pycom02g25280

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001620
Physical Location & Seq
Reverse (-)
23296049 .. 23296385
337 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25280.1

Sequence Viewer

Length: 228 bp
ATGGAGCGTACCATGCGTTTATTTCCAACCGCCTTCGTCTTCCTGCTGCTTTTGGTGGCCACCGGGACGATGGTTGCTGAGGGTAGGACCTGCGAGTCTCAGAGTAACCGCTTCAAAGGAACCTGCGCGAGTAAAAGTAACTGTGCTGCTGTTTGCCAAACTGAGGGCTTCCCCGGAGGCAATTGTCGTGGCTTACGCCGTAGATGCTTTTGCACTAAACATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

76

Amino Acids

8.26

Weight (kDa)

9.33

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 29 - 75 7.3e-19 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 94
Acc36I ACCTGC 2 cut(s) 98, 131
AccII CGCG 1 cut(s) 128
AciI CCGC 2 cut(s) 30, 109
AcoI YGGCCR 1 cut(s) 57
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 102
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 46, 146
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 2 cut(s) 64, 174
AvaII GGWCC 1 cut(s) 87
BalI TGGCCA 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 33, 133
BccI CCATC 1 cut(s) 64
BceAI ACGGC 1 cut(s) 183
BcnI CCSGG 2 cut(s) 64, 174
BcoDI GTCTC 1 cut(s) 102
BfuAI ACCTGC 2 cut(s) 98, 131
BisI GCNGC 2 cut(s) 47, 147
BlsI GCNGC 2 cut(s) 48, 148
Bme1390I CCNGG 2 cut(s) 64, 174
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 2 cut(s) 64, 174
BmsI GCATC 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 78
BpuMI CCSGG 2 cut(s) 64, 174
BsaJI CCNNGG 1 cut(s) 172
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 3 cut(s) 69, 113, 153
BseXI GCAGC 2 cut(s) 33, 133
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 59
BsiSI CCGG 2 cut(s) 63, 174
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 1 cut(s) 59
BspACI CCGC 2 cut(s) 30, 109
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 3 cut(s) 70, 112, 154
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 1 cut(s) 121
BspMI ACCTGC 2 cut(s) 98, 131
BssECI CCNNGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 143
BstDEI CTNAG 3 cut(s) 78, 99, 162
BstFNI CGCG 1 cut(s) 128
BstHHI GCGC 1 cut(s) 128
BstMAI GTCTC 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 13, 204
BstSCI CCNGG 2 cut(s) 62, 172
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 2 cut(s) 33, 133
BstV2I GAAGAC 1 cut(s) 31
BsuRI GGCC 1 cut(s) 59
BveI ACCTGC 2 cut(s) 98, 131
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 1 cut(s) 9
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 1 cut(s) 13
CviJI RGCY 3 cut(s) 59, 168, 192
CviKI_1 RGCY 3 cut(s) 59, 168, 192
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 3 cut(s) 78, 99, 162
DrdI GACNNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 87
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FaqI GGGAC 1 cut(s) 79
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 47, 147
Fsp4HI GCNGC 2 cut(s) 47, 147
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 47, 147
HaeIII GGCC 1 cut(s) 59
HapII CCGG 2 cut(s) 63, 174
HhaI GCGC 1 cut(s) 128
Hin1II CATG 1 cut(s) 16
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 1 cut(s) 95
HpaII CCGG 2 cut(s) 63, 174
Hpy188I TCNGA 1 cut(s) 102
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 204
HpyF3I CTNAG 3 cut(s) 78, 99, 162
Hsp92II CATG 1 cut(s) 16
HspAI GCGC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 56, 76, 103, 136, 187
Lsp1109I GCAGC 2 cut(s) 33, 133
LweI GCATC 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 104, 137
MboII GAAGA 1 cut(s) 31
MfeI CAATTG 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 1 cut(s) 181
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 3 cut(s) 73, 157, 170
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 1 cut(s) 226
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 2 cut(s) 63, 174
MspR9I CCNGG 2 cut(s) 64, 174
MunI CAATTG 1 cut(s) 181
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 13, 204
NciI CCSGG 2 cut(s) 64, 174
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 1 cut(s) 121
PkrI GCNGC 2 cut(s) 48, 148
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 87
PspPPI RGGWCCY 1 cut(s) 87
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
SaqAI TTAA 1 cut(s) 226
SatI GCNGC 2 cut(s) 47, 147
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 64, 174
SetI ASST 2 cut(s) 92, 125
SfaNI GCATC 1 cut(s) 194
SinI GGWCC 1 cut(s) 87
Sse9I AATT 1 cut(s) 181
SsiI CCGC 2 cut(s) 30, 109
StyD4I CCNGG 2 cut(s) 62, 172
TaaI ACNGT 1 cut(s) 143
TasI AATT 1 cut(s) 181
Tru1I TTAA 1 cut(s) 226
Tru9I TTAA 1 cut(s) 226
TseI GCWGC 2 cut(s) 46, 146
VpaK11BI GGWCC 1 cut(s) 87
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.