AT1G65020

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003421
Physical Location & Seq
Reverse (-)
24154344 .. 24156616
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65020.1

Sequence Viewer

Length: 960 bp
ATGTGGATTGAAATCATCTGCGGTCTCGTCATCTACAAACTCGTCCGCCGTTTCTTCTACGACGACGAATTCTCCGACGTCGAAACTTCCGATTCCACTGCTCTCTTCTCCGTTGCTCACAGACTTGAGAAACTTTATGGTGGAAAAGCTTATGTAGGACTTCGAATTCCAGATGCCGACACTGCTTCTAGACAAGACATTGATGTTGTTCTTGTTACTAAAGGAGATGTTGTAGTGATTTCTGTGAAGAATCTATCGGGGATTGTTACGGTAACTAGTGATGGTAGCTGGGTTTGTGAAGGTGGCAAGCATCATACTACTGAGACTTTCCCTGATCCTCTGGCGGAGGTCAAGAAACAAGCTTCAGTTCTTGAATCATATCTTGAACAGAGGGGAGTTACTTTACTAGAAGGAAATGTGTCTTGCAAAGTTGTCATTCCCAATCCAAGTTTTCGTACAATTCATGCATTCCCGAGTGAGGTAATTACCTATGAAGATTGGCAACAGCTAAAACCAGTATCAAAAAGTAAAATTTCTGGTTGGGTTAAAGGCGCATTCAGCACCGGAAAAGAGATGCAGGAATCTTCGCATCAAAAACTTAATTTCATCCTTGGCACAGCACCAACGTGGGATAGAGTGGAGCTTAAAAGTAGCAAAATTGTGTTAGGGGAATTCCTCGAGTTTAAAGGAAAGCAGGAAGATACCCTGGCTTTAAGAAATATCAAAAGGTCAAAAGTCGACCGTGTCTCTATTCAGAAAACAAGCATGTTTGGATTAGCTCCTTCGAGGTTGCAGGTTCTGTATTCGTATAGGGACTACAGAAGTGAAGGCAGTTCAGGGGCAGAGTCGAAAGAAGTGACTGTCAGGTCGAGCACTGAGGTTCTGTTTCAGCCACGAGACTCCACAAAGATGAAGAAATTCAAGCTATCGTCGCTTGTCTCCATTTCACTAAGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

319

Amino Acids

35.62

Weight (kDa)

