Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001207

Physical Location & Seq

Genomic Coordinates Reverse (-)

34482333 .. 34484909

Gene Structure 2577 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1270600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGTCGCTCGTCTCAGATCTCATCAACCTCGACCTCTCAGACAGCACTAAGAAGATCATCGCAGAGTACATATGGATTGGTGGATCTGGAATGGATATCAGAAGCAAAGCAAGGACTCTGCCAGGACCAGTGAGTGATCCTTCAAAGCTTCCCAAGTGGAATTATGATGGTTCCAGCACAAATCAAGCTCCTGGAGAAGATAGTGAGGTTATTTTATACCCTCAAGCCATTTTCAAGGATCCATTCAGGAGAGGCAACAACATTTTGGTGATATGCGATGCCTACACACCCGCTGGGGAACCAATTCCAACTAACAAGAGGGCTGCAGCTGCAAAGATTTTCAGCCATCCTGATGTTGTTGCTGAAGTAACCTGGTATGGAATTGAACAGGAGTACACCTTGCTGCAGAAAGATGTCAAGTGGCCACTCGGCTGGCCCGTTGGTGGTTTCCCAGGCCCTCAGGGACCGTACTATTGTGCAGCTGGCGCTGACAAGGCCTTCGGGCGCGACATTGTTGACGCACACTACAAGGCCTGCCTTTATGCTGGCATCAACATTAGTGGAATCAACGGTGAAGTGATGCCTGGCCAGTGGGAATTTCAAGTTGGCCCTTCTGTCGGCATATCTGCTGGAGATGAACTGTGGGCTGCTCGTTATATTTTGGAGAGGATCACCGAGATTGCTGGGGTGGTTCTGTCATTTGATCCCAAGCCAATCCAGGGTGATTGGAACGGGGCTGGTGCTCACACAAACTACAGCACCAAGTCCATGAGAGAAGATGGAGGCTATGAAGTTATCAAGAAGGCAATTGACAAGTTGGGACTCAGGCACAAGGAGCACATTGCTGCCTATGGAGAAGGCAATGAGCGCCGTTTGACAGGAAGGCATGAAACTGCTGATATCAATACATTCAAATGGGGTGTAGCCAACCGCGGTGCTTCTATCCGTGTTGGTAGAGACACAGAACAAGCTGGCAAGGGCTATTTCGAGGACAGGAGGCCTGCATCGAACATGGATCCATACATTGTCACATCTATGATTGCAGAAACCACCCTTCTGTTGAAGGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Gln-synt_C Gln-synt_N

Protein Analysis

357

Amino Acids

38.97

Weight (kDa)

5.78

Isoelectric Point (pI)

