Rorug01G0448200

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003421
Physical Location & Seq
Forward (+)
54432183 .. 54433617
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0448200.1

Sequence Viewer

Length: 525 bp
ATGGAATCTCCGATAGCTTCCTGCAGCCAACATCTTCAGACCCAAGAATCTGGGAAAATTTCACTGCTGAATGACGATTTACTTGGCCTAATCTTAACAAGGATTAAAGTTAAAGGTGACAGAAAATCGTGCTCAGAGGTTTGCAAGCAATGGTTGAGAGTTGAAGGACTGAGGCGTTCATCACTTGTGGTTCTTCAACGACACGATTTCCGTATTAGTGCACTCACTAGGTTCCCGAATTTGACTAAATTCGAAACATGGAAGCCTATGATCGACACCGACCTTGAGTTCTTGGCCAAAACATGTCCGCAACTTCAGATCACCAGGTTCAATTGGCGTCAGGAAGATGGTGATGGTGATGGTGGTGTTGTGAGTGAAAAAGGTCTAAGGGCTTTGGGAAATGGGTGTCCTAAACTGGCTTGGGTTTTAGTTTCCGGGGAAGGAAGGGTAGGGGATTCTGGAGTTGGTGTGCTTGTACATTTTGCTGCTAACCCTGAAAAAATTGGTCATTGTGAGGTGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

174

Amino Acids

19.34

Weight (kDa)