8.75

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 37 - 146 1.5e-14 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 865
AatII GACGTC 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 784
AccI GTMKAC 1 cut(s) 738
AciI CCGC 3 cut(s) 21, 46, 344
AclWI GGATC 1 cut(s) 329
AcsI RAATTY 5 cut(s) 68, 165, 531, 671, 917
AcuI CTGAAG 1 cut(s) 348
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 953
AfaI GTAC 1 cut(s) 457
AfiI CCNNNNNNNGG 1 cut(s) 478
AgsI TTSAA 4 cut(s) 11, 374, 386, 922
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 1 cut(s) 705
AleI CACNNNNGTG 1 cut(s) 625
AluBI AGCT 7 cut(s) 149, 288, 362, 508, 643, 779, 925
AluI AGCT 7 cut(s) 149, 288, 362, 508, 643, 779, 925
Alw21I GWGCWC 1 cut(s) 875
Alw26I GTCTC 5 cut(s) 29, 317, 751, 891, 943
AlwI GGATC 1 cut(s) 329
AlwNI CAGNNNCTG 1 cut(s) 799
Ama87I CYCGRG 2 cut(s) 472, 677
ApoI RAATTY 5 cut(s) 68, 165, 531, 671, 917
Asp700I GAANNNNTTC 1 cut(s) 917
AspLEI GCGC 1 cut(s) 554
AsuII TTCGAA 1 cut(s) 163
AvaI CYCGRG 2 cut(s) 472, 677
BauI CACGAG 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 875
BccI CCATC 1 cut(s) 275
BceAI ACGGC 1 cut(s) 33
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 1 cut(s) 707
BcoDI GTCTC 5 cut(s) 29, 317, 751, 891, 943
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 3 cut(s) 189, 276, 407
BfmI CTRYAG 1 cut(s) 817
BfuAI ACCTGC 1 cut(s) 784
Bme1390I CCNGG 1 cut(s) 707
BmeT110I CYCGRG 2 cut(s) 472, 677
BmrFI CCNGG 1 cut(s) 707
BmsI GCATC 4 cut(s) 163, 319, 564, 598
Bpu14I TTCGAA 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 610, 705
BsaWI WCCGGW 1 cut(s) 563
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 515
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 2 cut(s) 610, 705
BseGI GGATG 1 cut(s) 606
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMII CTCAG 2 cut(s) 312, 867
BseNI ACTGG 1 cut(s) 515
BseYI CCCAGC 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 742
BsiEI CGRYCG 1 cut(s) 742
BsiHKAI GWGCWC 1 cut(s) 875
BsiHKCI CYCGRG 2 cut(s) 472, 677
BsiSI CCGG 1 cut(s) 564
BslFI GGGAC 1 cut(s) 827
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 5 cut(s) 29, 317, 751, 891, 943
BsmFI GGGAC 1 cut(s) 827
BsmI GAATGC 2 cut(s) 467, 554
Bso31I GGTCTC 1 cut(s) 29
BsoBI CYCGRG 2 cut(s) 472, 677
Bsp119I TTCGAA 1 cut(s) 163
Bsp1286I GDGCHC 1 cut(s) 875
Bsp143I GATC 1 cut(s) 334
BspACI CCGC 3 cut(s) 21, 46, 344
BspCNI CTCAG 2 cut(s) 313, 868
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 1 cut(s) 329
BspT104I TTCGAA 1 cut(s) 163
BspTNI GGTCTC 1 cut(s) 29
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 610, 705
BssMI GATC 1 cut(s) 334
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 894
BssT1I CCWWGG 1 cut(s) 610
Bst2BI CACGAG 1 cut(s) 894
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 3 cut(s) 271, 743, 862
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 78
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 3 cut(s) 321, 876, 950
BstF5I GGATG 1 cut(s) 606
BstHHI GCGC 1 cut(s) 554
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 5 cut(s) 29, 317, 751, 891, 943
BstMBI GATC 1 cut(s) 334
BstMCI CGRYCG 1 cut(s) 742
BstMWI GCNNNNNNNGC 3 cut(s) 182, 558, 931
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 1 cut(s) 705
BstSFI CTRYAG 1 cut(s) 817
BtsCI GGATG 1 cut(s) 606
BtsI GCAGTG 2 cut(s) 96, 180
BtsIMutI CAGTG 3 cut(s) 96, 180, 873
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 1 cut(s) 308
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 1 cut(s) 554
Csp6I GTAC 1 cut(s) 456
CviAII CATG 2 cut(s) 464, 766
CviJI RGCY 9 cut(s) 149, 288, 362, 508, 643, 710, 779, 892, 925
CviKI_1 RGCY 9 cut(s) 149, 288, 362, 508, 643, 710, 779, 892, 925
CviQI GTAC 1 cut(s) 456
DdeI CTNAG 3 cut(s) 321, 876, 950
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
DraI TTTAAA 1 cut(s) 685
DraIII CACNNNGTG 1 cut(s) 953
DrdI GACNNNNNNGTC 1 cut(s) 865
DseDI GACNNNNNNGTC 1 cut(s) 865
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 2 cut(s) 35, 359
Eco130I CCWWGG 1 cut(s) 610
Eco31I GGTCTC 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 348
Eco88I CYCGRG 2 cut(s) 472, 677
EcoRI GAATTC 3 cut(s) 68, 165, 671
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 610
