RLG00000008658

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003421
Physical Location & Seq
Reverse (-)
34336893 .. 34337438
546 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008658

Sequence Viewer

Length: 462 bp
ATGTCCGTTCCAGATCCCCCATTCACTCTGCTAAAATCGCAGTATTCATCTGCCAGTCCAACCCCCAATTTTCTAGGGTTAGAAATCATCTGCAGGCTGGTGATTTACAGACAGTACAAGTTGATCTTCTCCGATGGCAATGACGTCTTGGGTGTCGAGACCTCTAATTCTACTGCTCTCTTCGCCATCGCTGACAGGCTCGCTAAGCTCTATGATGGCGGCAAGGTCTCCGTTGGCCTCCGGATCCCCGACGCCGATACCGCTTCCCCTAAGCAAATCGATTTGGTTCTACTCAACAAAGGGGAGGCCGTGGTGATAACCGTCAAGAACTTGTCAAGATTCATCTCTGTCAAGCCCGATGGCAGCTGGGTTTCTGAGAGCATCACTAAGCACAAAGCAGACCTGCATCCTGATCCTGTCAGTCACTCCAACTCTTCACTCTTCTCTATGCCACCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

154

Amino Acids

16.57

Weight (kDa)

6.82

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 62 - 143 1.8e-06 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 411
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 2 cut(s) 219, 261
AclWI GGATC 4 cut(s) 8, 238, 251, 407
AcyI GRCGYC 2 cut(s) 144, 252
AfaI GTAC 1 cut(s) 116
AluBI AGCT 2 cut(s) 208, 366
AluI AGCT 2 cut(s) 208, 366
Alw26I GTCTC 2 cut(s) 152, 232
AlwI GGATC 4 cut(s) 8, 238, 251, 407
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 2 cut(s) 235, 306
ApeKI GCWGC 1 cut(s) 363
AsuHPI GGTGA 2 cut(s) 112, 325
BamHI GGATCC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 375
BccI CCATC 4 cut(s) 128, 194, 209, 353
BceAI ACGGC 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 152, 232
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 411
BisI GCNGC 2 cut(s) 220, 364
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 2 cut(s) 221, 365
BmiI GGNNCC 1 cut(s) 245
BmsI GCATC 2 cut(s) 390, 415
Bpu10I CCTNAGC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 204
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 2 cut(s) 144, 252
BsaI GGTCTC 2 cut(s) 152, 232
BsaJI CCNNGG 1 cut(s) 309
BsaWI WCCGGW 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 145
BseAI TCCGGA 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 1 cut(s) 406
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 375
BseYI CCCAGC 1 cut(s) 366
BshFI GGCC 2 cut(s) 237, 308
BshVI ATCGAT 1 cut(s) 279
BsiSI CCGG 1 cut(s) 241
BsmAI GTCTC 2 cut(s) 152, 232
BsnI GGCC 2 cut(s) 237, 308
Bso31I GGTCTC 2 cut(s) 152, 232
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 4 cut(s) 13, 123, 243, 412
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 2 cut(s) 219, 261
BspANI GGCC 2 cut(s) 237, 308
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 245
BspMAI CTGCAG 1 cut(s) 95
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 4 cut(s) 8, 238, 251, 407
BspTNI GGTCTC 2 cut(s) 152, 232
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 13, 123, 243, 412
BssNI GRCGYC 2 cut(s) 144, 252
Bst4CI ACNGT 2 cut(s) 114, 322
Bst6I CTCTTC 3 cut(s) 185, 439, 446
BstACI GRCGYC 2 cut(s) 144, 252
BstC8I GCNNGC 2 cut(s) 95, 201
BstDEI CTNAG 4 cut(s) 204, 270, 375, 387
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 406
BstKTI GATC 4 cut(s) 16, 126, 246, 415
BstMAI GTCTC 2 cut(s) 152, 232
BstMBI GATC 4 cut(s) 13, 123, 243, 412
BstMWI GCNNNNNNNGC 4 cut(s) 37, 182, 205, 260
