Rh1CG175100

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003421
Physical Location & Seq
Forward (+)
38583054 .. 38592722
9669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG175100.1

Sequence Viewer

Length: 534 bp
ATGAGACTGTACAAGTTGATCTTCTCCGACGGCGATGACGTCTTGGGTGTCGAGACCTCTGATTCTACTACTCTCTTCGCCATCGCTGACAGGCTTGCTAAGCTCTATGATGGCGACAAGGTCTCAGTTGGCCTCCGGATCCCCAACGCCGATACCGCTTCCCGTAAGCAAATCGATTTGGTTCTCAAAGGGGAGGCAGTGGTGATATCCATCAAGAACTTGTCAGGATTCATCTCCGTCAAGCCCGATAGCAGCTGGGTTTCTGAAAGCATCCCTAAGCACAAAGCATACCAGCATCCTAATACTATACCTCCCTCTATCTCCTCTGCAACCTTCCCCTTCTCCTTCAAGTCTGAGACTTGTCTAATCTCAACAACATGTCATCCTCGTCAATTCCTGCCAGCAACAAACCACTACCTTGATTCCTTTCTTACCATACTCTGCACCTCCTCCTCGGATTGCAGATACGTTGAGTCAGATCAGATACCCCGAGTCAGACTGAGTGGAACCGCCAGCCGCCAAGCTCTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

177

Amino Acids

19.5

Weight (kDa)

