AT2G03370

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001444
Physical Location & Seq
Reverse (-)
1024643 .. 1027132
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03370.1

Sequence Viewer

Length: 1359 bp
ATGGTTAGGAAACCGTCGTCGAAGATTTATTCGGTGGCAGCAGCTGCGTTGGTGATCGTTATATATGTTGTTTTCTGCTCCGTGTTTGATCGTCAATTGTTACAATCGTCAAGTTTATATCAAGGTACGTTACAACAACCATGGGAATCTAGAAGAATCAAGCAAGCCCCACAACAACCGCAAGAGACTACTTCGGCAACAATCACATGTGACCGATCCCACACTAATTACGATCTCTGCTCAATCAACGGCTCATGTAATCTGGACCTTAAAACCGGAACTTTGACTCTAATGGACCCAACATCAGCACCACTTGTCGAAAAGATCAGGCCATACCCAAAAAAGGCTGACAACTGGATCATGCCTAGGATCAGAGAGCTAACACTAACCTCAGGCCCATTGGGTCTGCCCCGATCATGTGACATAACACATGATTTGCCGGCGATTGTGTTCAGTGCCGGAGGTTACACCGGAAGCATCTATCACGATTTAATGGATGGTTTTATCCCGCTTTTCATCACGGCCAATTCGGTATATCCGGACCGTGATTTCATCCCGGTGGTAGTAAATGCAAAGGAATGGTGGATGCCAAAATACATCGATATTCTAGGTACGTTTAGCAAACATAAACCCATATTACTACTAGACAAAGAAAGTGTTGCTACTACACATTGCTTCACTTCAGCTATTGTGGGCCTAATTACACATTGGCCCATGACAATAGACCCAACCCAAATACCCAACTCCAAATCATTAGTGGACTTCCACAATCTTTTAGAAAAAGCTTTCACCACAAACATATCTACTCCTAAAACCCACAAGCCTCGTCTCATGTTGGTTAGTCGATATGGTAACATTGGTCGAGTGATATTAAACGAGCAAGAGATCAAAGAGATGCTTGAAGATGTCGGGTTCGAGGTAATCATATTTAGACCATCGAAAACGACGAATTTGAAAGAAGCATATAAACTTATCAAGTCTAGCCACGGAATGGTCGGTGTACATGGTGCGGCATTAACACACTTGTTGTTTCTTCGACCCGGTTCAATATTTGTTCAAGTTGTTCCGCTTGGGCTAGGTTGGGCGTCTAAACCGTGTTACGAATCGCCAGCTAAGACAATGAAGTTGGAGTATCTGGAGTATAAGGTGAATGTGGAAGAGAGCAGTTTGATAGAGAAGTATAACAGAGATGATTTGGTTTTGAAAGATCCTATTGCTTATAGAGGAATGGATTGGAATGCGACTAAAATGAAAGTATATTTAAAAGAACAAGATGTGAGTTTAGATGTGAATCGGTTTAGAAAACATATGAATGAAGCATATAAGAAAGCTAAAATATTTATGGACATAAACGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

452

Amino Acids

51.12

Weight (kDa)

