FvH4_3g05490

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002223
Physical Location & Seq
Forward (+)
3213904 .. 3216099
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05490.t2

Sequence Viewer

Length: 1212 bp
ATGGCAATTTCTAGCAGAATTGCCAGAATCAACGCCTTTCTTCTCTGTTTGCTCTGTTTCTCAGCTTTACAAGGAGTTACATCACTTGGCATCAACTATGGCCAACTCGGCAACAATCTGCTAGAACCACAGAAAGTCCTAGACCTCTTAAGCTCCCTCAAGGTCACGAAAGCAAGAATTTACGACACGAATCCTCAAGTACTAACAGCATTTGCCAATTCCGGCGTTGAGCTAATGGTGACAATCGAAAACGCAATGTTAGGCCAGTTGATGAACCCCCAAGCAGCCCTTCAATGGGTTAACGCTCACATCAAGCCTTACGTCCCCGCAACGAAAATCACCAGCATTGCAGTAGGGAATGAGGTCTTCACCGACGATGACACAACCGTGCTGCTAGCTAATCTTGTCCCAGCCATGGTCGGCATTCAAACGGCGTTAACTCAGTTAGGCCTTGACTCATACATTAAGGTCTCAACACCTTGCTCTTTGGCAGTGCTTGAAGAATCCTATCCCCCTTCTGCAGGGAGCTTCAAGACTGAAGTAGCTGGTGTCATGTCACAATTGTTACAGTTCTTACAAACCACAAAGGCACCTTTTTGGATCAATGCCTATCCTTACTTTGCATACAAAGACAACCCGAATAAAATTTCTCTAGACTATGTGCTCTTCAATCCCAATTCTGGCATGATCGACCCTTACACCAAGTTACACTATGACAATATGTTGTATGCTCAAGTAGATGCAGCCATTTTCGCTATGGCGCGGTTGGGGTTCAATGGCATCGAGGTCAAGGTTTCGGAGACAGGGTGGCCATCCAAGGGCGACGCCAATGAAATCGGTGCAACTTTGCAGAACGCTGCTATTTATAATAGAAACTTGCTGAGGAGGCAATTGGCAAATGAAGGCACGCCTTTGAGGCCTAGAATGAGGCTGGAAGTCTATGTTTTTGCTCTTTTCAATGAGGACATGAAGCCTGGACCAACTTCAGAGAGGAATTATGGTTTATTTCAACCTGATGGAACTATGGCTTACAATGTGGGATTGTCTGCCTTATCAACTACTTCAACTACCTCATCAACCTCGACGGCTTCCATTTCTGATATCACTTCCTCTGCCACAAAGTCAACAACAATGGACTTTCATCAGAACTGGGTGAGCTGCATCTTTGTTTGTGTGGTAACCTTCCAAGTTTATATGAGACGACCATTCTAA

Protein Analysis

404

Amino Acids

44.22

Weight (kDa)

