Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0001207

Physical Location & Seq

Genomic Coordinates Forward (+)

65710726 .. 65714256

Gene Structure 3531 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00386990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGTCTCTCCTCTCTGATATCATCAACCTCAACCTCTCGGAATCCACCCAGAAGATCATCGCCGAATACATATGGATAGGTGGATCTGGTACGGACTTGAGAAGCAAAGCAAGGACTCTGCCTGGGCCGGTTTCAGAGCCAGCGAAGCTACCAAAGTGGAACTACGACGGTTCGAGCACAAACCAAGCTCCAGGGGACGATAGTGAAGTGATCTTATATCCCCAGGCTATTTTCAAGGACCCATTCAGAAGAGGGAACAATATCTTGGTGATATGCGATGCGTATACACCGGCCGGAGAGCCGATCCCTACCAACAAGAGGGCACCTGCTGCCAAGATATTCAGCCACCCCGACGTTGCCAAAGAAGAGCCTTGGTATGGAATTGAGCAGGAGTACACTCTTCTTCAGAAGGATACAAATTGGCCGCTTGGGTGGCCTATTGGTGGCTTCCCTGGTCCACAGGGGCCCTACTACTGTGGTGCAGGAGCAGACAAGGCCTTCGGGCGTGATATAGTTGATGCTCATTACAAGGCCTGCCTCTATGCTGGAATCAATATCAGTGGCATCAATGCTGAGGTTATGCCAGGGCAGTGGGAATTTCAAGTTGGTCCCACTGAAGGTATTGCTGCTGGTGATCAAATATGGGCTGCTCGATACATCCTTGAGAGAATCACAGAAATCGCAGGAGTTGTTCTTTCTTTGGACCCTAAACCAATCAAGGGTGACTGGAACGGTGCCGGTGCTCACTGTAACTACAGTACCAAGTCGATGAGAAATGATGGTGGGATTGAAGTAATCAAGACGGCCATTGAAAAACTGAGCTTGCGTCACAAGGAGCACATTGCTGCTTATGGAGAAGGCAACGAGAGGAGACTGACTGGTCACCATGAGACCGCTGACATCAACACTTTCTCTTGGGGAGTGGCGAATAGGGGTGCCTCCGTCCGTGTTGGTCGTGATACCGAAAAACAAGGCAAAGGGTATTTTGAGGACAGAAGGCCAGCGTCCAACATGGATCCCTATGTTGTGACTTCCATGATTGCCGAGACCACCATTCTCACCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

355

Amino Acids

38.87

Weight (kDa)

5.73

Isoelectric Point (pI)

