MD01G1237700.v1.1

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003421
Physical Location & Seq
Reverse (-)
32451759 .. 32454645
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1237700.v1.1.491

Sequence Viewer

Length: 978 bp
ATGTGGTTTGAGATCATCTGCGGGCTGATAATATACAAGTTTTACAGGTGCTTCGTCTCCTCCGATGACGACGACGTCTTGGACGTGGAGACCTCTGATTCCAAGGCTCTCTTCTCCGTCGGCGACAGACTTGCCAAGCTTTACGGCGGCAAGGTCTACGTCGGCCTTCGCATTCCCGATGCTGATACCGCTTCCCCTCAGACCATCGATTTGGTCCTCGTCTCCAAAGGGGAGGCAGTGGTCGTGAGTGTAAAGAATTTATCAGGATTAGTTTCAGTGAATGCGGATGGCAGCTGGGTTTGTGAAAGCCACAGTAGTAATCACAAAGCGCACCACCTACCTGACCCTGTGTTGGAGACCCAAAAACAAGCTTCAATTCTTGAATCATATTTAGAACAAAGAGGGTGTGCTTTACCACAAGGATATTTGTCTTGGAAAGTAATACTTTCCCATCCAAAAGTTTGCACCACTCAGTCAAGCAATTTTCCATCAGAGGTTGTAACCTACGACCAGTTGATGCAACTGAAACCGGAACCAAAAAACATGTTTTCTGGTTGGATTAAGGGTGCCTTCCGTGGTGGAAAGAAGGAGATGCAAGAATCTATATATCAGAAGCTTGATTTCATTCTTAGTACGGCTCCAATGTGGGATAGGTTGGAGCTGGAAGGTAGTAAGTATGTTCTTGGAGAATTTCTGGAATTTAAGGGTAAGCAGGAAGATGTTCAAGCTTTGAAAGGTATCAAAAGATCAAAAATTGGCCGCCTTGTTGTCCAAAAGACAAGCATGTTTGGATTTGCCCCTTCAAGGCTTCAAGTTCTGTACTCTCCTCGTGATTATCGGAGCGAAGGAGCTTCAGCTTCCGAGTGGATGGAGGTAAATGTAAGGTCTAGTACAGAGGTCCTCTTCCAACCAGAAAATTCTTCTAAAGTCCGCAAGTTTAAGCTCTCTTCAGTCGTCTCTATGTCACTGAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

326

Amino Acids

36.34

Weight (kDa)