8.91

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 246 - 351 1.6e-13 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 991
AccIII TCCGGA 1 cut(s) 538
AciI CCGC 4 cut(s) 179, 509, 1010, 1067
AclWI GGATC 4 cut(s) 210, 365, 377, 1202
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 949
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 1085
AfaI GTAC 3 cut(s) 127, 613, 1002
AfiI CCNNNNNNNGG 2 cut(s) 343, 991
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 5 cut(s) 902, 955, 1047, 1058, 1204
AluBI AGCT 6 cut(s) 44, 379, 686, 785, 1112, 1331
AluI AGCT 6 cut(s) 44, 379, 686, 785, 1112, 1331
Alw26I GTCTC 2 cut(s) 179, 833
AlwI GGATC 4 cut(s) 210, 365, 377, 1202
AlwNI CAGNNNCTG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 5 cut(s) 329, 394, 522, 694, 710
ApeKI GCWGC 3 cut(s) 38, 41, 44
ApoI RAATTY 1 cut(s) 949
AspA2I CCTAGG 1 cut(s) 365
AspS9I GGNCC 6 cut(s) 265, 295, 395, 541, 694, 711
AsuC2I CCSGG 2 cut(s) 557, 1041
AsuHPI GGTGA 3 cut(s) 64, 781, 1159
AvaII GGWCC 3 cut(s) 265, 295, 541
AvrII CCTAGG 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 391
BbvI GCAGC 3 cut(s) 31, 50, 53
BccI CCATC 2 cut(s) 491, 943
BceAI ACGGC 2 cut(s) 265, 537
BcnI CCSGG 2 cut(s) 557, 1041
BcoDI GTCTC 2 cut(s) 179, 833
BfaI CTAG 6 cut(s) 150, 366, 608, 644, 981, 1076
BisI GCNGC 4 cut(s) 39, 42, 45, 1011
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 4 cut(s) 40, 43, 46, 1012
Bme1390I CCNGG 2 cut(s) 557, 1041
Bme18I GGWCC 3 cut(s) 265, 295, 541
BmgT120I GGNCC 6 cut(s) 265, 295, 395, 541, 694, 711
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 2 cut(s) 557, 1041
BmsI GCATC 3 cut(s) 486, 576, 885
BpmI CTGGAG 1 cut(s) 1157
BpuMI CCSGG 2 cut(s) 557, 1041
Bsa29I ATCGAT 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 1290
BsaHI GRCGYC 1 cut(s) 1085
BsaJI CCNNGG 3 cut(s) 140, 365, 985
BsaWI WCCGGW 3 cut(s) 275, 470, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 991
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 359
Bse21I CCTNAGG 1 cut(s) 391
Bse3DI GCAATG 1 cut(s) 670
Bse8I GATNNNNATC 1 cut(s) 1290
BseAI TCCGGA 1 cut(s) 538
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 140, 365, 985
BseGI GGATG 3 cut(s) 502, 552, 591
BseJI GATNNNNATC 1 cut(s) 1290
BseLI CCNNNNNNNGG 2 cut(s) 343, 991
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 359
BseXI GCAGC 3 cut(s) 31, 50, 53
BshFI GGCC 5 cut(s) 331, 396, 524, 696, 712
BshVI ATCGAT 1 cut(s) 600
BsiSI CCGG 7 cut(s) 276, 440, 459, 471, 539, 557, 1041
BslI CCNNNNNNNGG 2 cut(s) 343, 991
BsmAI GTCTC 2 cut(s) 179, 833
BsmBI CGTCTC 1 cut(s) 833
BsmI GAATGC 1 cut(s) 1243
BsnI GGCC 5 cut(s) 331, 396, 524, 696, 712
Bsp13I TCCGGA 1 cut(s) 538
Bsp1407I TGTACA 1 cut(s) 1000
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 4 cut(s) 179, 509, 1010, 1067
BspANI GGCC 5 cut(s) 331, 396, 524, 696, 712
BspCNI CTCAG 1 cut(s) 404
BspDI ATCGAT 1 cut(s) 600
BspEI TCCGGA 1 cut(s) 538
BspLI GGNNCC 1 cut(s) 297
BspPI GGATC 4 cut(s) 210, 365, 377, 1202
BsrDI GCAATG 1 cut(s) 670
BsrFI RCCGGY 1 cut(s) 439
BsrGI TGTACA 1 cut(s) 1000
BsrI ACTGG 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 3 cut(s) 140, 365, 985
