GCATC 1 cut(s) 98
SfoI GGCGCC 1 cut(s) 552
SinI GGWCC 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 5 cut(s) 124, 186, 226, 275, 521
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 1 cut(s) 203
SspDI GGCGCC 1 cut(s) 550
SspMI CTAG 3 cut(s) 392, 396, 614
SstI GAGCTC 1 cut(s) 243
StuI AGGCCT 1 cut(s) 447
TaaI ACNGT 2 cut(s) 253, 531
TaqI TCGA 1 cut(s) 599
TasI AATT 5 cut(s) 124, 186, 226, 275, 521
TfiI GAWTC 2 cut(s) 18, 452
Tru1I TTAA 2 cut(s) 158, 278
Tru9I TTAA 2 cut(s) 158, 278
TscAI CASTG 2 cut(s) 28, 258
TseFI GTSAC 3 cut(s) 247, 516, 655
TseI GCWGC 3 cut(s) 26, 215, 293
Tsp45I GTSAC 3 cut(s) 247, 516, 655
TspDTI ATGAA 2 cut(s) 476, 633
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 2 cut(s) 28, 258
Vha464I CTTAAG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 2 cut(s) 186, 226
XbaI TCTAGA 1 cut(s) 613
XspI CTAG 3 cut(s) 392, 396, 614
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.