Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0001207

Physical Location & Seq

Genomic Coordinates Forward (+)

35222000 .. 35225194

Gene Structure 3195 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG333800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGGATCTCAGAAGCAAAGGAAGGACTCTGCCAGGACCTGTTACTGACCCCTCAAAACTCCCCAAATGGAACTATGATGGATCTAGCACAGGTCAAGCTCCAGGGGAAGACAGTGAAGTCATCCTATACCCTCAAGCCATTTTCAAGGACCCATTTAGGAGAGGCAACAATATCTTGGTTATGTGTGATACTTATACTCCGGCTGGAGAACCAATTCCAACAAACAAGAGATGTACTGCAGCAAAGATATTCAGCCATCCTGATGTTGCTGCTGAAGAGCCCTGGTATGGTCTGGAGCAGGAGTACACCTTGTTGCAGAAAGATGTCAATTGGCCATTGGGATGGCCAATTGGTGGTTTTCCTGGTCCTCAGGGACCGTACTACTGTGCTGCCGGCGCTGACAAAGCATTTGGGCGTGACATTGTTGATTCTCATTACAAGGCCTGCTTGTATGCAGGCATCAACATCAGTGGCATCAACGGTGAAGTCATGCCAGGCCAGTGGGAGTTTCAAGTTGGTCCTTCAGTCGGCATCTCTGCGGGTGATGAATTATGGGTTGCTCGTTATATTTTGGAGAGGATCACAGAGATTGCTGGAGTGGTTCTTTCGTTTGATCCCAAGCCAATCCCTGGTGATTGGAACGGAGCTGGGGCTCACACAAATTACAGTACCAAATCCATGAGACACCAAGGAGGCTATGAAATCATCAAGAAAGCAATTGAGAAGCTGGGACTAAGGCACAAAGAACACATTGCCGCATATGGAGAAGGCAATGAACGCCGATTGACTGGAAGACATGAAACAGCTAACATTCACACCTTCAAATGGGGTGTTGCAGACCGTGGTGCCTCCATCCGCGTTGGCCGTGACACAGAGAAAGAAGGCAAGGGCTATTTTGAGGACAGGAGGCCTGCTTCGAACATGGATCCATATGTGGTGACTTCTATGATCGCCGAGACCACTATTCTGTGGAAGCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

326

Amino Acids

36.03

Weight (kDa)

6.27

Isoelectric Point (pI)