35.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N	PF03951	51 - 97	5.6e-08	Glutamine synthetase, beta-Grasp domain
Gln-synt_C	PF00120	127 - 254	2.4e-10	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48470 AT1G66200 AT1G66200 AT1G66200 AT3G17820 AT5G16570 AT5G16570 AT5G37600
fragaria_vesca	FvH4_1g30220 FvH4_3g32400 FvH4_4g36850 FvH4_5g17650
malus_domestica	MD09G1270600.v1.1 MD13G1006600.v1.1 MD14G1238700.v1.1 MD16G1001400.v1.1 MD17G1268700.v1.1
prunus_persica	Prupe.1G346600_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.3G166500_v2.0.a1 Prupe.5G236300_v2.0.a1
pyrus_communis	pycom09g18170 pycom14g19980 pycom17g26730
rosa_chinensis	RchiOBHm_Chr3g0491191 RchiOBHm_Chr4g0446601 RchiOBHm_Chr7g0178021
rosa_laevigata	RLG00000005481 RLG00000005647 RLG00000022854
rosa_multiflora	Rmu_sc0014162.1_g000003 Rmu_sc0015729.1_g000003 Rmu_sc0019011.1_g000005 Rmu_ssc0000144.1_g000004
rosa_roxburghii	Rroxscaffold_3G00275280 Rroxscaffold_5G00386990 Rroxscaffold_6G00393090
rosa_rugosa	Rorug03G0247600 Rorug04G0372700 Rorug06G0412400 Rorug06G0412400
rosa_samantha	Rh3BG333800 Rh3DG331200 Rh4AG434300 Rh4BG443500 Rh4CG460800 Rh4DG442300 Rh7AG011900 Rh7BG011300 Rh7CG011900 Rh7DG011900
rosa_wichuraiana	Rw3G026210 Rw3G026250 Rw4G037130 Rw7G000940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	507, 933
AciI	CCGC	3 cut(s)	291, 931, 933
AclWI	GGATC	8 cut(s)	91, 131, 233, 246, 677, 698, 1010, 1023
AcoI	YGGCCR	2 cut(s)	422, 586
AcsI	RAATTY	1 cut(s)	596
AcuI	CTGAAG	1 cut(s)	384
AfaI	GTAC	3 cut(s)	68, 395, 470
AfiI	CCNNNNNNNGG	3 cut(s)	443, 617, 719
AgsI	TTSAA	6 cut(s)	144, 235, 386, 602, 913, 1063
AjnI	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 717
AjuI	GAANNNNNNNTTGG	2 cut(s)	588, 620
AluBI	AGCT	5 cut(s)	148, 188, 329, 482, 971
AluI	AGCT	5 cut(s)	148, 188, 329, 482, 971
Alw21I	GWGCWC	2 cut(s)	745, 840
Alw26I	GTCTC	2 cut(s)	16, 951
AlwI	GGATC	8 cut(s)	91, 131, 233, 246, 677, 698, 1010, 1023
AoxI	GGCC	8 cut(s)	422, 434, 454, 495, 531, 586, 607, 998
ApeKI	GCWGC	7 cut(s)	323, 326, 329, 403, 479, 647, 845
ApoI	RAATTY	1 cut(s)	596
ArsI	GACNNNNNNTTYG	2 cut(s)	482, 514
Asp700I	GAANNNNTTC	1 cut(s)	303
AspLEI	GCGC	3 cut(s)	488, 507, 870
AspS9I	GGNCC	5 cut(s)	125, 435, 455, 464, 608
AsuHPI	GGTGA	4 cut(s)	280, 584, 664, 734
AvaII	GGWCC	2 cut(s)	125, 464
AxyI	CCTNAGG	1 cut(s)	459
BalI	TGGCCA	2 cut(s)	424, 588
BamHI	GGATCC	2 cut(s)	238, 1015
Bbv12I	GWGCWC	2 cut(s)	745, 840
BbvI	GCAGC	7 cut(s)	310, 316, 338, 390, 491, 634, 832
BccI	CCATC	3 cut(s)	161, 354, 773
BceAI	ACGGC	1 cut(s)	855
BciT130I	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 719
BcoDI	GTCTC	2 cut(s)	16, 951
BfmI	CTRYAG	3 cut(s)	324, 404, 754
BfoI	RGCGCY	2 cut(s)	489, 871
BglII	AGATCT	1 cut(s)	16
BisI	GCNGC	7 cut(s)	324, 327, 330, 404, 480, 648, 846
BlsI	GCNGC	7 cut(s)	325, 328, 331, 405, 481, 649, 847
