Rw4G036510

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001620
Physical Location & Seq
Forward (+)
63333797 .. 63334561
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036510.1

Sequence Viewer

Length: 240 bp
ATGGAGGGTTTCATGCGTATGTTTTCAACTTTCTTCGTCGCTATCCTGCTCCTGGTGGCTACTGGGATGGGGCCGAATGCAATGGTCGCTGAGGCCAGAACTTGTGAGACTCTGAGCCACAAGTTCAAGGGAACTTGCTTGAGAGAGAGCAACTGCGCATCTGTTTGCCAAACTGAGGGCTTCAGTGGAGGTGACTGCCGTGGTCTTCGCCGCAGATGCTTCTGCACCAAACATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

79

Amino Acids

8.71

Weight (kDa)

8.75

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 33 - 79 2e-18 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 157
AciI CCGC 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 166
AfiI CCNNNNNNNGG 2 cut(s) 52, 175
AgsI TTSAA 2 cut(s) 27, 127
AjnI CCWGG 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 101
AoxI GGCC 2 cut(s) 71, 93
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 197
BbvCI CCTCAGC 1 cut(s) 90
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 101
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 2 cut(s) 167, 206
BmuI ACTGGG 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 160
BsaJI CCNNGG 1 cut(s) 199
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 175
Bse1I ACTGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 199
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 52, 175
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 3 cut(s) 81, 104, 165
BseNI ACTGG 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 208
BshFI GGCC 2 cut(s) 73, 95
BslI CCNNNNNNNGG 2 cut(s) 52, 175
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 2 cut(s) 73, 95
BspACI CCGC 1 cut(s) 211
BspANI GGCC 2 cut(s) 73, 95
BspCNI CTCAG 3 cut(s) 82, 105, 166
BspLI GGNNCC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 87
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 53
BstDEI CTNAG 3 cut(s) 90, 113, 174
BstDSI CCRYGG 1 cut(s) 199
BstF5I GGATG 1 cut(s) 72
BstHHI GCGC 1 cut(s) 158
BstMAI GTCTC 1 cut(s) 101
BstMWI GCNNNNNNNGC 2 cut(s) 86, 216
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstV2I GAAGAC 1 cut(s) 197
BsuRI GGCC 2 cut(s) 73, 95
BtgI CCRYGG 1 cut(s) 199
BtsCI GGATG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 190
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 71
CviAII CATG 1 cut(s) 13
CviJI RGCY 5 cut(s) 59, 73, 95, 117, 180
CviKI_1 RGCY 5 cut(s) 59, 73, 95, 117, 180
DdeI CTNAG 3 cut(s) 90, 113, 174
Eco57I CTGAAG 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 51
FaeI CATG 1 cut(s) 16
FaiI YATR 2 cut(s) 14, 20
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 1 cut(s) 79
Fsp4HI GCNGC 1 cut(s) 211
FspI TGCGCA 1 cut(s) 157
GlaI GCGC 1 cut(s) 157
GluI GCNGC 1 cut(s) 211
HaeIII GGCC 2 cut(s) 73, 95
HhaI GCGC 1 cut(s) 158
Hin1II CATG 1 cut(s) 16
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HinfI GANTC 1 cut(s) 109
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 1 cut(s) 114
Hpy99I CGWCG 1 cut(s) 41
HpyCH4V TGCA 2 cut(s) 80, 225
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 216
HpyF3I CTNAG 3 cut(s) 90, 113, 174
Hsp92II CATG 1 cut(s) 16
HspAI GCGC 1 cut(s) 156
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 5 cut(s) 38, 48, 59, 65, 109
LweI GCATC 2 cut(s) 167, 206
MaeIII GTNAC 1 cut(s) 191
MboII GAAGA 2 cut(s) 25, 197
MlyI GAGTC 1 cut(s) 103
MnlI CCTC 3 cut(s) 85, 169, 182
MseI TTAA 1 cut(s) 238
MslI CAYNNNNRTG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 86, 216
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 191
NsbI TGCGCA 1 cut(s) 157
PctI GAATGC 1 cut(s) 82
PkrI GCNGC 1 cut(s) 212
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 238
SatI GCNGC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 1 cut(s) 53
SetI ASST 1 cut(s) 193
SfaNI GCATC 2 cut(s) 167, 206
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SsiI CCGC 1 cut(s) 211
StyD4I CCNGG 1 cut(s) 51
TauI GCSGC 1 cut(s) 213
Tru1I TTAA 1 cut(s) 238
Tru9I TTAA 1 cut(s) 238
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspRI CASTG 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.