43.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N	PF03951	24 - 67	2.5e-08	Glutamine synthetase, beta-Grasp domain
Gln-synt_C	PF00120	95 - 224	1.5e-10	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48470 AT1G66200 AT1G66200 AT1G66200 AT3G17820 AT5G16570 AT5G16570 AT5G37600
fragaria_vesca	FvH4_1g30220 FvH4_3g32400 FvH4_4g36850 FvH4_5g17650
malus_domestica	MD09G1270600.v1.1 MD13G1006600.v1.1 MD14G1238700.v1.1 MD16G1001400.v1.1 MD17G1268700.v1.1
prunus_persica	Prupe.1G346600_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.5G236300_v2.0.a1
pyrus_communis	pycom09g18170 pycom14g19980 pycom17g26730
rosa_chinensis	RchiOBHm_Chr3g0491191 RchiOBHm_Chr4g0446601 RchiOBHm_Chr7g0178021
rosa_laevigata	RLG00000005481 RLG00000005647 RLG00000022854
rosa_multiflora	Rmu_sc0014162.1_g000003 Rmu_sc0015729.1_g000003 Rmu_sc0019011.1_g000005 Rmu_ssc0000144.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275280 Rroxscaffold_5G00386990 Rroxscaffold_6G00393090
rosa_rugosa	Rorug03G0247600 Rorug04G0372700 Rorug06G0412400 Rorug06G0412400
rosa_samantha	Rh3BG333800 Rh3DG331200 Rh4AG434300 Rh4BG443500 Rh4CG460800 Rh4DG442300 Rh7AG011900 Rh7BG011300 Rh7CG011900 Rh7DG011900
rosa_wichuraiana	Rw3G026210 Rw3G026250 Rw4G037130 Rw7G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	116
AccB1I	GGYRCC	1 cut(s)	845
AccB7I	CCANNNNNTGG	2 cut(s)	353, 629
AccII	CGCG	1 cut(s)	858
AciI	CCGC	3 cut(s)	539, 756, 856
AclWI	GGATC	6 cut(s)	12, 88, 587, 608, 920, 933
AcoI	YGGCCR	3 cut(s)	332, 344, 862
AcuI	CTGAAG	2 cut(s)	294, 507
AfaI	GTAC	4 cut(s)	235, 305, 380, 670
AfiI	CCNNNNNNNGG	5 cut(s)	287, 353, 527, 629, 825
AgsI	TTSAA	3 cut(s)	145, 512, 823
AjnI	CCWGG	6 cut(s)	31, 100, 281, 361, 493, 628
AluBI	AGCT	4 cut(s)	98, 647, 727, 806
AluI	AGCT	4 cut(s)	98, 647, 727, 806
Alw26I	GTCTC	2 cut(s)	676, 950
AlwI	GGATC	6 cut(s)	12, 88, 587, 608, 920, 933
AlwNI	CAGNNNCTG	1 cut(s)	38
AoxI	GGCC	6 cut(s)	332, 344, 441, 496, 862, 908
ApeKI	GCWGC	3 cut(s)	239, 269, 389
ArsI	GACNNNNNNTTYG	2 cut(s)	392, 424
Asp700I	GAANNNNTTC	1 cut(s)	213
AspLEI	GCGC	1 cut(s)	398
AspS9I	GGNCC	5 cut(s)	35, 148, 365, 374, 518
AsuHPI	GGTGA	4 cut(s)	494, 554, 644, 949
AsuII	TTCGAA	1 cut(s)	917
AvaII	GGWCC	5 cut(s)	35, 148, 365, 374, 518
AxyI	CCTNAGG	1 cut(s)	369
BalI	TGGCCA	2 cut(s)	334, 346
BamHI	GGATCC	1 cut(s)	925
BanI	GGYRCC	1 cut(s)	845
BanII	GRGCYC	2 cut(s)	282, 655