5.98

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 3 - 268 9.6e-66 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AccB1I GGYRCC 1 cut(s) 589
AccII CGCG 1 cut(s) 763
AciI CCGC 2 cut(s) 327, 763
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 2 cut(s) 100, 809
AcsI RAATTY 2 cut(s) 177, 645
AcuI CTGAAG 2 cut(s) 558, 969
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
AflII CTTAAG 1 cut(s) 148
AgsI TTSAA 9 cut(s) 293, 428, 500, 532, 670, 775, 958, 1010, 1065
AjnI CCWGG 1 cut(s) 973
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AleI CACNNNNGTG 1 cut(s) 386
AluBI AGCT 7 cut(s) 65, 153, 232, 398, 528, 545, 1158
AluI AGCT 7 cut(s) 65, 153, 232, 398, 528, 545, 1158
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 3 cut(s) 475, 794, 1192
AlwI GGATC 1 cut(s) 608
AoxI GGCC 5 cut(s) 100, 262, 448, 809, 917
ApeKI GCWGC 5 cut(s) 284, 391, 743, 857, 1158
ApoI RAATTY 2 cut(s) 177, 645
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 4 cut(s) 250, 331, 361, 1165
AsuNHI GCTAGC 1 cut(s) 394
AvaII GGWCC 1 cut(s) 977
BalI TGGCCA 2 cut(s) 102, 811
BanI GGYRCC 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 666
BbvCI CCTCAGC 1 cut(s) 881
BbvI GCAGC 5 cut(s) 296, 378, 755, 844, 1145
BccI CCATC 2 cut(s) 820, 1010
BceAI ACGGC 2 cut(s) 447, 1101
BciT130I CCWGG 1 cut(s) 975
BcoDI GTCTC 3 cut(s) 475, 794, 1192
BfaI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
BfmI CTRYAG 1 cut(s) 519
BfrI CTTAAG 1 cut(s) 148
BglI GCCNNNNNGGC 2 cut(s) 108, 916
BisI GCNGC 5 cut(s) 285, 392, 744, 858, 1159
BlsI GCNGC 5 cut(s) 286, 393, 745, 859, 1160
BmcAI AGTACT 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 975
Bme18I GGWCC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 1 cut(s) 975
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 4 cut(s) 99, 730, 789, 1170
BmtI GCTAGC 1 cut(s) 398
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 881
BpuEI CTTGAG 3 cut(s) 143, 180, 717
BsaHI GRCGYC 1 cut(s) 825
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 414, 816
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
Bse1I ACTGG 2 cut(s) 265, 1154
Bse3DI GCAATG 2 cut(s) 261, 345
BseBI CCWGG 1 cut(s) 975
BseDI CCNNGG 2 cut(s) 414, 816
BseGI GGATG 1 cut(s) 812
BseLI CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
BseMI GCAATG 2 cut(s) 261, 345
BseMII CTCAG 3 cut(s) 75, 455, 872
BseNI ACTGG 2 cut(s) 265, 1154
BseRI GAGGAG 1 cut(s) 898
BseXI GCAGC 5 cut(s) 296, 378, 755, 844, 1145
BseYI CCCAGC 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 763
BshFI GGCC 5 cut(s) 102, 264, 450, 811, 919
BshNI GGYRCC 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 666
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 2 cut(s) 308, 392
BslI CCNNNNNNNGG 6 cut(s) 294, 295, 415, 521, 680, 818
BsmAI GTCTC 3 cut(s) 475, 794, 1192
BsmBI CGTCTC 1 cut(s) 1192
BsmFI GGGAC 2 cut(s) 308, 392
BsmI GAATGC 1 cut(s) 423
BsnI GGCC 5 cut(s) 102, 264, 450, 811, 919
Bso31I GGTCTC 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 2 cut(s) 600, 687
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 2 cut(s) 327, 763
BspANI GGCC 5 cut(s) 102, 264, 450, 811, 919
BspCNI CTCAG 3 cut(s) 74, 454, 873
BspFNI CGCG 1 cut(s) 763
BspLI GGNNCC 1 cut(s) 591
BspMAI CTGCAG 1 cut(s) 523
BspOI GCTAGC 1 cut(s) 398
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 589
BspTI CTTAAG 1 cut(s) 148
