MD16G1009000.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001620
Physical Location & Seq
Reverse (-)
653133 .. 654077
945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1009000.v1.1.491

Sequence Viewer

Length: 228 bp
ATGGAGCGTTCCATGCGTTTATTTCCAACCGTCTTCGTCTTCCTGCTACTTTTGGTGGCCACCGGGACGATGGTTGCTGAGGGTAGGACCTGCGAGTCTCAGAGTAACCGTTTCAAAGGAAGCTGCGTGAGTAAAAGTAACTGTGCTGCTGTTTGCCAAACTGAGGGCTTCCCCGGGGGCAATTGCCGTGGCTTACGCCGTAGATGCTTTTGCACTAAACATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

76

Amino Acids

8.29

Weight (kDa)

9.33

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 29 - 75 7.5e-19 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 94
Acc36I ACCTGC 1 cut(s) 98
AcoI YGGCCR 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 1 cut(s) 115
AluBI AGCT 1 cut(s) 123
AluI AGCT 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 123, 146
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 3 cut(s) 64, 174, 175
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 1 cut(s) 87
BalI TGGCCA 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 25, 31
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 110, 133
BccI CCATC 1 cut(s) 64
BceAI ACGGC 2 cut(s) 171, 183
BcnI CCSGG 3 cut(s) 64, 174, 175
BcoDI GTCTC 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 98
BisI GCNGC 2 cut(s) 124, 147
BlsI GCNGC 2 cut(s) 125, 148
Bme1390I CCNGG 3 cut(s) 64, 174, 175
Bme18I GGWCC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 87
BmrFI CCNGG 3 cut(s) 64, 174, 175
BmsI GCATC 1 cut(s) 194
BpiI GAAGAC 2 cut(s) 25, 31
Bpu10I CCTNAGC 1 cut(s) 78
BpuMI CCSGG 3 cut(s) 64, 174, 175
BsaJI CCNNGG 4 cut(s) 172, 173, 174, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseDI CCNNGG 4 cut(s) 172, 173, 174, 187
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 3 cut(s) 69, 113, 153
BseXI GCAGC 2 cut(s) 110, 133
BshFI GGCC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 63, 174
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 173
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 3 cut(s) 70, 112, 154
BspMI ACCTGC 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 172, 173, 174, 187
Bst4CI ACNGT 3 cut(s) 31, 110, 143
BstDEI CTNAG 3 cut(s) 78, 99, 162
BstDSI CCRYGG 1 cut(s) 187
BstMAI GTCTC 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 13, 204
BstSCI CCNGG 3 cut(s) 62, 172, 173
BstV1I GCAGC 2 cut(s) 110, 133
BstV2I GAAGAC 2 cut(s) 25, 31
BsuRI GGCC 1 cut(s) 59
BtgI CCRYGG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 87
Cfr9I CCCGGG 1 cut(s) 173
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 1 cut(s) 13
CviJI RGCY 4 cut(s) 59, 123, 168, 192
CviKI_1 RGCY 4 cut(s) 59, 123, 168, 192
DdeI CTNAG 3 cut(s) 78, 99, 162
DrdI GACNNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 87
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FaqI GGGAC 1 cut(s) 79
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 124, 147
Fsp4HI GCNGC 2 cut(s) 124, 147
GluI GCNGC 2 cut(s) 124, 147
HaeIII GGCC 1 cut(s) 59
HapII CCGG 2 cut(s) 63, 174
Hin1II CATG 1 cut(s) 16
HinfI GANTC 1 cut(s) 95
HpaII CCGG 2 cut(s) 63, 174
Hpy188I TCNGA 1 cut(s) 102
HpyCH4III ACNGT 3 cut(s) 31, 110, 143
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 204
HpyF3I CTNAG 3 cut(s) 78, 99, 162
Hsp92II CATG 1 cut(s) 16
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 56, 76, 103, 187
Lsp1109I GCAGC 2 cut(s) 110, 133
LweI GCATC 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 104, 137
MboII GAAGA 2 cut(s) 25, 31
MfeI CAATTG 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 1 cut(s) 181
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 2 cut(s) 73, 157
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 1 cut(s) 226
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 2 cut(s) 63, 174
MspR9I CCNGG 3 cut(s) 64, 174, 175
MunI CAATTG 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 13, 204
NciI CCSGG 3 cut(s) 64, 174, 175
NlaIII CATG 1 cut(s) 16
PkrI GCNGC 2 cut(s) 125, 148
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 87
PspPI GGNCC 1 cut(s) 87
PspPPI RGGWCCY 1 cut(s) 87
SaqAI TTAA 1 cut(s) 226
SatI GCNGC 2 cut(s) 124, 147
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 64, 174, 175
SetI ASST 2 cut(s) 92, 125
SfaNI GCATC 1 cut(s) 194
SinI GGWCC 1 cut(s) 87
SmaI CCCGGG 1 cut(s) 175
Sse9I AATT 1 cut(s) 181
StyD4I CCNGG 3 cut(s) 62, 172, 173
TaaI ACNGT 3 cut(s) 31, 110, 143
TasI AATT 1 cut(s) 181
Tru1I TTAA 1 cut(s) 226
Tru9I TTAA 1 cut(s) 226
TseI GCWGC 2 cut(s) 123, 146
TspMI CCCGGG 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 87
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmaI CCCGGG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.