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 13, 243
BstYI RGATCY 2 cut(s) 13, 243
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 2 cut(s) 237, 308
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 1 cut(s) 309
BtgZI GCGATG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 406
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 95, 201
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 115
CviJI RGCY 7 cut(s) 97, 199, 208, 237, 308, 355, 366
CviKI_1 RGCY 7 cut(s) 97, 199, 208, 237, 308, 355, 366
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 4 cut(s) 204, 270, 375, 387
DpnI GATC 4 cut(s) 15, 125, 245, 414
DpnII GATC 4 cut(s) 13, 123, 243, 412
Eam1104I CTCTTC 3 cut(s) 185, 439, 446
EarI CTCTTC 3 cut(s) 185, 439, 446
Eco31I GGTCTC 2 cut(s) 152, 232
FaiI YATR 2 cut(s) 213, 449
FalI AAGNNNNNCTT 2 cut(s) 110, 142
Fnu4HI GCNGC 2 cut(s) 220, 364
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 2 cut(s) 220, 364
FspBI CTAG 1 cut(s) 74
GluI GCNGC 2 cut(s) 220, 364
GsaI CCCAGC 1 cut(s) 370
HaeIII GGCC 2 cut(s) 237, 308
HapII CCGG 1 cut(s) 241
HgaI GACGC 1 cut(s) 260
Hin1I GRCGYC 2 cut(s) 144, 252
HinfI GANTC 1 cut(s) 339
HpaII CCGG 1 cut(s) 241
HphI GGTGA 2 cut(s) 112, 325
Hpy188I TCNGA 2 cut(s) 133, 376
Hpy188III TCNNGA 6 cut(s) 11, 157, 241, 325, 336, 410
Hpy99I CGWCG 1 cut(s) 254
HpyCH4III ACNGT 2 cut(s) 114, 322
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 2 cut(s) 93, 406
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 182, 205, 260
HpyF3I CTNAG 4 cut(s) 204, 270, 375, 387
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 2 cut(s) 144, 252
Kpn2I TCCGGA 1 cut(s) 240
Kzo9I GATC 4 cut(s) 13, 123, 243, 412
Lsp1109I GCAGC 1 cut(s) 375
LweI GCATC 2 cut(s) 390, 415
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 422
MalI GATC 4 cut(s) 15, 125, 245, 414
MboI GATC 4 cut(s) 13, 123, 243, 412
MboII GAAGA 4 cut(s) 118, 172, 426, 433
MflI RGATCY 2 cut(s) 13, 243
MluCI AATT 2 cut(s) 67, 166
MmeI TCCRAC 2 cut(s) 83, 453
MnlI CCTC 3 cut(s) 172, 248, 298
MroI TCCGGA 1 cut(s) 240
MseI TTAA 1 cut(s) 460
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 1 cut(s) 241
MwoI GCNNNNNNNGC 4 cut(s) 37, 182, 205, 260
NdeII GATC 4 cut(s) 13, 123, 243, 412
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 339
PkrI GCNGC 2 cut(s) 221, 365
PspFI CCCAGC 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 95
PsuI RGATCY 2 cut(s) 13, 243
PvuII CAGCTG 1 cut(s) 366
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SaqAI TTAA 1 cut(s) 460
SatI GCNGC 2 cut(s) 220, 364
Sau3AI GATC 4 cut(s) 13, 123, 243, 412
SetI ASST 6 cut(s) 147, 164, 210, 228, 368, 405
SfaNI GCATC 2 cut(s) 390, 415
SfcI CTRYAG 1 cut(s) 91
Sse9I AATT 2 cut(s) 67, 166
SsiI CCGC 2 cut(s) 219, 261
SspMI CTAG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 114, 322
TaiI ACGT 1 cut(s) 147
TaqI TCGA 2 cut(s) 156, 279
TasI AATT 2 cut(s) 67, 166
TatI WGTACW 1 cut(s) 114
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 1 cut(s) 460
Tru9I TTAA 1 cut(s) 460
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 1 cut(s) 363
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 36, 331
TspGWI ACGGA 1 cut(s) 220
XspI CTAG 1 cut(s) 74
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.