Rh1DG463800

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003421
Physical Location & Seq
Reverse (-)
66611116 .. 66613231
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG463800.1

Sequence Viewer

Length: 975 bp
ATGTGGTTCGAGATCATCTGCGGCCTGGTGATTTACAGACTGTACAAGTTGATCTTCTCCGACGGCGACGACGTCTTGGATGTCGAGACCTCTGATTCTACCGCTCTCTTCGCCGTCGCCGACAGGCTCGCTAAGCTCTACGATGGCGCCAAGGTCTACGTTGGCCTCCGGATCCCCGACGCCGACACCGCTTCCCCTCAGCACATCGATTTGGTTCTCCTCAACAAAGGGGAGGCAGTGGTGATAGCCGTGAAGAACTTGTCAGGATTCATCTCCGTCAAGCCCGATGGCAGCTGGGTTTCTGAGAGCCTCACTAAACACAAAATAGAGCAGCATCCTGATCCACTGGCGGAGACAAAAAGACAAGCTTCAATTCTTGAGTCCTATCTTGAACAAAGAGGAGCTGCTTTACCAGAAGGATATTTGTCTTGCAAAGTAATACTTTCCAATCCAAAAGTATGTACCATTCAGTCAAGCAGTTTCCCACCAGAAATCATTACTTATGACCAGTGGGTACAAATGAAACCAGAACCAAAAAGTATGTTTTCTGGTTGGATGAAGGGCGCATTCCGTGGTGGAAAGAAAGAGATGCAAGAATCTATTCATCAGAAGCTTAATTTCATTCTTAGTACAGCACCAATGTGGGATAGGTTGGAGCTTAAAGGTAACAAGTATGTTCTTGGGGAATTTCTGGAATTCAAGGGTAAGCAGGAAGATGTTCAGGCTTTGAGGCATATTAAAAGATCAAAAATTAGCCGCCTTGTTGTCCAAAAGACAAGCATGCTTGGATTTGCCCCTTCAAGGCTCCAAGTTCTGTATACTCGTCGTGATTACCGAAGTGAAGGAGCTTCAGCTTCTGAGTGGAAAGAAGTAAATGTAAGGTCAAGCACGGAGGTTGTCTTTCAGCTTGACAATTCTTCAAAAGTCCGCAAGTTTAAGCTCTCCTCAATTTGTTCTATGTCTCTGAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

324

Amino Acids

36.5

Weight (kDa)