7.6

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 39 - 135 8.5e-12 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 566
AccI GTMKAC 1 cut(s) 156
AciI CCGC 6 cut(s) 21, 147, 189, 284, 760, 931
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 4 cut(s) 256, 689, 698, 916
AcuI CTGAAG 2 cut(s) 837, 933
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 3 cut(s) 634, 821, 892
AfiI CCNNNNNNNGG 2 cut(s) 352, 804
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 6 cut(s) 375, 383, 725, 733, 804, 812
AjiI CACGTC 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 9 cut(s) 139, 294, 371, 616, 661, 728, 851, 857, 943
AluI AGCT 9 cut(s) 139, 294, 371, 616, 661, 728, 851, 857, 943
Alw26I GTCTC 5 cut(s) 61, 83, 226, 350, 961
AoxI GGCC 2 cut(s) 163, 757
ApeKI GCWGC 1 cut(s) 291
ApoI RAATTY 4 cut(s) 256, 689, 698, 916
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 214, 898
AvaII GGWCC 2 cut(s) 214, 898
BanI GGYRCC 1 cut(s) 566
BauI CACGAG 1 cut(s) 828
BbvI GCAGC 1 cut(s) 303
BccI CCATC 5 cut(s) 212, 281, 459, 496, 862
BceAI ACGGC 2 cut(s) 160, 651
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BcoDI GTCTC 5 cut(s) 61, 83, 226, 350, 961
BfaI CTAG 1 cut(s) 888
BisI GCNGC 3 cut(s) 148, 292, 760
BlsI GCNGC 3 cut(s) 149, 293, 761
Bme18I GGWCC 2 cut(s) 214, 898
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 214, 898
BmiI GGNNCC 3 cut(s) 534, 568, 639
BmsI GCATC 3 cut(s) 169, 507, 582
Bsa29I ATCGAT 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 2 cut(s) 83, 350
BsaJI CCNNGG 2 cut(s) 102, 574
BsaWI WCCGGW 1 cut(s) 529
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 804
Bse1I ACTGG 1 cut(s) 511
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 102, 574
BseGI GGATG 3 cut(s) 292, 451, 873
BseLI CCNNNNNNNGG 2 cut(s) 352, 804
BseMII CTCAG 3 cut(s) 212, 485, 959
BseNI ACTGG 1 cut(s) 511
BseRI GAGGAG 2 cut(s) 49, 816
BseXI GCAGC 1 cut(s) 303
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 2 cut(s) 165, 759
BshNI GGYRCC 1 cut(s) 566
BshVI ATCGAT 1 cut(s) 207
BsiSI CCGG 1 cut(s) 530
BslI CCNNNNNNNGG 2 cut(s) 352, 804
BsmAI GTCTC 5 cut(s) 61, 83, 226, 350, 961
BsmBI CGTCTC 3 cut(s) 61, 226, 961
BsmI GAATGC 2 cut(s) 171, 286
BsnI GGCC 2 cut(s) 165, 759
Bso31I GGTCTC 2 cut(s) 83, 350
Bsp143I GATC 2 cut(s) 12, 746
BspACI CCGC 6 cut(s) 21, 147, 189, 284, 760, 931
BspANI GGCC 2 cut(s) 165, 759
BspCNI CTCAG 3 cut(s) 211, 484, 960
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 534, 568, 639
BspT107I GGYRCC 1 cut(s) 566
BspTNI GGTCTC 2 cut(s) 83, 350
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 2 cut(s) 102, 574
BssMI GATC 2 cut(s) 12, 746
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 828
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 3 cut(s) 116, 908, 952
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 4 cut(s) 198, 471, 629, 968
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 3 cut(s) 292, 451, 873
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 15, 749
BstMAI GTCTC 5 cut(s) 61, 83, 226, 350, 961
BstMBI GATC 2 cut(s) 12, 746
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 2 cut(s) 547, 787
BstV1I GCAGC 1 cut(s) 303
BstXI CCANNNNNNTGG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 2 cut(s) 165, 759
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 574
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 3 cut(s) 292, 451, 873
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 282, 965
Cac8I GCNNGC 1 cut(s) 23
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 214, 898
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 3 cut(s) 633, 820, 891
CviAII CATG 2 cut(s) 544, 784
CviQI GTAC 3 cut(s) 633, 820, 891
DdeI CTNAG 4 cut(s) 198, 471, 629, 968
DpnI GATC 2 cut(s) 14, 748
DpnII GATC 2 cut(s) 12, 746
DraIII CACNNNGTG 1 cut(s) 971
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 757
Eam1104I CTCTTC 3 cut(s) 116, 908, 952
EarI CTCTTC 3 cut(s) 116, 908, 952
Eco130I CCWWGG 1 cut(s) 102
Eco31I GGTCTC 2 cut(s) 83, 350
