MD16G1009100.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001620
Physical Location & Seq
Reverse (-)
654987 .. 655990
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1009100.v1.1.491

Sequence Viewer

Length: 237 bp
ATGGAGCGTTCAATGCGTTTGTTTTCGACTGCCTTCGTCTTTGTTCTTCTTTTGGTGGCTACAGGGATGATGGGACCACTGCTTGCGGAGGGTAGGACCTGTGAGTCTCAGAGCCGCAAGTTCAAGGGAACCTGCCTGAGTAAAACCAACTGTGCATCTGTTTGCCAGACTGAGGGCTTCCCTGGAGGCAATTGTCGTGGCTTTCGCCGCAGATGCTTCTGCACCAAACATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

79

Amino Acids

8.65

Weight (kDa)

9.49

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 32 - 78 7.5e-19 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 140
AciI CCGC 3 cut(s) 86, 115, 208
AfiI CCNNNNNNNGG 1 cut(s) 172
AgsI TTSAA 2 cut(s) 12, 124
AjnI CCWGG 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 74, 96
AvaII GGWCC 2 cut(s) 74, 96
BccI CCATC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 140
BisI GCNGC 2 cut(s) 115, 208
BlsI GCNGC 2 cut(s) 116, 209
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 2 cut(s) 74, 96
BmgT120I GGNCC 2 cut(s) 74, 96
BmiI GGNNCC 2 cut(s) 75, 130
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 164, 203
BpmI CTGGAG 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 3 cut(s) 122, 128, 162
BsgI GTGCAG 1 cut(s) 205
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 87
BspACI CCGC 3 cut(s) 86, 115, 208
BspCNI CTCAG 3 cut(s) 121, 129, 163
BspLI GGNNCC 2 cut(s) 75, 130
BspMI ACCTGC 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 108, 137, 171
BstF5I GGATG 1 cut(s) 72
BstMAI GTCTC 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 13, 207, 213
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 72
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 77
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 74, 96
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviJI RGCY 4 cut(s) 59, 114, 177, 201
CviKI_1 RGCY 4 cut(s) 59, 114, 177, 201
DdeI CTNAG 3 cut(s) 108, 137, 171
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
Eco47I GGWCC 2 cut(s) 74, 96
EcoO109I RGGNCCY 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 181
FaqI GGGAC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 115, 208
FokI GGATG 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 115, 208
GluI GCNGC 2 cut(s) 115, 208
GsuI CTGGAG 1 cut(s) 204
HinfI GANTC 1 cut(s) 104
Hpy188I TCNGA 1 cut(s) 111
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 155, 222
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 207, 213
HpyF3I CTNAG 3 cut(s) 108, 137, 171
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 48, 112, 145, 149, 168, 179, 195
LweI GCATC 2 cut(s) 164, 203
MboII GAAGA 1 cut(s) 38
MfeI CAATTG 1 cut(s) 190
MluCI AATT 1 cut(s) 190
MlyI GAGTC 1 cut(s) 113
MnlI CCTC 3 cut(s) 82, 166, 179
MseI TTAA 1 cut(s) 235
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 13, 207, 213
NlaIV GGNNCC 2 cut(s) 75, 130
PkrI GCNGC 2 cut(s) 116, 209
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 75, 130
PspPI GGNCC 2 cut(s) 74, 96
PspPPI RGGWCCY 1 cut(s) 96
SaqAI TTAA 1 cut(s) 235
SatI GCNGC 2 cut(s) 115, 208
Sau96I GGNCC 2 cut(s) 74, 96
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 183
SetI ASST 2 cut(s) 101, 134
SfaNI GCATC 2 cut(s) 164, 203
SfcI CTRYAG 1 cut(s) 60
SinI GGWCC 2 cut(s) 74, 96
Sse9I AATT 1 cut(s) 190
SsiI CCGC 3 cut(s) 86, 115, 208
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 26
TasI AATT 1 cut(s) 190
TauI GCSGC 2 cut(s) 117, 210
Tru1I TTAA 1 cut(s) 235
Tru9I TTAA 1 cut(s) 235
TscAI CASTG 1 cut(s) 84
TspRI CASTG 1 cut(s) 84
VpaK11BI GGWCC 2 cut(s) 74, 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.