8.35

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AccIII TCCGGA 1 cut(s) 135
AciI CCGC 3 cut(s) 156, 510, 517
AclWI GGATC 2 cut(s) 133, 146
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 11
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 1 cut(s) 349
AluBI AGCT 3 cut(s) 103, 255, 524
AluI AGCT 3 cut(s) 103, 255, 524
Alw26I GTCTC 3 cut(s) 47, 127, 350
AlwI GGATC 2 cut(s) 133, 146
Ama87I CYCGRG 1 cut(s) 489
Aor13HI TCCGGA 1 cut(s) 135
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 489
BamHI GGATCC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 264
BccI CCATC 3 cut(s) 89, 104, 218
BceAI ACGGC 1 cut(s) 46
BcoDI GTCTC 3 cut(s) 47, 127, 350
BisI GCNGC 2 cut(s) 253, 517
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 2 cut(s) 254, 518
BmeT110I CYCGRG 1 cut(s) 489
BmiI GGNNCC 2 cut(s) 140, 508
BmsI GCATC 2 cut(s) 279, 304
Bpu10I CCTNAGC 1 cut(s) 276
Bpu1102I GCTNAGC 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 2 cut(s) 47, 127
BsaJI CCNNGG 1 cut(s) 453
BsaWI WCCGGW 2 cut(s) 135, 528
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bse8I GATNNNNATC 1 cut(s) 209
BseAI TCCGGA 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 453
BseGI GGATG 3 cut(s) 270, 295, 382
BseJI GATNNNNATC 1 cut(s) 209
BseMII CTCAG 3 cut(s) 138, 345, 491
BseRI GAGGAG 3 cut(s) 313, 439, 442
BseXI GCAGC 1 cut(s) 264
BseYI CCCAGC 1 cut(s) 255
BsgI GTGCAG 1 cut(s) 427
BshFI GGCC 1 cut(s) 132
BshVI ATCGAT 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 2 cut(s) 136, 529
BsmAI GTCTC 3 cut(s) 47, 127, 350
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 2 cut(s) 47, 127
BsoBI CYCGRG 1 cut(s) 489
Bsp13I TCCGGA 1 cut(s) 135
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 3 cut(s) 18, 138, 478
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 3 cut(s) 156, 510, 517
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 3 cut(s) 137, 346, 492
BspDI ATCGAT 1 cut(s) 174
BspEI TCCGGA 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 140, 508
BspPI GGATC 2 cut(s) 133, 146
BspTNI GGTCTC 2 cut(s) 47, 127
BsrGI TGTACA 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 3 cut(s) 18, 138, 478
BssNI GRCGYC 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 9
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 39
BstAUI TGTACA 1 cut(s) 9
BstC8I GCNNGC 3 cut(s) 96, 402, 514
BstDEI CTNAG 5 cut(s) 99, 124, 276, 354, 500
BstF5I GGATG 3 cut(s) 270, 295, 382
BstKTI GATC 3 cut(s) 21, 141, 481
BstMAI GTCTC 3 cut(s) 47, 127, 350
BstMBI GATC 3 cut(s) 18, 138, 478
BstMWI GCNNNNNNNGC 2 cut(s) 100, 155
BstNSI RCATGY 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 132
BsuTUI ATCGAT 1 cut(s) 174
BtgZI GCGATG 2 cut(s) 48, 67
BtsCI GGATG 3 cut(s) 270, 295, 382
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 96, 402, 514
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 1 cut(s) 10
CviAII CATG 1 cut(s) 378
CviJI RGCY 7 cut(s) 94, 103, 132, 244, 255, 516, 524
CviKI_1 RGCY 7 cut(s) 94, 103, 132, 244, 255, 516, 524
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 5 cut(s) 99, 124, 276, 354, 500
DpnI GATC 3 cut(s) 20, 140, 480
DpnII GATC 3 cut(s) 18, 138, 478
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco31I GGTCTC 2 cut(s) 47, 127
Eco32I GATATC 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 489
EcoRV GATATC 1 cut(s) 207
FaeI CATG 1 cut(s) 381
FaiI YATR 5 cut(s) 108, 289, 308, 379, 437
FalI AAGNNNNNCTT 1 cut(s) 37
FatI CATG 1 cut(s) 377
Fnu4HI GCNGC 2 cut(s) 253, 517
FokI GGATG 3 cut(s) 257, 282, 369
Fsp4HI GCNGC 2 cut(s) 253, 517
GluI GCNGC 2 cut(s) 253, 517
GsaI CCCAGC 1 cut(s) 259
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 136, 529
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 1 cut(s) 381
HinfI GANTC 5 cut(s) 62, 228, 422, 473, 492
HpaII CCGG 2 cut(s) 136, 529
HphI GGTGA 1 cut(s) 214
Hpy188I TCNGA 8 cut(s) 28, 61, 265, 355, 457, 478, 483, 497
Hpy188III TCNNGA 4 cut(s) 52, 136, 214, 225
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 343, 349, 355
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 2 cut(s) 39, 468
HpyCH4V TGCA 3 cut(s) 329, 444, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 155
HpyF3I CTNAG 5 cut(s) 99, 124, 276, 354, 500
HpySE526I ACGT 2 cut(s) 39, 468
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 1 cut(s) 381
Kpn2I TCCGGA 1 cut(s) 135
Kzo9I GATC 3 cut(s) 18, 138, 478
LpnPI CCDG 8 cut(s) 76, 149, 210, 241, 305, 410, 414, 526
Lsp1109I GCAGC 1 cut(s) 264
LweI GCATC 2 cut(s) 279, 304
MaeII ACGT 2 cut(s) 39, 468
MalI GATC 3 cut(s) 20, 140, 480
MboI GATC 3 cut(s) 18, 138, 478
MboII GAAGA 2 cut(s) 13, 67
MflI RGATCY 1 cut(s) 138
MluCI AATT 1 cut(s) 392
MlyI GAGTC 2 cut(s) 482, 501
MmeI TCCRAC 1 cut(s) 51
MroI TCCGGA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 2 cut(s) 136, 529
MwoI GCNNNNNNNGC 2 cut(s) 100, 155
NdeII GATC 3 cut(s) 18, 138, 478
NlaIII CATG 1 cut(s) 381
NlaIV GGNNCC 2 cut(s) 140, 508
NspI RCATGY 1 cut(s) 381
PciI ACATGT 1 cut(s) 377
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PfeI GAWTC 3 cut(s) 62, 228, 422
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 2 cut(s) 254, 518
PleI GAGTC 2 cut(s) 481, 500
PpsI GAGTC 2 cut(s) 481, 500
PscI ACATGT 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 255
PspN4I GGNNCC 2 cut(s) 140, 508
PsuI RGATCY 1 cut(s) 138
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SatI GCNGC 2 cut(s) 253, 517
Sau3AI GATC 3 cut(s) 18, 138, 478
SchI GAGTC 2 cut(s) 482, 501
SfaNI GCATC 2 cut(s) 279, 304
Sse9I AATT 1 cut(s) 392
SsiI CCGC 3 cut(s) 156, 510, 517
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 2 cut(s) 42, 471
TaqI TCGA 2 cut(s) 51, 174
TasI AATT 1 cut(s) 392
TatI WGTACW 1 cut(s) 9
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 3 cut(s) 62, 228, 422
TscAI CASTG 1 cut(s) 204
TseI GCWGC 1 cut(s) 252
TspDTI ATGAA 1 cut(s) 220
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 204
Tth111I GACNNNGTC 1 cut(s) 119
XceI RCATGY 1 cut(s) 381
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.