RchiOBHm_Chr7g0241921

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003421
Physical Location & Seq
Forward (+)
67891499 .. 67892639
1141 bp
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UTR
Exon/CDS
Intron
PRQ21681

Sequence Viewer

Length: 519 bp
ATGAGACTCCACAGTCCCAACAGCAGACTCAACACCTTCTACCATAAACCCACCAAAAACCATGGGATTGTGCAAGCCTCCTTCTACTCCACTATTCATCATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCCCCCCAATTTCATTTCGGATCAGTCACTGGGCAAATCGCAGTCTCCATCTGCCAGTCCAACCCCCAATTTTCTAGGTTCGAAATCATCTGCGGGTTGGTGATTTACAGACTCTACAAGTTGATCTTCTCCAACGGTGACGACGTCTTGGGTGTCGAGACCTCTGATTCTACTGCTCTATTCGCCATCGCTGACAGGCTCGCTAAGCTCTATGATGGCGACAAGGTCTCCGTTGGCCTCCGGATCCCCGACACCGATACCGCTTCCCCTAAGCAAATCGATTTGGTTCTCAAAGGGGAGGCAGTGGTGATAGCCGTCAAGAACTTGTCAGGATTCATCTCCGTTAAGCCCGGTGGCAGTTGGGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

172

Amino Acids

18.71

Weight (kDa)

8.93

Isoelectric Point (pI)

