Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0001207

Physical Location & Seq

Genomic Coordinates Reverse (-)

23935523 .. 23938440

Gene Structure 2918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g30220.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGTCTTTGCTCACAGATCTCATCAACCTCGACCTCTCTGACTCCACTGACAAGATCATCGCCGAGTACATATGGATTGGTGGATCTGGCATGGACCTCAGAAGCAAAGCAAGGACTTTGCCAGGACCTGTTAGTGACCCCTCCAAACTCCCCAAATGGAACTATGATGGGTCCAGCACAGGCCAAGCTCCAGGGGAGGACAGTGAGGTCATCCTCTACCCTCAAGCCATTTTCAAGGACCCATTTAGGAGAGGCAACAACATCTTGGTTATGTGTGATGCTTACACTCCGGCTGGAGAACCAATTCCAACCAACAAGAGAAATGCTGCAGCAAAGATATTCAGCCATCCCGGTGTTGTTGCGGAAGTGCCCTGGTATGGTCTGGAGCAAGAGTACACCTTGTTGCAGAAAGATGTGAACTGGCCCCTTGGCTGGCCAATTGGTGGTTTTCCTGGCCCTCAGGGACCGTACTACTGTGCTGCCGGCGCTGACAAGGCATTTGGGCGTGACATTGTTGATGCTCATTACAAGGCCTGCATTTATGCTGGAATCAACATCAGTGGCATTAACGGTGAAGTCATGCCAGGACAGTGGGAGTTTCAAGTTGGTCCTTCAGTTGGCATCTCTGCGGGTGATGAATTGTGGGTTGCTCGTTATATTTTGGAGAGGATCACAGAGATTGCTGGAGTGGTTCTTTCTTTTGATCCCAAGCCAATCCCTGGTGATTGGAATGGAGCTGGGGCTCACACAAATTACAGTACTAAATCCATGCGAAACGAAGGAGGCTATGAAATCATCAAGAAAGCAATTGAGAAGCTGGGACTAAGGCACAAAGAACACATTGCAGCATATGGAGAAGGCAACGAACGCCGTTTGACAGGAAAACATGAAACAGCTGACATTAATACCTTCAAATGGGGAGTTGCAAACCGTGGTGCTTCTATCCGTGTTGGGCGTGACACAGAGAAAGAGGGCAAGGGATACTTTGAAGACAGGAGGCCTGCTTCCAACATGGATCCATATGTGGTAACTTCTATGATTGCTGAGACCACTATTGTGTGGAAGCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

357

Amino Acids

39.05

Weight (kDa)

5.64

Isoelectric Point (pI)

