FvH4_4g36102

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001620
Physical Location & Seq
Forward (+)
33203932 .. 33204282
351 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36102.t1

Sequence Viewer

Length: 240 bp
ATGGAGAGTTTCATGCGTATGTTTTCGGCTGTCTTCGTCGCTCTTCTGCTTCTGGTGGCTACTGGAATGGGGCCAAATGCAATGGTTGCAGAAGCAAGAACTTGTGAGACTTTGAGCCACAAGTTCAAGGGAACTTGCTTGAGAGAAAGCAACTGTGCATCTGTTTGCCAAACTGAGGGCTTCACCGGAGGCGACTGCCGCGGTCTTCGCCGCAGATGCTTCTGCACCAAACACTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

80

Amino Acids

8.68

Weight (kDa)

8.75

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 33 - 79 1.8e-18 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 199, 201, 211
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 101
AoxI GGCC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 175
BbsI GAAGAC 2 cut(s) 25, 197
BcoDI GTCTC 1 cut(s) 101
BisI GCNGC 2 cut(s) 199, 211
BlsI GCNGC 2 cut(s) 200, 212
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 2 cut(s) 167, 206
BpiI GAAGAC 2 cut(s) 25, 197
BpuEI CTTGAG 1 cut(s) 160
BsaJI CCNNGG 1 cut(s) 199
BsaWI WCCGGW 1 cut(s) 185
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 208
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 101
BsnI GGCC 1 cut(s) 73
BspACI CCGC 3 cut(s) 199, 201, 211
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 72
BspQI GCTCTTC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 87
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 155, 236
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 86
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 201
BstMAI GTCTC 1 cut(s) 101
BstMWI GCNNNNNNNGC 4 cut(s) 86, 198, 207, 216
BstUI CGCG 1 cut(s) 201
BstV2I GAAGAC 2 cut(s) 25, 197
BsuRI GGCC 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 71
Cfr42I CCGCGG 1 cut(s) 202
CviAII CATG 1 cut(s) 13
CviJI RGCY 5 cut(s) 29, 59, 73, 117, 180
CviKI_1 RGCY 5 cut(s) 29, 59, 73, 117, 180
DdeI CTNAG 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
FaeI CATG 1 cut(s) 16
FaiI YATR 2 cut(s) 14, 20
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 199, 211
Fsp4HI GCNGC 2 cut(s) 199, 211
GluI GCNGC 2 cut(s) 199, 211
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 186
Hin1II CATG 1 cut(s) 16
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 2 cut(s) 155, 236
HpyCH4V TGCA 4 cut(s) 80, 89, 158, 225
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 198, 207, 216
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 1 cut(s) 16
KspI CCGCGG 1 cut(s) 202
LguI GCTCTTC 1 cut(s) 48
LpnPI CCDG 3 cut(s) 38, 48, 199
LweI GCATC 2 cut(s) 167, 206
MboII GAAGA 3 cut(s) 25, 35, 197
MnlI CCTC 2 cut(s) 169, 182
MseI TTAA 1 cut(s) 238
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 86, 198, 207, 216
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 48
PkrI GCNGC 2 cut(s) 200, 212
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 17
SacII CCGCGG 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 238
SatI GCNGC 2 cut(s) 199, 211
Sau96I GGNCC 1 cut(s) 71
SfaNI GCATC 2 cut(s) 167, 206
Sfr303I CCGCGG 1 cut(s) 202
SgrBI CCGCGG 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SsiI CCGC 3 cut(s) 199, 201, 211
TaaI ACNGT 2 cut(s) 155, 236
TauI GCSGC 2 cut(s) 201, 213
Tru1I TTAA 1 cut(s) 238
Tru9I TTAA 1 cut(s) 238
TscAI CASTG 1 cut(s) 239
TspRI CASTG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.