Bme1390I	CCNGG	6 cut(s)	123, 192, 373, 453, 585, 719
Bme18I	GGWCC	2 cut(s)	125, 464
BmgT120I	GGNCC	5 cut(s)	125, 435, 455, 464, 608
BmiI	GGNNCC	5 cut(s)	172, 240, 300, 465, 1017
BmrFI	CCNGG	6 cut(s)	123, 192, 373, 453, 585, 719
BmsI	GCATC	4 cut(s)	268, 558, 570, 1013
BpmI	CTGGAG	2 cut(s)	213, 651
BpuEI	CTTGAG	1 cut(s)	207
BsaJI	CCNNGG	3 cut(s)	451, 718, 931
Bsc4I	CCNNNNNNNGG	3 cut(s)	443, 617, 719
Bse1I	ACTGG	2 cut(s)	128, 589
Bse21I	CCTNAGG	1 cut(s)	459
Bse3DI	GCAATG	2 cut(s)	840, 868
BseBI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 719
BseDI	CCNNGG	3 cut(s)	451, 718, 931
BseGI	GGATG	1 cut(s)	346
BseLI	CCNNNNNNNGG	3 cut(s)	443, 617, 719
BseMI	GCAATG	2 cut(s)	840, 868
BseMII	CTCAG	4 cut(s)	27, 51, 473, 838
BseNI	ACTGG	2 cut(s)	128, 589
BseXI	GCAGC	7 cut(s)	310, 316, 338, 390, 491, 634, 832
BseYI	CCCAGC	2 cut(s)	293, 683
BsgI	GTGCAG	1 cut(s)	498
Bsh1236I	CGCG	2 cut(s)	507, 933
BshFI	GGCC	8 cut(s)	424, 436, 456, 497, 533, 588, 609, 1000
BsiHKAI	GWGCWC	2 cut(s)	745, 840
BslFI	GGGAC	2 cut(s)	477, 834
BslI	CCNNNNNNNGG	3 cut(s)	443, 617, 719
BsmAI	GTCTC	2 cut(s)	16, 951
BsmBI	CGTCTC	1 cut(s)	16
BsmFI	GGGAC	2 cut(s)	477, 834
BsnI	GGCC	8 cut(s)	424, 436, 456, 497, 533, 588, 609, 1000
Bsp1286I	GDGCHC	2 cut(s)	745, 840
Bsp143I	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
BspACI	CCGC	3 cut(s)	291, 931, 933
BspANI	GGCC	8 cut(s)	424, 436, 456, 497, 533, 588, 609, 1000
BspCNI	CTCAG	4 cut(s)	26, 50, 472, 837
BspFNI	CGCG	2 cut(s)	507, 933
BspLI	GGNNCC	5 cut(s)	172, 240, 300, 465, 1017
BspMAI	CTGCAG	2 cut(s)	328, 408
BspPI	GGATC	8 cut(s)	91, 131, 233, 246, 677, 698, 1010, 1023
BsrDI	GCAATG	2 cut(s)	840, 868
BsrI	ACTGG	2 cut(s)	128, 589
BssECI	CCNNGG	3 cut(s)	451, 718, 931
BssMI	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
Bst2UI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 719
Bst4CI	ACNGT	3 cut(s)	468, 572, 642
BstC8I	GCNNGC	6 cut(s)	434, 484, 535, 547, 973, 1002
BstDEI	CTNAG	5 cut(s)	13, 37, 48, 459, 824
BstDSI	CCRYGG	1 cut(s)	931
BstF5I	GGATG	1 cut(s)	346
BstFNI	CGCG	2 cut(s)	507, 933
BstH2I	RGCGCY	2 cut(s)	489, 871
BstHHI	GCGC	3 cut(s)	488, 507, 870
BstKTI	GATC	8 cut(s)	19, 57, 86, 139, 241, 672, 706, 1018
BstMAI	GTCTC	2 cut(s)	16, 951
BstMBI	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
BstMWI	GCNNNNNNNGC	5 cut(s)	329, 485, 494, 835, 867
BstNI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 719
BstSCI	CCNGG	6 cut(s)	121, 190, 371, 451, 583, 717
BstSFI	CTRYAG	3 cut(s)	324, 404, 754
BstUI	CGCG	2 cut(s)	507, 933
BstV1I	GCAGC	7 cut(s)	310, 316, 338, 390, 491, 634, 832
BstX2I	RGATCY	4 cut(s)	16, 83, 238, 1015
BstXI	CCANNNNNNTGG	1 cut(s)	432
BstYI	RGATCY	4 cut(s)	16, 83, 238, 1015
Bsu36I	CCTNAGG	1 cut(s)	459
BsuRI	GGCC	8 cut(s)	424, 436, 456, 497, 533, 588, 609, 1000
