FvH4_7g34140

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003421
Physical Location & Seq
Reverse (-)
24109451 .. 24111986
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g34140.t1

Sequence Viewer

Length: 975 bp
ATGTGGTTCGAGATCATCTGCGGGCTGGTGATTTACAGTCTGTACAAGTTGTTTTTCTCCGACGGCGACGACCTCTTGGATGTGGAGACCTCTGATTCTACTGCTCTGTTTGCAGTCGCCGACAGGCTCTCCAAGCTCTACGATGGCGCCAAGGTCTACGTCGGCCTCCGCATTCCCGACGCTGATACCTCTTCCCCTCAGCCCATCGATTTGGTTCTCCTCAACAAAGGGGAGGCAGTGGTGATCACCGTCAAGAACTTGAAAGGATTCATCTCCGTCAAGCCCGATGGCAGCTGGGTTTCTGAGAGCCTCTCTAAACACAAAGCAGACCACCATCCTGATCCTCTGGCTGAGACTAAAAGACAAGCTTCAATTCTTGAATCCTATCTTGAACAAAGAGGACTTGATTTACCACAAGGATATTTGTCCTGCAAAGTAATACTTTCCAATCCTAAAGTTTGTACTATTCAGTCGAGCAGTTTCCCATCAGAAATCATTACTTATGACCAGTGGGTACAAATGAAACCTGAACCAAAAAGTATGCTTTCTGGTTGGATGAAGGGCGCATTCCGTGGTGGAAAGAAAGAGATGCAAGAATCTATTCATCAGAAGCTTGATTTTATTCTGAGTACAGCACCAATGTGGGATAGGTTGGAGCTTAAAGGTAACAAGTATGTTCTTGGAGAATTTCTGGAATTCAAGGGTAAGCAGGAAGATGTTCAGGCTTTGAGACATATCAAAAGATCAAAAATTAGCCGCCTTGTTGTCCAAAAGACAAGCATGCTTGGATTTGCCCCTTCAAGGCTCCAAGTTCTGTACGCTCGCCGTGATTACCGCAGTGAAGGAACTTCAACTTCTGACTGGAAAGAAGCAAGTGTAAGGTCGAGCACGGAGGTTGTCTTTCAGCTTAACAATTCTCCAAAAGTCCGCAAGTTTAAGCTCTCATCAATTAGTTCTATGTCTCTGAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

325

Amino Acids

36.47

Weight (kDa)

