Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001207

Physical Location & Seq

Genomic Coordinates Reverse (-)

24655115 .. 24657703

Gene Structure 2589 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G66200.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGAGTCTTCTTGCAGATCTTGTTAACCTTGACATCTCAGACAACAGTGAAAAGATCATCGCTGAATACATATGGGTTGGTGGTTCTGGTATGGACATGAGAAGCAAAGCCAGGACTCTCCCTGGACCTGTGACCGATCCATCAAAACTTCCAAAGTGGAACTATGATGGTTCAAGCACTGGTCAAGCTCCTGGTCAAGACAGTGAAGTGATCTTATACCCTCAAGCAATTTTCAAAGATCCATTCCGTAGAGGCAACAACATCCTTGTTATGTGTGATGCTTACACTCCAGCGGGAGAGCCAATCCCTACTAACAAGCGACATGCTGCGGCTGAGATCTTTGCTAACCCTGATGTTATTGCTGAAGTGCCATGGTATGGAATCGAACAAGAATACACTTTGTTGCAGAAGGATGTGAACTGGCCTCTTGGATGGCCCATTGGTGGCTTCCCTGGCCCTCAGGGACCATACTACTGCAGTATTGGAGCTGACAAATCTTTTGGAAGAGACATTGTTGATGCTCACTACAAAGCCTCTTTGTATGCTGGAATCAACATCAGTGGGATCAATGGAGAAGTCATGCCGGGACAATGGGAGTTCCAAGTCGGCCCATCGGTCGGTATCTCAGCTGCTGATGAAATATGGATCGCTCGTTACATTTTGGAGAGGATCACAGAGATTGCTGGTGTGGTTGTATCTTTTGACCCAAAACCTATTCCTGGTGACTGGAATGGAGCTGGTGCTCACACCAATTACAGTACTAAATCAATGAGGGAAGAAGGAGGATACGAGATAATCAAGAAGGCGATCGAGAAGCTTGGCTTGAGACACAAGGAACACATTTCCGCTTACGGTGAAGGAAACGAGCGTCGTCTCACGGGACACCATGAAACTGCTGACATCAACACTTTCCTTTGGGGTGTTGCGAACCGTGGTGCATCGATCCGAGTAGGACGTGACACCGAGAAAGAAGGGAAGGGATACTTTGAGGATAGGAGGCCAGCTTCAAACATGGACCCTTACGTTGTTACTTCCATGATTGCAGAGACTACACTCCTCTGGAACCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004356 GO:0005488 GO:0005507 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0005911 GO:0006082 GO:0006807 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010150 GO:0016020 GO:0016211 GO:0016874 GO:0016879 GO:0016880 GO:0022626 GO:0030054 GO:0030312 GO:0032501 GO:0032502 GO:0032991 GO:0042126 GO:0042128 GO:0042802 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0046872 GO:0046914 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071704 GO:0071941 GO:0071944 GO:0090693 GO:0099402 GO:1990904 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

356

Amino Acids

39.21

Weight (kDa)

5.14

Isoelectric Point (pI)