BtgI	CCRYGG	1 cut(s)	931
BtgZI	GCGATG	2 cut(s)	43, 291
BtsCI	GGATG	1 cut(s)	346
BtsIMutI	CAGTG	2 cut(s)	135, 596
Cac8I	GCNNGC	6 cut(s)	434, 484, 535, 547, 973, 1002
CfoI	GCGC	3 cut(s)	488, 507, 870
Cfr13I	GGNCC	5 cut(s)	125, 435, 455, 464, 608
Cfr42I	CCGCGG	1 cut(s)	934
CseI	GACGC	1 cut(s)	527
CsiI	ACCWGGT	1 cut(s)	371
Csp6I	GTAC	3 cut(s)	67, 394, 469
CspCI	CAANNNNNGTGG	2 cut(s)	541, 576
CviAII	CATG	3 cut(s)	769, 887, 1012
CviQI	GTAC	3 cut(s)	67, 394, 469
DdeI	CTNAG	5 cut(s)	13, 37, 48, 459, 824
DpnI	GATC	8 cut(s)	18, 56, 85, 138, 240, 671, 705, 1017
DpnII	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
EaeI	YGGCCR	2 cut(s)	422, 586
Eco147I	AGGCCT	3 cut(s)	497, 533, 1000
Eco32I	GATATC	2 cut(s)	97, 901
Eco47I	GGWCC	2 cut(s)	125, 464
Eco57I	CTGAAG	1 cut(s)	384
Eco81I	CCTNAGG	1 cut(s)	459
EcoO109I	RGGNCCY	1 cut(s)	455
EcoRII	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 717
EcoRV	GATATC	2 cut(s)	97, 901
Esp3I	CGTCTC	1 cut(s)	16
FaeI	CATG	3 cut(s)	772, 890, 1015
FaqI	GGGAC	2 cut(s)	477, 834
FatI	CATG	3 cut(s)	768, 886, 1011
FauI	CCCGC	1 cut(s)	298
FauNDI	CATATG	1 cut(s)	71
Fnu4HI	GCNGC	7 cut(s)	324, 327, 330, 404, 480, 648, 846
FokI	GGATG	1 cut(s)	333
Fsp4HI	GCNGC	7 cut(s)	324, 327, 330, 404, 480, 648, 846
GlaI	GCGC	3 cut(s)	487, 506, 869
GluI	GCNGC	7 cut(s)	324, 327, 330, 404, 480, 648, 846
GsaI	CCCAGC	2 cut(s)	297, 687
GsuI	CTGGAG	2 cut(s)	213, 651
HaeII	RGCGCY	2 cut(s)	489, 871
HaeIII	GGCC	8 cut(s)	424, 436, 456, 497, 533, 588, 609, 1000
HgaI	GACGC	1 cut(s)	527
HhaI	GCGC	3 cut(s)	488, 507, 870
Hin1II	CATG	3 cut(s)	772, 890, 1015
Hin6I	GCGC	3 cut(s)	486, 505, 868
HinP1I	GCGC	3 cut(s)	486, 505, 868
HincII	GTYRAC	1 cut(s)	517
HindII	GTYRAC	1 cut(s)	517
HindIII	AAGCTT	1 cut(s)	146
HinfI	GANTC	3 cut(s)	115, 564, 822
HphI	GGTGA	4 cut(s)	280, 584, 664, 734
Hpy166II	GTNNAC	2 cut(s)	396, 517
Hpy188I	TCNGA	3 cut(s)	16, 40, 101
Hpy188III	TCNNGA	4 cut(s)	87, 247, 350, 799
Hpy8I	GTNNAC	2 cut(s)	396, 517
HpyAV	CCTTC	8 cut(s)	150, 508, 621, 796, 851, 876, 1057, 1064
HpyCH4III	ACNGT	3 cut(s)	468, 572, 642
HpyCH4V	TGCA	6 cut(s)	326, 332, 406, 479, 1004, 1043
HpyF10VI	GCNNNNNNNGC	5 cut(s)	329, 485, 494, 835, 867
HpyF3I	CTNAG	5 cut(s)	13, 37, 48, 459, 824
Hsp92II	CATG	3 cut(s)	772, 890, 1015
HspAI	GCGC	3 cut(s)	486, 505, 868
KspI	CCGCGG	1 cut(s)	934
Kzo9I	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
LmnI	GCTCC	2 cut(s)	193, 835
Lsp1109I	GCAGC	7 cut(s)	310, 316, 338, 390, 491, 634, 832
LweI	GCATC	4 cut(s)	268, 558, 570, 1013
MabI	ACCWGGT	1 cut(s)	371
MaeIII	GTNAC	2 cut(s)	367, 1027
MalI	GATC	8 cut(s)	18, 56, 85, 138, 240, 671, 705, 1017
MboI	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
MboII	GAAGA	3 cut(s)	64, 209, 788
MfeI	CAATTG	1 cut(s)	807
MflI	RGATCY	4 cut(s)	16, 83, 238, 1015
MhlI	GDGCHC	2 cut(s)	745, 840
MlsI	TGGCCA	2 cut(s)	424, 588
