MD07G1310300.v1.1

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003421
Physical Location & Seq
Reverse (-)
36504231 .. 36507299
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1310300.v1.1.491

Sequence Viewer

Length: 978 bp
ATGTGGTTAGAGATCATCTGCGGGCTGATAATATACCAGCTGTACAGGTGGTTCGTCTCCTCCGATGACGACGACGTCTTGGACCTGGAGACTTCTGATTCCAAGGCTCTCTTCTCCGTCGGTGACAGGCTTGCCAAGCTTTACGGCGGTAAGGTCTACGTTGGCCTTCGCATTCCCGATGCTGATACCGCTTCCCCTCAGACTATCGATTTGGTCCTCGTCTCCGAAGGGGAGGCAGCGGTCGTGAGTGTAAAGAATTTATCAGGATTAGTTTCAGTGAATGTGGATGGCAGCTGGGTTTGTGAGGGCTACAGTAGTAATCACAAGGCGCAGCACCTACCCGACCCTGTGTTGGAGACCAAAAAACAAGCTTCAATTCTTGAATCATATTTAGAACAAAGAGGAGTTGCTTTACCAAAAGGATATTTGTCTTGCAAAGTAATCCTTTCCAATCCAAAAGTTTGTACCATTCAGTCCAGCAATTTTCCATCAGAAGTCGTAACCTATGACCAGTGGGTGCAGCTGAAACCAGAACCAAAAAACATGTTTTCTGGTTGGTTCAAAGGTGCCTTCCGTGGTGGAAAGAAAGAGATGCAAGAATCTATGCATCAGAAGCTTGATTCCATTCTTAGTACAGCTCCAATGTGGGATAGGTTGGAGCTCAAAGGTAGTAAGTATGTTCTTGGAGAATTTCTAGAATTCAAGGGTAAGCAGGAAGATGTTCAGGATTTGAAATGTATCAAAAGATCAAAAATTGGCCGCCTTGTAGTCCAAAAGACGAGCATGCTTGGATTTGCCCCTTCTAGGCTTCAAGTTCTGTACTCTCCTCGTGATTATCGGAGCGAAGGAGCTTCAGCTTCTGAGTGGATGGAGGTAACTGTAAGGTCTAGTACAGAAGTCCTCTTCCAGCCAGAAAATTCTTCTAGAGTACGCAAGTTTAAGCTTTCTTCGATCGTCTCTATGTCACTGAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

326

Amino Acids

36.35

Weight (kDa)

