RLG00000032877

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003421
Physical Location & Seq
Forward (+)
20258636 .. 20260173
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032877

Sequence Viewer

Length: 951 bp
ATGCTCTTACACGCCGGTCACATACAGACCCTCATAATCTCCTCAGCTGAAGCAGCTCAACAAGTCTTGAGAGATAATGACATCCTCTGCTGCAGTAGACCCGTCTCCACGGGGTTTCAAAAAATCACTTACAACTATCTAGACATGGCATTTGAGCCATACGGTAAGTATTGGCGAGAGATAAGAAAGATATGTGGGCTTGAGCTTTTCAGTGTGAAAAGAGTACACTCGTATAAGTCCATTAGGGAAGACGAAGTGGCTAAGATGATTGATTCAATCTCTAACAACTTCGCTTCCTCTTCTTCTTCTGTTCATCTCACTGACAAGTTGTTTGCTCTCATGGGAAGCATAATATTCAGGATTGTTTTCGAGATCAGTTTTGAGAAGAGTGATTTCGAGCATGACAGATTTCATCAACTGCTTCAAGATACAACAATCATGATTGTAGGCTTCTCTGCAGCTGATTATTTTCCTTCTCGCGTCGGATTCAAATCCTTAAAGGCTCAACGCCAGCCGAGACCCACAGCTGTATCCGGTAATCAATCGATGGGTCTCAGATTATGGGCTGGAAGCACCGATGAAGACGAAGATGAAGCTCAGATTCAGACCAAATCTGAGATGATTGCTATCCTGGGGAAGGGTTTCTATGGAGTCGGAGAGGCTTTCTTCGCTTCCGGAGTGGAATTTCACCAAGATTTTGAGGAACTTCATAGGAATGCTCATCTGGAGATGATTAAACTCTCGAAGAAGTTTGCAGCATTAAAGAGGAAGGTCTCTGGAGAACTTGATCCTTTTTGTCAGCATGTGATTGATGATCATCTCAAGCATGGAAAGACACAACAAGAGCATGAAGACATAGTTGATGTACTGATTAAATTTGCAAAGAAGCCTAGTTCTGGAACTGCTCAGCTTGGTCATAATAACATTAAGGCTGTCATCATGGTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

317

Amino Acids

35.7

Weight (kDa)