36.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N	PF03951	54 - 97	1.9e-08	Glutamine synthetase, beta-Grasp domain
Gln-synt_C	PF00120	126 - 347	3e-13	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48470 AT1G66200 AT1G66200 AT1G66200 AT3G17820 AT5G16570 AT5G16570 AT5G37600
fragaria_vesca	FvH4_1g30220 FvH4_3g32400 FvH4_4g36850 FvH4_5g17650
malus_domestica	MD09G1270600.v1.1 MD13G1006600.v1.1 MD14G1238700.v1.1 MD16G1001400.v1.1 MD17G1268700.v1.1
prunus_persica	Prupe.1G346600_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.5G236300_v2.0.a1
pyrus_communis	pycom09g18170 pycom14g19980 pycom17g26730
rosa_chinensis	RchiOBHm_Chr3g0491191 RchiOBHm_Chr4g0446601 RchiOBHm_Chr7g0178021
rosa_laevigata	RLG00000005481 RLG00000005647 RLG00000022854
rosa_multiflora	Rmu_sc0014162.1_g000003 Rmu_sc0015729.1_g000003 Rmu_sc0019011.1_g000005 Rmu_ssc0000144.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275280 Rroxscaffold_5G00386990 Rroxscaffold_6G00393090
rosa_rugosa	Rorug03G0247600 Rorug04G0372700 Rorug06G0412400 Rorug06G0412400
rosa_samantha	Rh3BG333800 Rh3DG331200 Rh4AG434300 Rh4BG443500 Rh4CG460800 Rh4DG442300 Rh7AG011900 Rh7BG011300 Rh7CG011900 Rh7DG011900
rosa_wichuraiana	Rw3G026210 Rw3G026250 Rw4G037130 Rw7G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	206
AccB7I	CCANNNNNTGG	2 cut(s)	443, 719
AciI	CCGC	2 cut(s)	362, 629
AclWI	GGATC	5 cut(s)	91, 677, 698, 1010, 1023
AcoI	YGGCCR	1 cut(s)	434
AcuI	CTGAAG	1 cut(s)	597
AfaI	GTAC	4 cut(s)	68, 395, 470, 760
AfiI	CCNNNNNNNGG	6 cut(s)	377, 432, 443, 617, 719, 915
AgsI	TTSAA	4 cut(s)	235, 602, 913, 989
AjnI	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 718
AleI	CACNNNNGTG	1 cut(s)	1055
AluBI	AGCT	4 cut(s)	188, 737, 817, 896
AluI	AGCT	4 cut(s)	188, 737, 817, 896
Alw26I	GTCTC	1 cut(s)	1040
AlwI	GGATC	5 cut(s)	91, 677, 698, 1010, 1023
AlwNI	CAGNNNCTG	1 cut(s)	128
AoxI	GGCC	6 cut(s)	181, 422, 434, 454, 531, 998
ApeKI	GCWGC	4 cut(s)	326, 329, 479, 845
ArsI	GACNNNNNNTTYG	2 cut(s)	482, 514
AseI	ATTAAT	1 cut(s)	903
Asp700I	GAANNNNTTC	1 cut(s)	303
AspLEI	GCGC	1 cut(s)	488
AspS9I	GGNCC	8 cut(s)	94, 125, 171, 238, 423, 455, 464, 608
AsuC2I	CCSGG	1 cut(s)	351
AsuHPI	GGTGA	3 cut(s)	584, 644, 734
AvaII	GGWCC	6 cut(s)	94, 125, 171, 238, 464, 608
AxyI	CCTNAGG	1 cut(s)	459
BaeGI	GKGCMC	1 cut(s)	372
BalI	TGGCCA	1 cut(s)	436
BamHI	GGATCC	1 cut(s)	1015
BanII	GRGCYC	1 cut(s)	745
BbsI	GAAGAC	1 cut(s)	996
BbvI	GCAGC	4 cut(s)	313, 341, 466, 857
BccI	CCATC	2 cut(s)	161, 354
BceAI	ACGGC	1 cut(s)	855
BciT130I	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 720
BciVI	GTATCC	1 cut(s)	974
BcnI	CCSGG	1 cut(s)	351
BcoDI	GTCTC	1 cut(s)	1040
BfmI	CTRYAG	1 cut(s)	327
BfoI	RGCGCY	1 cut(s)	489
BfuI	GTATCC	1 cut(s)	974
BglII	AGATCT	1 cut(s)	16
BisI	GCNGC	4 cut(s)	327, 330, 480, 846
BlsI	GCNGC	4 cut(s)	328, 331, 481, 847
BmcAI	AGTACT	1 cut(s)	760
Bme1390I	CCNGG	7 cut(s)	123, 192, 351, 373, 453, 585, 720
Bme18I	GGWCC	6 cut(s)	94, 125, 171, 238, 464, 608
