Rroxscaffold_5G00386270

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001620
Physical Location & Seq
Forward (+)
65200194 .. 65200844
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386270.1

Sequence Viewer

Length: 243 bp
ATGGAAGGTTTCATGCGTTTGTTTTCGACTTTCTTCGTCGGTCTCCTGCTTCTGGTGGCTACAGCTGGGATAGGGCCAAATGTAATGGTTGCTGAGGCCAGAAAATGTGAGAGTCAGAGCCACAAGTTCGAGGGAATTTGCTTGAAACAGAACAATTGTGCATCTATTTGCAAAACTGAGGGTTACAGTGGAGGCAACTGCCGCGGTTTTCGCCGCAGATGCTTCTGCACCAAAGATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

80

Amino Acids

8.84

Weight (kDa)

8.9

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 34 - 80 6.5e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 204
AciI CCGC 3 cut(s) 202, 204, 214
AcsI RAATTY 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 1 cut(s) 145
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 47
AoxI GGCC 2 cut(s) 74, 96
ApoI RAATTY 1 cut(s) 135
AspS9I GGNCC 1 cut(s) 74
BbvCI CCTCAGC 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 47
BfmI CTRYAG 1 cut(s) 60
BisI GCNGC 2 cut(s) 202, 214
BlsI GCNGC 2 cut(s) 203, 215
BmgT120I GGNCC 1 cut(s) 74
BmsI GCATC 2 cut(s) 170, 209
Bpu10I CCTNAGC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 52
BseDI CCNNGG 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMII CTCAG 2 cut(s) 84, 168
BseYI CCCAGC 1 cut(s) 65
BsgI GTGCAG 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 76, 98
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 1 cut(s) 47
BsnI GGCC 2 cut(s) 76, 98
Bso31I GGTCTC 1 cut(s) 47
BspACI CCGC 3 cut(s) 202, 204, 214
BspANI GGCC 2 cut(s) 76, 98
BspCNI CTCAG 2 cut(s) 85, 169
BspFNI CGCG 1 cut(s) 204
BspTNI GGTCTC 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 202
Bst4CI ACNGT 1 cut(s) 188
BstDEI CTNAG 2 cut(s) 93, 177
BstDSI CCRYGG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 204
BstMAI GTCTC 1 cut(s) 47
BstMWI GCNNNNNNNGC 3 cut(s) 201, 210, 219
BstSFI CTRYAG 1 cut(s) 60
BstUI CGCG 1 cut(s) 204
BsuRI GGCC 2 cut(s) 76, 98
BtgI CCRYGG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 74
Cfr42I CCGCGG 1 cut(s) 205
CviAII CATG 1 cut(s) 13
CviJI RGCY 5 cut(s) 59, 65, 76, 98, 120
CviKI_1 RGCY 5 cut(s) 59, 65, 76, 98, 120
DdeI CTNAG 2 cut(s) 93, 177
Eco31I GGTCTC 1 cut(s) 47
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 202, 214
Fsp4HI GCNGC 2 cut(s) 202, 214
GluI GCNGC 2 cut(s) 202, 214
GsaI CCCAGC 1 cut(s) 69
HaeIII GGCC 2 cut(s) 76, 98
Hin1II CATG 1 cut(s) 16
HinfI GANTC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 117
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4V TGCA 3 cut(s) 161, 171, 228
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 210, 219
HpyF3I CTNAG 2 cut(s) 93, 177
Hsp92II CATG 1 cut(s) 16
KspI CCGCGG 1 cut(s) 205
LpnPI CCDG 4 cut(s) 38, 51, 59, 112
LweI GCATC 2 cut(s) 170, 209
MaeIII GTNAC 1 cut(s) 182
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 154
MluCI AATT 2 cut(s) 135, 154
MlyI GAGTC 1 cut(s) 121
MnlI CCTC 4 cut(s) 88, 124, 172, 185
MseI TTAA 1 cut(s) 241
MspA1I CMGCKG 2 cut(s) 65, 204
MunI CAATTG 1 cut(s) 154
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 3 cut(s) 201, 210, 219
NlaIII CATG 1 cut(s) 16
PkrI GCNGC 2 cut(s) 203, 215
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PspFI CCCAGC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 65
SacII CCGCGG 1 cut(s) 205
SaqAI TTAA 1 cut(s) 241
SatI GCNGC 2 cut(s) 202, 214
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 1 cut(s) 121
SetI ASST 2 cut(s) 10, 67
SfaNI GCATC 2 cut(s) 170, 209
SfcI CTRYAG 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 205
SgeI CNNG 9 cut(s) 25, 58, 65, 78, 111, 136, 142, 154, 215
SgrBI CCGCGG 1 cut(s) 205
Sse9I AATT 2 cut(s) 135, 154
SsiI CCGC 3 cut(s) 202, 204, 214
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 2 cut(s) 26, 129
TaqII GACCGA 1 cut(s) 29
TasI AATT 2 cut(s) 135, 154
TauI GCSGC 2 cut(s) 204, 216
Tru1I TTAA 1 cut(s) 241
Tru9I TTAA 1 cut(s) 241
TscAI CASTG 1 cut(s) 193
TspRI CASTG 1 cut(s) 193
XapI RAATTY 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.