AT2G02100

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001620
Physical Location & Seq
Forward (+)
528179 .. 529186
1008 bp
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UTR
Exon/CDS
Intron
AT2G02100.1

Sequence Viewer

Length: 234 bp
ATGAAGCTCTCTATGCGTTTGATCTCAGCTGTTCTCATCATGTTCATGATATTCGTCGCCACTGGGATGGGACCAGTCACTGTGGAGGCACGCACTTGTGAGTCGCAGAGCCATAGGTTCAAGGGTACATGCGTGAGTGCATCAAACTGCGCCAACGTGTGCCACAACGAAGGCTTTGTCGGAGGTAACTGCCGTGGATTCCGTCGTCGTTGCTTCTGCACCAGACATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

77

Amino Acids

8.52

Weight (kDa)

9.37

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 31 - 77 1.2e-19 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 127
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 1 cut(s) 121
AluBI AGCT 2 cut(s) 7, 29
AluI AGCT 2 cut(s) 7, 29
AlwNI CAGNNNCTG 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 71
AvaII GGWCC 1 cut(s) 71
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 149
BmuI ACTGGG 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 67, 74
Bse3DI GCAATG 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 2 cut(s) 67, 74
BsgI GTGCAG 1 cut(s) 202
BslFI GGGAC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 21
BspCNI CTCAG 1 cut(s) 38
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 2 cut(s) 67, 74
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 72
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNSI RCATGY 1 cut(s) 132
BstXI CCANNNNNNTGG 1 cut(s) 67
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 60, 78
Cac8I GCNNGC 1 cut(s) 91
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 45
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 126
CviAII CATG 3 cut(s) 40, 46, 129
CviJI RGCY 4 cut(s) 7, 29, 111, 174
CviKI_1 RGCY 4 cut(s) 7, 29, 111, 174
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 1 cut(s) 25
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 71
FaeI CATG 3 cut(s) 43, 49, 132
FaiI YATR 5 cut(s) 14, 41, 47, 114, 130
FaqI GGGAC 1 cut(s) 84
FatI CATG 3 cut(s) 39, 45, 128
FokI GGATG 1 cut(s) 79
GlaI GCGC 1 cut(s) 151
HhaI GCGC 1 cut(s) 152
Hin1II CATG 3 cut(s) 43, 49, 132
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 2 cut(s) 101, 198
Hpy188I TCNGA 1 cut(s) 182
Hpy188III TCNNGA 1 cut(s) 46
Hpy99I CGWCG 3 cut(s) 59, 207, 210
HpyAV CCTTC 1 cut(s) 164
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 2 cut(s) 140, 219
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 25
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 3 cut(s) 43, 49, 132
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 1 cut(s) 21
LpnPI CCDG 2 cut(s) 48, 87
LweI GCATC 1 cut(s) 149
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 76, 185
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 2 cut(s) 79, 176
MslI CAYNNNNRTG 2 cut(s) 44, 65
MspA1I CMGCKG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 1 cut(s) 21
NlaIII CATG 3 cut(s) 43, 49, 132
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 132
PagI TCATGA 1 cut(s) 45
PfeI GAWTC 1 cut(s) 198
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 44, 65
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 110
SetI ASST 5 cut(s) 9, 31, 119, 159, 187
SfaNI GCATC 1 cut(s) 149
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 44, 65
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 159
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 2 cut(s) 67, 85
TseFI GTSAC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 17, 34
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 67, 85
VpaK11BI GGWCC 1 cut(s) 71
XceI RCATGY 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.