BmgT120I	GGNCC	8 cut(s)	94, 125, 171, 238, 423, 455, 464, 608
BmiI	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
BmrFI	CCNGG	7 cut(s)	123, 192, 351, 373, 453, 585, 720
BmsI	GCATC	3 cut(s)	268, 508, 630
BpiI	GAAGAC	1 cut(s)	996
BpmI	CTGGAG	4 cut(s)	174, 315, 404, 705
BpuEI	CTTGAG	1 cut(s)	207
BpuMI	CCSGG	1 cut(s)	351
BsaI	GGTCTC	1 cut(s)	1040
BsaJI	CCNNGG	5 cut(s)	191, 371, 427, 718, 931
BsaXI	ACNNNNNCTCC	2 cut(s)	377, 407
Bsc4I	CCNNNNNNNGG	6 cut(s)	377, 432, 443, 617, 719, 915
Bse118I	RCCGGY	1 cut(s)	482
Bse1I	ACTGG	1 cut(s)	425
Bse21I	CCTNAGG	1 cut(s)	459
Bse3DI	GCAATG	1 cut(s)	840
BseBI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 720
BseDI	CCNNGG	5 cut(s)	191, 371, 427, 718, 931
BseGI	GGATG	2 cut(s)	210, 346
BseLI	CCNNNNNNNGG	6 cut(s)	377, 432, 443, 617, 719, 915
BseMI	GCAATG	1 cut(s)	840
BseMII	CTCAG	3 cut(s)	112, 473, 1035
BseNI	ACTGG	1 cut(s)	425
BseSI	GKGCMC	1 cut(s)	372
BseXI	GCAGC	4 cut(s)	313, 341, 466, 857
BseYI	CCCAGC	2 cut(s)	737, 817
BshFI	GGCC	6 cut(s)	183, 424, 436, 456, 533, 1000
BsiSI	CCGG	3 cut(s)	290, 351, 483
BslFI	GGGAC	2 cut(s)	477, 834
BslI	CCNNNNNNNGG	6 cut(s)	377, 432, 443, 617, 719, 915
BsmAI	GTCTC	1 cut(s)	1040
BsmFI	GGGAC	2 cut(s)	477, 834
BsnI	GGCC	6 cut(s)	183, 424, 436, 456, 533, 1000
Bso31I	GGTCTC	1 cut(s)	1040
Bsp1286I	GDGCHC	2 cut(s)	372, 745
Bsp143I	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
BspACI	CCGC	2 cut(s)	362, 629
BspANI	GGCC	6 cut(s)	183, 424, 436, 456, 533, 1000
BspCNI	CTCAG	3 cut(s)	111, 472, 1036
BspLI	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
BspMAI	CTGCAG	1 cut(s)	331
BspPI	GGATC	5 cut(s)	91, 677, 698, 1010, 1023
BspTNI	GGTCTC	1 cut(s)	1040
BsrDI	GCAATG	1 cut(s)	840
BsrFI	RCCGGY	1 cut(s)	482
BsrI	ACTGG	1 cut(s)	425
BssAI	RCCGGY	1 cut(s)	482
BssECI	CCNNGG	5 cut(s)	191, 371, 427, 718, 931
BssMI	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
BssT1I	CCWWGG	1 cut(s)	427
Bst2UI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 720
Bst4CI	ACNGT	7 cut(s)	203, 468, 476, 572, 591, 758, 932
BstC8I	GCNNGC	4 cut(s)	434, 484, 535, 1002
BstDEI	CTNAG	4 cut(s)	98, 459, 824, 1044
BstDSI	CCRYGG	1 cut(s)	931
BstF5I	GGATG	2 cut(s)	210, 346
BstH2I	RGCGCY	1 cut(s)	489
BstHHI	GCGC	1 cut(s)	488
BstKTI	GATC	6 cut(s)	19, 57, 86, 672, 706, 1018
BstMAI	GTCTC	1 cut(s)	1040
BstMBI	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
BstMWI	GCNNNNNNNGC	3 cut(s)	485, 494, 867
BstNI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 720
BstSCI	CCNGG	7 cut(s)	121, 190, 349, 371, 451, 583, 718
BstSFI	CTRYAG	1 cut(s)	327
BstSLI	GKGCMC	1 cut(s)	372
BstV1I	GCAGC	4 cut(s)	313, 341, 466, 857
BstV2I	GAAGAC	1 cut(s)	996
BstX2I	RGATCY	3 cut(s)	16, 83, 1015
BstXI	CCANNNNNNTGG	1 cut(s)	591
BstYI	RGATCY	3 cut(s)	16, 83, 1015
Bsu36I	CCTNAGG	1 cut(s)	459
BsuI	GTATCC	1 cut(s)	974
BsuRI	GGCC	6 cut(s)	183, 424, 436, 456, 533, 1000
BtgI	CCRYGG	1 cut(s)	931
BtgZI	GCGATG	1 cut(s)	43
BtsCI	GGATG	2 cut(s)	210, 346
BtsIMutI	CAGTG	4 cut(s)	45, 208, 565, 596