BbsI	GAAGAC	2 cut(s)	114, 799
BbvI	GCAGC	3 cut(s)	251, 256, 376
BccI	CCATC	4 cut(s)	71, 264, 336, 860
BceAI	ACGGC	1 cut(s)	849
BciT130I	CCWGG	6 cut(s)	33, 102, 283, 363, 495, 630
BcoDI	GTCTC	2 cut(s)	676, 950
BfaI	CTAG	1 cut(s)	84
BfmI	CTRYAG	1 cut(s)	237
BfoI	RGCGCY	1 cut(s)	399
BisI	GCNGC	4 cut(s)	240, 270, 390, 756
BlsI	GCNGC	4 cut(s)	241, 271, 391, 757
Bme1390I	CCNGG	6 cut(s)	33, 102, 283, 363, 495, 630
Bme18I	GGWCC	5 cut(s)	35, 148, 365, 374, 518
BmgT120I	GGNCC	5 cut(s)	35, 148, 365, 374, 518
BmiI	GGNNCC	4 cut(s)	150, 375, 847, 927
BmrFI	CCNGG	6 cut(s)	33, 102, 283, 363, 495, 630
BmsI	GCATC	3 cut(s)	468, 483, 540
BpiI	GAAGAC	2 cut(s)	114, 799
BpmI	CTGGAG	4 cut(s)	84, 225, 314, 615
Bpu14I	TTCGAA	1 cut(s)	917
BpuEI	CTTGAG	1 cut(s)	117
BsaI	GGTCTC	1 cut(s)	950
BsaJI	CCNNGG	5 cut(s)	101, 281, 628, 688, 841
BsaXI	ACNNNNNCTCC	4 cut(s)	181, 211, 287, 317
Bsc4I	CCNNNNNNNGG	5 cut(s)	287, 353, 527, 629, 825
Bse118I	RCCGGY	1 cut(s)	392
Bse1I	ACTGG	2 cut(s)	499, 793
Bse21I	CCTNAGG	1 cut(s)	369
Bse3DI	GCAATG	2 cut(s)	750, 778
BseBI	CCWGG	6 cut(s)	33, 102, 283, 363, 495, 630
BseDI	CCNNGG	5 cut(s)	101, 281, 628, 688, 841
BseGI	GGATG	4 cut(s)	120, 256, 347, 852
BseLI	CCNNNNNNNGG	5 cut(s)	287, 353, 527, 629, 825
BseMI	GCAATG	2 cut(s)	750, 778
BseMII	CTCAG	2 cut(s)	22, 383
BseNI	ACTGG	2 cut(s)	499, 793
BseXI	GCAGC	3 cut(s)	251, 256, 376
BseYI	CCCAGC	2 cut(s)	647, 727
Bsh1236I	CGCG	1 cut(s)	858
BshFI	GGCC	6 cut(s)	334, 346, 443, 498, 864, 910
BshNI	GGYRCC	1 cut(s)	845
BsiSI	CCGG	2 cut(s)	200, 393
BslFI	GGGAC	2 cut(s)	387, 744
BslI	CCNNNNNNNGG	5 cut(s)	287, 353, 527, 629, 825
BsmAI	GTCTC	2 cut(s)	676, 950
BsmFI	GGGAC	2 cut(s)	387, 744
BsnI	GGCC	6 cut(s)	334, 346, 443, 498, 864, 910
Bso31I	GGTCTC	1 cut(s)	950
Bsp119I	TTCGAA	1 cut(s)	917
Bsp1286I	GDGCHC	2 cut(s)	282, 655
Bsp143I	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
BspACI	CCGC	3 cut(s)	539, 756, 856
BspANI	GGCC	6 cut(s)	334, 346, 443, 498, 864, 910
BspCNI	CTCAG	2 cut(s)	21, 382
BspFNI	CGCG	1 cut(s)	858
BspLI	GGNNCC	4 cut(s)	150, 375, 847, 927
BspMAI	CTGCAG	1 cut(s)	241
BspPI	GGATC	6 cut(s)	12, 88, 587, 608, 920, 933
BspQI	GCTCTTC	1 cut(s)	270
BspT104I	TTCGAA	1 cut(s)	917
BspT107I	GGYRCC	1 cut(s)	845
BspTNI	GGTCTC	1 cut(s)	950
BsrDI	GCAATG	2 cut(s)	750, 778
BsrFI	RCCGGY	1 cut(s)	392
BsrI	ACTGG	2 cut(s)	499, 793
BssAI	RCCGGY	1 cut(s)	392
