Rh1AG189400

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003421
Physical Location & Seq
Forward (+)
36073677 .. 36088621
14945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG189400.1

Sequence Viewer

Length: 417 bp
ATGGGTATACGGGTATTGAGCTCCTTAGAACTAAAGCTGCTAGTTCGAAGACTGTACAAGTTGATCTTCTCCGACGGCGATGACGTCTTGGGTGTCGAGACCTCTGATTCTACTACTCTCTTCGCCATCGCTGACAGGCTTGCTAAGCTCTATGATGGCGACAAGGTCTCAGTTGGCCTCCGGATCCCCAACGCCGATACCGCTTCCCACCAAGACGAAGACGAAGATGAAGCTCAGATTCAGACCAAATCTGAGATGATTGCAATCCTGGGGAAGAGTTTCGATGGAGTCAGAGAGTCTTTCTTCGCTTCCGAAGTGGAACTTCACCAAGATTTTGAGGAACTTCACAGGAACGCTCATCTGGAGATGATTAAACTCTCGAAGAAGTTTGCAGCATTGAAGAGGAAGTATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

138

Amino Acids

15.62

Weight (kDa)

5.22

Isoelectric Point (pI)

26.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
AccI GTMKAC 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 1 cut(s) 201
AclWI GGATC 2 cut(s) 178, 191
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 56
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 4 cut(s) 21, 37, 148, 233
AluI AGCT 4 cut(s) 21, 37, 148, 233
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 2 cut(s) 92, 172
AlwI GGATC 2 cut(s) 178, 191
Aor13HI TCCGGA 1 cut(s) 180
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 2 cut(s) 37, 392
Asp700I GAANNNNTTC 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 317
AsuII TTCGAA 1 cut(s) 46
BamHI GGATCC 1 cut(s) 183
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 2 cut(s) 55, 225
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 24, 404
BccI CCATC 3 cut(s) 134, 149, 278
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 92, 172
BfaI CTAG 1 cut(s) 41
BisI GCNGC 2 cut(s) 38, 393
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 2 cut(s) 39, 394
Bme1390I CCNGG 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 269
BpiI GAAGAC 2 cut(s) 55, 225
BpmI CTGGAG 1 cut(s) 383
Bpu1102I GCTNAGC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 2 cut(s) 92, 172
BsaJI CCNNGG 1 cut(s) 268
BsaWI WCCGGW 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 263
BseAI TCCGGA 1 cut(s) 180
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 263
BseMII CTCAG 3 cut(s) 183, 243, 248
BseXI GCAGC 2 cut(s) 24, 404
BshFI GGCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 181
BsmAI GTCTC 2 cut(s) 92, 172
BsnI GGCC 1 cut(s) 177
Bso31I GGTCTC 2 cut(s) 92, 172
Bsp119I TTCGAA 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 23
Bsp13I TCCGGA 1 cut(s) 180
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 2 cut(s) 63, 183
Bsp1720I GCTNAGC 1 cut(s) 144
BspACI CCGC 1 cut(s) 201
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 3 cut(s) 182, 244, 247
BspEI TCCGGA 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 185
BspPI GGATC 2 cut(s) 178, 191
BspT104I TTCGAA 1 cut(s) 46
BspTNI GGTCTC 2 cut(s) 92, 172
BsrGI TGTACA 1 cut(s) 54
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 2 cut(s) 63, 183
BssNAI GTATAC 1 cut(s) 8
BssNI GRCGYC 1 cut(s) 84
Bst1107I GTATAC 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 54
Bst6I CTCTTC 3 cut(s) 125, 269, 395
BstACI GRCGYC 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 5 cut(s) 25, 144, 169, 234, 252
BstKTI GATC 2 cut(s) 66, 186
BstMAI GTCTC 2 cut(s) 92, 172
BstMBI GATC 2 cut(s) 63, 183
BstMWI GCNNNNNNNGC 2 cut(s) 145, 200
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 267
BstV1I GCAGC 2 cut(s) 24, 404
BstV2I GAAGAC 2 cut(s) 55, 225
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BstZ17I GTATAC 1 cut(s) 8
BsuRI GGCC 1 cut(s) 177
BtgZI GCGATG 2 cut(s) 93, 112
Cac8I GCNNGC 1 cut(s) 141
Csp6I GTAC 1 cut(s) 55
CviJI RGCY 6 cut(s) 21, 37, 139, 148, 177, 233
CviKI_1 RGCY 6 cut(s) 21, 37, 139, 148, 177, 233
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 5 cut(s) 25, 144, 169, 234, 252
DpnI GATC 2 cut(s) 65, 185
DpnII GATC 2 cut(s) 63, 183
Eam1104I CTCTTC 3 cut(s) 125, 269, 395
EarI CTCTTC 3 cut(s) 125, 269, 395
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 2 cut(s) 92, 172
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 23
FaiI YATR 2 cut(s) 8, 153
FalI AAGNNNNNCTT 4 cut(s) 50, 82, 306, 338
FblI GTMKAC 1 cut(s) 7
Fnu4HI GCNGC 2 cut(s) 38, 393
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 38, 393
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 38, 393
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 1 cut(s) 177
HapII CCGG 1 cut(s) 181
Hin1I GRCGYC 1 cut(s) 84
HinfI GANTC 4 cut(s) 107, 238, 288, 296
HpaII CCGG 1 cut(s) 181
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 1 cut(s) 8
Hpy188I TCNGA 7 cut(s) 73, 106, 237, 243, 253, 293, 313
Hpy188III TCNNGA 4 cut(s) 97, 181, 362, 379
Hpy8I GTNNAC 1 cut(s) 8
Hpy99I CGWCG 1 cut(s) 77
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 263, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 200
HpyF3I CTNAG 5 cut(s) 25, 144, 169, 234, 252
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 84
Kpn2I TCCGGA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 63, 183
LmnI GCTCC 1 cut(s) 26
LpnPI CCDG 6 cut(s) 121, 194, 254, 281, 334, 347
Lsp1109I GCAGC 2 cut(s) 24, 404
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 84
MalI GATC 2 cut(s) 65, 185
MboI GATC 2 cut(s) 63, 183
MboII GAAGA 9 cut(s) 58, 60, 112, 230, 236, 286, 295, 394, 412
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 23
MlyI GAGTC 2 cut(s) 297, 305
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 4 cut(s) 112, 188, 331, 396
MroI TCCGGA 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 1 cut(s) 372
MspI CCGG 1 cut(s) 181
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 145, 200
NdeII GATC 2 cut(s) 63, 183
NlaIV GGNNCC 1 cut(s) 185
NspV TTCGAA 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 107, 238
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 2 cut(s) 39, 394
PleI GAGTC 2 cut(s) 296, 304
PpsI GAGTC 2 cut(s) 296, 304
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 185
PsuI RGATCY 1 cut(s) 183
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 38, 393
Sau3AI GATC 2 cut(s) 63, 183
SchI GAGTC 2 cut(s) 297, 305
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 1 cut(s) 23
SetI ASST 7 cut(s) 23, 39, 87, 104, 150, 168, 235
SfuI TTCGAA 1 cut(s) 46
SsiI CCGC 1 cut(s) 201
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 54
TaiI ACGT 1 cut(s) 87
TaqI TCGA 4 cut(s) 46, 96, 282, 380
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 2 cut(s) 107, 238
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseI GCWGC 2 cut(s) 37, 392
TspDTI ATGAA 1 cut(s) 243
Tth111I GACNNNGTC 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 7
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 1 cut(s) 41
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.