9.0

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 37 - 137 2.2e-11 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 156
AciI CCGC 5 cut(s) 21, 169, 757, 835, 928
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 2 cut(s) 686, 695
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 5 cut(s) 44, 463, 516, 631, 818
AfiI CCNNNNNNNGG 1 cut(s) 801
AgsI TTSAA 7 cut(s) 262, 372, 380, 392, 700, 801, 852
AleI CACNNNNGTG 1 cut(s) 640
AluBI AGCT 7 cut(s) 136, 294, 368, 613, 658, 907, 940
AluI AGCT 7 cut(s) 136, 294, 368, 613, 658, 907, 940
Alw21I GWGCWC 1 cut(s) 890
Alw26I GTCTC 4 cut(s) 80, 347, 724, 966
AlwI GGATC 1 cut(s) 335
AoxI GGCC 1 cut(s) 163
ApeKI GCWGC 1 cut(s) 291
ApoI RAATTY 2 cut(s) 686, 695
Asp700I GAANNNNTTC 3 cut(s) 266, 600, 717
AspLEI GCGC 2 cut(s) 149, 566
AsuHPI GGTGA 3 cut(s) 40, 238, 253
BanI GGYRCC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 890
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 303
BccI CCATC 5 cut(s) 137, 212, 281, 342, 493
BceAI ACGGC 2 cut(s) 79, 810
BclI TGATCA 1 cut(s) 243
BcoDI GTCTC 4 cut(s) 80, 347, 724, 966
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 2 cut(s) 292, 757
BlsI GCNGC 2 cut(s) 293, 758
BmiI GGNNCC 2 cut(s) 148, 806
BmsI GCATC 1 cut(s) 579
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu10I CCTNAGC 1 cut(s) 198
Bsa29I ATCGAT 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 150, 571
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 2 cut(s) 508, 866
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 150, 571
BseGI GGATG 3 cut(s) 85, 334, 561
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMII CTCAG 5 cut(s) 212, 294, 342, 617, 956
BseNI ACTGG 2 cut(s) 508, 866
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 303
BseYI CCCAGC 1 cut(s) 294
BshFI GGCC 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 146
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 890
BslI CCNNNNNNNGG 1 cut(s) 801
BsmAI GTCTC 4 cut(s) 80, 347, 724, 966
BsmI GAATGC 2 cut(s) 171, 566
BsnI GGCC 1 cut(s) 165
Bso31I GGTCTC 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 890
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 4 cut(s) 12, 243, 340, 743
BspACI CCGC 5 cut(s) 21, 169, 757, 835, 928
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 5 cut(s) 211, 295, 343, 618, 957
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 2 cut(s) 148, 806
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 2 cut(s) 508, 866
BssECI CCNNGG 2 cut(s) 150, 571
BssMI GATC 4 cut(s) 12, 243, 340, 743
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 38, 250
Bst6I CTCTTC 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 3 cut(s) 23, 782, 823
BstDEI CTNAG 5 cut(s) 198, 303, 351, 626, 965
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 3 cut(s) 85, 334, 561
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 566
BstKTI GATC 4 cut(s) 15, 246, 343, 746
BstMAI GTCTC 4 cut(s) 80, 347, 724, 966
BstMBI GATC 4 cut(s) 12, 243, 340, 743
BstMWI GCNNNNNNNGC 2 cut(s) 110, 133
BstNSI RCATGY 1 cut(s) 784
BstV1I GCAGC 1 cut(s) 303
BstXI CCANNNNNNTGG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 1 cut(s) 165
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 571
BtsCI GGATG 3 cut(s) 85, 334, 561
BtsI GCAGTG 2 cut(s) 243, 844
BtsIMutI CAGTG 3 cut(s) 243, 515, 844
Cac8I GCNNGC 3 cut(s) 23, 782, 823
CfoI GCGC 2 cut(s) 149, 566
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 188
Csp6I GTAC 5 cut(s) 43, 462, 515, 630, 817
CviAII CATG 1 cut(s) 781
CviQI GTAC 5 cut(s) 43, 462, 515, 630, 817
DdeI CTNAG 5 cut(s) 198, 303, 351, 626, 965
DinI GGCGCC 1 cut(s) 148
DpnI GATC 4 cut(s) 14, 245, 342, 745
DpnII GATC 4 cut(s) 12, 243, 340, 743
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 695