BssNI GRCGYC 1 cut(s) 1085
BssT1I CCWWGG 2 cut(s) 140, 365
Bst4CI ACNGT 4 cut(s) 15, 545, 1095, 1355
Bst6I CTCTTC 1 cut(s) 1152
BstACI GRCGYC 1 cut(s) 1085
BstAPI GCANNNNNTGC 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 1000
BstC8I GCNNGC 3 cut(s) 165, 441, 1110
BstDEI CTNAG 2 cut(s) 391, 1113
BstDSI CCRYGG 2 cut(s) 140, 985
BstF5I GGATG 3 cut(s) 502, 552, 591
BstMAI GTCTC 2 cut(s) 179, 833
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 2 cut(s) 555, 1039
BstV1I GCAGC 3 cut(s) 31, 50, 53
BstX2I RGATCY 1 cut(s) 1207
BstYI RGATCY 1 cut(s) 1207
Bsu15I ATCGAT 1 cut(s) 600
Bsu36I CCTNAGG 1 cut(s) 391
BsuRI GGCC 5 cut(s) 331, 396, 524, 696, 712
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 2 cut(s) 140, 985
BtsCI GGATG 3 cut(s) 502, 552, 591
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 3 cut(s) 165, 441, 1110
CaiI CAGNNNCTG 1 cut(s) 44
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 6 cut(s) 265, 295, 395, 541, 694, 711
ClaI ATCGAT 1 cut(s) 600
CpoI CGGWCCG 1 cut(s) 541
CseI GACGC 1 cut(s) 1074
Csp6I GTAC 3 cut(s) 126, 612, 1001
CspI CGGWCCG 1 cut(s) 541
CviAII CATG 9 cut(s) 141, 207, 255, 361, 417, 431, 715, 832, 1004
CviQI GTAC 3 cut(s) 126, 612, 1001
DdeI CTNAG 2 cut(s) 391, 1113
DraI TTTAAA 1 cut(s) 1263
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
Eco130I CCWWGG 2 cut(s) 140, 365
Eco47I GGWCC 3 cut(s) 265, 295, 541
Eco57I CTGAAG 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 391
EcoT14I CCWWGG 2 cut(s) 140, 365
ErhI CCWWGG 2 cut(s) 140, 365
Esp3I CGTCTC 1 cut(s) 833
FaeI CATG 9 cut(s) 144, 210, 258, 364, 420, 434, 718, 835, 1007
FalI AAGNNNNNCTT 2 cut(s) 882, 914
FatI CATG 9 cut(s) 140, 206, 254, 360, 416, 430, 714, 831, 1003
FauI CCCGC 1 cut(s) 516
FauNDI CATATG 1 cut(s) 1308
Fnu4HI GCNGC 4 cut(s) 39, 42, 45, 1011
FokI GGATG 3 cut(s) 509, 539, 598
Fsp4HI GCNGC 4 cut(s) 39, 42, 45, 1011
FspBI CTAG 6 cut(s) 150, 366, 608, 644, 981, 1076
GluI GCNGC 4 cut(s) 39, 42, 45, 1011
GsuI CTGGAG 1 cut(s) 1157
HaeIII GGCC 5 cut(s) 331, 396, 524, 696, 712
HapII CCGG 7 cut(s) 276, 440, 459, 471, 539, 557, 1041
HgaI GACGC 1 cut(s) 1074
Hin1I GRCGYC 1 cut(s) 1085
Hin1II CATG 9 cut(s) 144, 210, 258, 364, 420, 434, 718, 835, 1007
HindIII AAGCTT 1 cut(s) 783
HinfI GANTC 5 cut(s) 146, 156, 286, 1103, 1291
HpaII CCGG 7 cut(s) 276, 440, 459, 471, 539, 557, 1041
HphI GGTGA 3 cut(s) 64, 781, 1159
Hpy166II GTNNAC 2 cut(s) 760, 1001
Hpy188I TCNGA 1 cut(s) 374
Hpy188III TCNNGA 5 cut(s) 150, 263, 485, 539, 1136
Hpy8I GTNNAC 2 cut(s) 760, 1001
Hpy99I CGWCG 3 cut(s) 19, 22, 949
HpyCH4III ACNGT 4 cut(s) 15, 545, 1095, 1355
HpyCH4IV ACGT 2 cut(s) 128, 614
HpyCH4V TGCA 1 cut(s) 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 2 cut(s) 391, 1113
HpySE526I ACGT 2 cut(s) 128, 614
Hsp92I GRCGYC 1 cut(s) 1085
Hsp92II CATG 9 cut(s) 144, 210, 258, 364, 420, 434, 718, 835, 1007
Kpn2I TCCGGA 1 cut(s) 538
KroI GCCGGC 1 cut(s) 439
KroNI GCCGGC 1 cut(s) 441
LmnI GCTCC 1 cut(s) 83
Lsp1109I GCAGC 3 cut(s) 31, 50, 53
LweI GCATC 3 cut(s) 486, 576, 885
MaeI CTAG 6 cut(s) 150, 366, 608, 644, 981, 1076
