RLG00000026037

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003421
Physical Location & Seq
Forward (+)
170784 .. 174901
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026037

Sequence Viewer

Length: 723 bp
ATGTGGTTCGAAATCATCTGCGGCCTGGTGATTTACAGACTGTACAAGTTGTTCTTCTCCGACGGCGACGACGTCTTGGATGTCGAGACCTCTGATTCTACTGCTCTCTTCGCCGTCGCCGACAGGCTCGCTAAGCTCTACGATGGCGCCAAGGTCTACGTTGGCCTCCGGATCCCCGACGCTGATACCGCTTCCCCTCAGCACATCGATTTGGTTCTCCTCAACAGAGGGGAGGCCGTGGTGATAGCCGTCAAGAACTTGTCAGGATTCATCTCCGTCAAGCCCGATGGCAGCTGGGTTTCTGAGAGCCTCACTAAACACAAAATAGAGCACCATCCTGATCCTCTGGCGGAGACAAAAAGACAAGCTTCAATTCTTGAGTCCTATCTTGAACAAAGAGGAGCTGCTTTACCAGAAGGATATTTGTCTTGCAAAGTAATTCTTTCCAATCCTAAAGTATGTACTATTCAGTCAAGCAGTTTCCCACCAGAAATCATTACTTATGACCAGTGGGTACAAATGAAACCAGAACCAAAAAGTATGTTTTCTGATTGGATAAAGGGCGCATTCCGTGGTGGAAAGAAAGAGATGCAAGAATCTATTCATCAGAAGCTTAATTTCATTCTTAGTTCAGCACCAATGTGGGATAGGTTTGTTCCCTCTACACCTTTTGCTGGTAAATATTGCATGGGAATTGGATGCCCATCTTTTAGGCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

241

Amino Acids

26.85

Weight (kDa)