Cac8I	GCNNGC	4 cut(s)	434, 484, 535, 1002
CaiI	CAGNNNCTG	1 cut(s)	128
CfoI	GCGC	1 cut(s)	488
Cfr10I	RCCGGY	1 cut(s)	482
Cfr13I	GGNCC	8 cut(s)	94, 125, 171, 238, 423, 455, 464, 608
Csp6I	GTAC	4 cut(s)	67, 394, 469, 759
CspCI	CAANNNNNGTGG	2 cut(s)	541, 576
CviAII	CATG	5 cut(s)	91, 580, 769, 887, 1012
CviQI	GTAC	4 cut(s)	67, 394, 469, 759
DdeI	CTNAG	4 cut(s)	98, 459, 824, 1044
DpnI	GATC	6 cut(s)	18, 56, 85, 671, 705, 1017
DpnII	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
EaeI	YGGCCR	1 cut(s)	434
Eco130I	CCWWGG	1 cut(s)	427
Eco147I	AGGCCT	2 cut(s)	533, 1000
Eco24I	GRGCYC	1 cut(s)	745
Eco31I	GGTCTC	1 cut(s)	1040
Eco47I	GGWCC	6 cut(s)	94, 125, 171, 238, 464, 608
Eco57I	CTGAAG	1 cut(s)	597
Eco81I	CCTNAGG	1 cut(s)	459
EcoO109I	RGGNCCY	2 cut(s)	125, 238
EcoRII	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 718
EcoT14I	CCWWGG	1 cut(s)	427
EcoT38I	GRGCYC	1 cut(s)	745
ErhI	CCWWGG	1 cut(s)	427
FaeI	CATG	5 cut(s)	94, 583, 772, 890, 1015
FalI	AAGNNNNNCTT	2 cut(s)	968, 1000
FaqI	GGGAC	2 cut(s)	477, 834
FatI	CATG	5 cut(s)	90, 579, 768, 886, 1011
FauI	CCCGC	1 cut(s)	622
FauNDI	CATATG	3 cut(s)	71, 850, 1021
Fnu4HI	GCNGC	4 cut(s)	327, 330, 480, 846
FokI	GGATG	2 cut(s)	197, 333
FriOI	GRGCYC	1 cut(s)	745
Fsp4HI	GCNGC	4 cut(s)	327, 330, 480, 846
GlaI	GCGC	1 cut(s)	487
GluI	GCNGC	4 cut(s)	327, 330, 480, 846
GsaI	CCCAGC	2 cut(s)	741, 821
GsuI	CTGGAG	4 cut(s)	174, 315, 404, 705
HaeII	RGCGCY	1 cut(s)	489
HaeIII	GGCC	6 cut(s)	183, 424, 436, 456, 533, 1000
HapII	CCGG	3 cut(s)	290, 351, 483
HhaI	GCGC	1 cut(s)	488
Hin1II	CATG	5 cut(s)	94, 583, 772, 890, 1015
Hin6I	GCGC	1 cut(s)	486
HinP1I	GCGC	1 cut(s)	486
HinfI	GANTC	2 cut(s)	41, 549
HpaII	CCGG	3 cut(s)	290, 351, 483
HphI	GGTGA	3 cut(s)	584, 644, 734
Hpy166II	GTNNAC	2 cut(s)	396, 418
Hpy188I	TCNGA	2 cut(s)	40, 101
Hpy188III	TCNNGA	2 cut(s)	383, 799
Hpy8I	GTNNAC	2 cut(s)	396, 418
HpyAV	CCTTC	4 cut(s)	621, 773, 851, 919
HpyCH4III	ACNGT	7 cut(s)	203, 468, 476, 572, 591, 758, 932
HpyCH4V	TGCA	5 cut(s)	329, 406, 537, 845, 926
HpyF10VI	GCNNNNNNNGC	3 cut(s)	485, 494, 867
HpyF3I	CTNAG	4 cut(s)	98, 459, 824, 1044
Hsp92II	CATG	5 cut(s)	94, 583, 772, 890, 1015
HspAI	GCGC	1 cut(s)	486
KroI	GCCGGC	1 cut(s)	482
KroNI	GCCGGC	1 cut(s)	484
Kzo9I	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
LmnI	GCTCC	3 cut(s)	193, 385, 734
Lsp1109I	GCAGC	4 cut(s)	313, 341, 466, 857
LweI	GCATC	3 cut(s)	268, 508, 630
MaeIII	GTNAC	4 cut(s)	134, 506, 956, 1027
MalI	GATC	6 cut(s)	18, 56, 85, 671, 705, 1017
MboI	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
MboII	GAAGA	1 cut(s)	1001
MfeI	CAATTG	2 cut(s)	438, 807
MflI	RGATCY	3 cut(s)	16, 83, 1015
MhlI	GDGCHC	2 cut(s)	372, 745
MlsI	TGGCCA	1 cut(s)	436
MluCI	AATT	5 cut(s)	303, 438, 638, 751, 807
MluNI	TGGCCA	1 cut(s)	436
MlyI	GAGTC	1 cut(s)	35
MmeI	TCCRAC	2 cut(s)	332, 1032
Mox20I	TGGCCA	1 cut(s)	436
MroNI	GCCGGC	1 cut(s)	482
MroXI	GAANNNNTTC	1 cut(s)	303
MscI	TGGCCA	1 cut(s)	436
MseI	TTAA	2 cut(s)	567, 903
MslI	CAYNNNNRTG	2 cut(s)	351, 1055
