MD02G1301100.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001620
Physical Location & Seq
Reverse (-)
35577768 .. 35578534
767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1301100.v1.1.491

Sequence Viewer

Length: 237 bp
ATGGAGCGTTCCATGCGTTTGTTTTCGACTGCCTTCGTCTTTGTTCTTCTTTTGGTGGCTACAGGGATGATGGGACCAATGCTTGCGGAGGGTAGGACCTGTGAGTCTCACAGCCGCAAGTACAAGGGAACCTGCCTGGGTAAAAGCAACTGTGCATCTGTTTGCCAGACTGAGGGCTTCCATGGAGGCCATTGTCGTGGCTTTCGCCGCAGATGCTTCTGCACTAAACATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

79

Amino Acids

8.71

Weight (kDa)

9.45

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 32 - 78 5.5e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 140
AciI CCGC 3 cut(s) 86, 115, 208
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 172
AjnI CCWGG 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 111
AoxI GGCC 1 cut(s) 187
AspS9I GGNCC 2 cut(s) 74, 96
AvaII GGWCC 2 cut(s) 74, 96
BccI CCATC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 140
BisI GCNGC 2 cut(s) 115, 208
BlsI GCNGC 2 cut(s) 116, 209
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 2 cut(s) 74, 96
BmgT120I GGNCC 2 cut(s) 74, 96
BmiI GGNNCC 2 cut(s) 75, 130
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 164, 203
BsaJI CCNNGG 2 cut(s) 136, 181
Bsc4I CCNNNNNNNGG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 136, 181
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 162
BsgI GTGCAG 1 cut(s) 205
BshFI GGCC 1 cut(s) 189
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 1 cut(s) 189
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 3 cut(s) 86, 115, 208
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 75, 130
BspMI ACCTGC 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 136, 181
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 171
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 72
BstMAI GTCTC 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 13, 207, 213
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 197
BsuRI GGCC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 72
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 74, 96
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 13, 182
CviJI RGCY 5 cut(s) 59, 114, 177, 189, 201
CviKI_1 RGCY 5 cut(s) 59, 114, 177, 189, 201
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 1 cut(s) 171
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 74, 96
EcoO109I RGGNCCY 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 2 cut(s) 16, 185
FaiI YATR 2 cut(s) 14, 183
FaqI GGGAC 1 cut(s) 87
FatI CATG 2 cut(s) 12, 181
Fnu4HI GCNGC 2 cut(s) 115, 208
FokI GGATG 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 115, 208
GluI GCNGC 2 cut(s) 115, 208
HaeIII GGCC 1 cut(s) 189
Hin1II CATG 2 cut(s) 16, 185
HinfI GANTC 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 155, 222
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 207, 213
HpyF3I CTNAG 1 cut(s) 171
Hsp92II CATG 2 cut(s) 16, 185
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 6 cut(s) 48, 112, 122, 145, 149, 179
LweI GCATC 2 cut(s) 164, 203
MboII GAAGA 1 cut(s) 38
MlyI GAGTC 1 cut(s) 113
MnlI CCTC 3 cut(s) 82, 166, 179
MseI TTAA 1 cut(s) 235
MslI CAYNNNNRTG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 13, 207, 213
NcoI CCATGG 1 cut(s) 181
NlaIII CATG 2 cut(s) 16, 185
NlaIV GGNNCC 2 cut(s) 75, 130
PkrI GCNGC 2 cut(s) 116, 209
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 75, 130
PspPI GGNCC 2 cut(s) 74, 96
PspPPI RGGWCCY 1 cut(s) 96
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 1 cut(s) 235
SatI GCNGC 2 cut(s) 115, 208
Sau96I GGNCC 2 cut(s) 74, 96
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 137
SetI ASST 2 cut(s) 101, 134
SfaNI GCATC 2 cut(s) 164, 203
SfcI CTRYAG 1 cut(s) 60
SinI GGWCC 2 cut(s) 74, 96
SmiMI CAYNNNNRTG 1 cut(s) 195
SsiI CCGC 3 cut(s) 86, 115, 208
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 26
TatI WGTACW 1 cut(s) 120
TauI GCSGC 2 cut(s) 117, 210
Tru1I TTAA 1 cut(s) 235
Tru9I TTAA 1 cut(s) 235
VpaK11BI GGWCC 2 cut(s) 74, 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.