8.95

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 37 - 143 1.3e-12 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 104
AccI GTMKAC 2 cut(s) 156, 818
AccIII TCCGGA 1 cut(s) 168
AciI CCGC 6 cut(s) 21, 102, 189, 350, 757, 928
AclWI GGATC 3 cut(s) 166, 179, 335
AcsI RAATTY 2 cut(s) 686, 695
AcuI CTGAAG 1 cut(s) 834
AcyI GRCGYC 3 cut(s) 72, 147, 180
AfaI GTAC 4 cut(s) 44, 463, 516, 631
AfiI CCNNNNNNNGG 1 cut(s) 801
AgsI TTSAA 5 cut(s) 372, 392, 700, 801, 921
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 1 cut(s) 640
Alw26I GTCTC 3 cut(s) 80, 347, 966
AlwI GGATC 3 cut(s) 166, 179, 335
AlwNI CAGNNNCTG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 168
AoxI GGCC 2 cut(s) 22, 163
ApeKI GCWGC 3 cut(s) 291, 331, 404
ApoI RAATTY 2 cut(s) 686, 695
Asp700I GAANNNNTTC 2 cut(s) 600, 717
AspLEI GCGC 2 cut(s) 149, 566
AsuHPI GGTGA 2 cut(s) 40, 253
BamHI GGATCC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 146
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 303, 343, 391
BccI CCATC 2 cut(s) 137, 281
BceAI ACGGC 3 cut(s) 79, 98, 233
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 3 cut(s) 80, 347, 966
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 5 cut(s) 22, 292, 332, 405, 757
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 5 cut(s) 23, 293, 333, 406, 758
Bme1390I CCNGG 1 cut(s) 26
BmiI GGNNCC 3 cut(s) 148, 173, 806
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 343, 579
Bpu10I CCTNAGC 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 207
BsaHI GRCGYC 3 cut(s) 72, 147, 180
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 150, 571
BsaWI WCCGGW 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 2 cut(s) 351, 508
BseAI TCCGGA 1 cut(s) 168
BseBI CCWGG 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 150, 571
BseGI GGATG 3 cut(s) 85, 334, 561
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMII CTCAG 4 cut(s) 212, 294, 849, 956
BseNI ACTGG 2 cut(s) 351, 508
BseRI GAGGAG 3 cut(s) 209, 414, 934
BseXI GCAGC 3 cut(s) 303, 343, 391
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 2 cut(s) 24, 165
BshNI GGYRCC 1 cut(s) 146
BshVI ATCGAT 1 cut(s) 207
BsiSI CCGG 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 801
BsmAI GTCTC 3 cut(s) 80, 347, 966
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 2 cut(s) 24, 165
Bso31I GGTCTC 1 cut(s) 80
Bsp13I TCCGGA 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 5 cut(s) 12, 51, 171, 340, 743
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 6 cut(s) 21, 102, 189, 350, 757, 928
BspANI GGCC 2 cut(s) 24, 165
BspCNI CTCAG 4 cut(s) 211, 295, 850, 957
BspDI ATCGAT 1 cut(s) 207
BspEI TCCGGA 1 cut(s) 168
BspLI GGNNCC 3 cut(s) 148, 173, 806
BspPI GGATC 3 cut(s) 166, 179, 335
BspT107I GGYRCC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 2 cut(s) 351, 508
BssECI CCNNGG 2 cut(s) 150, 571
BssMI GATC 5 cut(s) 12, 51, 171, 340, 743
BssNAI GTATAC 1 cut(s) 819
BssNI GRCGYC 3 cut(s) 72, 147, 180
BssT1I CCWWGG 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 819
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 3 cut(s) 72, 147, 180
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 129, 782
BstDEI CTNAG 6 cut(s) 132, 198, 303, 626, 858, 965
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 3 cut(s) 85, 334, 561
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 566
BstKTI GATC 5 cut(s) 15, 54, 174, 343, 746
BstMAI GTCTC 3 cut(s) 80, 347, 966
BstMBI GATC 5 cut(s) 12, 51, 171, 340, 743
BstMWI GCNNNNNNNGC 3 cut(s) 110, 133, 188
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 303, 343, 391
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
BstZ17I GTATAC 1 cut(s) 819
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 2 cut(s) 24, 165
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 571
BtsCI GGATG 3 cut(s) 85, 334, 561
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 344, 515
Cac8I GCNNGC 2 cut(s) 129, 782
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 2 cut(s) 149, 566
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 188
Csp6I GTAC 4 cut(s) 43, 462, 515, 630
CviAII CATG 1 cut(s) 781
CviQI GTAC 4 cut(s) 43, 462, 515, 630
DdeI CTNAG 6 cut(s) 132, 198, 303, 626, 858, 965
DinI GGCGCC 1 cut(s) 148
DpnI GATC 5 cut(s) 14, 53, 173, 342, 745
