Rw1G039920

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003421
Physical Location & Seq
Forward (+)
67426424 .. 67428562
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G039920.1

Sequence Viewer

Length: 954 bp
ATGTGGTTCGAGATCCTCTGCGGCCTGGTGATTTACAGACTGTACAAGTTCCTCTTCTCCGACGGCGACGACGTCTTGGATGTCGAGACCTCTGATTCTACCGCTCTCTTCGCCCTCTACGATGGCGCCAAGGTCTACGTTGGCCTCCGGATCCCCGACGCCGATACCGCTTCCCCTCAGCACATCGATTTGGTTCTCCTCAACAAAGGGGAGGCCGTGGTGATAGCCGTGAAGAACTTGTCAGGATTCATCTCCGTCAAGCCCGATGGCAGCTGGGTTTCTGAGAGCCTCACTAAACACAAAATAGAGCAGCATCCTGATCCTCTGGCGGAGACAAAAAGACAAGCTTCAATTCTTGAGTCCTATCTTGAACAAAGAGGAGCTGCTTTACCAGAAGGATATTTGTCTTGCAAAGTAATACTTTCCAATCCAAAAGTATGTACCATTCAGTCAAGCAGTTTCCCACCAGAAATCATTACTTATGACCAGTGGGTACAAATGAAACCAGAACCAAAAAGTATGTTTTCTGGTTGGATGAAGGGCGCATTCCGTGGTGGAAAGAAAGAGATGCAAGAATCTATTCATCAGAAGCTTAATTTCATTCTTAGTACAGCACCAATGTGGGATAGATTGGAGCTTAAAGGTAACAAGTATGTTCTTGGGGAATTTCTGGAATTCAAGGGTAAGCAGGAAGATGTTCAGGCTTTGAGGCATATTAAAAGATCAAAAATTAGCCGCCTTGTTGTCCAAAAGACAAGCATGCTTGGATTTGCCCCTTCAAGGCTCCAAGTTCTGTATACTGGTCGTGATTACCGAAGTGAAGGAGCTTCAGCTTCTGAGTGGACAGAAGTAAATGTAAGGTCAAGCACGGAGGTTGTCTTTCAGCTTGACAATTCTTCAAGAGTCCGCAAGTTTAAGCTCTCCTCAATTTGTTCTATGTCTCTGAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

317

Amino Acids

35.68

Weight (kDa)