MaeII ACGT 2 cut(s) 128, 614
MaeIII GTNAC 7 cut(s) 99, 129, 209, 419, 464, 851, 1097
MboII GAAGA 5 cut(s) 34, 165, 914, 1025, 1169
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 1 cut(s) 1207
MluCI AATT 5 cut(s) 95, 226, 526, 699, 949
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 1 cut(s) 1107
MnlI CCTC 5 cut(s) 400, 455, 834, 910, 1217
MroI TCCGGA 1 cut(s) 538
MroNI GCCGGC 1 cut(s) 439
MseI TTAA 5 cut(s) 270, 491, 872, 1016, 1262
MslI CAYNNNNRTG 2 cut(s) 557, 1311
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 7 cut(s) 276, 440, 459, 471, 539, 557, 1041
MspR9I CCNGG 2 cut(s) 557, 1041
MunI CAATTG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 1243
MwoI GCNNNNNNNGC 1 cut(s) 44
NaeI GCCGGC 1 cut(s) 441
NciI CCSGG 2 cut(s) 557, 1041
NcoI CCATGG 1 cut(s) 140
NdeI CATATG 1 cut(s) 1308
NgoMIV GCCGGC 1 cut(s) 439
NlaIII CATG 9 cut(s) 144, 210, 258, 364, 420, 434, 718, 835, 1007
NlaIV GGNNCC 1 cut(s) 297
NmuCI GTSAC 2 cut(s) 209, 419
NspI RCATGY 1 cut(s) 210
PciI ACATGT 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 944
PctI GAATGC 1 cut(s) 1243
PdiI GCCGGC 1 cut(s) 441
PfeI GAWTC 4 cut(s) 146, 156, 1103, 1291
PflMI CCANNNNNTGG 1 cut(s) 991
PkrI GCNGC 4 cut(s) 40, 43, 46, 1012
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PscI ACATGT 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 6 cut(s) 265, 295, 395, 541, 694, 711
PstNI CAGNNNCTG 1 cut(s) 44
PsuI RGATCY 1 cut(s) 1207
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 3 cut(s) 127, 613, 1002
RsaNI GTAC 3 cut(s) 126, 612, 1001
RseI CAYNNNNRTG 2 cut(s) 557, 1311
Rsr2I CGGWCCG 1 cut(s) 541
RsrII CGGWCCG 1 cut(s) 541
SaqAI TTAA 5 cut(s) 270, 491, 872, 1016, 1262
SatI GCNGC 4 cut(s) 39, 42, 45, 1011
Sau96I GGNCC 6 cut(s) 265, 295, 395, 541, 694, 711
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 557, 1041
SfaNI GCATC 3 cut(s) 486, 576, 885
SinI GGWCC 3 cut(s) 265, 295, 541
SmiMI CAYNNNNRTG 2 cut(s) 557, 1311
Sse9I AATT 5 cut(s) 95, 226, 526, 699, 949
SsiI CCGC 4 cut(s) 179, 509, 1010, 1067
SspI AATATT 2 cut(s) 1050, 1338
SspMI CTAG 6 cut(s) 150, 366, 608, 644, 981, 1076
StyD4I CCNGG 2 cut(s) 555, 1039
StyI CCWWGG 2 cut(s) 140, 365
TaaI ACNGT 4 cut(s) 15, 545, 1095, 1355
TaiI ACGT 2 cut(s) 131, 617
TaqI TCGA 8 cut(s) 20, 318, 600, 844, 862, 915, 938, 1036
TaqII GACCGA 1 cut(s) 228
TasI AATT 5 cut(s) 95, 226, 526, 699, 949
TatI WGTACW 1 cut(s) 1000
TauI GCSGC 1 cut(s) 1013
TfiI GAWTC 4 cut(s) 146, 156, 1103, 1291
Tru1I TTAA 5 cut(s) 270, 491, 872, 1016, 1262
Tru9I TTAA 5 cut(s) 270, 491, 872, 1016, 1262
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 209, 419
TseI GCWGC 3 cut(s) 38, 41, 44
Tsp45I GTSAC 2 cut(s) 209, 419
TspDTI ATGAA 6 cut(s) 505, 541, 1136, 1265, 1325, 1329
TspGWI ACGGA 2 cut(s) 70, 1002
TspRI CASTG 1 cut(s) 460
Van91I CCANNNNNTGG 1 cut(s) 991
VpaK11BI GGWCC 3 cut(s) 265, 295, 541
XapI RAATTY 1 cut(s) 949
XbaI TCTAGA 1 cut(s) 149
XceI RCATGY 1 cut(s) 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XmaJI CCTAGG 1 cut(s) 365
XspI CTAG 6 cut(s) 150, 366, 608, 644, 981, 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.