42.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N	PF03951	52 - 97	7.9e-08	Glutamine synthetase, beta-Grasp domain
Gln-synt_C	PF00120	125 - 254	9.5e-11	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48470 AT1G66200 AT1G66200 AT1G66200 AT3G17820 AT5G16570 AT5G16570 AT5G37600
fragaria_vesca	FvH4_1g30220 FvH4_3g32400 FvH4_4g36850 FvH4_5g17650
malus_domestica	MD09G1270600.v1.1 MD13G1006600.v1.1 MD14G1238700.v1.1 MD16G1001400.v1.1 MD17G1268700.v1.1
prunus_persica	Prupe.1G346600_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.5G236300_v2.0.a1
pyrus_communis	pycom09g18170 pycom14g19980 pycom17g26730
rosa_chinensis	RchiOBHm_Chr3g0491191 RchiOBHm_Chr4g0446601 RchiOBHm_Chr7g0178021
rosa_laevigata	RLG00000005481 RLG00000005647 RLG00000022854
rosa_multiflora	Rmu_sc0014162.1_g000003 Rmu_sc0015729.1_g000003 Rmu_sc0019011.1_g000005 Rmu_ssc0000144.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275280 Rroxscaffold_5G00386990 Rroxscaffold_6G00393090
rosa_rugosa	Rorug03G0247600 Rorug04G0372700 Rorug06G0412400 Rorug06G0412400
rosa_samantha	Rh3BG333800 Rh3DG331200 Rh4AG434300 Rh4BG443500 Rh4CG460800 Rh4DG442300 Rh7AG011900 Rh7BG011300 Rh7CG011900 Rh7DG011900
rosa_wichuraiana	Rw3G026210 Rw3G026250 Rw4G037130 Rw7G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	334
AasI	GACNNNNNNGTC	1 cut(s)	879
Acc36I	ACCTGC	1 cut(s)	334
AccB1I	GGYRCC	3 cut(s)	322, 734, 935
AccI	GTMKAC	1 cut(s)	284
AciI	CCGC	2 cut(s)	425, 894
AclWI	GGATC	4 cut(s)	91, 298, 1010, 1023
AcoI	YGGCCR	3 cut(s)	291, 422, 804
AcsI	RAATTY	1 cut(s)	596
AcuI	CTGAAG	2 cut(s)	389, 636
AfaI	GTAC	3 cut(s)	91, 395, 760
AfiI	CCNNNNNNNGG	5 cut(s)	318, 377, 443, 617, 719
AgsI	TTSAA	4 cut(s)	235, 602, 791, 812
AjnI	CCWGG	5 cut(s)	121, 190, 222, 451, 583
AjuI	GAANNNNNNNTTGG	4 cut(s)	248, 280, 588, 620
AluBI	AGCT	3 cut(s)	148, 188, 822
AluI	AGCT	3 cut(s)	148, 188, 822
Alw21I	GWGCWC	3 cut(s)	179, 745, 840
Alw26I	GTCTC	4 cut(s)	9, 865, 884, 1040
AlwI	GGATC	4 cut(s)	91, 298, 1010, 1023
AoxI	GGCC	9 cut(s)	125, 291, 422, 434, 464, 495, 531, 804, 998
ApaI	GGGCCC	1 cut(s)	468
ApeKI	GCWGC	4 cut(s)	329, 626, 647, 845
ApoI	RAATTY	1 cut(s)	596
ArsI	GACNNNNNNTTYG	2 cut(s)	482, 514
AspS9I	GGNCC	7 cut(s)	125, 238, 455, 464, 465, 608, 703
AsuHPI	GGTGA	5 cut(s)	280, 644, 734, 875, 1051
AvaII	GGWCC	4 cut(s)	238, 455, 608, 703