39.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_C	PF00120	176 - 356	7.1e-14	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48470 AT1G66200 AT1G66200 AT1G66200 AT3G17820 AT5G16570 AT5G16570 AT5G37600
fragaria_vesca	FvH4_1g30220 FvH4_3g32400 FvH4_4g36850 FvH4_5g17650
malus_domestica	MD09G1270600.v1.1 MD13G1006600.v1.1 MD14G1238700.v1.1 MD16G1001400.v1.1 MD17G1268700.v1.1
prunus_persica	Prupe.1G346600_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.5G236300_v2.0.a1
pyrus_communis	pycom09g18170 pycom14g19980 pycom17g26730
rosa_chinensis	RchiOBHm_Chr3g0491191 RchiOBHm_Chr4g0446601 RchiOBHm_Chr7g0178021
rosa_laevigata	RLG00000005481 RLG00000005647 RLG00000022854
rosa_multiflora	Rmu_sc0014162.1_g000003 Rmu_sc0015729.1_g000003 Rmu_sc0019011.1_g000005 Rmu_ssc0000144.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275280 Rroxscaffold_5G00386990 Rroxscaffold_6G00393090
rosa_rugosa	Rorug03G0247600 Rorug04G0372700 Rorug06G0412400 Rorug06G0412400
rosa_samantha	Rh3BG333800 Rh3DG331200 Rh4AG434300 Rh4BG443500 Rh4CG460800 Rh4DG442300 Rh7AG011900 Rh7BG011300 Rh7CG011900 Rh7DG011900
rosa_wichuraiana	Rw3G026210 Rw3G026250 Rw4G037130 Rw7G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	377
AciI	CCGC	3 cut(s)	293, 329, 846
AclWI	GGATC	6 cut(s)	131, 233, 572, 653, 677, 937
AcuI	CTGAAG	1 cut(s)	384
AfaI	GTAC	1 cut(s)	760
AfiI	CCNNNNNNNGG	4 cut(s)	377, 443, 617, 719
AgsI	TTSAA	3 cut(s)	174, 235, 1008
AjiI	CACGTC	1 cut(s)	956
AjnI	CCWGG	5 cut(s)	110, 121, 190, 451, 718
AluBI	AGCT	6 cut(s)	188, 488, 629, 737, 817, 1004
AluI	AGCT	6 cut(s)	188, 488, 629, 737, 817, 1004
Alw21I	GWGCWC	1 cut(s)	745
Alw26I	GTCTC	4 cut(s)	501, 820, 878, 1040
AlwI	GGATC	6 cut(s)	131, 233, 572, 653, 677, 937
AlwNI	CAGNNNCTG	1 cut(s)	632
AoxI	GGCC	5 cut(s)	422, 434, 454, 607, 998
ApeKI	GCWGC	2 cut(s)	326, 629
ArsI	GACNNNNNNTTYG	2 cut(s)	482, 514
AspS9I	GGNCC	6 cut(s)	125, 435, 455, 464, 608, 1015
AsuC2I	CCSGG	1 cut(s)	585
AsuHPI	GGTGA	2 cut(s)	734, 866
AvaII	GGWCC	3 cut(s)	125, 464, 1015
AxyI	CCTNAGG	1 cut(s)	459
Bbv12I	GWGCWC	1 cut(s)	745
BbvI	GCAGC	2 cut(s)	313, 616
BccI	CCATC	4 cut(s)	148, 161, 426, 619
BciT130I	CCWGG	5 cut(s)	112, 123, 192, 453, 720
BciVI	GTATCC	2 cut(s)	779, 974
BcnI	CCSGG	1 cut(s)	585
BcoDI	GTCTC	4 cut(s)	501, 820, 878, 1040
BfmI	CTRYAG	1 cut(s)	475
BfuI	GTATCC	2 cut(s)	779, 974
BglII	AGATCT	2 cut(s)	16, 336
BisI	GCNGC	3 cut(s)	327, 330, 630