6.54

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 157 2.9e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 97
AccII CGCG 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 674
AclWI GGATC 1 cut(s) 782
AcsI RAATTY 2 cut(s) 683, 875
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 2 cut(s) 225, 867
AfiI CCNNNNNNNGG 2 cut(s) 637, 896
AgsI TTSAA 4 cut(s) 119, 276, 425, 490
AjnI CCWGG 1 cut(s) 630
AluBI AGCT 8 cut(s) 47, 56, 205, 461, 527, 596, 910, 948
AluI AGCT 8 cut(s) 47, 56, 205, 461, 527, 596, 910, 948
Alw26I GTCTC 4 cut(s) 109, 511, 557, 778
AlwI GGATC 1 cut(s) 782
Aor13HI TCCGGA 1 cut(s) 674
ApeKI GCWGC 4 cut(s) 53, 90, 458, 755
ApoI RAATTY 2 cut(s) 683, 875
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 314, 346
Asp700I GAANNNNTTC 1 cut(s) 641
AsuHPI GGTGA 1 cut(s) 680
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BbsI GAAGAC 3 cut(s) 255, 588, 858
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 4 cut(s) 65, 77, 470, 767
BccI CCATC 1 cut(s) 541
BciT130I CCWGG 1 cut(s) 632
BciVI GTATCC 1 cut(s) 541
BclI TGATCA 1 cut(s) 814
BcoDI GTCTC 4 cut(s) 109, 511, 557, 778
BfaI CTAG 2 cut(s) 140, 891
BfmI CTRYAG 2 cut(s) 91, 456
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 4 cut(s) 54, 91, 459, 756
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 4 cut(s) 55, 92, 460, 757
Bme1390I CCNGG 1 cut(s) 632
BmrFI CCNGG 1 cut(s) 632
BpiI GAAGAC 3 cut(s) 255, 588, 858
BpmI CTGGAG 2 cut(s) 746, 798
Bpu10I CCTNAGC 1 cut(s) 43
Bpu1102I GCTNAGC 1 cut(s) 906
BpuEI CTTGAG 3 cut(s) 88, 221, 806
Bsa29I ATCGAT 1 cut(s) 545
BsaBI GATNNNNATC 3 cut(s) 490, 626, 816
BsaI GGTCTC 3 cut(s) 511, 557, 778
BsaJI CCNNGG 2 cut(s) 108, 631
BsaWI WCCGGW 2 cut(s) 533, 674
Bsc4I CCNNNNNNNGG 2 cut(s) 637, 896
Bse118I RCCGGY 1 cut(s) 14
Bse8I GATNNNNATC 3 cut(s) 490, 626, 816
BseAI TCCGGA 1 cut(s) 674
BseBI CCWGG 1 cut(s) 632
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 108, 631
BseGI GGATG 1 cut(s) 81
BseJI GATNNNNATC 3 cut(s) 490, 626, 816
BseLI CCNNNNNNNGG 2 cut(s) 637, 896
BseMII CTCAG 5 cut(s) 57, 568, 606, 611, 920
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 4 cut(s) 65, 77, 470, 767
Bsh1236I CGCG 1 cut(s) 480
BshVI ATCGAT 1 cut(s) 545
BsiSI CCGG 3 cut(s) 15, 534, 675
BslI CCNNNNNNNGG 2 cut(s) 637, 896
BsmAI GTCTC 4 cut(s) 109, 511, 557, 778
BsmBI CGTCTC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 721
Bso31I GGTCTC 3 cut(s) 511, 557, 778
Bsp13I TCCGGA 1 cut(s) 674
Bsp143I GATC 3 cut(s) 372, 787, 814
Bsp1720I GCTNAGC 1 cut(s) 906
BspCNI CTCAG 5 cut(s) 56, 567, 607, 610, 919
BspDI ATCGAT 1 cut(s) 545
BspEI TCCGGA 1 cut(s) 674
BspFNI CGCG 1 cut(s) 480
BspHI TCATGA 1 cut(s) 438
BspMAI CTGCAG 2 cut(s) 95, 460
BspPI GGATC 1 cut(s) 782
BspTNI GGTCTC 3 cut(s) 511, 557, 778
BsrFI RCCGGY 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 108, 631
BssMI GATC 3 cut(s) 372, 787, 814
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 2 cut(s) 304, 380
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 6 cut(s) 43, 261, 554, 597, 615, 906
BstDSI CCRYGG 1 cut(s) 108
BstENI CCTNNNNNAGG 1 cut(s) 635
BstF5I GGATG 1 cut(s) 81
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 3 cut(s) 375, 790, 817
BstMAI GTCTC 4 cut(s) 109, 511, 557, 778
BstMBI GATC 3 cut(s) 372, 787, 814
BstMWI GCNNNNNNNGC 2 cut(s) 53, 668
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 1 cut(s) 630
BstSFI CTRYAG 2 cut(s) 91, 456
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 4 cut(s) 65, 77, 470, 767
BstV2I GAAGAC 3 cut(s) 255, 588, 858
Bsu15I ATCGAT 1 cut(s) 545
BsuI GTATCC 1 cut(s) 541
BsuTUI ATCGAT 1 cut(s) 545
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 2 cut(s) 217, 318
Cac8I GCNNGC 1 cut(s) 512
CciI TCATGA 1 cut(s) 438
Cfr10I RCCGGY 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 1 cut(s) 469
Csp6I GTAC 2 cut(s) 224, 866
CviAII CATG 8 cut(s) 145, 340, 401, 439, 803, 827, 848, 940
CviQI GTAC 2 cut(s) 224, 866