8.57

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 24 - 60 3e-06 F-box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 3 cut(s) 57, 238, 248
AcuI CTGAAG 2 cut(s) 20, 299
AcyI GRCGYC 1 cut(s) 337
AfaI GTAC 1 cut(s) 477
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 3 cut(s) 164, 197, 331
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw21I GWGCWC 2 cut(s) 134, 223
Alw44I GTGCAC 1 cut(s) 219
AoxI GGCC 2 cut(s) 85, 294
ApaLI GTGCAC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 24, 485
ApoI RAATTY 3 cut(s) 57, 238, 248
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 4 cut(s) 128, 313, 362, 368
AsuII TTCGAA 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 223
BalI TGGCCA 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 134, 223
BbvI GCAGC 2 cut(s) 36, 472
BccI CCATC 3 cut(s) 341, 347, 353
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 1 cut(s) 436
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 2 cut(s) 25, 486
BlsI GCNGC 2 cut(s) 26, 487
Bme1390I CCNGG 2 cut(s) 325, 436
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 2 cut(s) 325, 436
BpmI CTGGAG 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 436
BsaHI GRCGYC 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bse1I ACTGG 1 cut(s) 420
Bse3DI GCAATG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 155
BseMII CTCAG 2 cut(s) 147, 161
BseNI ACTGG 1 cut(s) 420
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 2 cut(s) 36, 472
BshFI GGCC 2 cut(s) 87, 296
BsiHKAI GWGCWC 2 cut(s) 134, 223
BsiSI CCGG 1 cut(s) 435
BsnI GGCC 2 cut(s) 87, 296
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 2 cut(s) 134, 223
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 2 cut(s) 270, 318
BspACI CCGC 1 cut(s) 308
BspANI GGCC 2 cut(s) 87, 296
BspCNI CTCAG 2 cut(s) 146, 162
BspLI GGNNCC 1 cut(s) 233
BspMAI CTGCAG 1 cut(s) 26
BspT104I TTCGAA 1 cut(s) 252
BsrDI GCAATG 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 1 cut(s) 420
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 2 cut(s) 270, 318
BssNI GRCGYC 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 325
BstACI GRCGYC 1 cut(s) 337
BstAUI TGTACA 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 3 cut(s) 133, 170, 386
BstKTI GATC 2 cut(s) 273, 321
BstMBI GATC 2 cut(s) 270, 318
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 323, 434
BstSFI CTRYAG 1 cut(s) 22
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 36, 472
BstXI CCANNNNNNTGG 1 cut(s) 50
BsuRI GGCC 2 cut(s) 87, 296
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 62
Cac8I GCNNGC 1 cut(s) 146
CseI GACGC 1 cut(s) 326
CsiI ACCWGGT 1 cut(s) 323
Csp6I GTAC 1 cut(s) 476
CviAII CATG 2 cut(s) 258, 303
CviJI RGCY 7 cut(s) 17, 27, 87, 265, 296, 392, 419
CviKI_1 RGCY 7 cut(s) 17, 27, 87, 265, 296, 392, 419
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 3 cut(s) 133, 170, 386
DpnI GATC 2 cut(s) 272, 320
DpnII GATC 2 cut(s) 270, 318
EaeI YGGCCR 1 cut(s) 294
Eco57I CTGAAG 2 cut(s) 20, 299
EcoRII CCWGG 1 cut(s) 323
FaeI CATG 2 cut(s) 261, 306
FaiI YATR 3 cut(s) 259, 269, 304
FatI CATG 2 cut(s) 257, 302
Fnu4HI GCNGC 2 cut(s) 25, 486
Fsp4HI GCNGC 2 cut(s) 25, 486
FspBI CTAG 1 cut(s) 228
GluI GCNGC 2 cut(s) 25, 486
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 2 cut(s) 87, 296
HapII CCGG 1 cut(s) 435
HgaI GACGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 337
Hin1II CATG 2 cut(s) 261, 306
HinfI GANTC 3 cut(s) 5, 47, 455
HpaII CCGG 1 cut(s) 435
HphI GGTGA 4 cut(s) 128, 313, 362, 368
Hpy166II GTNNAC 1 cut(s) 221
Hpy188I TCNGA 4 cut(s) 12, 39, 136, 318
Hpy188III TCNNGA 3 cut(s) 235, 341, 459
Hpy8I GTNNAC 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 158, 434, 438
HpyCH4V TGCA 3 cut(s) 24, 144, 221
HpyF3I CTNAG 3 cut(s) 133, 170, 386
Hsp92I GRCGYC 1 cut(s) 337
Hsp92II CATG 2 cut(s) 261, 306
Kzo9I GATC 2 cut(s) 270, 318
LpnPI CCDG 9 cut(s) 34, 36, 310, 326, 337, 401, 444, 448, 507
Lsp1109I GCAGC 2 cut(s) 36, 472
MabI ACCWGGT 1 cut(s) 323
MaeI CTAG 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 116
MalI GATC 2 cut(s) 272, 320
MboI GATC 2 cut(s) 270, 318
MboII GAAGA 3 cut(s) 26, 185, 356
MfeI CAATTG 1 cut(s) 331
MhlI GDGCHC 2 cut(s) 134, 223
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 5 cut(s) 57, 238, 248, 331, 501
MluNI TGGCCA 1 cut(s) 296
MnlI CCTC 3 cut(s) 130, 165, 508
Mox20I TGGCCA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 296
MseI TTAA 3 cut(s) 95, 105, 111
Msp20I TGGCCA 1 cut(s) 296
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 2 cut(s) 325, 436
MunI CAATTG 1 cut(s) 331
MvaI CCWGG 1 cut(s) 325
NciI CCSGG 1 cut(s) 436
NdeII GATC 2 cut(s) 270, 318
NlaIII CATG 2 cut(s) 261, 306
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 306
NspV TTCGAA 1 cut(s) 252
PciI ACATGT 1 cut(s) 302
PfeI GAWTC 3 cut(s) 5, 47, 455
PkrI GCNGC 2 cut(s) 26, 487
PscI ACATGT 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 233
PstI CTGCAG 1 cut(s) 26
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
SaqAI TTAA 3 cut(s) 95, 105, 111
SatI GCNGC 2 cut(s) 25, 486
Sau3AI GATC 2 cut(s) 270, 318
ScrFI CCNGG 2 cut(s) 325, 436
SduI GDGCHC 2 cut(s) 134, 223
SetI ASST 8 cut(s) 19, 118, 141, 233, 285, 329, 385, 519
SexAI ACCWGGT 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 22
SfuI TTCGAA 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 5 cut(s) 57, 238, 248, 331, 501
SsiI CCGC 1 cut(s) 308
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 323, 434
TaqI TCGA 2 cut(s) 252, 273
TasI AATT 5 cut(s) 57, 238, 248, 331, 501
TatI WGTACW 1 cut(s) 475
TfiI GAWTC 3 cut(s) 5, 47, 455
Tru1I TTAA 3 cut(s) 95, 105, 111
Tru9I TTAA 3 cut(s) 95, 105, 111
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 24, 485
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 1 cut(s) 168
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 1 cut(s) 69
VneI GTGCAC 1 cut(s) 219
XapI RAATTY 3 cut(s) 57, 238, 248
XceI RCATGY 1 cut(s) 306
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.