Rh4AG357900

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002223
Physical Location & Seq
Forward (+)
66818975 .. 66819881
907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG357900.1

Sequence Viewer

Length: 597 bp
ATGGCAATGTGTACCAGAATCAGTGCTGCTTTTCTTCTTCTATGTTGGTTGATATGTTTCTCAGGGTTTGGTTTCGTACAAAGAGTTGCATCGCTTGGTATCAACTTCGGCCAACTCGGCGACAATTTGCTACAACCACAGCAAGTTATGAGCCTCTTAAGGTCCAACAATATCACAAAAACAAGAATTTACGACACCAATCCGCAGATACCAGCTGCATTCGCAAATTCGGATATTGAGCTCATTGTGACAGTGGAAAACGAAATGCTAAGCCAATTAACCAACCCCCAAGCAGCCCTTCAATGGGTTACTACACACATCAAGCCTTATTTTCTCTCCACGAAAATCACAGGCATTATGATAGGGAACGAGATTGATACAACGGATTTGCTAGCTAGCCTTGTACCGGCCGTGTCCAGCATTCAAAAGGCCCTATCTCAGTTAGGCCTTGATTCGCACATGAAGGTGTCATCGCCTTTTTCTTTTTCGAGGTTAGGGTCGCGGAGACAGGGTGGCAAATCAAAAGGGGATGCTGGTGAACTTGGTGCAAGTTTGGAGAATGCTGCTATATACAATGGGAACTTGTTGAAGAGGTAA

Protein Analysis

198

Amino Acids

21.5

Weight (kDa)