BlsI	GCNGC	3 cut(s)	328, 331, 631
BmcAI	AGTACT	1 cut(s)	760
Bme1390I	CCNGG	6 cut(s)	112, 123, 192, 453, 585, 720
Bme18I	GGWCC	3 cut(s)	125, 464, 1015
BmgBI	CACGTC	1 cut(s)	956
BmgT120I	GGNCC	6 cut(s)	125, 435, 455, 464, 608, 1015
BmiI	GGNNCC	3 cut(s)	465, 1017, 1064
BmrFI	CCNGG	6 cut(s)	112, 123, 192, 453, 585, 720
BmsI	GCATC	3 cut(s)	268, 508, 947
BpmI	CTGGAG	1 cut(s)	273
BpuEI	CTTGAG	2 cut(s)	207, 844
BpuMI	CCSGG	1 cut(s)	585
Bsa29I	ATCGAT	1 cut(s)	941
BsaJI	CCNNGG	4 cut(s)	121, 371, 451, 931
Bsc4I	CCNNNNNNNGG	4 cut(s)	377, 443, 617, 719
Bse1I	ACTGG	3 cut(s)	184, 425, 731
Bse21I	CCTNAGG	1 cut(s)	459
BseBI	CCWGG	5 cut(s)	112, 123, 192, 453, 720
BseCI	ATCGAT	1 cut(s)	941
BseDI	CCNNGG	4 cut(s)	121, 371, 451, 931
BseGI	GGATG	3 cut(s)	261, 418, 437
BseLI	CCNNNNNNNGG	4 cut(s)	377, 443, 617, 719
BseMII	CTCAG	4 cut(s)	51, 324, 473, 639
BseNI	ACTGG	3 cut(s)	184, 425, 731
BseRI	GAGGAG	1 cut(s)	1046
BseXI	GCAGC	2 cut(s)	313, 616
Bsh1285I	CGRYCG	2 cut(s)	618, 810
BshFI	GGCC	5 cut(s)	424, 436, 456, 609, 1000
BshVI	ATCGAT	1 cut(s)	941
BsiEI	CGRYCG	2 cut(s)	618, 810
BsiHKAI	GWGCWC	1 cut(s)	745
BsiSI	CCGG	1 cut(s)	584
BslFI	GGGAC	3 cut(s)	477, 600, 894
BslI	CCNNNNNNNGG	4 cut(s)	377, 443, 617, 719
BsmAI	GTCTC	4 cut(s)	501, 820, 878, 1040
BsmBI	CGTCTC	1 cut(s)	878
BsmFI	GGGAC	3 cut(s)	477, 600, 894
BsnI	GGCC	5 cut(s)	424, 436, 456, 609, 1000
Bsp1286I	GDGCHC	1 cut(s)	745
Bsp19I	CCATGG	1 cut(s)	371
BspACI	CCGC	3 cut(s)	293, 329, 846
BspANI	GGCC	5 cut(s)	424, 436, 456, 609, 1000
BspCNI	CTCAG	4 cut(s)	50, 325, 472, 638
BspDI	ATCGAT	1 cut(s)	941
BspLI	GGNNCC	3 cut(s)	465, 1017, 1064
BspMAI	CTGCAG	1 cut(s)	479
BspPI	GGATC	6 cut(s)	131, 233, 572, 653, 677, 937
BsrI	ACTGG	3 cut(s)	184, 425, 731
BssECI	CCNNGG	4 cut(s)	121, 371, 451, 931
BssT1I	CCWWGG	1 cut(s)	371
Bst2UI	CCWGG	5 cut(s)	112, 123, 192, 453, 720
Bst4CI	ACNGT	5 cut(s)	47, 203, 758, 854, 932
Bst6I	CTCTTC	1 cut(s)	499
BstC8I	GCNNGC	1 cut(s)	1002
BstDEI	CTNAG	4 cut(s)	37, 333, 459, 625
BstDSI	CCRYGG	2 cut(s)	371, 931
BstF5I	GGATG	3 cut(s)	261, 418, 437
BstMAI	GTCTC	4 cut(s)	501, 820, 878, 1040
BstMCI	CGRYCG	2 cut(s)	618, 810
BstMWI	GCNNNNNNNGC	1 cut(s)	453
BstNI	CCWGG	5 cut(s)	112, 123, 192, 453, 720
BstNSI	RCATGY	1 cut(s)	326
BstSCI	CCNGG	6 cut(s)	110, 121, 190, 451, 583, 718
BstSFI	CTRYAG	1 cut(s)	475
BstV1I	GCAGC	2 cut(s)	313, 616
BstX2I	RGATCY	3 cut(s)	16, 238, 336
BstYI	RGATCY	3 cut(s)	16, 238, 336