BssECI	CCNNGG	5 cut(s)	101, 281, 628, 688, 841
BssMI	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
BssT1I	CCWWGG	1 cut(s)	688
Bst2UI	CCWGG	6 cut(s)	33, 102, 283, 363, 495, 630
Bst4CI	ACNGT	6 cut(s)	113, 378, 386, 482, 668, 842
Bst6I	CTCTTC	1 cut(s)	270
BstBI	TTCGAA	1 cut(s)	917
BstC8I	GCNNGC	4 cut(s)	394, 445, 457, 912
BstDEI	CTNAG	3 cut(s)	8, 369, 734
BstDSI	CCRYGG	1 cut(s)	841
BstF5I	GGATG	4 cut(s)	120, 256, 347, 852
BstFNI	CGCG	1 cut(s)	858
BstH2I	RGCGCY	1 cut(s)	399
BstHHI	GCGC	1 cut(s)	398
BstKTI	GATC	6 cut(s)	7, 83, 582, 616, 928, 951
BstMAI	GTCTC	2 cut(s)	676, 950
BstMBI	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
BstMWI	GCNNNNNNNGC	3 cut(s)	395, 404, 777
BstNI	CCWGG	6 cut(s)	33, 102, 283, 363, 495, 630
BstSCI	CCNGG	6 cut(s)	31, 100, 281, 361, 493, 628
BstSFI	CTRYAG	1 cut(s)	237
BstUI	CGCG	1 cut(s)	858
BstV1I	GCAGC	3 cut(s)	251, 256, 376
BstV2I	GAAGAC	2 cut(s)	114, 799
BstX2I	RGATCY	3 cut(s)	4, 80, 925
BstXI	CCANNNNNNTGG	2 cut(s)	342, 501
BstYI	RGATCY	3 cut(s)	4, 80, 925
Bsu36I	CCTNAGG	1 cut(s)	369
BsuRI	GGCC	6 cut(s)	334, 346, 443, 498, 864, 910
BtgI	CCRYGG	1 cut(s)	841
BtsCI	GGATG	4 cut(s)	120, 256, 347, 852
BtsIMutI	CAGTG	3 cut(s)	118, 475, 506
Cac8I	GCNNGC	4 cut(s)	394, 445, 457, 912
CaiI	CAGNNNCTG	1 cut(s)	38
CfoI	GCGC	1 cut(s)	398
Cfr10I	RCCGGY	1 cut(s)	392
Cfr13I	GGNCC	5 cut(s)	35, 148, 365, 374, 518
Csp6I	GTAC	4 cut(s)	234, 304, 379, 669
CspCI	CAANNNNNGTGG	2 cut(s)	451, 486
CviAII	CATG	4 cut(s)	490, 679, 797, 922
CviQI	GTAC	4 cut(s)	234, 304, 379, 669
DdeI	CTNAG	3 cut(s)	8, 369, 734
DpnI	GATC	6 cut(s)	6, 82, 581, 615, 927, 950
DpnII	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
DrdI	GACNNNNNNGTC	1 cut(s)	116
DseDI	GACNNNNNNGTC	1 cut(s)	116
EaeI	YGGCCR	3 cut(s)	332, 344, 862
Eam1104I	CTCTTC	1 cut(s)	270
EarI	CTCTTC	1 cut(s)	270
Eco130I	CCWWGG	1 cut(s)	688
Eco147I	AGGCCT	2 cut(s)	443, 910
Eco24I	GRGCYC	2 cut(s)	282, 655
Eco31I	GGTCTC	1 cut(s)	950
Eco47I	GGWCC	5 cut(s)	35, 148, 365, 374, 518
Eco57I	CTGAAG	2 cut(s)	294, 507
Eco81I	CCTNAGG	1 cut(s)	369
EcoO109I	RGGNCCY	2 cut(s)	35, 148
EcoRII	CCWGG	6 cut(s)	31, 100, 281, 361, 493, 628
EcoT14I	CCWWGG	1 cut(s)	688
EcoT38I	GRGCYC	2 cut(s)	282, 655
ErhI	CCWWGG	1 cut(s)	688
FaeI	CATG	4 cut(s)	493, 682, 800, 925
FalI	AAGNNNNNCTT	2 cut(s)	431, 463
FaqI	GGGAC	2 cut(s)	387, 744
FatI	CATG	4 cut(s)	489, 678, 796, 921
FauI	CCCGC	1 cut(s)	532