Msp20I	TGGCCA	1 cut(s)	436
MspA1I	CMGCKG	1 cut(s)	896
MspI	CCGG	3 cut(s)	290, 351, 483
MspR9I	CCNGG	7 cut(s)	123, 192, 351, 373, 453, 585, 720
MunI	CAATTG	2 cut(s)	438, 807
MvaI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 720
MwoI	GCNNNNNNNGC	3 cut(s)	485, 494, 867
NaeI	GCCGGC	1 cut(s)	484
NciI	CCSGG	1 cut(s)	351
NdeI	CATATG	3 cut(s)	71, 850, 1021
NdeII	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
NgoMIV	GCCGGC	1 cut(s)	482
NlaIII	CATG	5 cut(s)	94, 583, 772, 890, 1015
NlaIV	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
NmeAIII	GCCGAG	1 cut(s)	88
NmuCI	GTSAC	3 cut(s)	134, 506, 956
OliI	CACNNNNGTG	1 cut(s)	1055
PceI	AGGCCT	2 cut(s)	533, 1000
PdiI	GCCGGC	1 cut(s)	484
PdmI	GAANNNNTTC	1 cut(s)	303
PfeI	GAWTC	1 cut(s)	549
PflMI	CCANNNNNTGG	2 cut(s)	443, 719
PkrI	GCNGC	4 cut(s)	328, 331, 481, 847
PleI	GAGTC	1 cut(s)	35
PpsI	GAGTC	1 cut(s)	35
PpuMI	RGGWCCY	2 cut(s)	125, 238
PshBI	ATTAAT	1 cut(s)	903
Psp5II	RGGWCCY	2 cut(s)	125, 238
Psp6I	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 718
PspFI	CCCAGC	2 cut(s)	737, 817
PspGI	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 718
PspN4I	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
PspPI	GGNCC	8 cut(s)	94, 125, 171, 238, 423, 455, 464, 608
PspPPI	RGGWCCY	2 cut(s)	125, 238
PstI	CTGCAG	1 cut(s)	331
PstNI	CAGNNNCTG	1 cut(s)	128
PsuI	RGATCY	3 cut(s)	16, 83, 1015
PvuII	CAGCTG	1 cut(s)	896
RsaI	GTAC	4 cut(s)	68, 395, 470, 760
RsaNI	GTAC	4 cut(s)	67, 394, 469, 759
RseI	CAYNNNNRTG	2 cut(s)	351, 1055
SaqAI	TTAA	2 cut(s)	567, 903
SatI	GCNGC	4 cut(s)	327, 330, 480, 846
Sau3AI	GATC	6 cut(s)	16, 54, 83, 669, 703, 1015
Sau96I	GGNCC	8 cut(s)	94, 125, 171, 238, 423, 455, 464, 608
ScaI	AGTACT	1 cut(s)	760
SchI	GAGTC	1 cut(s)	35
ScrFI	CCNGG	7 cut(s)	123, 192, 351, 373, 453, 585, 720
SduI	GDGCHC	2 cut(s)	372, 745
SfaNI	GCATC	3 cut(s)	268, 508, 630
SfcI	CTRYAG	1 cut(s)	327
SinI	GGWCC	6 cut(s)	94, 125, 171, 238, 464, 608
SmiMI	CAYNNNNRTG	2 cut(s)	351, 1055
SmlI	CTYRAG	1 cut(s)	222
SmoI	CTYRAG	1 cut(s)	222
Sse9I	AATT	5 cut(s)	303, 438, 638, 751, 807
SseBI	AGGCCT	2 cut(s)	533, 1000
SsiI	CCGC	2 cut(s)	362, 629
StuI	AGGCCT	2 cut(s)	533, 1000
StyD4I	CCNGG	7 cut(s)	121, 190, 349, 371, 451, 583, 718
StyI	CCWWGG	1 cut(s)	427
TaaI	ACNGT	7 cut(s)	203, 468, 476, 572, 591, 758, 932
TaqI	TCGA	1 cut(s)	30
TasI	AATT	5 cut(s)	303, 438, 638, 751, 807
TatI	WGTACW	3 cut(s)	66, 393, 758
TfiI	GAWTC	1 cut(s)	549
Tru1I	TTAA	2 cut(s)	567, 903
Tru9I	TTAA	2 cut(s)	567, 903
TscAI	CASTG	4 cut(s)	52, 208, 565, 596
TseFI	GTSAC	3 cut(s)	134, 506, 956
TseI	GCWGC	4 cut(s)	326, 329, 479, 845
Tsp45I	GTSAC	3 cut(s)	134, 506, 956
TspDTI	ATGAA	3 cut(s)	651, 804, 903
TspGWI	ACGGA	1 cut(s)	935
TspRI	CASTG	4 cut(s)	52, 208, 565, 596
Van91I	CCANNNNNTGG	2 cut(s)	443, 719
VpaK11BI	GGWCC	6 cut(s)	94, 125, 171, 238, 464, 608
VspI	ATTAAT	1 cut(s)	903
XmnI	GAANNNNTTC	1 cut(s)	303
ZrmI	AGTACT	1 cut(s)	760

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.