Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001207

Physical Location & Seq

Genomic Coordinates Reverse (-)

32836020 .. 32839973

Gene Structure 3954 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1268700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGTCGCTGCTCACAGATCTCATCAACCTGGATCTCTCAGGCTCTACTAAGAAGATCATCGCAGAGTACATATGGATTGGTGGATCTGGAATGGATATCAGAAGCAAAGCAAGGACTCTGCCAGGACCAGTGAGTGATCCATCAAAGCTCCCCAAGTGGAATTATGATGGTTCCAGCACAGGTCAAGCTCCTGGAGAAGATAGTGAAGTTATCTTATACCCTCAAGCCATTTTCAAGGATCCATTCAGGAGAGGCAACAACATTTTGGTGATATGCGATACCTACACACCTGGTGGAGAACCGATTCCAACCAACAAGAGGGCTGCTGCTGCAAAGATTTTCAGTCATCCTGATGTTGTTGCTGAAGTACCCTGGTATGGAATTGAACAGGAGTACACCTTGTTGCAGAAAGATGTCAAATGGCCCCTTGGGTGGCCTGTTGGTGGCTATCCAGGCCCTCAGGGCCCATACTATTGTGCTGCTGGAGCAGACAAGGCCTTCGGACGAGACATTGTTGACTCGCACTACAAGGCCTGTCTCTATGCAGGCATTAACATTAGTGGAATAAACGGTGAAGTGATGCCTGGCCAGTGGGAGTTTCAAGTTGGCCCTTCTGTCGGCATATCAGCTGGAGATGAACTGTGGGCTGCTCGTTATATTTTGGAGAGGATCACTGAGATTGCTGGAGTGGTTCTGTCTTTTGATCCCAAGCCAATCCAGGGTGATTGGAACGGGGCTGGTGCTCACACAAACTACAGCACCAAGTCCATGAGAGAAGATGGAGGCTATGAAATTATCAAGAAGGCAATTGACAAGTTGGGACTCAGGCACAAGGAGCACATTGCTGCCTATGGAGAAGGCAATGAACGCCGTTTGACAGGAAGGCATGAAACTGCCGATATCAACACATTTAAATGGGGTGTTGCCAACCGCGGTGCTTCTATCCGTGTTGGTAGAGAGACAGAACAAGCTGGCAAGGGCTATTTCGAGGACAGGAGGCCTGCGTCGAACATGGATCCATATGTTGTCACTTCTATGATTGCAGAAACCACCATTCTGTTGAAGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

357

Amino Acids

38.97

Weight (kDa)

5.94

Isoelectric Point (pI)