Bsu15I	ATCGAT	1 cut(s)	941
Bsu36I	CCTNAGG	1 cut(s)	459
BsuI	GTATCC	2 cut(s)	779, 974
BsuRI	GGCC	5 cut(s)	424, 436, 456, 609, 1000
BsuTUI	ATCGAT	1 cut(s)	941
BtgI	CCRYGG	2 cut(s)	371, 931
BtgZI	GCGATG	1 cut(s)	43
BtrI	CACGTC	1 cut(s)	956
BtsCI	GGATG	3 cut(s)	261, 418, 437
BtsIMutI	CAGTG	4 cut(s)	52, 177, 208, 565
Cac8I	GCNNGC	1 cut(s)	1002
CaiI	CAGNNNCTG	1 cut(s)	632
Cfr13I	GGNCC	6 cut(s)	125, 435, 455, 464, 608, 1015
ClaI	ATCGAT	1 cut(s)	941
CseI	GACGC	1 cut(s)	857
Csp6I	GTAC	1 cut(s)	759
CspCI	CAANNNNNGTGG	2 cut(s)	541, 576
CviAII	CATG	7 cut(s)	97, 323, 372, 580, 887, 1012, 1036
CviQI	GTAC	1 cut(s)	759
DdeI	CTNAG	4 cut(s)	37, 333, 459, 625
Eam1104I	CTCTTC	1 cut(s)	499
EarI	CTCTTC	1 cut(s)	499
Eco130I	CCWWGG	1 cut(s)	371
Eco47I	GGWCC	3 cut(s)	125, 464, 1015
Eco57I	CTGAAG	1 cut(s)	384
Eco81I	CCTNAGG	1 cut(s)	459
EcoRII	CCWGG	5 cut(s)	110, 121, 190, 451, 718
EcoT14I	CCWWGG	1 cut(s)	371
ErhI	CCWWGG	1 cut(s)	371
Esp3I	CGTCTC	1 cut(s)	878
FaeI	CATG	7 cut(s)	100, 326, 375, 583, 890, 1015, 1039
FalI	AAGNNNNNCTT	4 cut(s)	806, 838, 968, 1000
FaqI	GGGAC	3 cut(s)	477, 600, 894
FatI	CATG	7 cut(s)	96, 322, 371, 579, 886, 1011, 1035
FauI	CCCGC	1 cut(s)	286
FauNDI	CATATG	1 cut(s)	71
Fnu4HI	GCNGC	3 cut(s)	327, 330, 630
FokI	GGATG	3 cut(s)	248, 425, 444
Fsp4HI	GCNGC	3 cut(s)	327, 330, 630
GluI	GCNGC	3 cut(s)	327, 330, 630
GsuI	CTGGAG	1 cut(s)	273
HaeIII	GGCC	5 cut(s)	424, 436, 456, 609, 1000
HapII	CCGG	1 cut(s)	584
HgaI	GACGC	1 cut(s)	857
Hin1II	CATG	7 cut(s)	100, 326, 375, 583, 890, 1015, 1039
HincII	GTYRAC	1 cut(s)	25
HindII	GTYRAC	1 cut(s)	25
HindIII	AAGCTT	1 cut(s)	815
HinfI	GANTC	4 cut(s)	4, 115, 381, 549
HpaI	GTTAAC	1 cut(s)	25
HpaII	CCGG	1 cut(s)	584
HphI	GGTGA	2 cut(s)	734, 866
Hpy166II	GTNNAC	2 cut(s)	25, 418
Hpy188I	TCNGA	2 cut(s)	40, 947
Hpy188III	TCNNGA	4 cut(s)	197, 799, 811, 1060
Hpy8I	GTNNAC	2 cut(s)	25, 418
Hpy99I	CGWCG	1 cut(s)	873
HpyAV	CCTTC	6 cut(s)	403, 773, 796, 851, 965, 970
HpyCH4III	ACNGT	5 cut(s)	47, 203, 758, 854, 932
HpyCH4IV	ACGT	2 cut(s)	955, 1023
HpyCH4V	TGCA	5 cut(s)	14, 406, 477, 938, 1043
HpyF10VI	GCNNNNNNNGC	1 cut(s)	453
HpyF3I	CTNAG	4 cut(s)	37, 333, 459, 625
HpySE526I	ACGT	2 cut(s)	955, 1023
Hsp92II	CATG	7 cut(s)	100, 326, 375, 583, 890, 1015, 1039
KspAI	GTTAAC	1 cut(s)	25
LmnI	GCTCC	3 cut(s)	193, 485, 734
Lsp1109I	GCAGC	2 cut(s)	313, 616
LweI	GCATC	3 cut(s)	268, 508, 947
MaeII	ACGT	2 cut(s)	955, 1023