7.56

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 39 - 135 3.5e-11 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 566
AccI GTMKAC 1 cut(s) 156
AciI CCGC 5 cut(s) 21, 147, 189, 239, 760
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 4 cut(s) 256, 689, 698, 916
AcuI CTGAAG 1 cut(s) 837
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 6 cut(s) 44, 466, 634, 821, 892, 930
AfiI CCNNNNNNNGG 2 cut(s) 352, 804
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 6 cut(s) 375, 383, 562, 703, 733, 812
AjnI CCWGG 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 5 cut(s) 61, 83, 226, 350, 961
AlwNI CAGNNNCTG 1 cut(s) 860
AoxI GGCC 2 cut(s) 163, 757
ApeKI GCWGC 4 cut(s) 236, 291, 331, 520
ApoI RAATTY 4 cut(s) 256, 689, 698, 916
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 82, 214
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 2 cut(s) 82, 214
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 1 cut(s) 663
BauI CACGAG 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 663
BbvI GCAGC 4 cut(s) 248, 303, 343, 532
BccI CCATC 3 cut(s) 281, 496, 862
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 5 cut(s) 61, 83, 226, 350, 961
BfaI CTAG 4 cut(s) 695, 804, 888, 924
BfmI CTRYAG 1 cut(s) 310
BisI GCNGC 5 cut(s) 237, 292, 332, 521, 760
BlsI GCNGC 5 cut(s) 238, 293, 333, 522, 761
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 2 cut(s) 82, 214
BmgT120I GGNCC 2 cut(s) 82, 214
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 3 cut(s) 169, 582, 616
BpmI CTGGAG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 102, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 804
Bse1I ACTGG 1 cut(s) 511
BseBI CCWGG 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 102, 574
BseGI GGATG 2 cut(s) 292, 873
BseLI CCNNNNNNNGG 2 cut(s) 352, 804
BseMII CTCAG 3 cut(s) 212, 852, 959
BseNI ACTGG 1 cut(s) 511
BseRI GAGGAG 3 cut(s) 49, 417, 816
BseXI GCAGC 4 cut(s) 248, 303, 343, 532
BseYI CCCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 539
Bsh1285I CGRYCG 2 cut(s) 243, 954
BshFI GGCC 2 cut(s) 165, 759
BshNI GGYRCC 1 cut(s) 566
BshVI ATCGAT 1 cut(s) 207
BsiEI CGRYCG 2 cut(s) 243, 954
BsiHKAI GWGCWC 1 cut(s) 663
BslI CCNNNNNNNGG 2 cut(s) 352, 804
BsmAI GTCTC 5 cut(s) 61, 83, 226, 350, 961
BsmBI CGTCTC 3 cut(s) 61, 226, 961
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 2 cut(s) 165, 759
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 663
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 3 cut(s) 12, 746, 951
BspACI CCGC 5 cut(s) 21, 147, 189, 239, 760
BspANI GGCC 2 cut(s) 165, 759
BspCNI CTCAG 3 cut(s) 211, 853, 960
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 2 cut(s) 102, 574
BssMI GATC 3 cut(s) 12, 746, 951
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 828
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 2 cut(s) 314, 880
Bst6I CTCTTC 2 cut(s) 116, 908
BstACI GRCGYC 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 3 cut(s) 23, 132, 785
BstDEI CTNAG 4 cut(s) 198, 629, 861, 968
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 2 cut(s) 292, 873
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 3 cut(s) 15, 749, 954
BstMAI GTCTC 5 cut(s) 61, 83, 226, 350, 961
BstMBI GATC 3 cut(s) 12, 746, 951
BstMCI CGRYCG 2 cut(s) 243, 954
BstMWI GCNNNNNNNGC 3 cut(s) 136, 188, 613
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 2 cut(s) 547, 787
BstSCI CCNGG 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 310
BstV1I GCAGC 4 cut(s) 248, 303, 343, 532
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 2 cut(s) 165, 759
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 574
BtsCI GGATG 2 cut(s) 292, 873
BtsIMutI CAGTG 3 cut(s) 282, 518, 965
Cac8I GCNNGC 3 cut(s) 23, 132, 785
CaiI CAGNNNCTG 1 cut(s) 860
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 82, 214
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 6 cut(s) 43, 465, 633, 820, 891, 929
CviAII CATG 2 cut(s) 544, 784
CviQI GTAC 6 cut(s) 43, 465, 633, 820, 891, 929
DdeI CTNAG 4 cut(s) 198, 629, 861, 968
DpnI GATC 3 cut(s) 14, 748, 953
DpnII GATC 3 cut(s) 12, 746, 951
DraIII CACNNNGTG 1 cut(s) 971
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 757
Eam1104I CTCTTC 2 cut(s) 116, 908
EarI CTCTTC 2 cut(s) 116, 908
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 663
Eco31I GGTCTC 1 cut(s) 350
Eco47I GGWCC 2 cut(s) 82, 214
Eco53kI GAGCTC 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 837
EcoICRI GAGCTC 1 cut(s) 661