FauNDI	CATATG	2 cut(s)	760, 931
Fnu4HI	GCNGC	4 cut(s)	240, 270, 390, 756
FokI	GGATG	4 cut(s)	107, 243, 354, 839
FriOI	GRGCYC	2 cut(s)	282, 655
Fsp4HI	GCNGC	4 cut(s)	240, 270, 390, 756
FspBI	CTAG	1 cut(s)	84
GlaI	GCGC	1 cut(s)	397
GluI	GCNGC	4 cut(s)	240, 270, 390, 756
GsaI	CCCAGC	2 cut(s)	651, 731
GsuI	CTGGAG	4 cut(s)	84, 225, 314, 615
HaeII	RGCGCY	1 cut(s)	399
HaeIII	GGCC	6 cut(s)	334, 346, 443, 498, 864, 910
HapII	CCGG	2 cut(s)	200, 393
HhaI	GCGC	1 cut(s)	398
Hin1II	CATG	4 cut(s)	493, 682, 800, 925
Hin6I	GCGC	1 cut(s)	396
HinP1I	GCGC	1 cut(s)	396
HinfI	GANTC	2 cut(s)	25, 428
HpaII	CCGG	2 cut(s)	200, 393
HphI	GGTGA	4 cut(s)	494, 554, 644, 949
Hpy166II	GTNNAC	1 cut(s)	306
Hpy188I	TCNGA	1 cut(s)	11
Hpy188III	TCNNGA	3 cut(s)	260, 293, 709
Hpy8I	GTNNAC	1 cut(s)	306
HpyAV	CCTTC	5 cut(s)	15, 531, 761, 829, 875
HpyCH4III	ACNGT	6 cut(s)	113, 378, 386, 482, 668, 842
HpyCH4V	TGCA	4 cut(s)	239, 316, 455, 836
HpyF10VI	GCNNNNNNNGC	3 cut(s)	395, 404, 777
HpyF3I	CTNAG	3 cut(s)	8, 369, 734
Hsp92II	CATG	4 cut(s)	493, 682, 800, 925
HspAI	GCGC	1 cut(s)	396
KroI	GCCGGC	1 cut(s)	392
KroNI	GCCGGC	1 cut(s)	394
Kzo9I	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
LguI	GCTCTTC	1 cut(s)	270
LmnI	GCTCC	3 cut(s)	103, 295, 644
Lsp1109I	GCAGC	3 cut(s)	251, 256, 376
LweI	GCATC	3 cut(s)	468, 483, 540
MaeI	CTAG	1 cut(s)	84
MaeIII	GTNAC	4 cut(s)	40, 416, 866, 937
MalI	GATC	6 cut(s)	6, 82, 581, 615, 927, 950
MboI	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
MboII	GAAGA	3 cut(s)	119, 287, 804
MfeI	CAATTG	3 cut(s)	328, 348, 717
MflI	RGATCY	3 cut(s)	4, 80, 925
MhlI	GDGCHC	2 cut(s)	282, 655
MlsI	TGGCCA	2 cut(s)	334, 346
MluCI	AATT	6 cut(s)	213, 328, 348, 548, 661, 717
MluNI	TGGCCA	2 cut(s)	334, 346
MlyI	GAGTC	1 cut(s)	19
MmeI	TCCRAC	1 cut(s)	242
MnlI	CCTC	9 cut(s)	61, 141, 155, 378, 570, 686, 859, 892, 900
Mox20I	TGGCCA	2 cut(s)	334, 346
MroNI	GCCGGC	1 cut(s)	392
MroXI	GAANNNNTTC	1 cut(s)	213
MscI	TGGCCA	2 cut(s)	334, 346
MslI	CAYNNNNRTG	2 cut(s)	261, 340
Msp20I	TGGCCA	2 cut(s)	334, 346
MspI	CCGG	2 cut(s)	200, 393
MspR9I	CCNGG	6 cut(s)	33, 102, 283, 363, 495, 630
MunI	CAATTG	3 cut(s)	328, 348, 717
MvaI	CCWGG	6 cut(s)	33, 102, 283, 363, 495, 630
MvnI	CGCG	1 cut(s)	858
MwoI	GCNNNNNNNGC	3 cut(s)	395, 404, 777
NaeI	GCCGGC	1 cut(s)	394
NdeI	CATATG	2 cut(s)	760, 931
NdeII	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