6.22

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 37 - 144 7.7e-13 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 156
AccIII TCCGGA 1 cut(s) 168
AciI CCGC 3 cut(s) 21, 189, 350
AclWI GGATC 3 cut(s) 166, 179, 335
AcyI GRCGYC 2 cut(s) 72, 147
AfaI GTAC 3 cut(s) 44, 463, 516
AfiI CCNNNNNNNGG 1 cut(s) 674
AgsI TTSAA 2 cut(s) 372, 392
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 1 cut(s) 640
AluBI AGCT 5 cut(s) 136, 294, 368, 404, 613
AluI AGCT 5 cut(s) 136, 294, 368, 404, 613
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 80, 347
AlwI GGATC 3 cut(s) 166, 179, 335
Aor13HI TCCGGA 1 cut(s) 168
AoxI GGCC 4 cut(s) 22, 163, 234, 713
ApeKI GCWGC 2 cut(s) 291, 404
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 2 cut(s) 149, 566
AsuHPI GGTGA 2 cut(s) 40, 253
AsuII TTCGAA 1 cut(s) 9
BamHI GGATCC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 303, 391
BccI CCATC 4 cut(s) 137, 281, 342, 712
BceAI ACGGC 4 cut(s) 79, 98, 221, 233
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 80, 347
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 3 cut(s) 22, 292, 405
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 3 cut(s) 23, 293, 406
Bme1390I CCNGG 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 148, 173
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 579, 689
Bpu10I CCTNAGC 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 703
BsaHI GRCGYC 2 cut(s) 72, 147
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 150, 237, 571
BsaWI WCCGGW 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse1I ACTGG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 703
BseAI TCCGGA 1 cut(s) 168
BseBI CCWGG 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 3 cut(s) 150, 237, 571
BseGI GGATG 3 cut(s) 85, 334, 704
BseJI GATNNNNATC 1 cut(s) 703
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMII CTCAG 2 cut(s) 212, 294
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 2 cut(s) 209, 414
BseXI GCAGC 2 cut(s) 303, 391
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 4 cut(s) 24, 165, 236, 715
BshNI GGYRCC 1 cut(s) 146
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 2 cut(s) 80, 347
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 4 cut(s) 24, 165, 236, 715
Bso31I GGTCTC 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 333
Bsp13I TCCGGA 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 2 cut(s) 171, 340
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 3 cut(s) 21, 189, 350
BspANI GGCC 4 cut(s) 24, 165, 236, 715
BspCNI CTCAG 2 cut(s) 211, 295
BspDI ATCGAT 1 cut(s) 207
BspEI TCCGGA 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 148, 173
BspPI GGATC 3 cut(s) 166, 179, 335
BspT104I TTCGAA 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 3 cut(s) 150, 237, 571
BssMI GATC 2 cut(s) 171, 340
BssNI GRCGYC 2 cut(s) 72, 147
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 2 cut(s) 72, 147
BstAUI TGTACA 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 5 cut(s) 132, 198, 303, 626, 717
BstDSI CCRYGG 2 cut(s) 237, 571
BstF5I GGATG 3 cut(s) 85, 334, 704
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 566
BstKTI GATC 2 cut(s) 174, 343
BstMAI GTCTC 2 cut(s) 80, 347
BstMBI GATC 2 cut(s) 171, 340
BstMWI GCNNNNNNNGC 3 cut(s) 110, 133, 188
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 303, 391
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 4 cut(s) 24, 165, 236, 715
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 2 cut(s) 237, 571
BtsCI GGATG 3 cut(s) 85, 334, 704
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 2 cut(s) 149, 566
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 43, 462, 515
CviAII CATG 1 cut(s) 688
CviQI GTAC 3 cut(s) 43, 462, 515
DdeI CTNAG 5 cut(s) 132, 198, 303, 626, 717
DinI GGCGCC 1 cut(s) 148
DpnI GATC 2 cut(s) 173, 342
DpnII GATC 2 cut(s) 171, 340
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 715
Eco31I GGTCTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 1 cut(s) 691
FaiI YATR 4 cut(s) 460, 504, 542, 689
FalI AAGNNNNNCTT 6 cut(s) 38, 70, 352, 384, 426, 458
FatI CATG 1 cut(s) 687
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 22, 292, 405
FokI GGATG 3 cut(s) 92, 321, 711
Fsp4HI GCNGC 3 cut(s) 22, 292, 405
GlaI GCGC 2 cut(s) 148, 565
GluI GCNGC 3 cut(s) 22, 292, 405
GsaI CCCAGC 1 cut(s) 298
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 4 cut(s) 24, 165, 236, 715
HapII CCGG 1 cut(s) 169
HgaI GACGC 1 cut(s) 188
HhaI GCGC 2 cut(s) 149, 566
Hin1I GRCGYC 2 cut(s) 72, 147
Hin1II CATG 1 cut(s) 691
Hin6I GCGC 2 cut(s) 147, 564
HinP1I GCGC 2 cut(s) 147, 564
HindIII AAGCTT 2 cut(s) 366, 611
HinfI GANTC 4 cut(s) 95, 267, 380, 596
HpaII CCGG 1 cut(s) 169
HphI GGTGA 2 cut(s) 40, 253
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 5 cut(s) 61, 94, 304, 550, 609
Hpy188III TCNNGA 7 cut(s) 85, 169, 253, 264, 338, 377, 389
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 5 cut(s) 65, 71, 74, 119, 182
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 2 cut(s) 72, 159
HpyCH4V TGCA 3 cut(s) 432, 592, 687
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 133, 188
HpyF3I CTNAG 5 cut(s) 132, 198, 303, 626, 717
HpySE526I ACGT 2 cut(s) 72, 159
Hsp92I GRCGYC 2 cut(s) 72, 147
Hsp92II CATG 1 cut(s) 691
HspAI GCGC 2 cut(s) 147, 564
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 168
Kzo9I GATC 2 cut(s) 171, 340
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 303, 391
LweI GCATC 2 cut(s) 579, 689
MaeII ACGT 2 cut(s) 72, 159
MalI GATC 2 cut(s) 173, 342
MboI GATC 2 cut(s) 171, 340
MboII GAAGA 2 cut(s) 46, 100
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 4 cut(s) 372, 438, 616, 693
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 84
MroI TCCGGA 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 1 cut(s) 615
MslI CAYNNNNRTG 1 cut(s) 640
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 110, 133, 188
NarI GGCGCC 1 cut(s) 147
NdeII GATC 2 cut(s) 171, 340
NlaIII CATG 1 cut(s) 691
NlaIV GGNNCC 2 cut(s) 148, 173
NspV TTCGAA 1 cut(s) 9
OliI CACNNNNGTG 1 cut(s) 640
PceI AGGCCT 1 cut(s) 715
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 117
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 3 cut(s) 95, 267, 596
PflFI GACNNNGTC 1 cut(s) 71
PkrI GCNGC 3 cut(s) 23, 293, 406
PleI GAGTC 1 cut(s) 388
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 148, 173
PsrI GAACNNNNNNTAC 2 cut(s) 35, 67
PsuI RGATCY 1 cut(s) 171
PsyI GACNNNGTC 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 3 cut(s) 44, 463, 516
RsaNI GTAC 3 cut(s) 43, 462, 515
RseI CAYNNNNRTG 1 cut(s) 640
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 3 cut(s) 22, 292, 405
Sau3AI GATC 2 cut(s) 171, 340
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 333
SfaNI GCATC 2 cut(s) 579, 689
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 640
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 4 cut(s) 372, 438, 616, 693
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 3 cut(s) 21, 189, 350
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 683
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 2 cut(s) 75, 162
TaqI TCGA 3 cut(s) 9, 84, 207
TasI AATT 4 cut(s) 372, 438, 616, 693
TatI WGTACW 2 cut(s) 42, 461
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 3 cut(s) 95, 267, 596
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 1 cut(s) 515
TseI GCWGC 2 cut(s) 291, 404
TspDTI ATGAA 4 cut(s) 259, 536, 593, 610
TspGWI ACGGA 2 cut(s) 265, 560
TspRI CASTG 1 cut(s) 515
Tth111I GACNNNGTC 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 600
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.