EcoT14I CCWWGG 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 1 cut(s) 784
FaiI YATR 6 cut(s) 504, 542, 675, 735, 782, 959
FalI AAGNNNNNCTT 4 cut(s) 352, 384, 426, 458
FatI CATG 1 cut(s) 780
FauI CCCGC 1 cut(s) 14
FbaI TGATCA 1 cut(s) 243
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 292, 757
FokI GGATG 3 cut(s) 92, 321, 568
Fsp4HI GCNGC 2 cut(s) 292, 757
GlaI GCGC 2 cut(s) 148, 565
GluI GCNGC 2 cut(s) 292, 757
GsaI CCCAGC 1 cut(s) 298
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 1 cut(s) 165
HgaI GACGC 1 cut(s) 188
HhaI GCGC 2 cut(s) 149, 566
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 1 cut(s) 784
Hin6I GCGC 2 cut(s) 147, 564
HinP1I GCGC 2 cut(s) 147, 564
HindIII AAGCTT 2 cut(s) 366, 611
HinfI GANTC 4 cut(s) 95, 267, 380, 596
HphI GGTGA 3 cut(s) 40, 238, 253
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 8 cut(s) 61, 94, 304, 490, 609, 627, 859, 966
Hpy188III TCNNGA 7 cut(s) 10, 176, 253, 338, 377, 389, 692
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 4 cut(s) 65, 71, 164, 182
HpyAV CCTTC 3 cut(s) 553, 807, 836
HpyCH4III ACNGT 2 cut(s) 38, 250
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 3 cut(s) 113, 432, 592
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 133
HpyF3I CTNAG 5 cut(s) 198, 303, 351, 626, 965
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 1 cut(s) 784
HspAI GCGC 2 cut(s) 147, 564
KasI GGCGCC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 4 cut(s) 12, 243, 340, 743
LmnI GCTCC 2 cut(s) 655, 810
Lsp1109I GCAGC 1 cut(s) 303
LweI GCATC 1 cut(s) 579
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 665
MalI GATC 4 cut(s) 14, 245, 342, 745
MboI GATC 4 cut(s) 12, 243, 340, 743
MboII GAAGA 2 cut(s) 183, 725
MhlI GDGCHC 1 cut(s) 890
MluCI AATT 6 cut(s) 372, 686, 695, 750, 913, 948
Mly113I GGCGCC 1 cut(s) 147
MmeI TCCRAC 3 cut(s) 84, 533, 633
MroXI GAANNNNTTC 3 cut(s) 266, 600, 717
MseI TTAA 4 cut(s) 660, 909, 936, 973
MslI CAYNNNNRTG 1 cut(s) 640
MspA1I CMGCKG 1 cut(s) 294
Mva1269I GAATGC 2 cut(s) 171, 566
MwoI GCNNNNNNNGC 2 cut(s) 110, 133
NarI GGCGCC 1 cut(s) 147
NdeII GATC 4 cut(s) 12, 243, 340, 743
NlaIII CATG 1 cut(s) 784
NlaIV GGNNCC 2 cut(s) 148, 806
NspI RCATGY 1 cut(s) 784
OliI CACNNNNGTG 1 cut(s) 640
PaeI GCATGC 1 cut(s) 784
PctI GAATGC 2 cut(s) 171, 566
PdmI GAANNNNTTC 3 cut(s) 266, 600, 717
PfeI GAWTC 4 cut(s) 95, 267, 380, 596
PkrI GCNGC 2 cut(s) 293, 758
PluTI GGCGCC 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 148, 806
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 5 cut(s) 44, 463, 516, 631, 818
RsaNI GTAC 5 cut(s) 43, 462, 515, 630, 817
RseI CAYNNNNRTG 1 cut(s) 640
SaqAI TTAA 4 cut(s) 660, 909, 936, 973
SatI GCNGC 2 cut(s) 292, 757
Sau3AI GATC 4 cut(s) 12, 243, 340, 743
SduI GDGCHC 1 cut(s) 890
SfaNI GCATC 1 cut(s) 579
SfoI GGCGCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 640
SphI GCATGC 1 cut(s) 784
Sse9I AATT 6 cut(s) 372, 686, 695, 750, 913, 948
SsiI CCGC 5 cut(s) 21, 169, 757, 835, 928
SspDI GGCGCC 1 cut(s) 146
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 38, 250
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 9, 207, 473, 884
TasI AATT 6 cut(s) 372, 686, 695, 750, 913, 948
TatI WGTACW 3 cut(s) 42, 461, 629
TauI GCSGC 1 cut(s) 759
TfiI GAWTC 4 cut(s) 95, 267, 380, 596
Tru1I TTAA 4 cut(s) 660, 909, 936, 973
Tru9I TTAA 4 cut(s) 660, 909, 936, 973
TscAI CASTG 3 cut(s) 243, 515, 844
TseI GCWGC 1 cut(s) 291
TspDTI ATGAA 4 cut(s) 259, 536, 572, 593
TspGWI ACGGA 3 cut(s) 265, 560, 905
TspRI CASTG 3 cut(s) 243, 515, 844
XapI RAATTY 2 cut(s) 686, 695
XceI RCATGY 1 cut(s) 784
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 3 cut(s) 266, 600, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.