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 2 cut(s) 261, 345
BsrI ACTGG 2 cut(s) 265, 1154
BssECI CCNNGG 2 cut(s) 414, 816
BssMI GATC 2 cut(s) 600, 687
BssNI GRCGYC 1 cut(s) 825
BssT1I CCWWGG 2 cut(s) 414, 816
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 2 cut(s) 388, 570
Bst6I CTCTTC 1 cut(s) 671
BstACI GRCGYC 1 cut(s) 825
BstAFI CTTAAG 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 396, 908
BstDEI CTNAG 3 cut(s) 61, 441, 881
BstDSI CCRYGG 1 cut(s) 414
BstEII GGTNACC 1 cut(s) 1177
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 812
BstFNI CGCG 1 cut(s) 763
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 2 cut(s) 603, 690
BstMAI GTCTC 3 cut(s) 475, 794, 1192
BstMBI GATC 2 cut(s) 600, 687
BstMWI GCNNNNNNNGC 4 cut(s) 108, 752, 886, 916
BstNI CCWGG 1 cut(s) 975
BstPI GGTNACC 1 cut(s) 1177
BstSCI CCNGG 1 cut(s) 973
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 1 cut(s) 763
BstV1I GCAGC 5 cut(s) 296, 378, 755, 844, 1145
BstV2I GAAGAC 1 cut(s) 358
BsuRI GGCC 5 cut(s) 102, 264, 450, 811, 919
BtgI CCRYGG 1 cut(s) 414
BtsCI GGATG 1 cut(s) 812
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 2 cut(s) 396, 908
CfoI GCGC 1 cut(s) 763
Cfr13I GGNCC 1 cut(s) 977
CseI GACGC 1 cut(s) 833
Csp6I GTAC 1 cut(s) 200
CviAII CATG 4 cut(s) 415, 553, 685, 967
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 61, 441, 881
DpnI GATC 2 cut(s) 602, 689
DpnII GATC 2 cut(s) 600, 687
EaeI YGGCCR 2 cut(s) 100, 809
Eam1104I CTCTTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 671
Eco130I CCWWGG 2 cut(s) 414, 816
Eco147I AGGCCT 2 cut(s) 450, 919
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 1 cut(s) 1102
Eco47I GGWCC 1 cut(s) 977
Eco57I CTGAAG 2 cut(s) 558, 969
Eco91I GGTNACC 1 cut(s) 1177
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoO65I GGTNACC 1 cut(s) 1177
EcoRII CCWGG 1 cut(s) 973
EcoRV GATATC 1 cut(s) 1102
EcoT14I CCWWGG 2 cut(s) 414, 816
ErhI CCWWGG 2 cut(s) 414, 816
Esp3I CGTCTC 1 cut(s) 1192
FaeI CATG 4 cut(s) 418, 556, 688, 970
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FaqI GGGAC 2 cut(s) 308, 392
FatI CATG 4 cut(s) 414, 552, 684, 966
FauI CCCGC 1 cut(s) 334
Fnu4HI GCNGC 5 cut(s) 285, 392, 744, 858, 1159
FokI GGATG 1 cut(s) 799
Fsp4HI GCNGC 5 cut(s) 285, 392, 744, 858, 1159
FspBI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
GlaI GCGC 1 cut(s) 762
GluI GCNGC 5 cut(s) 285, 392, 744, 858, 1159
GsaI CCCAGC 1 cut(s) 413
HaeIII GGCC 5 cut(s) 102, 264, 450, 811, 919
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 833
HhaI GCGC 1 cut(s) 763
Hin1I GRCGYC 1 cut(s) 825
Hin1II CATG 4 cut(s) 418, 556, 688, 970
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 3 cut(s) 301, 438, 1125
HindII GTYRAC 3 cut(s) 301, 438, 1125
HinfI GANTC 4 cut(s) 27, 190, 455, 503
HpaI GTTAAC 2 cut(s) 301, 438
HpaII CCGG 1 cut(s) 222
HphI GGTGA 4 cut(s) 250, 331, 361, 1165
Hpy166II GTNNAC 3 cut(s) 301, 438, 1125
Hpy188I TCNGA 4 cut(s) 799, 988, 1099, 1146
Hpy188III TCNNGA 3 cut(s) 166, 532, 653
Hpy8I GTNNAC 3 cut(s) 301, 438, 1125
Hpy99I CGWCG 3 cut(s) 377, 827, 1087
HpyAV CCTTC 4 cut(s) 299, 525, 896, 1192
HpyCH4III ACNGT 2 cut(s) 388, 570
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 7 cut(s) 350, 521, 623, 743, 842, 850, 1161
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 752, 886, 916
HpyF3I CTNAG 3 cut(s) 61, 441, 881
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 825
