FvH4_2g25190

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001620
Physical Location & Seq
Reverse (-)
20523814 .. 20524677
864 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25190.t1

Sequence Viewer

Length: 243 bp
ATGGAGGGTTTCGTGCATATGTTTCCGACTTTCTTCATCGCTCTTCTGCTTCTGGTGGCTACTGGGTTGGGACCAAAGGTAATGGTTGCTCATGAGGCCATAACTTGTGAGACTCAGAGTATCAAATATGAGGGAATGTGCTCGAGGGAGAAACCCTGTGCATCACTTTGCCAAACTAAGGGCTACAGTAGAGGCGAATGCCATGGTGTTTACAAGATATGCGTCTGCGCTACACAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0016020 GO:0030312 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

81

Amino Acids

8.77

Weight (kDa)

7.55

Isoelectric Point (pI)

14.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 35 - 80 3e-11 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 178
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 143
BcoDI GTCTC 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 71
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 67
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 143
Bsp19I CCATGG 1 cut(s) 202
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 127
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 72
BspQI GCTCTTC 1 cut(s) 48
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 202
BssT1I CCWWGG 1 cut(s) 202
Bst4CI ACNGT 2 cut(s) 188, 237
Bst6I CTCTTC 1 cut(s) 48
BstDEI CTNAG 2 cut(s) 114, 177
BstDSI CCRYGG 1 cut(s) 202
BstHHI GCGC 1 cut(s) 230
BstMAI GTCTC 1 cut(s) 104
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 184
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 242
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 211
CviAII CATG 2 cut(s) 92, 203
CviJI RGCY 3 cut(s) 59, 98, 183
CviKI_1 RGCY 3 cut(s) 59, 98, 183
DdeI CTNAG 2 cut(s) 114, 177
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 2 cut(s) 95, 206
FaiI YATR 7 cut(s) 18, 20, 93, 101, 129, 204, 220
FaqI GGGAC 1 cut(s) 84
FatI CATG 2 cut(s) 91, 202
FauNDI CATATG 1 cut(s) 18
GlaI GCGC 1 cut(s) 229
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 230
Hin1II CATG 2 cut(s) 95, 206
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HinfI GANTC 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 27, 117
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 188, 237
HpyCH4V TGCA 2 cut(s) 16, 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpyF3I CTNAG 2 cut(s) 114, 177
Hsp92II CATG 2 cut(s) 95, 206
HspAI GCGC 1 cut(s) 228
LguI GCTCTTC 1 cut(s) 48
LpnPI CCDG 3 cut(s) 38, 48, 169
LweI GCATC 1 cut(s) 170
MboII GAAGA 2 cut(s) 25, 35
MhlI GDGCHC 1 cut(s) 143
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 4 cut(s) 88, 124, 138, 185
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 95
NcoI CCATGG 1 cut(s) 202
NdeI CATATG 1 cut(s) 18
NlaIII CATG 2 cut(s) 95, 206
NlaIV GGNNCC 1 cut(s) 72
PaeR7I CTCGAG 1 cut(s) 142
PagI TCATGA 1 cut(s) 91
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 1 cut(s) 203
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 71
PspXI VCTCGAGB 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 48
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 106
SduI GDGCHC 1 cut(s) 143
SetI ASST 1 cut(s) 81
SfaNI GCATC 1 cut(s) 170
SfcI CTRYAG 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 142
SinI GGWCC 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 2 cut(s) 188, 237
TaqI TCGA 1 cut(s) 143
TscAI CASTG 1 cut(s) 242
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 71
XhoI CTCGAG 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.