MaeIII	GTNAC	5 cut(s)	130, 653, 722, 956, 1027
MboII	GAAGA	2 cut(s)	516, 788
MflI	RGATCY	3 cut(s)	16, 238, 336
MhlI	GDGCHC	1 cut(s)	745
MluCI	AATT	2 cut(s)	228, 751
MlyI	GAGTC	2 cut(s)	13, 109
MseI	TTAA	1 cut(s)	24
MspA1I	CMGCKG	2 cut(s)	293, 629
MspI	CCGG	1 cut(s)	584
MspR9I	CCNGG	6 cut(s)	112, 123, 192, 453, 585, 720
MvaI	CCWGG	5 cut(s)	112, 123, 192, 453, 720
MwoI	GCNNNNNNNGC	1 cut(s)	453
NciI	CCSGG	1 cut(s)	585
NcoI	CCATGG	1 cut(s)	371
NdeI	CATATG	1 cut(s)	71
NlaIII	CATG	7 cut(s)	100, 326, 375, 583, 890, 1015, 1039
NlaIV	GGNNCC	3 cut(s)	465, 1017, 1064
NmuCI	GTSAC	3 cut(s)	130, 722, 956
NspI	RCATGY	1 cut(s)	326
PfeI	GAWTC	2 cut(s)	381, 549
PflMI	CCANNNNNTGG	1 cut(s)	377
PkrI	GCNGC	3 cut(s)	328, 331, 631
Ple19I	CGATCG	1 cut(s)	810
PleI	GAGTC	2 cut(s)	12, 109
PpsI	GAGTC	2 cut(s)	12, 109
Psp6I	CCWGG	5 cut(s)	110, 121, 190, 451, 718
PspGI	CCWGG	5 cut(s)	110, 121, 190, 451, 718
PspN4I	GGNNCC	3 cut(s)	465, 1017, 1064
PspPI	GGNCC	6 cut(s)	125, 435, 455, 464, 608, 1015
PstI	CTGCAG	1 cut(s)	479
PstNI	CAGNNNCTG	1 cut(s)	632
PsuI	RGATCY	3 cut(s)	16, 238, 336
PvuI	CGATCG	1 cut(s)	810
PvuII	CAGCTG	1 cut(s)	629
RsaI	GTAC	1 cut(s)	760
RsaNI	GTAC	1 cut(s)	759
SaqAI	TTAA	1 cut(s)	24
SatI	GCNGC	3 cut(s)	327, 330, 630
Sau96I	GGNCC	6 cut(s)	125, 435, 455, 464, 608, 1015
ScaI	AGTACT	1 cut(s)	760
SchI	GAGTC	2 cut(s)	13, 109
ScrFI	CCNGG	6 cut(s)	112, 123, 192, 453, 585, 720
SduI	GDGCHC	1 cut(s)	745
SfaNI	GCATC	3 cut(s)	268, 508, 947
SfcI	CTRYAG	1 cut(s)	475
SinI	GGWCC	3 cut(s)	125, 464, 1015
SmlI	CTYRAG	2 cut(s)	222, 823
SmoI	CTYRAG	2 cut(s)	222, 823
Sse9I	AATT	2 cut(s)	228, 751
SsiI	CCGC	3 cut(s)	293, 329, 846
StyD4I	CCNGG	6 cut(s)	110, 121, 190, 451, 583, 718
StyI	CCWWGG	1 cut(s)	371
TaaI	ACNGT	5 cut(s)	47, 203, 758, 854, 932
TaiI	ACGT	2 cut(s)	958, 1026
TaqI	TCGA	3 cut(s)	384, 810, 941
TaqII	GACCGA	2 cut(s)	149, 604
TasI	AATT	2 cut(s)	228, 751
TatI	WGTACW	1 cut(s)	758
TauI	GCSGC	1 cut(s)	332
TfiI	GAWTC	2 cut(s)	381, 549
Tru1I	TTAA	1 cut(s)	24
Tru9I	TTAA	1 cut(s)	24
TscAI	CASTG	4 cut(s)	52, 184, 208, 565
TseFI	GTSAC	3 cut(s)	130, 722, 956
TseI	GCWGC	2 cut(s)	326, 629
Tsp45I	GTSAC	3 cut(s)	130, 722, 956
TspDTI	ATGAA	2 cut(s)	651, 903
TspGWI	ACGGA	1 cut(s)	236
TspRI	CASTG	4 cut(s)	52, 184, 208, 565
Van91I	CCANNNNNTGG	1 cut(s)	377
VpaK11BI	GGWCC	3 cut(s)	125, 464, 1015
XceI	RCATGY	1 cut(s)	326
ZrmI	AGTACT	1 cut(s)	760

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.