8.5

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 41 - 136 2.5e-11 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 104
AccI GTMKAC 2 cut(s) 135, 797
AccIII TCCGGA 1 cut(s) 147
AciI CCGC 6 cut(s) 21, 102, 168, 329, 736, 907
AclWI GGATC 4 cut(s) 7, 145, 158, 314
AcsI RAATTY 2 cut(s) 665, 674
AcuI CTGAAG 1 cut(s) 813
AcyI GRCGYC 3 cut(s) 72, 126, 159
AfaI GTAC 4 cut(s) 44, 442, 495, 610
AfiI CCNNNNNNNGG 1 cut(s) 780
AgsI TTSAA 5 cut(s) 351, 371, 679, 780, 900
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 1 cut(s) 619
AluBI AGCT 9 cut(s) 273, 347, 383, 592, 637, 827, 833, 886, 919
AluI AGCT 9 cut(s) 273, 347, 383, 592, 637, 827, 833, 886, 919
Alw26I GTCTC 3 cut(s) 80, 326, 945
AlwI GGATC 4 cut(s) 7, 145, 158, 314
AlwNI CAGNNNCTG 1 cut(s) 836
Aor13HI TCCGGA 1 cut(s) 147
AoxI GGCC 3 cut(s) 22, 142, 213
ApeKI GCWGC 3 cut(s) 270, 310, 383
ApoI RAATTY 2 cut(s) 665, 674
Asp700I GAANNNNTTC 2 cut(s) 579, 696
AspLEI GCGC 2 cut(s) 128, 545
AsuHPI GGTGA 2 cut(s) 40, 232
BamHI GGATCC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 125
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 3 cut(s) 282, 322, 370
BccI CCATC 2 cut(s) 116, 260
BceAI ACGGC 3 cut(s) 79, 200, 212
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 3 cut(s) 80, 326, 945
BfoI RGCGCY 1 cut(s) 129
BisI GCNGC 5 cut(s) 22, 271, 311, 384, 736
BlsI GCNGC 5 cut(s) 23, 272, 312, 385, 737
Bme1390I CCNGG 1 cut(s) 26
BmiI GGNNCC 3 cut(s) 127, 152, 785
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 322, 558
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 377
Bsa29I ATCGAT 1 cut(s) 186
BsaHI GRCGYC 3 cut(s) 72, 126, 159
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 129, 216, 550
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 1 cut(s) 780
Bse1I ACTGG 2 cut(s) 487, 805
BseAI TCCGGA 1 cut(s) 147
BseBI CCWGG 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 186
BseDI CCNNGG 3 cut(s) 129, 216, 550
BseGI GGATG 3 cut(s) 85, 313, 540
BseLI CCNNNNNNNGG 1 cut(s) 780
BseMII CTCAG 4 cut(s) 191, 273, 828, 935
BseNI ACTGG 2 cut(s) 487, 805
BseRI GAGGAG 3 cut(s) 188, 393, 913
BseXI GCAGC 3 cut(s) 282, 322, 370
BseYI CCCAGC 1 cut(s) 273
BshFI GGCC 3 cut(s) 24, 144, 215
BshNI GGYRCC 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 186
BsiSI CCGG 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 780
BsmAI GTCTC 3 cut(s) 80, 326, 945
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 3 cut(s) 24, 144, 215
Bso31I GGTCTC 1 cut(s) 80
Bsp13I TCCGGA 1 cut(s) 147
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 4 cut(s) 12, 150, 319, 722
BspACI CCGC 6 cut(s) 21, 102, 168, 329, 736, 907
BspANI GGCC 3 cut(s) 24, 144, 215
BspCNI CTCAG 4 cut(s) 190, 274, 829, 936
BspDI ATCGAT 1 cut(s) 186
BspEI TCCGGA 1 cut(s) 147
BspLI GGNNCC 3 cut(s) 127, 152, 785
BspPI GGATC 4 cut(s) 7, 145, 158, 314
BspT107I GGYRCC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 2 cut(s) 487, 805
BssECI CCNNGG 3 cut(s) 129, 216, 550
BssMI GATC 4 cut(s) 12, 150, 319, 722
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 3 cut(s) 72, 126, 159
BssT1I CCWWGG 1 cut(s) 129
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 2 cut(s) 59, 113
BstACI GRCGYC 3 cut(s) 72, 126, 159
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 761
BstDEI CTNAG 5 cut(s) 177, 282, 605, 837, 944
BstDSI CCRYGG 2 cut(s) 216, 550
BstF5I GGATG 3 cut(s) 85, 313, 540
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 2 cut(s) 128, 545
BstKTI GATC 4 cut(s) 15, 153, 322, 725
BstMAI GTCTC 3 cut(s) 80, 326, 945
BstMBI GATC 4 cut(s) 12, 150, 319, 722
BstMWI GCNNNNNNNGC 2 cut(s) 110, 167
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 282, 322, 370
BstX2I RGATCY 2 cut(s) 12, 150
BstYI RGATCY 2 cut(s) 12, 150
BstZ17I GTATAC 1 cut(s) 798
Bsu15I ATCGAT 1 cut(s) 186
BsuRI GGCC 3 cut(s) 24, 144, 215
BsuTUI ATCGAT 1 cut(s) 186
BtgI CCRYGG 2 cut(s) 216, 550
BtsCI GGATG 3 cut(s) 85, 313, 540
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 1 cut(s) 761
CaiI CAGNNNCTG 1 cut(s) 836
CfoI GCGC 2 cut(s) 128, 545
ClaI ATCGAT 1 cut(s) 186
CseI GACGC 1 cut(s) 167
Csp6I GTAC 4 cut(s) 43, 441, 494, 609
CviAII CATG 1 cut(s) 760
CviQI GTAC 4 cut(s) 43, 441, 494, 609
DdeI CTNAG 5 cut(s) 177, 282, 605, 837, 944
DinI GGCGCC 1 cut(s) 127
DpnI GATC 4 cut(s) 14, 152, 321, 724