Eco47I GGWCC 2 cut(s) 214, 898
Eco57I CTGAAG 2 cut(s) 837, 933
EcoO109I RGGNCCY 1 cut(s) 898
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
Esp3I CGTCTC 3 cut(s) 61, 226, 961
FaeI CATG 2 cut(s) 547, 787
FaiI YATR 8 cut(s) 34, 388, 545, 605, 607, 678, 785, 962
FalI AAGNNNNNCTT 6 cut(s) 95, 127, 429, 461, 554, 586
FatI CATG 2 cut(s) 543, 783
FauI CCCGC 1 cut(s) 14
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 148, 292, 760
FokI GGATG 3 cut(s) 299, 438, 880
Fsp4HI GCNGC 3 cut(s) 148, 292, 760
FspBI CTAG 1 cut(s) 888
GlaI GCGC 1 cut(s) 330
GluI GCNGC 3 cut(s) 148, 292, 760
GsaI CCCAGC 1 cut(s) 298
HaeIII GGCC 2 cut(s) 165, 759
HapII CCGG 1 cut(s) 530
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 547, 787
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 4 cut(s) 137, 369, 614, 726
HinfI GANTC 3 cut(s) 98, 383, 599
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 7 cut(s) 64, 97, 201, 493, 612, 840, 862
Hpy188III TCNNGA 6 cut(s) 176, 244, 264, 380, 695, 830
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 4 cut(s) 74, 77, 122, 164
HpyAV CCTTC 6 cut(s) 176, 580, 580, 659, 810, 839
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 3 cut(s) 75, 84, 159
HpyCH4V TGCA 3 cut(s) 465, 520, 595
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 4 cut(s) 198, 471, 629, 968
HpySE526I ACGT 3 cut(s) 75, 84, 159
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 547, 787
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 2 cut(s) 12, 746
LmnI GCTCC 4 cut(s) 643, 658, 840, 848
Lsp1109I GCAGC 1 cut(s) 303
LweI GCATC 3 cut(s) 169, 507, 582
MaeI CTAG 1 cut(s) 888
MaeII ACGT 3 cut(s) 75, 84, 159
MaeIII GTNAC 2 cut(s) 499, 963
MalI GATC 2 cut(s) 14, 748
MboI GATC 2 cut(s) 12, 746
MboII GAAGA 5 cut(s) 103, 728, 895, 912, 939
MluCI AATT 7 cut(s) 256, 375, 481, 689, 698, 753, 916
MmeI TCCRAC 4 cut(s) 333, 536, 636, 931
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 4 cut(s) 561, 702, 939, 976
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 1 cut(s) 530
Mva1269I GAATGC 2 cut(s) 171, 286
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 2 cut(s) 12, 746
NlaIII CATG 2 cut(s) 547, 787
NlaIV GGNNCC 3 cut(s) 534, 568, 639
NmuCI GTSAC 1 cut(s) 963
NspI RCATGY 2 cut(s) 547, 787
PciI ACATGT 1 cut(s) 543
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 81
PctI GAATGC 2 cut(s) 171, 286
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 3 cut(s) 98, 383, 599
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 3 cut(s) 149, 293, 761
PpuMI RGGWCCY 1 cut(s) 898
PscI ACATGT 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 898
PspFI CCCAGC 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 534, 568, 639
PspPI GGNCC 2 cut(s) 214, 898
PspPPI RGGWCCY 1 cut(s) 898
PsyI GACNNNGTC 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 3 cut(s) 634, 821, 892
RsaNI GTAC 3 cut(s) 633, 820, 891
SaqAI TTAA 4 cut(s) 561, 702, 939, 976
SatI GCNGC 3 cut(s) 148, 292, 760
Sau3AI GATC 2 cut(s) 12, 746
Sau96I GGNCC 2 cut(s) 214, 898
SfaNI GCATC 3 cut(s) 169, 507, 582
SinI GGWCC 2 cut(s) 214, 898
Sse9I AATT 7 cut(s) 256, 375, 481, 689, 698, 753, 916
SsiI CCGC 6 cut(s) 21, 147, 189, 284, 760, 931
SspMI CTAG 1 cut(s) 888
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 3 cut(s) 78, 87, 162
TaqI TCGA 1 cut(s) 207
TasI AATT 7 cut(s) 256, 375, 481, 689, 698, 753, 916
TatI WGTACW 2 cut(s) 819, 890
TauI GCSGC 2 cut(s) 150, 762
TfiI GAWTC 3 cut(s) 98, 383, 599
Tru1I TTAA 4 cut(s) 561, 702, 939, 976
Tru9I TTAA 4 cut(s) 561, 702, 939, 976
TscAI CASTG 3 cut(s) 243, 282, 972
TseFI GTSAC 1 cut(s) 963
TseI GCWGC 1 cut(s) 291
Tsp45I GTSAC 1 cut(s) 963
TspDTI ATGAA 1 cut(s) 613
TspGWI ACGGA 2 cut(s) 106, 563
TspRI CASTG 3 cut(s) 243, 282, 972
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 214, 898
XapI RAATTY 4 cut(s) 256, 689, 698, 916
XceI RCATGY 2 cut(s) 547, 787
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 1 cut(s) 888
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.