MluCI	AATT	5 cut(s)	160, 303, 381, 596, 807
MluNI	TGGCCA	2 cut(s)	424, 588
MlyI	GAGTC	2 cut(s)	109, 816
MmeI	TCCRAC	1 cut(s)	332
Mox20I	TGGCCA	2 cut(s)	424, 588
MroXI	GAANNNNTTC	1 cut(s)	303
MscI	TGGCCA	2 cut(s)	424, 588
MslI	CAYNNNNRTG	4 cut(s)	266, 351, 913, 1034
Msp20I	TGGCCA	2 cut(s)	424, 588
MspA1I	CMGCKG	4 cut(s)	293, 329, 482, 933
MspR9I	CCNGG	6 cut(s)	123, 192, 373, 453, 585, 719
MunI	CAATTG	1 cut(s)	807
MvaI	CCWGG	6 cut(s)	123, 192, 373, 453, 585, 719
MvnI	CGCG	2 cut(s)	507, 933
MwoI	GCNNNNNNNGC	5 cut(s)	329, 485, 494, 835, 867
NdeI	CATATG	1 cut(s)	71
NdeII	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
NlaIII	CATG	3 cut(s)	772, 890, 1015
NlaIV	GGNNCC	5 cut(s)	172, 240, 300, 465, 1017
NmeAIII	GCCGAG	1 cut(s)	408
NmuCI	GTSAC	1 cut(s)	1027
PceI	AGGCCT	3 cut(s)	497, 533, 1000
PcsI	WCGNNNNNNNCGW	1 cut(s)	435
PdmI	GAANNNNTTC	1 cut(s)	303
PfeI	GAWTC	1 cut(s)	564
PfoI	TCCNGGA	1 cut(s)	190
PkrI	GCNGC	7 cut(s)	325, 328, 331, 405, 481, 649, 847
PleI	GAGTC	2 cut(s)	109, 816
PpsI	GAGTC	2 cut(s)	109, 816
Psp6I	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 717
PspFI	CCCAGC	2 cut(s)	293, 683
PspGI	CCWGG	6 cut(s)	121, 190, 371, 451, 583, 717
PspN4I	GGNNCC	5 cut(s)	172, 240, 300, 465, 1017
PspPI	GGNCC	5 cut(s)	125, 435, 455, 464, 608
PstI	CTGCAG	2 cut(s)	328, 408
PsuI	RGATCY	4 cut(s)	16, 83, 238, 1015
PvuII	CAGCTG	2 cut(s)	329, 482
RsaI	GTAC	3 cut(s)	68, 395, 470
RsaNI	GTAC	3 cut(s)	67, 394, 469
RseI	CAYNNNNRTG	4 cut(s)	266, 351, 913, 1034
SacII	CCGCGG	1 cut(s)	934
SatI	GCNGC	7 cut(s)	324, 327, 330, 404, 480, 648, 846
Sau3AI	GATC	8 cut(s)	16, 54, 83, 136, 238, 669, 703, 1015
Sau96I	GGNCC	5 cut(s)	125, 435, 455, 464, 608
SchI	GAGTC	2 cut(s)	109, 816
ScrFI	CCNGG	6 cut(s)	123, 192, 373, 453, 585, 719
SduI	GDGCHC	2 cut(s)	745, 840
SexAI	ACCWGGT	1 cut(s)	371
SfaNI	GCATC	4 cut(s)	268, 558, 570, 1013
SfcI	CTRYAG	3 cut(s)	324, 404, 754
Sfr303I	CCGCGG	1 cut(s)	934
SgrBI	CCGCGG	1 cut(s)	934
SinI	GGWCC	2 cut(s)	125, 464
SmiMI	CAYNNNNRTG	4 cut(s)	266, 351, 913, 1034
SmlI	CTYRAG	1 cut(s)	222
SmoI	CTYRAG	1 cut(s)	222
Sse9I	AATT	5 cut(s)	160, 303, 381, 596, 807
SseBI	AGGCCT	3 cut(s)	497, 533, 1000
SsiI	CCGC	3 cut(s)	291, 931, 933
StuI	AGGCCT	3 cut(s)	497, 533, 1000
StyD4I	CCNGG	6 cut(s)	121, 190, 371, 451, 583, 717
TaaI	ACNGT	3 cut(s)	468, 572, 642
TaqI	TCGA	3 cut(s)	30, 987, 1007
TasI	AATT	5 cut(s)	160, 303, 381, 596, 807
TatI	WGTACW	2 cut(s)	66, 393
TfiI	GAWTC	1 cut(s)	564
TscAI	CASTG	2 cut(s)	135, 596
TseFI	GTSAC	1 cut(s)	1027
TseI	GCWGC	7 cut(s)	323, 326, 329, 403, 479, 647, 845
Tsp45I	GTSAC	1 cut(s)	1027
TspDTI	ATGAA	3 cut(s)	651, 804, 903
TspGWI	ACGGA	1 cut(s)	935
TspRI	CASTG	2 cut(s)	135, 596
VpaK11BI	GGWCC	2 cut(s)	125, 464
XapI	RAATTY	1 cut(s)	596
XmnI	GAANNNNTTC	1 cut(s)	303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.