FaeI CATG 2 cut(s) 467, 769
FaiI YATR 8 cut(s) 138, 153, 315, 379, 465, 492, 767, 810
FaqI GGGAC 1 cut(s) 827
FatI CATG 2 cut(s) 463, 765
FblI GTMKAC 1 cut(s) 738
FokI GGATG 1 cut(s) 593
FspBI CTAG 3 cut(s) 189, 276, 407
GlaI GCGC 1 cut(s) 553
GsaI CCCAGC 1 cut(s) 292
HapII CCGG 1 cut(s) 564
HhaI GCGC 1 cut(s) 554
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 2 cut(s) 467, 769
Hin6I GCGC 1 cut(s) 552
HinP1I GCGC 1 cut(s) 552
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 147, 360
HinfI GANTC 6 cut(s) 92, 250, 374, 581, 845, 899
HpaII CCGG 1 cut(s) 564
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 3 cut(s) 76, 91, 756
Hpy188III TCNNGA 6 cut(s) 170, 189, 352, 371, 383, 472
Hpy8I GTNNAC 1 cut(s) 739
Hpy99I CGWCG 5 cut(s) 65, 68, 80, 83, 934
HpyAV CCTTC 4 cut(s) 293, 404, 792, 821
HpyCH4III ACNGT 3 cut(s) 271, 743, 862
HpyCH4IV ACGT 2 cut(s) 78, 626
HpyCH4V TGCA 4 cut(s) 426, 467, 577, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 558, 931
HpyF3I CTNAG 3 cut(s) 321, 876, 950
HpySE526I ACGT 2 cut(s) 78, 626
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 2 cut(s) 467, 769
HspAI GCGC 1 cut(s) 552
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 2 cut(s) 640, 784
LweI GCATC 4 cut(s) 163, 319, 564, 598
MaeI CTAG 3 cut(s) 189, 276, 407
MaeII ACGT 2 cut(s) 78, 626
MaeIII GTNAC 5 cut(s) 214, 265, 271, 397, 856
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 7 cut(s) 46, 97, 259, 506, 576, 710, 925
MhlI GDGCHC 1 cut(s) 875
MluCI AATT 9 cut(s) 68, 165, 459, 483, 531, 601, 657, 671, 917
MlyI GAGTC 2 cut(s) 854, 893
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 7 cut(s) 340, 348, 384, 472, 686, 780, 871
Mph1103I ATGCAT 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 5 cut(s) 546, 600, 645, 684, 713
MslI CAYNNNNRTG 2 cut(s) 625, 908
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 1 cut(s) 707
Mva1269I GAATGC 2 cut(s) 467, 554
MvaI CCWGG 1 cut(s) 707
MwoI GCNNNNNNNGC 3 cut(s) 182, 558, 931
NdeII GATC 1 cut(s) 334
NlaIII CATG 2 cut(s) 467, 769
NmuCI GTSAC 1 cut(s) 856
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 1 cut(s) 769
NspV TTCGAA 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 677
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 87
PctI GAATGC 2 cut(s) 467, 554
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 4 cut(s) 92, 250, 374, 581
PflFI GACNNNGTC 1 cut(s) 743
PleI GAGTC 2 cut(s) 853, 893
PpsI GAGTC 2 cut(s) 853, 893
Psp6I CCWGG 1 cut(s) 705
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 1 cut(s) 705
PspXI VCTCGAGB 1 cut(s) 677
PstNI CAGNNNCTG 1 cut(s) 799
PsyI GACNNNGTC 1 cut(s) 743
RsaI GTAC 1 cut(s) 457
RsaNI GTAC 1 cut(s) 456
RseI CAYNNNNRTG 2 cut(s) 625, 908
SalI GTCGAC 1 cut(s) 737
SaqAI TTAA 5 cut(s) 546, 600, 645, 684, 713
Sau3AI GATC 1 cut(s) 334
SchI GAGTC 2 cut(s) 854, 893
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 1 cut(s) 875
SfaNI GCATC 4 cut(s) 163, 319, 564, 598
SfcI CTRYAG 1 cut(s) 817
Sfr274I CTCGAG 1 cut(s) 677
SfuI TTCGAA 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 677
SmiMI CAYNNNNRTG 2 cut(s) 625, 908
SmlI CTYRAG 2 cut(s) 125, 677
SmoI CTYRAG 2 cut(s) 125, 677
SpeI ACTAGT 1 cut(s) 275
Sse9I AATT 9 cut(s) 68, 165, 459, 483, 531, 601, 657, 671, 917
SsiI CCGC 3 cut(s) 21, 46, 344
SspMI CTAG 3 cut(s) 189, 276, 407
StyD4I CCNGG 1 cut(s) 705
StyI CCWWGG 1 cut(s) 610
TaaI ACNGT 3 cut(s) 271, 743, 862
TaiI ACGT 2 cut(s) 81, 629
TaqI TCGA 7 cut(s) 81, 163, 678, 738, 785, 848, 869
TasI AATT 9 cut(s) 68, 165, 459, 483, 531, 601, 657, 671, 917
TfiI GAWTC 4 cut(s) 92, 250, 374, 581
Tru1I TTAA 5 cut(s) 546, 600, 645, 684, 713
Tru9I TTAA 5 cut(s) 546, 600, 645, 684, 713
TscAI CASTG 3 cut(s) 103, 187, 880
TseFI GTSAC 1 cut(s) 856
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 4 cut(s) 452, 507, 595, 926
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 3 cut(s) 103, 187, 880
Tth111I GACNNNGTC 1 cut(s) 743
XapI RAATTY 5 cut(s) 68, 165, 531, 671, 917
XbaI TCTAGA 1 cut(s) 188
XceI RCATGY 1 cut(s) 769
XhoI CTCGAG 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 738
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 3 cut(s) 189, 276, 407
ZraI GACGTC 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.