BaeGI	GKGCMC	2 cut(s)	325, 468
BaeI	ACNNNNGTAYC	2 cut(s)	742, 775
BamHI	GGATCC	1 cut(s)	1015
BanI	GGYRCC	3 cut(s)	322, 734, 935
BanII	GRGCYC	1 cut(s)	468
Bbv12I	GWGCWC	3 cut(s)	179, 745, 840
BbvCI	CCTCAGC	1 cut(s)	573
BbvI	GCAGC	4 cut(s)	316, 613, 634, 832
BccI	CCATC	1 cut(s)	773
BceAI	ACGGC	1 cut(s)	819
BciT130I	CCWGG	5 cut(s)	123, 192, 224, 453, 585
BciVI	GTATCC	1 cut(s)	406
BclI	TGATCA	1 cut(s)	634
BcoDI	GTCTC	4 cut(s)	9, 865, 884, 1040
BfmI	CTRYAG	1 cut(s)	754
BfuAI	ACCTGC	1 cut(s)	334
BfuI	GTATCC	1 cut(s)	406
BisI	GCNGC	5 cut(s)	330, 425, 627, 648, 846
BlsI	GCNGC	5 cut(s)	331, 426, 628, 649, 847
Bme1390I	CCNGG	5 cut(s)	123, 192, 224, 453, 585
Bme18I	GGWCC	4 cut(s)	238, 455, 608, 703
BmgT120I	GGNCC	7 cut(s)	125, 238, 455, 464, 465, 608, 703
BmiI	GGNNCC	9 cut(s)	240, 324, 465, 466, 610, 705, 736, 937, 1017
BmrFI	CCNGG	5 cut(s)	123, 192, 224, 453, 585
BmsI	GCATC	3 cut(s)	268, 508, 573
BpmI	CTGGAG	1 cut(s)	174
Bpu10I	CCTNAGC	1 cut(s)	573
BpuEI	CTTGAG	2 cut(s)	118, 683
BsaI	GGTCTC	2 cut(s)	884, 1040
BsaJI	CCNNGG	6 cut(s)	122, 191, 222, 371, 451, 584
Bsc4I	CCNNNNNNNGG	5 cut(s)	318, 377, 443, 617, 719
Bse118I	RCCGGY	3 cut(s)	127, 289, 737
Bse1I	ACTGG	2 cut(s)	731, 883
Bse3DI	GCAATG	1 cut(s)	840
BseBI	CCWGG	5 cut(s)	123, 192, 224, 453, 585
BseDI	CCNNGG	6 cut(s)	122, 191, 222, 371, 451, 584
BseGI	GGATG	1 cut(s)	657
BseLI	CCNNNNNNNGG	5 cut(s)	318, 377, 443, 617, 719
BseMI	GCAATG	1 cut(s)	840
BseMII	CTCAG	2 cut(s)	564, 809
BseNI	ACTGG	2 cut(s)	731, 883
BseRI	GAGGAG	1 cut(s)	883
BseSI	GKGCMC	2 cut(s)	325, 468
BseX3I	CGGCCG	1 cut(s)	291
BseXI	GCAGC	4 cut(s)	316, 613, 634, 832
BsgI	GTGCAG	1 cut(s)	501
Bsh1285I	CGRYCG	1 cut(s)	294
BshFI	GGCC	9 cut(s)	127, 293, 424, 436, 466, 497, 533, 806, 1000
BshNI	GGYRCC	3 cut(s)	322, 734, 935
BsiEI	CGRYCG	1 cut(s)	294
BsiHKAI	GWGCWC	3 cut(s)	179, 745, 840
BsiSI	CCGG	4 cut(s)	128, 290, 294, 738
BslFI	GGGAC	2 cut(s)	209, 594
BslI	CCNNNNNNNGG	5 cut(s)	318, 377, 443, 617, 719
BsmAI	GTCTC	4 cut(s)	9, 865, 884, 1040
BsmFI	GGGAC	2 cut(s)	209, 594
BsnI	GGCC	9 cut(s)	127, 293, 424, 436, 466, 497, 533, 806, 1000
Bso31I	GGTCTC	2 cut(s)	884, 1040
Bsp120I	GGGCCC	1 cut(s)	464
Bsp1286I	GDGCHC	5 cut(s)	179, 325, 468, 745, 840
Bsp143I	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
BspACI	CCGC	2 cut(s)	425, 894
BspANI	GGCC	9 cut(s)	127, 293, 424, 436, 466, 497, 533, 806, 1000