Hsp92II CATG 4 cut(s) 418, 556, 688, 970
HspAI GCGC 1 cut(s) 761
KspAI GTTAAC 2 cut(s) 301, 438
Kzo9I GATC 2 cut(s) 600, 687
LguI GCTCTTC 1 cut(s) 671
LmnI GCTCC 2 cut(s) 158, 525
Lsp1109I GCAGC 5 cut(s) 296, 378, 755, 844, 1145
LweI GCATC 4 cut(s) 99, 730, 789, 1170
MaeI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 7 cut(s) 76, 163, 238, 555, 564, 705, 1177
MalI GATC 2 cut(s) 602, 689
MboI GATC 2 cut(s) 600, 687
MboII GAAGA 4 cut(s) 32, 358, 512, 658
MfeI CAATTG 2 cut(s) 560, 890
MhlI GDGCHC 1 cut(s) 666
MlsI TGGCCA 2 cut(s) 102, 811
MluCI AATT 9 cut(s) 6, 18, 177, 217, 560, 645, 676, 890, 994
MluNI TGGCCA 2 cut(s) 102, 811
MlyI GAGTC 1 cut(s) 449
Mox20I TGGCCA 2 cut(s) 102, 811
MscI TGGCCA 2 cut(s) 102, 811
MseI TTAA 4 cut(s) 149, 300, 437, 465
MslI CAYNNNNRTG 1 cut(s) 386
Msp20I TGGCCA 2 cut(s) 102, 811
MspCI CTTAAG 1 cut(s) 148
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 975
MunI CAATTG 2 cut(s) 560, 890
Mva1269I GAATGC 1 cut(s) 423
MvaI CCWGG 1 cut(s) 975
MvnI CGCG 1 cut(s) 763
MwoI GCNNNNNNNGC 4 cut(s) 108, 752, 886, 916
NcoI CCATGG 1 cut(s) 414
NdeII GATC 2 cut(s) 600, 687
NheI GCTAGC 1 cut(s) 394
NlaIII CATG 4 cut(s) 418, 556, 688, 970
NlaIV GGNNCC 1 cut(s) 591
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 3 cut(s) 163, 238, 555
OliI CACNNNNGTG 1 cut(s) 386
PceI AGGCCT 2 cut(s) 450, 919
PciSI GCTCTTC 1 cut(s) 671
PctI GAATGC 1 cut(s) 423
PfeI GAWTC 3 cut(s) 27, 190, 503
PkrI GCNGC 5 cut(s) 286, 393, 745, 859, 1160
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PsiI TTATAA 1 cut(s) 867
Psp6I CCWGG 1 cut(s) 973
PspEI GGTNACC 1 cut(s) 1177
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 977
PstI CTGCAG 1 cut(s) 523
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 671
SaqAI TTAA 4 cut(s) 149, 300, 437, 465
SatI GCNGC 5 cut(s) 285, 392, 744, 858, 1159
Sau3AI GATC 2 cut(s) 600, 687
Sau96I GGNCC 1 cut(s) 977
ScaI AGTACT 1 cut(s) 201
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 975
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 4 cut(s) 99, 730, 789, 1170
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 977
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 4 cut(s) 148, 158, 195, 732
SmoI CTYRAG 4 cut(s) 148, 158, 195, 732
Sse9I AATT 9 cut(s) 6, 18, 177, 217, 560, 645, 676, 890, 994
SseBI AGGCCT 2 cut(s) 450, 919
SsiI CCGC 2 cut(s) 327, 763
SspMI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
StuI AGGCCT 2 cut(s) 450, 919
StyD4I CCNGG 1 cut(s) 973
StyI CCWWGG 2 cut(s) 414, 816
TaaI ACNGT 2 cut(s) 388, 570
TaiI ACGT 1 cut(s) 324
TaqI TCGA 4 cut(s) 246, 690, 783, 1082
TasI AATT 9 cut(s) 6, 18, 177, 217, 560, 645, 676, 890, 994
TatI WGTACW 1 cut(s) 199
TfiI GAWTC 3 cut(s) 27, 190, 503
Tru1I TTAA 4 cut(s) 149, 300, 437, 465
Tru9I TTAA 4 cut(s) 149, 300, 437, 465
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 3 cut(s) 163, 238, 555
TseI GCWGC 5 cut(s) 284, 391, 743, 857, 1158
Tsp45I GTSAC 3 cut(s) 163, 238, 555
TspDTI ATGAA 5 cut(s) 287, 846, 915, 983, 1130
TspRI CASTG 1 cut(s) 498
Vha464I CTTAAG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 977
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 2 cut(s) 177, 645
XbaI TCTAGA 1 cut(s) 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XspI CTAG 6 cut(s) 12, 122, 140, 395, 653, 921
ZrmI AGTACT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.