38.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N	PF03951	51 - 97	1.1e-08	Glutamine synthetase, beta-Grasp domain
Gln-synt_C	PF00120	126 - 254	1.6e-10	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48470 AT1G66200 AT1G66200 AT1G66200 AT3G17820 AT5G16570 AT5G16570 AT5G37600
fragaria_vesca	FvH4_1g30220 FvH4_3g32400 FvH4_4g36850 FvH4_5g17650
malus_domestica	MD09G1270600.v1.1 MD13G1006600.v1.1 MD14G1238700.v1.1 MD16G1001400.v1.1 MD17G1268700.v1.1
prunus_persica	Prupe.1G346600_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.5G236300_v2.0.a1
pyrus_communis	pycom09g18170 pycom14g19980 pycom17g26730
rosa_chinensis	RchiOBHm_Chr3g0491191 RchiOBHm_Chr4g0446601 RchiOBHm_Chr7g0178021
rosa_laevigata	RLG00000005481 RLG00000005647 RLG00000022854
rosa_multiflora	Rmu_sc0014162.1_g000003 Rmu_sc0015729.1_g000003 Rmu_sc0019011.1_g000005 Rmu_ssc0000144.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275280 Rroxscaffold_5G00386990 Rroxscaffold_6G00393090
rosa_rugosa	Rorug03G0247600 Rorug04G0372700 Rorug06G0412400 Rorug06G0412400
rosa_samantha	Rh3BG333800 Rh3DG331200 Rh4AG434300 Rh4BG443500 Rh4CG460800 Rh4DG442300 Rh7AG011900 Rh7BG011300 Rh7CG011900 Rh7DG011900
rosa_wichuraiana	Rw3G026210 Rw3G026250 Rw4G037130 Rw7G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	933
AciI	CCGC	2 cut(s)	931, 933
AclWI	GGATC	9 cut(s)	39, 91, 131, 233, 246, 677, 698, 1010, 1023
AcoI	YGGCCR	1 cut(s)	586
AcuI	CTGAAG	1 cut(s)	384
AdeI	CACNNNGTG	1 cut(s)	293
AfaI	GTAC	3 cut(s)	68, 369, 395
AfiI	CCNNNNNNNGG	6 cut(s)	318, 377, 432, 443, 617, 719
AgsI	TTSAA	4 cut(s)	235, 386, 602, 1063
AjnI	CCWGG	8 cut(s)	27, 121, 190, 289, 371, 451, 583, 717
AluBI	AGCT	4 cut(s)	148, 188, 629, 971
AluI	AGCT	4 cut(s)	148, 188, 629, 971
Alw21I	GWGCWC	2 cut(s)	745, 840
Alw26I	GTCTC	3 cut(s)	501, 542, 953
AlwI	GGATC	9 cut(s)	39, 91, 131, 233, 246, 677, 698, 1010, 1023
AoxI	GGCC	9 cut(s)	422, 434, 454, 463, 495, 531, 586, 607, 998
ApaI	GGGCCC	1 cut(s)	467
ApeKI	GCWGC	7 cut(s)	7, 323, 326, 329, 479, 647, 845
ArsI	GACNNNNNNTTYG	2 cut(s)	482, 514
Asp700I	GAANNNNTTC	1 cut(s)	303
AspS9I	GGNCC	6 cut(s)	125, 423, 455, 463, 464, 608
AsuHPI	GGTGA	3 cut(s)	280, 584, 734
AvaII	GGWCC	1 cut(s)	125
AxyI	CCTNAGG	1 cut(s)	459
BaeGI	GKGCMC	1 cut(s)	467
BalI	TGGCCA	1 cut(s)	588
BamHI	GGATCC	2 cut(s)	238, 1015
BanII	GRGCYC	1 cut(s)	467
Bbv12I	GWGCWC	2 cut(s)	745, 840
BbvI	GCAGC	6 cut(s)	310, 313, 316, 466, 634, 832
BccI	CCATC	3 cut(s)	148, 161, 773
BceAI	ACGGC	1 cut(s)	855
BciT130I	CCWGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
BcoDI	GTCTC	3 cut(s)	501, 542, 953
BfmI	CTRYAG	1 cut(s)	754
BglI	GCCNNNNNGGC	1 cut(s)	462
BglII	AGATCT	1 cut(s)	16
BisI	GCNGC	7 cut(s)	8, 324, 327, 330, 480, 648, 846
BlsI	GCNGC	7 cut(s)	9, 325, 328, 331, 481, 649, 847
Bme1390I	CCNGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