BspCNI	CTCAG	2 cut(s)	565, 810
BspLI	GGNNCC	9 cut(s)	240, 324, 465, 466, 610, 705, 736, 937, 1017
BspMI	ACCTGC	1 cut(s)	334
BspPI	GGATC	4 cut(s)	91, 298, 1010, 1023
BspQI	GCTCTTC	1 cut(s)	360
BspT107I	GGYRCC	3 cut(s)	322, 734, 935
BspTNI	GGTCTC	2 cut(s)	884, 1040
BsrDI	GCAATG	1 cut(s)	840
BsrFI	RCCGGY	3 cut(s)	127, 289, 737
BsrI	ACTGG	2 cut(s)	731, 883
BssAI	RCCGGY	3 cut(s)	127, 289, 737
BssECI	CCNNGG	6 cut(s)	122, 191, 222, 371, 451, 584
BssMI	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
BssNAI	GTATAC	1 cut(s)	285
BssT1I	CCWWGG	1 cut(s)	371
Bst1107I	GTATAC	1 cut(s)	285
Bst2UI	CCWGG	5 cut(s)	123, 192, 224, 453, 585
Bst4CI	ACNGT	5 cut(s)	170, 476, 734, 749, 758
Bst6I	CTCTTC	3 cut(s)	244, 360, 405
BstAPI	GCANNNNNTGC	1 cut(s)	329
BstC8I	GCNNGC	4 cut(s)	141, 535, 824, 1002
BstDEI	CTNAG	2 cut(s)	573, 818
BstEII	GGTNACC	1 cut(s)	881
BstF5I	GGATG	1 cut(s)	657
BstKTI	GATC	6 cut(s)	57, 86, 213, 306, 637, 1018
BstMAI	GTCTC	4 cut(s)	9, 865, 884, 1040
BstMBI	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
BstMCI	CGRYCG	1 cut(s)	294
BstMWI	GCNNNNNNNGC	4 cut(s)	145, 329, 433, 494
BstNI	CCWGG	5 cut(s)	123, 192, 224, 453, 585
BstPI	GGTNACC	1 cut(s)	881
BstSCI	CCNGG	5 cut(s)	121, 190, 222, 451, 583
BstSFI	CTRYAG	1 cut(s)	754
BstSLI	GKGCMC	2 cut(s)	325, 468
BstV1I	GCAGC	4 cut(s)	316, 613, 634, 832
BstX2I	RGATCY	2 cut(s)	83, 1015
BstXI	CCANNNNNNTGG	1 cut(s)	591
BstYI	RGATCY	2 cut(s)	83, 1015
BstZ17I	GTATAC	1 cut(s)	285
BstZI	CGGCCG	1 cut(s)	291
BsuI	GTATCC	1 cut(s)	406
BsuRI	GGCC	9 cut(s)	127, 293, 424, 436, 466, 497, 533, 806, 1000
BtgZI	GCGATG	2 cut(s)	43, 291
BtsCI	GGATG	1 cut(s)	657
BtsI	GCAGTG	1 cut(s)	596
BtsIMutI	CAGTG	4 cut(s)	565, 596, 612, 745
BveI	ACCTGC	1 cut(s)	334
Cac8I	GCNNGC	4 cut(s)	141, 535, 824, 1002
Cfr10I	RCCGGY	3 cut(s)	127, 289, 737
Cfr13I	GGNCC	7 cut(s)	125, 238, 455, 464, 465, 608, 703
CseI	GACGC	2 cut(s)	815, 993
Csp6I	GTAC	3 cut(s)	90, 394, 759
CspCI	CAANNNNNGTGG	2 cut(s)	541, 576
CviAII	CATG	3 cut(s)	887, 1012, 1036
CviQI	GTAC	3 cut(s)	90, 394, 759
DdeI	CTNAG	2 cut(s)	573, 818
DpnI	GATC	6 cut(s)	56, 85, 212, 305, 636, 1017
DpnII	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
DrdI	GACNNNNNNGTC	1 cut(s)	879
DseDI	GACNNNNNNGTC	1 cut(s)	879
EaeI	YGGCCR	3 cut(s)	291, 422, 804
EagI	CGGCCG	1 cut(s)	291
Eam1104I	CTCTTC	3 cut(s)	244, 360, 405