DpnII GATC 4 cut(s) 12, 150, 319, 722
Eam1104I CTCTTC 2 cut(s) 59, 113
EarI CTCTTC 2 cut(s) 59, 113
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 813
EcoRI GAATTC 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 129
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 1 cut(s) 763
FaiI YATR 8 cut(s) 439, 483, 521, 654, 714, 761, 798, 938
FalI AAGNNNNNCTT 6 cut(s) 38, 70, 331, 363, 405, 437
FatI CATG 1 cut(s) 759
FblI GTMKAC 2 cut(s) 135, 797
Fnu4HI GCNGC 5 cut(s) 22, 271, 311, 384, 736
FokI GGATG 3 cut(s) 92, 300, 547
Fsp4HI GCNGC 5 cut(s) 22, 271, 311, 384, 736
GlaI GCGC 2 cut(s) 127, 544
GluI GCNGC 5 cut(s) 22, 271, 311, 384, 736
GsaI CCCAGC 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 3 cut(s) 24, 144, 215
HapII CCGG 1 cut(s) 148
HgaI GACGC 1 cut(s) 167
HhaI GCGC 2 cut(s) 128, 545
Hin1I GRCGYC 3 cut(s) 72, 126, 159
Hin1II CATG 1 cut(s) 763
Hin6I GCGC 2 cut(s) 126, 543
HinP1I GCGC 2 cut(s) 126, 543
HindIII AAGCTT 2 cut(s) 345, 590
HinfI GANTC 5 cut(s) 95, 246, 359, 575, 903
HpaII CCGG 1 cut(s) 148
HphI GGTGA 2 cut(s) 40, 232
Hpy166II GTNNAC 3 cut(s) 136, 798, 843
Hpy188I TCNGA 6 cut(s) 61, 94, 283, 588, 838, 945
Hpy8I GTNNAC 3 cut(s) 136, 798, 843
Hpy99I CGWCG 4 cut(s) 65, 71, 74, 161
HpyAV CCTTC 4 cut(s) 389, 532, 786, 815
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 2 cut(s) 72, 138
HpyCH4V TGCA 2 cut(s) 411, 571
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 167
HpyF3I CTNAG 5 cut(s) 177, 282, 605, 837, 944
HpySE526I ACGT 2 cut(s) 72, 138
Hsp92I GRCGYC 3 cut(s) 72, 126, 159
Hsp92II CATG 1 cut(s) 763
HspAI GCGC 2 cut(s) 126, 543
KasI GGCGCC 1 cut(s) 125
Kpn2I TCCGGA 1 cut(s) 147
Kzo9I GATC 4 cut(s) 12, 150, 319, 722
LmnI GCTCC 4 cut(s) 380, 634, 789, 824
Lsp1109I GCAGC 3 cut(s) 282, 322, 370
LweI GCATC 2 cut(s) 322, 558
MaeII ACGT 2 cut(s) 72, 138
MaeIII GTNAC 1 cut(s) 644
MalI GATC 4 cut(s) 14, 152, 321, 724
MbiI CCGCTC 1 cut(s) 104
MboI GATC 4 cut(s) 12, 150, 319, 722
MboII GAAGA 5 cut(s) 46, 100, 244, 704, 888
MflI RGATCY 2 cut(s) 12, 150
MluCI AATT 7 cut(s) 351, 595, 665, 674, 729, 892, 927
Mly113I GGCGCC 1 cut(s) 126
MlyI GAGTC 2 cut(s) 368, 912
MmeI TCCRAC 2 cut(s) 84, 512
MroI TCCGGA 1 cut(s) 147
MroXI GAANNNNTTC 2 cut(s) 579, 696
MseI TTAA 5 cut(s) 594, 639, 717, 915, 952
MslI CAYNNNNRTG 1 cut(s) 619
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 110, 167
NarI GGCGCC 1 cut(s) 126
NdeII GATC 4 cut(s) 12, 150, 319, 722
NlaIII CATG 1 cut(s) 763
NlaIV GGNNCC 3 cut(s) 127, 152, 785
NspI RCATGY 1 cut(s) 763
OliI CACNNNNGTG 1 cut(s) 619
PaeI GCATGC 1 cut(s) 763
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 117, 811
PctI GAATGC 1 cut(s) 545
PdmI GAANNNNTTC 2 cut(s) 579, 696
PfeI GAWTC 3 cut(s) 95, 246, 575
PflFI GACNNNGTC 1 cut(s) 71
PkrI GCNGC 5 cut(s) 23, 272, 312, 385, 737
PleI GAGTC 2 cut(s) 367, 911
PluTI GGCGCC 1 cut(s) 129
PpsI GAGTC 2 cut(s) 367, 911
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 127, 152, 785
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 2 cut(s) 12, 150
PsyI GACNNNGTC 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 273
RsaI GTAC 4 cut(s) 44, 442, 495, 610
RsaNI GTAC 4 cut(s) 43, 441, 494, 609
RseI CAYNNNNRTG 1 cut(s) 619
SaqAI TTAA 5 cut(s) 594, 639, 717, 915, 952
SatI GCNGC 5 cut(s) 22, 271, 311, 384, 736
Sau3AI GATC 4 cut(s) 12, 150, 319, 722
SchI GAGTC 2 cut(s) 368, 912
ScrFI CCNGG 1 cut(s) 26
SfaNI GCATC 2 cut(s) 322, 558
SfoI GGCGCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SphI GCATGC 1 cut(s) 763
Sse9I AATT 7 cut(s) 351, 595, 665, 674, 729, 892, 927
SsiI CCGC 6 cut(s) 21, 102, 168, 329, 736, 907
SspDI GGCGCC 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 2 cut(s) 75, 141
TaqI TCGA 3 cut(s) 9, 84, 186
TasI AATT 7 cut(s) 351, 595, 665, 674, 729, 892, 927
TatI WGTACW 2 cut(s) 42, 608
TauI GCSGC 2 cut(s) 24, 738
TfiI GAWTC 3 cut(s) 95, 246, 575
Tru1I TTAA 5 cut(s) 594, 639, 717, 915, 952
Tru9I TTAA 5 cut(s) 594, 639, 717, 915, 952
TscAI CASTG 1 cut(s) 494
TseI GCWGC 3 cut(s) 270, 310, 383
TspDTI ATGAA 5 cut(s) 238, 515, 551, 572, 589
TspGWI ACGGA 3 cut(s) 244, 539, 884
TspRI CASTG 1 cut(s) 494
Tth111I GACNNNGTC 1 cut(s) 71
XapI RAATTY 2 cut(s) 665, 674
XceI RCATGY 1 cut(s) 763
XmiI GTMKAC 2 cut(s) 135, 797
XmnI GAANNNNTTC 2 cut(s) 579, 696
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.