Bme18I	GGWCC	1 cut(s)	125
BmgT120I	GGNCC	6 cut(s)	125, 423, 455, 463, 464, 608
BmiI	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
BmrFI	CCNGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
BmsI	GCATC	1 cut(s)	570
BpmI	CTGGAG	4 cut(s)	213, 504, 651, 705
BpuEI	CTTGAG	1 cut(s)	207
BsaJI	CCNNGG	4 cut(s)	371, 427, 718, 931
Bsc4I	CCNNNNNNNGG	6 cut(s)	318, 377, 432, 443, 617, 719
Bse1I	ACTGG	2 cut(s)	128, 589
Bse21I	CCTNAGG	1 cut(s)	459
Bse3DI	GCAATG	2 cut(s)	840, 868
BseBI	CCWGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
BseDI	CCNNGG	4 cut(s)	371, 427, 718, 931
BseGI	GGATG	1 cut(s)	346
BseLI	CCNNNNNNNGG	6 cut(s)	318, 377, 432, 443, 617, 719
BseMI	GCAATG	2 cut(s)	840, 868
BseMII	CTCAG	4 cut(s)	51, 473, 666, 838
BseNI	ACTGG	2 cut(s)	128, 589
BseSI	GKGCMC	1 cut(s)	467
BseXI	GCAGC	6 cut(s)	310, 313, 316, 466, 634, 832
Bsh1236I	CGCG	1 cut(s)	933
BshFI	GGCC	9 cut(s)	424, 436, 456, 465, 497, 533, 588, 609, 1000
BsiHKAI	GWGCWC	2 cut(s)	745, 840
BslFI	GGGAC	1 cut(s)	834
BslI	CCNNNNNNNGG	6 cut(s)	318, 377, 432, 443, 617, 719
BsmAI	GTCTC	3 cut(s)	501, 542, 953
BsmFI	GGGAC	1 cut(s)	834
BsnI	GGCC	9 cut(s)	424, 436, 456, 465, 497, 533, 588, 609, 1000
Bsp120I	GGGCCC	1 cut(s)	463
Bsp1286I	GDGCHC	3 cut(s)	467, 745, 840
Bsp143I	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
BspACI	CCGC	2 cut(s)	931, 933
BspANI	GGCC	9 cut(s)	424, 436, 456, 465, 497, 533, 588, 609, 1000
BspCNI	CTCAG	4 cut(s)	50, 472, 667, 837
BspFNI	CGCG	1 cut(s)	933
BspLI	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
BspPI	GGATC	9 cut(s)	39, 91, 131, 233, 246, 677, 698, 1010, 1023
BsrDI	GCAATG	2 cut(s)	840, 868
BsrI	ACTGG	2 cut(s)	128, 589
BssECI	CCNNGG	4 cut(s)	371, 427, 718, 931
BssMI	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
BssT1I	CCWWGG	1 cut(s)	427
Bst2UI	CCWGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
Bst4CI	ACNGT	2 cut(s)	572, 642
BstC8I	GCNNGC	3 cut(s)	547, 973, 1002
BstDEI	CTNAG	5 cut(s)	37, 48, 459, 675, 824
BstDSI	CCRYGG	1 cut(s)	931
BstF5I	GGATG	1 cut(s)	346
BstFNI	CGCG	1 cut(s)	933
BstKTI	GATC	9 cut(s)	19, 34, 57, 86, 139, 241, 672, 706, 1018
BstMAI	GTCTC	3 cut(s)	501, 542, 953
BstMBI	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
BstMWI	GCNNNNNNNGC	7 cut(s)	329, 453, 462, 485, 494, 835, 867
BstNI	CCWGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
BstSCI	CCNGG	8 cut(s)	27, 121, 190, 289, 371, 451, 583, 717
BstSFI	CTRYAG	1 cut(s)	754
BstSLI	GKGCMC	1 cut(s)	467
BstUI	CGCG	1 cut(s)	933
BstV1I	GCAGC	6 cut(s)	310, 313, 316, 466, 634, 832
BstX2I	RGATCY	5 cut(s)	16, 31, 83, 238, 1015
BstYI	RGATCY	5 cut(s)	16, 31, 83, 238, 1015
Bsu36I	CCTNAGG	1 cut(s)	459
BsuRI	GGCC	9 cut(s)	424, 436, 456, 465, 497, 533, 588, 609, 1000
BtgI	CCRYGG	1 cut(s)	931
BtgZI	GCGATG	1 cut(s)	43
BtsCI	GGATG	1 cut(s)	346
BtsIMutI	CAGTG	3 cut(s)	135, 596, 672