EarI	CTCTTC	3 cut(s)	244, 360, 405
EclXI	CGGCCG	1 cut(s)	291
Eco130I	CCWWGG	1 cut(s)	371
Eco147I	AGGCCT	2 cut(s)	497, 533
Eco24I	GRGCYC	1 cut(s)	468
Eco31I	GGTCTC	2 cut(s)	884, 1040
Eco32I	GATATC	1 cut(s)	19
Eco47I	GGWCC	4 cut(s)	238, 455, 608, 703
Eco52I	CGGCCG	1 cut(s)	291
Eco57I	CTGAAG	2 cut(s)	389, 636
Eco91I	GGTNACC	1 cut(s)	881
EcoO109I	RGGNCCY	3 cut(s)	238, 464, 465
EcoO65I	GGTNACC	1 cut(s)	881
EcoRII	CCWGG	5 cut(s)	121, 190, 222, 451, 583
EcoRV	GATATC	1 cut(s)	19
EcoT14I	CCWWGG	1 cut(s)	371
EcoT38I	GRGCYC	1 cut(s)	468
ErhI	CCWWGG	1 cut(s)	371
FaeI	CATG	3 cut(s)	890, 1015, 1039
FaqI	GGGAC	2 cut(s)	209, 594
FatI	CATG	3 cut(s)	886, 1011, 1035
FauNDI	CATATG	1 cut(s)	71
FbaI	TGATCA	1 cut(s)	634
FblI	GTMKAC	1 cut(s)	284
Fnu4HI	GCNGC	5 cut(s)	330, 425, 627, 648, 846
FokI	GGATG	1 cut(s)	644
FriOI	GRGCYC	1 cut(s)	468
Fsp4HI	GCNGC	5 cut(s)	330, 425, 627, 648, 846
GluI	GCNGC	5 cut(s)	330, 425, 627, 648, 846
GsuI	CTGGAG	1 cut(s)	174
HaeIII	GGCC	9 cut(s)	127, 293, 424, 436, 466, 497, 533, 806, 1000
HapII	CCGG	4 cut(s)	128, 290, 294, 738
HgaI	GACGC	2 cut(s)	815, 993
Hin1II	CATG	3 cut(s)	890, 1015, 1039
HinfI	GANTC	4 cut(s)	41, 115, 549, 669
HpaII	CCGG	4 cut(s)	128, 290, 294, 738
HphI	GGTGA	5 cut(s)	280, 644, 734, 875, 1051
Hpy166II	GTNNAC	3 cut(s)	285, 396, 458
Hpy188I	TCNGA	5 cut(s)	16, 40, 136, 248, 408
Hpy188III	TCNNGA	2 cut(s)	799, 956
Hpy8I	GTNNAC	3 cut(s)	285, 396, 458
Hpy99I	CGWCG	2 cut(s)	170, 356
HpyAV	CCTTC	5 cut(s)	403, 508, 611, 851, 990
HpyCH4III	ACNGT	5 cut(s)	170, 476, 734, 749, 758
HpyCH4IV	ACGT	1 cut(s)	354
HpyCH4V	TGCA	1 cut(s)	482
HpyF10VI	GCNNNNNNNGC	4 cut(s)	145, 329, 433, 494
HpyF3I	CTNAG	2 cut(s)	573, 818
HpySE526I	ACGT	1 cut(s)	354
Hsp92II	CATG	3 cut(s)	890, 1015, 1039
Ksp22I	TGATCA	1 cut(s)	634
Kzo9I	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
LguI	GCTCTTC	1 cut(s)	360
LmnI	GCTCC	3 cut(s)	193, 485, 835
Lsp1109I	GCAGC	4 cut(s)	316, 613, 634, 832
LweI	GCATC	3 cut(s)	268, 508, 573
MaeII	ACGT	1 cut(s)	354
MaeIII	GTNAC	5 cut(s)	722, 749, 827, 881, 1027
MalI	GATC	6 cut(s)	56, 85, 212, 305, 636, 1017
MboI	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
MboII	GAAGA	5 cut(s)	64, 261, 377, 392, 395
MflI	RGATCY	2 cut(s)	83, 1015
MhlI	GDGCHC	5 cut(s)	179, 325, 468, 745, 840
MluCI	AATT	3 cut(s)	381, 418, 596
MlyI	GAGTC	1 cut(s)	109
MmeI	TCCRAC	1 cut(s)	1032