8.86

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 32 - 168 7.6e-24 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 502
AciI CCGC 2 cut(s) 203, 502
AcoI YGGCCR 2 cut(s) 109, 408
AcsI RAATTY 2 cut(s) 186, 226
AfaI GTAC 3 cut(s) 13, 78, 405
AfiI CCNNNNNNNGG 3 cut(s) 303, 304, 406
AflII CTTAAG 1 cut(s) 157
AgsI TTSAA 3 cut(s) 302, 425, 589
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AluBI AGCT 3 cut(s) 215, 241, 395
AluI AGCT 3 cut(s) 215, 241, 395
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 499
AoxI GGCC 4 cut(s) 109, 408, 429, 445
ApeKI GCWGC 4 cut(s) 26, 215, 293, 563
ApoI RAATTY 2 cut(s) 186, 226
AspS9I GGNCC 2 cut(s) 162, 430
AsuHPI GGTGA 1 cut(s) 548
AsuNHI GCTAGC 2 cut(s) 391, 395
AvaII GGWCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 13, 202, 305, 550
BceAI ACGGC 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 499
BfaI CTAG 2 cut(s) 392, 396
BfrI CTTAAG 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 4 cut(s) 27, 216, 294, 564
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 4 cut(s) 28, 217, 295, 565
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 162, 430
BmsI GCATC 2 cut(s) 98, 520
BmtI GCTAGC 2 cut(s) 395, 399
Bpu1102I GCTNAGC 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 304, 406
Bse118I RCCGGY 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 12
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 3 cut(s) 303, 304, 406
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 75, 452
BseX3I CGGCCG 1 cut(s) 408
BseXI GCAGC 4 cut(s) 13, 202, 305, 550
Bsh1236I CGCG 1 cut(s) 502
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 4 cut(s) 111, 410, 431, 447
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 1 cut(s) 407
BslI CCNNNNNNNGG 3 cut(s) 303, 304, 406
BsmAI GTCTC 1 cut(s) 499
BsmI GAATGC 3 cut(s) 218, 420, 565
BsnI GGCC 4 cut(s) 111, 410, 431, 447
Bsp1286I GDGCHC 1 cut(s) 243
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 2 cut(s) 203, 502
BspANI GGCC 4 cut(s) 111, 410, 431, 447
BspCNI CTCAG 2 cut(s) 74, 451
BspFNI CGCG 1 cut(s) 502
BspOI GCTAGC 2 cut(s) 395, 399
BspTI CTTAAG 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 584
BstAFI CTTAAG 1 cut(s) 157
BstC8I GCNNGC 2 cut(s) 393, 397
BstDEI CTNAG 3 cut(s) 61, 269, 438
BstF5I GGATG 1 cut(s) 535
BstFNI CGCG 1 cut(s) 502
BstMAI GTCTC 1 cut(s) 499
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 2 cut(s) 117, 221
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 4 cut(s) 13, 202, 305, 550
BstZI CGGCCG 1 cut(s) 408
BsuRI GGCC 4 cut(s) 111, 410, 431, 447
BtgZI GCGATG 2 cut(s) 75, 456
BtsCI GGATG 1 cut(s) 535
BtsIMutI CAGTG 2 cut(s) 28, 258
Cac8I GCNNGC 2 cut(s) 393, 397
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 162, 430
Csp6I GTAC 3 cut(s) 12, 77, 404
CviAII CATG 1 cut(s) 460
CviQI GTAC 3 cut(s) 12, 77, 404
DdeI CTNAG 3 cut(s) 61, 269, 438
EaeI YGGCCR 2 cut(s) 109, 408
EagI CGGCCG 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 408
Eco147I AGGCCT 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 162
Eco52I CGGCCG 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 1 cut(s) 463
FaiI YATR 7 cut(s) 43, 55, 149, 359, 461, 569, 571
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FatI CATG 1 cut(s) 459
Fnu4HI GCNGC 4 cut(s) 27, 216, 294, 564
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 4 cut(s) 27, 216, 294, 564
FspBI CTAG 2 cut(s) 392, 396
GluI GCNGC 4 cut(s) 27, 216, 294, 564
HaeIII GGCC 4 cut(s) 111, 410, 431, 447
HapII CCGG 1 cut(s) 407
Hin1II CATG 1 cut(s) 463
HinfI GANTC 2 cut(s) 18, 452
HpaII CCGG 1 cut(s) 407
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 2 cut(s) 12, 539
Hpy188I TCNGA 1 cut(s) 232
Hpy8I GTNNAC 2 cut(s) 12, 539
HpyAV CCTTC 2 cut(s) 308, 457
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4V TGCA 3 cut(s) 89, 218, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 221
HpyF3I CTNAG 3 cut(s) 61, 269, 438
Hsp92II CATG 1 cut(s) 463
LpnPI CCDG 8 cut(s) 28, 48, 225, 336, 420, 430, 494, 519
Lsp1109I GCAGC 4 cut(s) 13, 202, 305, 550
LweI GCATC 2 cut(s) 98, 520
MaeI CTAG 2 cut(s) 392, 396
MaeIII GTNAC 2 cut(s) 247, 307
MboII GAAGA 2 cut(s) 26, 29
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 4 cut(s) 124, 186, 226, 275
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 3 cut(s) 164, 483, 585
MseI TTAA 2 cut(s) 158, 278
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 157
MspI CCGG 1 cut(s) 407
Mva1269I GAATGC 3 cut(s) 218, 420, 565
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 117, 221
NheI GCTAGC 2 cut(s) 391, 395
NlaIII CATG 1 cut(s) 463
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 247
PceI AGGCCT 1 cut(s) 447
PctI GAATGC 3 cut(s) 218, 420, 565
PfeI GAWTC 2 cut(s) 18, 452
PkrI GCNGC 4 cut(s) 28, 217, 295, 565
Psp124BI GAGCTC 1 cut(s) 243
PspPI GGNCC 2 cut(s) 162, 430
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 3 cut(s) 13, 78, 405
RsaNI GTAC 3 cut(s) 12, 77, 404
RseI CAYNNNNRTG 1 cut(s) 464
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 158, 278
SatI GCNGC 4 cut(s) 27, 216, 294, 564
Sau96I GGNCC 2 cut(s) 162, 430
SduI GDGCHC 1 cut(s) 243
SetI ASST 7 cut(s) 164, 217, 243, 397, 468, 494, 596
SfaNI GCATC 2 cut(s) 98, 520
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 4 cut(s) 124, 186, 226, 275
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 2 cut(s) 203, 502
SspMI CTAG 2 cut(s) 392, 396
SstI GAGCTC 1 cut(s) 243
StuI AGGCCT 1 cut(s) 447
TaaI ACNGT 1 cut(s) 253
TaqI TCGA 1 cut(s) 488
TasI AATT 4 cut(s) 124, 186, 226, 275
TfiI GAWTC 2 cut(s) 18, 452
Tru1I TTAA 2 cut(s) 158, 278
Tru9I TTAA 2 cut(s) 158, 278
TscAI CASTG 2 cut(s) 28, 258
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 4 cut(s) 26, 215, 293, 563
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 476
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 2 cut(s) 28, 258
Vha464I CTTAAG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 2 cut(s) 186, 226
XspI CTAG 2 cut(s) 392, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.