NgoMIV	GCCGGC	1 cut(s)	392
NlaIII	CATG	4 cut(s)	493, 682, 800, 925
NlaIV	GGNNCC	4 cut(s)	150, 375, 847, 927
NmeAIII	GCCGAG	1 cut(s)	979
NmuCI	GTSAC	3 cut(s)	416, 866, 937
NspV	TTCGAA	1 cut(s)	917
PceI	AGGCCT	2 cut(s)	443, 910
PciSI	GCTCTTC	1 cut(s)	270
PdiI	GCCGGC	1 cut(s)	394
PdmI	GAANNNNTTC	1 cut(s)	213
PfeI	GAWTC	1 cut(s)	428
PflMI	CCANNNNNTGG	2 cut(s)	353, 629
PkrI	GCNGC	4 cut(s)	241, 271, 391, 757
PleI	GAGTC	1 cut(s)	19
PpsI	GAGTC	1 cut(s)	19
PpuMI	RGGWCCY	2 cut(s)	35, 148
Psp5II	RGGWCCY	2 cut(s)	35, 148
Psp6I	CCWGG	6 cut(s)	31, 100, 281, 361, 493, 628
PspFI	CCCAGC	2 cut(s)	647, 727
PspGI	CCWGG	6 cut(s)	31, 100, 281, 361, 493, 628
PspN4I	GGNNCC	4 cut(s)	150, 375, 847, 927
PspPI	GGNCC	5 cut(s)	35, 148, 365, 374, 518
PspPPI	RGGWCCY	2 cut(s)	35, 148
PstI	CTGCAG	1 cut(s)	241
PstNI	CAGNNNCTG	1 cut(s)	38
PsuI	RGATCY	3 cut(s)	4, 80, 925
RsaI	GTAC	4 cut(s)	235, 305, 380, 670
RsaNI	GTAC	4 cut(s)	234, 304, 379, 669
RseI	CAYNNNNRTG	2 cut(s)	261, 340
SapI	GCTCTTC	1 cut(s)	270
SatI	GCNGC	4 cut(s)	240, 270, 390, 756
Sau3AI	GATC	6 cut(s)	4, 80, 579, 613, 925, 948
Sau96I	GGNCC	5 cut(s)	35, 148, 365, 374, 518
SchI	GAGTC	1 cut(s)	19
ScrFI	CCNGG	6 cut(s)	33, 102, 283, 363, 495, 630
SduI	GDGCHC	2 cut(s)	282, 655
SetI	ASST	8 cut(s)	40, 94, 100, 311, 649, 729, 808, 821
SfaNI	GCATC	3 cut(s)	468, 483, 540
SfcI	CTRYAG	1 cut(s)	237
SfuI	TTCGAA	1 cut(s)	917
SinI	GGWCC	5 cut(s)	35, 148, 365, 374, 518
SmiMI	CAYNNNNRTG	2 cut(s)	261, 340
SmlI	CTYRAG	1 cut(s)	132
SmoI	CTYRAG	1 cut(s)	132
Sse9I	AATT	6 cut(s)	213, 328, 348, 548, 661, 717
SseBI	AGGCCT	2 cut(s)	443, 910
SsiI	CCGC	3 cut(s)	539, 756, 856
SspMI	CTAG	1 cut(s)	84
StuI	AGGCCT	2 cut(s)	443, 910
StyD4I	CCNGG	6 cut(s)	31, 100, 281, 361, 493, 628
StyI	CCWWGG	1 cut(s)	688
TaaI	ACNGT	6 cut(s)	113, 378, 386, 482, 668, 842
TaqI	TCGA	1 cut(s)	917
TasI	AATT	6 cut(s)	213, 328, 348, 548, 661, 717
TatI	WGTACW	2 cut(s)	233, 303
TauI	GCSGC	1 cut(s)	758
TfiI	GAWTC	1 cut(s)	428
TscAI	CASTG	3 cut(s)	118, 475, 506
TseFI	GTSAC	3 cut(s)	416, 866, 937
TseI	GCWGC	3 cut(s)	239, 269, 389
Tsp45I	GTSAC	3 cut(s)	416, 866, 937
TspDTI	ATGAA	4 cut(s)	561, 714, 789, 813
TspGWI	ACGGA	1 cut(s)	657
TspRI	CASTG	3 cut(s)	118, 475, 506
Van91I	CCANNNNNTGG	2 cut(s)	353, 629
VpaK11BI	GGWCC	5 cut(s)	35, 148, 365, 374, 518
XmnI	GAANNNNTTC	1 cut(s)	213
XspI	CTAG	1 cut(s)	84

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.