MspA1I	CMGCKG	1 cut(s)	896
MspI	CCGG	4 cut(s)	128, 290, 294, 738
MspR9I	CCNGG	5 cut(s)	123, 192, 224, 453, 585
MvaI	CCWGG	5 cut(s)	123, 192, 224, 453, 585
MwoI	GCNNNNNNNGC	4 cut(s)	145, 329, 433, 494
NdeI	CATATG	1 cut(s)	71
NdeII	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
NlaIII	CATG	3 cut(s)	890, 1015, 1039
NlaIV	GGNNCC	9 cut(s)	240, 324, 465, 466, 610, 705, 736, 937, 1017
NmuCI	GTSAC	4 cut(s)	722, 827, 881, 1027
PaqCI	CACCTGC	1 cut(s)	334
PceI	AGGCCT	2 cut(s)	497, 533
PciSI	GCTCTTC	1 cut(s)	360
PfeI	GAWTC	3 cut(s)	41, 549, 669
PkrI	GCNGC	5 cut(s)	331, 426, 628, 649, 847
PleI	GAGTC	1 cut(s)	109
PpsI	GAGTC	1 cut(s)	109
PpuMI	RGGWCCY	1 cut(s)	238
Psp5II	RGGWCCY	1 cut(s)	238
Psp6I	CCWGG	5 cut(s)	121, 190, 222, 451, 583
PspEI	GGTNACC	1 cut(s)	881
PspGI	CCWGG	5 cut(s)	121, 190, 222, 451, 583
PspN4I	GGNNCC	9 cut(s)	240, 324, 465, 466, 610, 705, 736, 937, 1017
PspOMI	GGGCCC	1 cut(s)	464
PspPI	GGNCC	7 cut(s)	125, 238, 455, 464, 465, 608, 703
PspPPI	RGGWCCY	1 cut(s)	238
PsuI	RGATCY	2 cut(s)	83, 1015
RsaI	GTAC	3 cut(s)	91, 395, 760
RsaNI	GTAC	3 cut(s)	90, 394, 759
SapI	GCTCTTC	1 cut(s)	360
SatI	GCNGC	5 cut(s)	330, 425, 627, 648, 846
Sau3AI	GATC	6 cut(s)	54, 83, 210, 303, 634, 1015
Sau96I	GGNCC	7 cut(s)	125, 238, 455, 464, 465, 608, 703
SchI	GAGTC	1 cut(s)	109
ScrFI	CCNGG	5 cut(s)	123, 192, 224, 453, 585
SduI	GDGCHC	5 cut(s)	179, 325, 468, 745, 840
SfaNI	GCATC	3 cut(s)	268, 508, 573
SfcI	CTRYAG	1 cut(s)	754
SinI	GGWCC	4 cut(s)	238, 455, 608, 703
SmlI	CTYRAG	2 cut(s)	97, 662
SmoI	CTYRAG	2 cut(s)	97, 662
Sse9I	AATT	3 cut(s)	381, 418, 596
SseBI	AGGCCT	2 cut(s)	497, 533
SsiI	CCGC	2 cut(s)	425, 894
StuI	AGGCCT	2 cut(s)	497, 533
StyD4I	CCNGG	5 cut(s)	121, 190, 222, 451, 583
StyI	CCWWGG	1 cut(s)	371
TaaI	ACNGT	5 cut(s)	170, 476, 734, 749, 758
TaiI	ACGT	1 cut(s)	357
TaqI	TCGA	3 cut(s)	173, 652, 767
TasI	AATT	3 cut(s)	381, 418, 596
TatI	WGTACW	1 cut(s)	393
TauI	GCSGC	1 cut(s)	427
TfiI	GAWTC	3 cut(s)	41, 549, 669
TscAI	CASTG	4 cut(s)	565, 596, 619, 752
TseFI	GTSAC	4 cut(s)	722, 827, 881, 1027
TseI	GCWGC	4 cut(s)	329, 626, 647, 845
Tsp45I	GTSAC	4 cut(s)	722, 827, 881, 1027
TspGWI	ACGGA	3 cut(s)	107, 931, 935
TspRI	CASTG	4 cut(s)	565, 596, 619, 752
VpaK11BI	GGWCC	4 cut(s)	238, 455, 608, 703
XapI	RAATTY	1 cut(s)	596
XmiI	GTMKAC	1 cut(s)	284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.