EcoRI GAATTC 1 cut(s) 698
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 102
Esp3I CGTCTC 3 cut(s) 61, 226, 961
FaeI CATG 2 cut(s) 547, 787
FaiI YATR 8 cut(s) 34, 388, 507, 545, 605, 678, 785, 962
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 429, 461
FatI CATG 2 cut(s) 543, 783
FauI CCCGC 1 cut(s) 14
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 5 cut(s) 237, 292, 332, 521, 760
FokI GGATG 2 cut(s) 299, 880
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 5 cut(s) 237, 292, 332, 521, 760
FspBI CTAG 4 cut(s) 695, 804, 888, 924
GlaI GCGC 1 cut(s) 330
GluI GCNGC 5 cut(s) 237, 292, 332, 521, 760
GsaI CCCAGC 1 cut(s) 298
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 2 cut(s) 165, 759
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 547, 787
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 4 cut(s) 137, 369, 614, 941
HinfI GANTC 4 cut(s) 98, 383, 599, 620
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 8 cut(s) 64, 97, 201, 226, 493, 612, 840, 862
Hpy188III TCNNGA 8 cut(s) 176, 244, 264, 380, 695, 725, 830, 924
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 3 cut(s) 74, 77, 122
HpyAV CCTTC 5 cut(s) 176, 221, 580, 810, 839
HpyCH4III ACNGT 2 cut(s) 314, 880
HpyCH4IV ACGT 2 cut(s) 75, 159
HpyCH4V TGCA 4 cut(s) 435, 520, 595, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 188, 613
HpyF3I CTNAG 4 cut(s) 198, 629, 861, 968
HpySE526I ACGT 2 cut(s) 75, 159
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 547, 787
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 3 cut(s) 12, 746, 951
LmnI GCTCC 4 cut(s) 643, 658, 840, 848
Lsp1109I GCAGC 4 cut(s) 248, 303, 343, 532
LweI GCATC 3 cut(s) 169, 582, 616
MaeI CTAG 4 cut(s) 695, 804, 888, 924
MaeII ACGT 2 cut(s) 75, 159
MaeIII GTNAC 4 cut(s) 122, 499, 874, 963
MalI GATC 3 cut(s) 14, 748, 953
MboI GATC 3 cut(s) 12, 746, 951
MboII GAAGA 5 cut(s) 103, 728, 895, 912, 939
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 7 cut(s) 256, 375, 481, 689, 698, 753, 916
MmeI TCCRAC 2 cut(s) 333, 636
MnlI CCTC 9 cut(s) 70, 207, 226, 227, 298, 395, 837, 865, 911
Mph1103I ATGCAT 1 cut(s) 609
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 2 cut(s) 939, 976
MspA1I CMGCKG 4 cut(s) 40, 239, 294, 523
MspR9I CCNGG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 3 cut(s) 136, 188, 613
NdeII GATC 3 cut(s) 12, 746, 951
NlaIII CATG 2 cut(s) 547, 787
NlaIV GGNNCC 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 122, 963
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 2 cut(s) 547, 787
PaeI GCATGC 1 cut(s) 787
PciI ACATGT 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 4 cut(s) 98, 383, 599, 620
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 5 cut(s) 238, 293, 333, 522, 761
Ple19I CGATCG 1 cut(s) 954
PscI ACATGT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 2 cut(s) 82, 214
PsrI GAACNNNNNNTAC 2 cut(s) 35, 67
PstNI CAGNNNCTG 1 cut(s) 860
PsyI GACNNNGTC 1 cut(s) 74
PvuI CGATCG 1 cut(s) 954
PvuII CAGCTG 3 cut(s) 40, 294, 523
RsaI GTAC 6 cut(s) 44, 466, 634, 821, 892, 930
RsaNI GTAC 6 cut(s) 43, 465, 633, 820, 891, 929
SacI GAGCTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 939, 976
SatI GCNGC 5 cut(s) 237, 292, 332, 521, 760
Sau3AI GATC 3 cut(s) 12, 746, 951
Sau96I GGNCC 2 cut(s) 82, 214
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 663
SfaNI GCATC 3 cut(s) 169, 582, 616
SfcI CTRYAG 1 cut(s) 310
SinI GGWCC 2 cut(s) 82, 214
SphI GCATGC 1 cut(s) 787
Sse9I AATT 7 cut(s) 256, 375, 481, 689, 698, 753, 916
SsiI CCGC 5 cut(s) 21, 147, 189, 239, 760
SspMI CTAG 4 cut(s) 695, 804, 888, 924
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 314, 880
TaiI ACGT 2 cut(s) 78, 162
TaqI TCGA 2 cut(s) 207, 950
TasI AATT 7 cut(s) 256, 375, 481, 689, 698, 753, 916
TatI WGTACW 4 cut(s) 42, 632, 819, 890
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 4 cut(s) 98, 383, 599, 620
Tru1I TTAA 2 cut(s) 939, 976
Tru9I TTAA 2 cut(s) 939, 976
TscAI CASTG 3 cut(s) 282, 518, 972
TseFI GTSAC 2 cut(s) 122, 963
TseI GCWGC 4 cut(s) 236, 291, 331, 520
Tsp45I GTSAC 2 cut(s) 122, 963
TspGWI ACGGA 2 cut(s) 106, 563
TspRI CASTG 3 cut(s) 282, 518, 972
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 82, 214
XapI RAATTY 4 cut(s) 256, 689, 698, 916
XbaI TCTAGA 2 cut(s) 694, 923
XceI RCATGY 2 cut(s) 547, 787
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 4 cut(s) 695, 804, 888, 924
ZraI GACGTC 1 cut(s) 76
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.