Cac8I	GCNNGC	3 cut(s)	547, 973, 1002
Cfr13I	GGNCC	6 cut(s)	125, 423, 455, 463, 464, 608
Cfr42I	CCGCGG	1 cut(s)	934
CseI	GACGC	1 cut(s)	993
CsiI	ACCWGGT	1 cut(s)	289
Csp6I	GTAC	3 cut(s)	67, 368, 394
CviAII	CATG	4 cut(s)	769, 887, 1012, 1068
CviQI	GTAC	3 cut(s)	67, 368, 394
DdeI	CTNAG	5 cut(s)	37, 48, 459, 675, 824
DpnI	GATC	9 cut(s)	18, 33, 56, 85, 138, 240, 671, 705, 1017
DpnII	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
DraI	TTTAAA	1 cut(s)	913
DraIII	CACNNNGTG	1 cut(s)	293
EaeI	YGGCCR	1 cut(s)	586
Eco130I	CCWWGG	1 cut(s)	427
Eco147I	AGGCCT	3 cut(s)	497, 533, 1000
Eco24I	GRGCYC	1 cut(s)	467
Eco32I	GATATC	2 cut(s)	97, 901
Eco47I	GGWCC	1 cut(s)	125
Eco57I	CTGAAG	1 cut(s)	384
Eco81I	CCTNAGG	1 cut(s)	459
EcoO109I	RGGNCCY	2 cut(s)	455, 463
EcoRII	CCWGG	8 cut(s)	27, 121, 190, 289, 371, 451, 583, 717
EcoRV	GATATC	2 cut(s)	97, 901
EcoT14I	CCWWGG	1 cut(s)	427
EcoT38I	GRGCYC	1 cut(s)	467
ErhI	CCWWGG	1 cut(s)	427
FaeI	CATG	4 cut(s)	772, 890, 1015, 1071
FaqI	GGGAC	1 cut(s)	834
FatI	CATG	4 cut(s)	768, 886, 1011, 1067
FauNDI	CATATG	2 cut(s)	71, 1021
Fnu4HI	GCNGC	7 cut(s)	8, 324, 327, 330, 480, 648, 846
FokI	GGATG	1 cut(s)	333
FriOI	GRGCYC	1 cut(s)	467
Fsp4HI	GCNGC	7 cut(s)	8, 324, 327, 330, 480, 648, 846
GluI	GCNGC	7 cut(s)	8, 324, 327, 330, 480, 648, 846
GsuI	CTGGAG	4 cut(s)	213, 504, 651, 705
HaeIII	GGCC	9 cut(s)	424, 436, 456, 465, 497, 533, 588, 609, 1000
HgaI	GACGC	1 cut(s)	993
Hin1II	CATG	4 cut(s)	772, 890, 1015, 1071
HincII	GTYRAC	1 cut(s)	517
HindII	GTYRAC	1 cut(s)	517
HinfI	GANTC	4 cut(s)	115, 304, 518, 822
HphI	GGTGA	3 cut(s)	280, 584, 734
Hpy166II	GTNNAC	2 cut(s)	396, 517
Hpy188I	TCNGA	2 cut(s)	101, 503
Hpy188III	TCNNGA	4 cut(s)	87, 247, 350, 799
Hpy8I	GTNNAC	2 cut(s)	396, 517
Hpy99I	CGWCG	1 cut(s)	1009
HpyAV	CCTTC	5 cut(s)	508, 621, 796, 851, 876
HpyCH4III	ACNGT	2 cut(s)	572, 642
HpyCH4V	TGCA	4 cut(s)	332, 406, 545, 1043
HpyF10VI	GCNNNNNNNGC	7 cut(s)	329, 453, 462, 485, 494, 835, 867
HpyF3I	CTNAG	5 cut(s)	37, 48, 459, 675, 824
Hsp92II	CATG	4 cut(s)	772, 890, 1015, 1071
KspI	CCGCGG	1 cut(s)	934
Kzo9I	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
LmnI	GCTCC	4 cut(s)	153, 193, 485, 835
Lsp1109I	GCAGC	6 cut(s)	310, 313, 316, 466, 634, 832
LweI	GCATC	1 cut(s)	570
MabI	ACCWGGT	1 cut(s)	289
MaeIII	GTNAC	1 cut(s)	1027
MalI	GATC	9 cut(s)	18, 33, 56, 85, 138, 240, 671, 705, 1017
MboI	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
MboII	GAAGA	3 cut(s)	64, 209, 788
MfeI	CAATTG	1 cut(s)	807
MflI	RGATCY	5 cut(s)	16, 31, 83, 238, 1015
MhlI	GDGCHC	3 cut(s)	467, 745, 840
MlsI	TGGCCA	1 cut(s)	588
MluCI	AATT	4 cut(s)	160, 381, 792, 807
MluNI	TGGCCA	1 cut(s)	588
MlyI	GAGTC	3 cut(s)	109, 512, 816
MmeI	TCCRAC	1 cut(s)	332
MnlI	CCTC	8 cut(s)	231, 245, 312, 468, 660, 776, 982, 990
Mox20I	TGGCCA	1 cut(s)	588
MroXI	GAANNNNTTC	1 cut(s)	303