DdeI CTNAG 6 cut(s) 43, 261, 554, 597, 615, 906
DpnI GATC 3 cut(s) 374, 789, 816
DpnII GATC 3 cut(s) 372, 787, 814
Eam1104I CTCTTC 2 cut(s) 304, 380
EarI CTCTTC 2 cut(s) 304, 380
Eco31I GGTCTC 3 cut(s) 511, 557, 778
Eco57I CTGAAG 1 cut(s) 69
EcoNI CCTNNNNNAGG 1 cut(s) 635
EcoRII CCWGG 1 cut(s) 630
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 8 cut(s) 148, 343, 404, 442, 806, 830, 851, 943
FatI CATG 8 cut(s) 144, 339, 400, 438, 802, 826, 847, 939
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 97
Fnu4HI GCNGC 4 cut(s) 54, 91, 459, 756
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 4 cut(s) 54, 91, 459, 756
FspBI CTAG 2 cut(s) 140, 891
GluI GCNGC 4 cut(s) 54, 91, 459, 756
GsuI CTGGAG 2 cut(s) 746, 798
HapII CCGG 3 cut(s) 15, 534, 675
HgaI GACGC 1 cut(s) 469
Hin1II CATG 8 cut(s) 148, 343, 404, 442, 806, 830, 851, 943
HinfI GANTC 4 cut(s) 272, 486, 601, 651
HpaII CCGG 3 cut(s) 15, 534, 675
HphI GGTGA 1 cut(s) 680
Hpy166II GTNNAC 2 cut(s) 98, 226
Hpy188I TCNGA 6 cut(s) 485, 557, 600, 606, 616, 656
Hpy8I GTNNAC 2 cut(s) 98, 226
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 3 cut(s) 483, 631, 763
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 4 cut(s) 93, 458, 755, 881
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 668
HpyF3I CTNAG 6 cut(s) 43, 261, 554, 597, 615, 906
Hsp92II CATG 8 cut(s) 148, 343, 404, 442, 806, 830, 851, 943
Kpn2I TCCGGA 1 cut(s) 674
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 3 cut(s) 372, 787, 814
Lsp1109I GCAGC 4 cut(s) 65, 77, 470, 767
MaeI CTAG 2 cut(s) 140, 891
MaeIII GTNAC 1 cut(s) 17
MalI GATC 3 cut(s) 374, 789, 816
MboI GATC 3 cut(s) 372, 787, 814
MluCI AATT 2 cut(s) 683, 875
MlyI GAGTC 1 cut(s) 660
MmeI TCCRAC 2 cut(s) 463, 634
MnlI CCTC 7 cut(s) 41, 52, 95, 307, 652, 694, 759
MroI TCCGGA 1 cut(s) 674
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 5 cut(s) 497, 735, 761, 873, 927
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 3 cut(s) 47, 461, 527
MspI CCGG 3 cut(s) 15, 534, 675
MspR9I CCNGG 1 cut(s) 632
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 53, 668
NdeII GATC 3 cut(s) 372, 787, 814
NlaIII CATG 8 cut(s) 148, 343, 404, 442, 806, 830, 851, 943
NmeAIII GCCGAG 1 cut(s) 540
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 806
PagI TCATGA 1 cut(s) 438
PctI GAATGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 3 cut(s) 272, 486, 601
PkrI GCNGC 4 cut(s) 55, 92, 460, 757
PleI GAGTC 1 cut(s) 659
PpsI GAGTC 1 cut(s) 659
Psp6I CCWGG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 630
PstI CTGCAG 2 cut(s) 95, 460
PvuII CAGCTG 3 cut(s) 47, 461, 527
RsaI GTAC 2 cut(s) 225, 867
RsaNI GTAC 2 cut(s) 224, 866
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 5 cut(s) 497, 735, 761, 873, 927
SatI GCNGC 4 cut(s) 54, 91, 459, 756
Sau3AI GATC 3 cut(s) 372, 787, 814
SchI GAGTC 1 cut(s) 660
ScrFI CCNGG 1 cut(s) 632
SetI ASST 9 cut(s) 49, 58, 207, 463, 529, 598, 774, 912, 950
SfcI CTRYAG 2 cut(s) 91, 456
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 3 cut(s) 67, 200, 821
SmoI CTYRAG 3 cut(s) 67, 200, 821
Sse9I AATT 2 cut(s) 683, 875
SspI AATATT 1 cut(s) 354
SspMI CTAG 2 cut(s) 140, 891
StyD4I CCNGG 1 cut(s) 630
TaaI ACNGT 1 cut(s) 164
TaqI TCGA 4 cut(s) 369, 396, 545, 743
TasI AATT 2 cut(s) 683, 875
TatI WGTACW 2 cut(s) 223, 865
TfiI GAWTC 3 cut(s) 272, 486, 601
Tru1I TTAA 5 cut(s) 497, 735, 761, 873, 927
Tru9I TTAA 5 cut(s) 497, 735, 761, 873, 927
TscAI CASTG 2 cut(s) 217, 325
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 4 cut(s) 53, 90, 458, 755
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 6 cut(s) 302, 401, 594, 606, 698, 864
TspRI CASTG 2 cut(s) 217, 325
XagI CCTNNNNNAGG 1 cut(s) 635
XapI RAATTY 2 cut(s) 683, 875
XbaI TCTAGA 1 cut(s) 139
XceI RCATGY 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 97
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 2 cut(s) 140, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.