MD13G1012400.v1.1

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001620
Physical Location & Seq
Reverse (-)
718066 .. 718844
779 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1012400.v1.1.491

Sequence Viewer

Length: 234 bp
ATGGAGCGGTCCATGCGTTTATTTTCAACTGCCTTCCTCTTCCTTCTTTTGGTGGCTACCGGGATGATGGGACCAATGGTTGCAGAGGGTAGGACCTGTGACTCTCAGAGCCACCGGTTCAAGGGAAGCTGCGTGAGTAAAAGTAACTGTGCAACTGTTTGCCAGACTGAGGGCTTCCGTGGAGGCCATTGTCGTGGCTTTCGCCGCAGATGCTTCTGCACTAAACATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

78

Amino Acids

8.62

Weight (kDa)

9.54

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 31 - 77 1.7e-18 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 7
AciI CCGC 2 cut(s) 7, 205
AfiI CCNNNNNNNGG 3 cut(s) 49, 121, 169
AgeI ACCGGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 27, 121
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 129
AsiGI ACCGGT 1 cut(s) 114
AspS9I GGNCC 3 cut(s) 9, 71, 93
AsuC2I CCSGG 1 cut(s) 61
AvaII GGWCC 3 cut(s) 9, 71, 93
BbvI GCAGC 1 cut(s) 116
BccI CCATC 1 cut(s) 61
BcnI CCSGG 1 cut(s) 61
BisI GCNGC 2 cut(s) 130, 205
BlsI GCNGC 2 cut(s) 131, 206
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 3 cut(s) 9, 71, 93
BmgT120I GGNCC 3 cut(s) 9, 71, 93
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 121, 169
Bse118I RCCGGY 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 1 cut(s) 69
BseLI CCNNNNNNNGG 3 cut(s) 49, 121, 169
BseMII CTCAG 2 cut(s) 119, 159
BseXI GCAGC 1 cut(s) 116
BsgI GTGCAG 1 cut(s) 202
BshFI GGCC 1 cut(s) 186
BshTI ACCGGT 1 cut(s) 114
BsiSI CCGG 2 cut(s) 60, 115
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 49, 121, 169
BsmFI GGGAC 1 cut(s) 84
BsnI GGCC 1 cut(s) 186
BspACI CCGC 2 cut(s) 7, 205
BspANI GGCC 1 cut(s) 186
BspCNI CTCAG 2 cut(s) 118, 160
BspLI GGNNCC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 178
Bst4CI ACNGT 2 cut(s) 149, 157
Bst6I CTCTTC 1 cut(s) 44
BstDEI CTNAG 2 cut(s) 105, 168
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 1 cut(s) 69
BstMWI GCNNNNNNNGC 3 cut(s) 13, 204, 210
BstSCI CCNGG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 194
BsuRI GGCC 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 1 cut(s) 69
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 3 cut(s) 9, 71, 93
CspAI ACCGGT 1 cut(s) 114
CviAII CATG 1 cut(s) 13
CviJI RGCY 6 cut(s) 56, 111, 129, 174, 186, 198
CviKI_1 RGCY 6 cut(s) 56, 111, 129, 174, 186, 198
DdeI CTNAG 2 cut(s) 105, 168
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco47I GGWCC 3 cut(s) 9, 71, 93
EcoO109I RGGNCCY 1 cut(s) 93
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FaqI GGGAC 1 cut(s) 84
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 130, 205
FokI GGATG 1 cut(s) 76
Fsp4HI GCNGC 2 cut(s) 130, 205
GluI GCNGC 2 cut(s) 130, 205
HaeIII GGCC 1 cut(s) 186
HapII CCGG 2 cut(s) 60, 115
Hin1II CATG 1 cut(s) 16
HinfI GANTC 1 cut(s) 101
HpaII CCGG 2 cut(s) 60, 115
Hpy188I TCNGA 1 cut(s) 108
HpyAV CCTTC 2 cut(s) 43, 53
HpyCH4III ACNGT 2 cut(s) 149, 157
HpyCH4V TGCA 3 cut(s) 83, 152, 219
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 204, 210
HpyF3I CTNAG 2 cut(s) 105, 168
Hsp92II CATG 1 cut(s) 16
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 73, 109, 128, 176
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 98, 143
MbiI CCGCTC 1 cut(s) 7
MboII GAAGA 1 cut(s) 31
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 4 cut(s) 47, 79, 163, 176
MseI TTAA 1 cut(s) 232
MslI CAYNNNNRTG 1 cut(s) 192
MspI CCGG 2 cut(s) 60, 115
MspR9I CCNGG 1 cut(s) 61
MwoI GCNNNNNNNGC 3 cut(s) 13, 204, 210
NciI CCSGG 1 cut(s) 61
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 114
PkrI GCNGC 2 cut(s) 131, 206
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 3 cut(s) 9, 71, 93
PspPPI RGGWCCY 1 cut(s) 93
RseI CAYNNNNRTG 1 cut(s) 192
SaqAI TTAA 1 cut(s) 232
SatI GCNGC 2 cut(s) 130, 205
Sau96I GGNCC 3 cut(s) 9, 71, 93
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 61
SetI ASST 2 cut(s) 98, 131
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 3 cut(s) 9, 71, 93
SmiMI CAYNNNNRTG 1 cut(s) 192
SsiI CCGC 2 cut(s) 7, 205
StyD4I CCNGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 149, 157
TauI GCSGC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 232
Tru9I TTAA 1 cut(s) 232
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 1 cut(s) 98
TspGWI ACGGA 1 cut(s) 167
VpaK11BI GGWCC 3 cut(s) 9, 71, 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.