DpnII GATC 5 cut(s) 12, 51, 171, 340, 743
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 834
EcoRI GAATTC 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 1 cut(s) 784
FaiI YATR 8 cut(s) 460, 504, 542, 675, 735, 782, 819, 959
FalI AAGNNNNNCTT 6 cut(s) 38, 70, 352, 384, 426, 458
FatI CATG 1 cut(s) 780
FblI GTMKAC 2 cut(s) 156, 818
Fnu4HI GCNGC 5 cut(s) 22, 292, 332, 405, 757
FokI GGATG 3 cut(s) 92, 321, 568
Fsp4HI GCNGC 5 cut(s) 22, 292, 332, 405, 757
GlaI GCGC 2 cut(s) 148, 565
GluI GCNGC 5 cut(s) 22, 292, 332, 405, 757
GsaI CCCAGC 1 cut(s) 298
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 24, 165
HapII CCGG 1 cut(s) 169
HgaI GACGC 1 cut(s) 188
HhaI GCGC 2 cut(s) 149, 566
Hin1I GRCGYC 3 cut(s) 72, 147, 180
Hin1II CATG 1 cut(s) 784
Hin6I GCGC 2 cut(s) 147, 564
HinP1I GCGC 2 cut(s) 147, 564
HindIII AAGCTT 2 cut(s) 366, 611
HinfI GANTC 4 cut(s) 95, 267, 380, 596
HpaII CCGG 1 cut(s) 169
HphI GGTGA 2 cut(s) 40, 253
Hpy166II GTNNAC 2 cut(s) 157, 819
Hpy188I TCNGA 6 cut(s) 61, 94, 304, 609, 859, 966
Hpy188III TCNNGA 9 cut(s) 10, 85, 169, 264, 338, 377, 389, 692, 827
Hpy8I GTNNAC 2 cut(s) 157, 819
Hpy99I CGWCG 6 cut(s) 65, 71, 74, 119, 182, 828
HpyAV CCTTC 4 cut(s) 410, 553, 807, 836
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 2 cut(s) 72, 159
HpyCH4V TGCA 2 cut(s) 432, 592
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 133, 188
HpyF3I CTNAG 6 cut(s) 132, 198, 303, 626, 858, 965
HpySE526I ACGT 2 cut(s) 72, 159
Hsp92I GRCGYC 3 cut(s) 72, 147, 180
Hsp92II CATG 1 cut(s) 784
HspAI GCGC 2 cut(s) 147, 564
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 168
Kzo9I GATC 5 cut(s) 12, 51, 171, 340, 743
LmnI GCTCC 4 cut(s) 401, 655, 810, 845
Lsp1109I GCAGC 3 cut(s) 303, 343, 391
LweI GCATC 2 cut(s) 343, 579
MaeII ACGT 2 cut(s) 72, 159
MaeIII GTNAC 1 cut(s) 665
MalI GATC 5 cut(s) 14, 53, 173, 342, 745
MbiI CCGCTC 1 cut(s) 104
MboI GATC 5 cut(s) 12, 51, 171, 340, 743
MboII GAAGA 5 cut(s) 46, 100, 265, 725, 909
MflI RGATCY 1 cut(s) 171
MluCI AATT 7 cut(s) 372, 616, 686, 695, 750, 913, 948
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 3 cut(s) 84, 533, 633
MroI TCCGGA 1 cut(s) 168
MroXI GAANNNNTTC 2 cut(s) 600, 717
MseI TTAA 5 cut(s) 615, 660, 738, 936, 973
MslI CAYNNNNRTG 1 cut(s) 640
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 110, 133, 188
NarI GGCGCC 1 cut(s) 147
NdeII GATC 5 cut(s) 12, 51, 171, 340, 743
NlaIII CATG 1 cut(s) 784
NlaIV GGNNCC 3 cut(s) 148, 173, 806
NspI RCATGY 1 cut(s) 784
OliI CACNNNNGTG 1 cut(s) 640
PaeI GCATGC 1 cut(s) 784
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 117, 832
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 2 cut(s) 600, 717
PfeI GAWTC 3 cut(s) 95, 267, 596
PflFI GACNNNGTC 1 cut(s) 71
PkrI GCNGC 5 cut(s) 23, 293, 333, 406, 758
PleI GAGTC 1 cut(s) 388
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 148, 173, 806
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 171
PsyI GACNNNGTC 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 4 cut(s) 44, 463, 516, 631
RsaNI GTAC 4 cut(s) 43, 462, 515, 630
RseI CAYNNNNRTG 1 cut(s) 640
SaqAI TTAA 5 cut(s) 615, 660, 738, 936, 973
SatI GCNGC 5 cut(s) 22, 292, 332, 405, 757
Sau3AI GATC 5 cut(s) 12, 51, 171, 340, 743
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 26
SfaNI GCATC 2 cut(s) 343, 579
SfoI GGCGCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 640
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 1 cut(s) 784
Sse9I AATT 7 cut(s) 372, 616, 686, 695, 750, 913, 948
SsiI CCGC 6 cut(s) 21, 102, 189, 350, 757, 928
SspDI GGCGCC 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 2 cut(s) 75, 162
TaqI TCGA 3 cut(s) 9, 84, 207
TasI AATT 7 cut(s) 372, 616, 686, 695, 750, 913, 948
TatI WGTACW 2 cut(s) 42, 629
TauI GCSGC 2 cut(s) 24, 759
TfiI GAWTC 3 cut(s) 95, 267, 596
Tru1I TTAA 5 cut(s) 615, 660, 738, 936, 973
Tru9I TTAA 5 cut(s) 615, 660, 738, 936, 973
TscAI CASTG 3 cut(s) 243, 351, 515
TseI GCWGC 3 cut(s) 291, 331, 404
TspDTI ATGAA 5 cut(s) 259, 536, 572, 593, 610
TspGWI ACGGA 3 cut(s) 265, 560, 905
TspRI CASTG 3 cut(s) 243, 351, 515
Tth111I GACNNNGTC 1 cut(s) 71
XapI RAATTY 2 cut(s) 686, 695
XceI RCATGY 1 cut(s) 784
XmiI GTMKAC 2 cut(s) 156, 818
XmnI GAANNNNTTC 2 cut(s) 600, 717
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.