MscI	TGGCCA	1 cut(s)	588
MseI	TTAA	2 cut(s)	552, 912
MslI	CAYNNNNRTG	4 cut(s)	266, 351, 913, 1034
Msp20I	TGGCCA	1 cut(s)	588
MspA1I	CMGCKG	2 cut(s)	629, 933
MspR9I	CCNGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
MunI	CAATTG	1 cut(s)	807
MvaI	CCWGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
MvnI	CGCG	1 cut(s)	933
MwoI	GCNNNNNNNGC	7 cut(s)	329, 453, 462, 485, 494, 835, 867
NdeI	CATATG	2 cut(s)	71, 1021
NdeII	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
NlaIII	CATG	4 cut(s)	772, 890, 1015, 1071
NlaIV	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
NmuCI	GTSAC	1 cut(s)	1027
PceI	AGGCCT	3 cut(s)	497, 533, 1000
PdmI	GAANNNNTTC	1 cut(s)	303
PfeI	GAWTC	1 cut(s)	304
PfoI	TCCNGGA	1 cut(s)	190
PkrI	GCNGC	7 cut(s)	9, 325, 328, 331, 481, 649, 847
PleI	GAGTC	3 cut(s)	109, 512, 816
PpsI	GAGTC	3 cut(s)	109, 512, 816
Psp6I	CCWGG	8 cut(s)	27, 121, 190, 289, 371, 451, 583, 717
PspGI	CCWGG	8 cut(s)	27, 121, 190, 289, 371, 451, 583, 717
PspN4I	GGNNCC	5 cut(s)	172, 240, 425, 465, 1017
PspOMI	GGGCCC	1 cut(s)	463
PspPI	GGNCC	6 cut(s)	125, 423, 455, 463, 464, 608
PsuI	RGATCY	5 cut(s)	16, 31, 83, 238, 1015
PvuII	CAGCTG	1 cut(s)	629
RsaI	GTAC	3 cut(s)	68, 369, 395
RsaNI	GTAC	3 cut(s)	67, 368, 394
RseI	CAYNNNNRTG	4 cut(s)	266, 351, 913, 1034
SacII	CCGCGG	1 cut(s)	934
SaqAI	TTAA	2 cut(s)	552, 912
SatI	GCNGC	7 cut(s)	8, 324, 327, 330, 480, 648, 846
Sau3AI	GATC	9 cut(s)	16, 31, 54, 83, 136, 238, 669, 703, 1015
Sau96I	GGNCC	6 cut(s)	125, 423, 455, 463, 464, 608
SchI	GAGTC	3 cut(s)	109, 512, 816
ScrFI	CCNGG	8 cut(s)	29, 123, 192, 291, 373, 453, 585, 719
SduI	GDGCHC	3 cut(s)	467, 745, 840
SetI	ASST	9 cut(s)	30, 150, 184, 190, 284, 292, 401, 631, 973
SexAI	ACCWGGT	1 cut(s)	289
SfaNI	GCATC	1 cut(s)	570
SfcI	CTRYAG	1 cut(s)	754
SfiI	GGCCNNNNNGGCC	1 cut(s)	462
Sfr303I	CCGCGG	1 cut(s)	934
SgrBI	CCGCGG	1 cut(s)	934
SinI	GGWCC	1 cut(s)	125
SmiI	ATTTAAAT	1 cut(s)	913
SmiMI	CAYNNNNRTG	4 cut(s)	266, 351, 913, 1034
SmlI	CTYRAG	1 cut(s)	222
SmoI	CTYRAG	1 cut(s)	222
Sse9I	AATT	4 cut(s)	160, 381, 792, 807
SseBI	AGGCCT	3 cut(s)	497, 533, 1000
SsiI	CCGC	2 cut(s)	931, 933
StuI	AGGCCT	3 cut(s)	497, 533, 1000
StyD4I	CCNGG	8 cut(s)	27, 121, 190, 289, 371, 451, 583, 717
StyI	CCWWGG	1 cut(s)	427
SwaI	ATTTAAAT	1 cut(s)	913
TaaI	ACNGT	2 cut(s)	572, 642
TaqI	TCGA	2 cut(s)	987, 1007
TasI	AATT	4 cut(s)	160, 381, 792, 807
TatI	WGTACW	2 cut(s)	66, 393
TfiI	GAWTC	1 cut(s)	304
Tru1I	TTAA	2 cut(s)	552, 912
Tru9I	TTAA	2 cut(s)	552, 912
TscAI	CASTG	3 cut(s)	135, 596, 679
TseFI	GTSAC	1 cut(s)	1027
TseI	GCWGC	7 cut(s)	7, 323, 326, 329, 479, 647, 845
Tsp45I	GTSAC	1 cut(s)	1027
TspDTI	ATGAA	4 cut(s)	651, 804, 879, 903
TspGWI	ACGGA	1 cut(s)	935
TspRI	CASTG	3 cut(s)	135, 596, 679
VpaK11BI	GGWCC	1 cut(s)	125
XmnI	GAANNNNTTC	1 cut(s)	303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.