23.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 12
AatII GACGTC 1 cut(s) 294
AccIII TCCGGA 1 cut(s) 387
AciI CCGC 2 cut(s) 240, 408
AclWI GGATC 3 cut(s) 175, 385, 398
AcyI GRCGYC 1 cut(s) 291
AluBI AGCT 1 cut(s) 355
AluI AGCT 1 cut(s) 355
Alw26I GTCTC 3 cut(s) 196, 299, 379
AlwI GGATC 3 cut(s) 175, 385, 398
AlwNI CAGNNNCTG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 387
AoxI GGCC 1 cut(s) 382
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 3 cut(s) 259, 296, 466
AsuII TTCGAA 1 cut(s) 228
BamHI GGATCC 1 cut(s) 390
BccI CCATC 3 cut(s) 203, 341, 356
BceAI ACGGC 1 cut(s) 446
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 3 cut(s) 196, 299, 379
BfaI CTAG 1 cut(s) 222
BlpI GCTNAGC 1 cut(s) 351
Bme1390I CCNGG 1 cut(s) 498
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 498
BmrI ACTGGG 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 417
Bpu1102I GCTNAGC 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 2 cut(s) 299, 379
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 387
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bse1I ACTGG 2 cut(s) 181, 202
BseAI TCCGGA 1 cut(s) 387
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 61
BseNI ACTGG 2 cut(s) 181, 202
BshFI GGCC 1 cut(s) 384
BshVI ATCGAT 1 cut(s) 426
BsiSI CCGG 2 cut(s) 388, 498
BsmAI GTCTC 3 cut(s) 196, 299, 379
BsnI GGCC 1 cut(s) 384
Bso31I GGTCTC 2 cut(s) 299, 379
Bsp119I TTCGAA 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 387
Bsp143I GATC 3 cut(s) 167, 270, 390
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 2 cut(s) 240, 408
BspANI GGCC 1 cut(s) 384
BspDI ATCGAT 1 cut(s) 426
BspEI TCCGGA 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 392
BspPI GGATC 3 cut(s) 175, 385, 398
BspT104I TTCGAA 1 cut(s) 228
BspTNI GGTCTC 2 cut(s) 299, 379
BsrI ACTGG 2 cut(s) 181, 202
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 3 cut(s) 167, 270, 390
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 61
Bst4CI ACNGT 2 cut(s) 14, 284
BstACI GRCGYC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 75, 348
BstDEI CTNAG 2 cut(s) 351, 417
BstDSI CCRYGG 1 cut(s) 61
BstKTI GATC 3 cut(s) 170, 273, 393
BstMAI GTCTC 3 cut(s) 196, 299, 379
BstMBI GATC 3 cut(s) 167, 270, 390
BstMWI GCNNNNNNNGC 2 cut(s) 329, 352
BstSCI CCNGG 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 1 cut(s) 384
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 174, 456
Cac8I GCNNGC 2 cut(s) 75, 348
CaiI CAGNNNCTG 1 cut(s) 176
ClaI ATCGAT 1 cut(s) 426
CviAII CATG 1 cut(s) 62
CviJI RGCY 6 cut(s) 77, 346, 355, 384, 461, 496
CviKI_1 RGCY 6 cut(s) 77, 346, 355, 384, 461, 496
DdeI CTNAG 2 cut(s) 351, 417
DpnI GATC 3 cut(s) 169, 272, 392
DpnII GATC 3 cut(s) 167, 270, 390
DrdI GACNNNNNNGTC 1 cut(s) 12
DseDI GACNNNNNNGTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 61
Eco31I GGTCTC 2 cut(s) 299, 379
EcoT14I CCWWGG 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 61
FaeI CATG 1 cut(s) 65
FaiI YATR 3 cut(s) 45, 63, 360
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 1 cut(s) 61
FauI CCCGC 1 cut(s) 233
FspBI CTAG 1 cut(s) 222
HaeIII GGCC 1 cut(s) 384
HapII CCGG 2 cut(s) 388, 498
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 1 cut(s) 65
HinfI GANTC 5 cut(s) 6, 27, 258, 314, 480
HpaII CCGG 2 cut(s) 388, 498
HphI GGTGA 3 cut(s) 259, 296, 466
Hpy188I TCNGA 2 cut(s) 167, 313
Hpy188III TCNNGA 4 cut(s) 304, 388, 466, 477
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 46, 91
HpyCH4III ACNGT 2 cut(s) 14, 284
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 352
HpyF3I CTNAG 2 cut(s) 351, 417
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 387
Kzo9I GATC 3 cut(s) 167, 270, 390
LpnPI CCDG 6 cut(s) 162, 215, 328, 401, 462, 511
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 172, 284
MalI GATC 3 cut(s) 169, 272, 392
MboI GATC 3 cut(s) 167, 270, 390
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 390
MluCI AATT 2 cut(s) 155, 215
MlyI GAGTC 2 cut(s) 21, 252
MmeI TCCRAC 2 cut(s) 231, 303
MnlI CCTC 4 cut(s) 88, 319, 395, 439
MroI TCCGGA 1 cut(s) 387
MseI TTAA 1 cut(s) 492
MspI CCGG 2 cut(s) 388, 498
MspR9I CCNGG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 329, 352
NciI CCSGG 1 cut(s) 498
NcoI CCATGG 1 cut(s) 61
NdeII GATC 3 cut(s) 167, 270, 390
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 2 cut(s) 172, 284
NspV TTCGAA 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 2 cut(s) 314, 480
PflFI GACNNNGTC 2 cut(s) 290, 371
PleI GAGTC 2 cut(s) 21, 252
PpsI GAGTC 2 cut(s) 21, 252
PspN4I GGNNCC 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 390
PsyI GACNNNGTC 2 cut(s) 290, 371
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 3 cut(s) 167, 270, 390
SchI GAGTC 2 cut(s) 21, 252
ScrFI CCNGG 1 cut(s) 498
SetI ASST 6 cut(s) 38, 227, 294, 311, 357, 375
SfuI TTCGAA 1 cut(s) 228
Sse9I AATT 2 cut(s) 155, 215
SsiI CCGC 2 cut(s) 240, 408
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 2 cut(s) 14, 284
TaiI ACGT 1 cut(s) 294
TaqI TCGA 3 cut(s) 228, 303, 426
TasI AATT 2 cut(s) 155, 215
TfiI GAWTC 2 cut(s) 314, 480
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 2 cut(s) 181, 456
TseFI GTSAC 2 cut(s) 172, 284
Tsp45I GTSAC 2 cut(s) 172, 284
TspDTI ATGAA 3 cut(s) 86, 149, 472
TspGWI ACGGA 2 cut(s) 367, 478
TspRI CASTG 2 cut(s) 181, 456
Tth111I GACNNNGTC 2 cut(s) 290, 371
XspI CTAG 1 cut(s) 222
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.