AT1G61070

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001620
Physical Location & Seq
Reverse (-)
22491692 .. 22492542
851 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61070.1

Sequence Viewer

Length: 231 bp
ATGAAGGTCTCTCCTCGTTTGAACTCAGCCCTTCTCCTATTGTTCATGATCCTTGCCACAGTGATGGGTCTGGTCACGGTAGAGGCACGGACGTGTGAAACAAGTAGCAACTTGTTCAACGGACCATGTCTGAGCTCAAGCAATTGCGCTAATGTTTGCCACAATGAAGGTTTTTCAGATGGTGACTGCCGTGGATTCCGTCGTCGCTGTTTGTGCACCAGACCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

76

Amino Acids

8.29

Weight (kDa)

8.52

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 30 - 76 3e-19 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 43
AflIII ACRYGT 1 cut(s) 92
AgsI TTSAA 2 cut(s) 22, 118
AjiI CACGTC 1 cut(s) 93
AleI CACNNNNGTG 1 cut(s) 91
AluBI AGCT 1 cut(s) 135
AluI AGCT 1 cut(s) 135
Alw21I GWGCWC 2 cut(s) 137, 218
Alw26I GTCTC 1 cut(s) 13
Alw44I GTGCAC 1 cut(s) 214
AlwI GGATC 1 cut(s) 43
ApaLI GTGCAC 1 cut(s) 214
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 137, 218
BccI CCATC 2 cut(s) 58, 173
BceAI ACGGC 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 39, 122
BseSI GKGCMC 1 cut(s) 218
BsiHKAI GWGCWC 2 cut(s) 137, 218
BsmAI GTCTC 1 cut(s) 13
Bso31I GGTCTC 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 137, 218
Bsp143I GATC 1 cut(s) 48
BspCNI CTCAG 2 cut(s) 38, 123
BspHI TCATGA 1 cut(s) 45
BspPI GGATC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 61, 79
BstDEI CTNAG 2 cut(s) 25, 131
BstDSI CCRYGG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 1 cut(s) 51
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 1 cut(s) 48
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 218
BstXI CCANNNNNNTGG 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 190
BtrI CACGTC 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 66
CciI TCATGA 1 cut(s) 45
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 122
CviAII CATG 2 cut(s) 46, 126
CviJI RGCY 2 cut(s) 29, 135
CviKI_1 RGCY 2 cut(s) 29, 135
DdeI CTNAG 2 cut(s) 25, 131
DpnI GATC 1 cut(s) 50
DpnII GATC 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 2 cut(s) 49, 129
FaiI YATR 2 cut(s) 47, 127
FatI CATG 2 cut(s) 45, 125
FriOI GRGCYC 1 cut(s) 137
GlaI GCGC 1 cut(s) 148
HhaI GCGC 1 cut(s) 149
Hin1II CATG 2 cut(s) 49, 129
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HinfI GANTC 1 cut(s) 195
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 2 cut(s) 132, 178
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 1 cut(s) 216
Hpy99I CGWCG 2 cut(s) 204, 207
HpyAV CCTTC 2 cut(s) 41, 161
HpyCH4III ACNGT 2 cut(s) 61, 79
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 2 cut(s) 25, 131
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 2 cut(s) 49, 129
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 1 cut(s) 48
LpnPI CCDG 1 cut(s) 56
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 2 cut(s) 73, 182
MalI GATC 1 cut(s) 50
MboI GATC 1 cut(s) 48
MfeI CAATTG 1 cut(s) 142
MhlI GDGCHC 2 cut(s) 137, 218
MluCI AATT 1 cut(s) 142
MnlI CCTC 2 cut(s) 24, 76
MslI CAYNNNNRTG 2 cut(s) 62, 91
MunI CAATTG 1 cut(s) 142
MwoI GCNNNNNNNGC 1 cut(s) 213
NdeII GATC 1 cut(s) 48
NlaIII CATG 2 cut(s) 49, 129
NmuCI GTSAC 2 cut(s) 73, 182
OliI CACNNNNGTG 1 cut(s) 91
PagI TCATGA 1 cut(s) 45
PfeI GAWTC 1 cut(s) 195
PflFI GACNNNGTC 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 62, 91
SacI GAGCTC 1 cut(s) 137
Sau3AI GATC 1 cut(s) 48
Sau96I GGNCC 1 cut(s) 122
SduI GDGCHC 2 cut(s) 137, 218
SetI ASST 5 cut(s) 9, 95, 137, 172, 226
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 2 cut(s) 62, 91
SmlI CTYRAG 1 cut(s) 136
SmoI CTYRAG 1 cut(s) 136
Sse9I AATT 1 cut(s) 142
SstI GAGCTC 1 cut(s) 137
TaaI ACNGT 2 cut(s) 61, 79
TaiI ACGT 1 cut(s) 95
TasI AATT 1 cut(s) 142
TfiI GAWTC 1 cut(s) 195
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 2 cut(s) 73, 182
Tsp45I GTSAC 2 cut(s) 73, 182
TspDTI ATGAA 3 cut(s) 17, 34, 180
TspGWI ACGGA 3 cut(s) 103, 135, 188
TspRI CASTG 1 cut(s) 66
Tth111I GACNNNGTC 1 cut(s) 126
VneI GTGCAC 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.