MD16G1065500.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001444
Physical Location & Seq
Forward (+)
4597935 .. 4598993
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1065500.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGCAGCGGACCCCACCGGCCCAACCTTGGTGGAAAAAATCAAGCCCTACCCACGAAAATTTGAGAATTCAACAATGTCCCGAATCAAAGAGCTCTCTCATCTCAGGCCCACCAGGCCCAAAGTCCCAGGTCCAACACACTGGCCCGGCCCTTGTATTCAGTGCCGGCGGGTACACCGGCAACATGTTCCATGATTTCAATGATGGGTTCATCCCAATCTTCATCATCGTCCATTCGATCCTCCACTATCAGGCGCCCATCCTTGTGATATCGGAAGCACGCGATCGGTGGGTTAGTAGGTATGCAGATTTACTGAAGATTTTTAGCAAGCACACGATCATAAACCTCGATAATGATACCGCCACCCATTGTTTTACTTCTGCCAGCGTGGGGCTTATATCTCACGGACTTGTCACCATAAACCCTACGTTGTTACACTACCCTAAAACGATGGTACATTTCCATGCGATCCTGGACAAAGCCTTCGGAAAACACCGCCGCGATTGCAAACCTTGGACTTCCAGGCCACGCCTCGTCTTACTCAGTCGAACCCTAGGCGTTGGCCGGGCGATCTTGACCCAAGACGATGCGAGGCGAGCAGCGGAGAAGGTTGGTTTTGAACTTGTGATTTTTGAGCCGACCTCTACAACTGACATGCACTTTGCGTACGATTTAATTAGTAAGAGCCACGCGATGGTGGGCGTGCATGGCGCGGCGCTTACGCACTTGTTGTTCATGAGGCCCGGCGCGGTGTTCATGCAGATTGTGCCTCTCGGCACCAACTGGCCGGCGAAGGTATGCTATGAGAGTCCGGCTAGGGCTATGGGGCTGCAGTACATTGACTATAGGATTGATGTAGAGGAGAGTAGCTTGGTGCACAAGTATGGGAAGGATGACATTGTGGTGAAAGATCCGGCTGCTAAAATTGGTTCCAATTGGGATATCTTGACGAAGATATATTTGAAGGAGCAAGATGTTAAGTTGGATTTGAATAGGTTTACTTGGTTTTTGAAGTCAGCTTATGGGAAGGCCAAGAGATTCATGGAGAAGGAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

353

Amino Acids

39.68

Weight (kDa)

9.34

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 142 - 252 1.3e-09 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 253, 776
AccB7I CCANNNNNTGG 1 cut(s) 694
AccII CGCG 5 cut(s) 282, 501, 692, 713, 749
AciI CCGC 8 cut(s) 7, 168, 360, 496, 499, 602, 713, 749
AclWI GGATC 3 cut(s) 232, 463, 905
AcoI YGGCCR 2 cut(s) 562, 785
AcsI RAATTY 2 cut(s) 58, 66
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 254
AfaI GTAC 4 cut(s) 173, 456, 668, 836
AfiI CCNNNNNNNGG 4 cut(s) 27, 250, 390, 694
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 6 cut(s) 71, 199, 620, 964, 991, 1012
AjnI CCWGG 4 cut(s) 112, 126, 471, 521
AluBI AGCT 3 cut(s) 93, 870, 1019
AluI AGCT 3 cut(s) 93, 870, 1019
Alw21I GWGCWC 2 cut(s) 95, 879
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 3 cut(s) 232, 463, 905
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 4 cut(s) 4, 599, 829, 917
ApoI RAATTY 2 cut(s) 58, 66
ArsI GACNNNNNNTTYG 4 cut(s) 467, 499, 644, 676
AspA2I CCTAGG 1 cut(s) 553
AspLEI GCGC 4 cut(s) 256, 713, 718, 749
AspS9I GGNCC 8 cut(s) 9, 19, 107, 116, 130, 143, 148, 741
AsuC2I CCSGG 3 cut(s) 146, 566, 744
AsuHPI GGTGA 2 cut(s) 406, 916
AvaII GGWCC 2 cut(s) 9, 130
AvrII CCTAGG 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 879
BanI GGYRCC 2 cut(s) 253, 776
BanII GRGCYC 1 cut(s) 95
Bbv12I GWGCWC 2 cut(s) 95, 879
BbvI GCAGC 4 cut(s) 16, 611, 816, 904
BccI CCATC 4 cut(s) 197, 266, 445, 688
BciT130I CCWGG 4 cut(s) 114, 128, 473, 523
BcnI CCSGG 3 cut(s) 146, 566, 744
BfaI CTAG 2 cut(s) 554, 816
BfmI CTRYAG 2 cut(s) 830, 844
BfoI RGCGCY 2 cut(s) 257, 719
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 6 cut(s) 5, 499, 600, 714, 830, 918
BlnI CCTAGG 1 cut(s) 553
BlsI GCNGC 6 cut(s) 6, 500, 601, 715, 831, 919
Bme1390I CCNGG 7 cut(s) 114, 128, 146, 473, 523, 566, 744
Bme18I GGWCC 2 cut(s) 9, 130
BmgT120I GGNCC 8 cut(s) 9, 19, 107, 116, 130, 143, 148, 741
BmiI GGNNCC 4 cut(s) 11, 255, 778, 931
BmrFI CCNGG 7 cut(s) 114, 128, 146, 473, 523, 566, 744
BmsI GCATC 1 cut(s) 577
BplI GAGNNNNNCTC 2 cut(s) 626, 658
BpuMI CCSGG 3 cut(s) 146, 566, 744
BsaHI GRCGYC 1 cut(s) 254
BsaJI CCNNGG 4 cut(s) 26, 126, 512, 553
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 250, 390, 694
Bse118I RCCGGY 4 cut(s) 16, 164, 176, 787
Bse1I ACTGG 2 cut(s) 145, 788
BseBI CCWGG 4 cut(s) 114, 128, 473, 523
BseDI CCNNGG 4 cut(s) 26, 126, 512, 553
BseGI GGATG 3 cut(s) 210, 258, 898
BseLI CCNNNNNNNGG 4 cut(s) 27, 250, 390, 694
BseMII CTCAG 2 cut(s) 117, 556
BseNI ACTGG 2 cut(s) 145, 788
BseRI GAGGAG 1 cut(s) 875
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 4 cut(s) 16, 611, 816, 904
Bsh1236I CGCG 5 cut(s) 282, 501, 692, 713, 749
Bsh1285I CGRYCG 1 cut(s) 286
BshNI GGYRCC 2 cut(s) 253, 776
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 2 cut(s) 95, 879
BsiSI CCGG 9 cut(s) 17, 146, 165, 177, 565, 744, 788, 812, 914
BsiWI CGTACG 1 cut(s) 666
BslFI GGGAC 2 cut(s) 63, 109
BslI CCNNNNNNNGG 4 cut(s) 27, 250, 390, 694
BsmFI GGGAC 2 cut(s) 63, 109
Bsp1286I GDGCHC 2 cut(s) 95, 879
Bsp143I GATC 6 cut(s) 237, 283, 336, 468, 570, 910
BspACI CCGC 8 cut(s) 7, 168, 360, 496, 499, 602, 713, 749
BspCNI CTCAG 2 cut(s) 116, 555
BspFNI CGCG 5 cut(s) 282, 501, 692, 713, 749
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 4 cut(s) 11, 255, 778, 931
BspMAI CTGCAG 1 cut(s) 834
BspPI GGATC 3 cut(s) 232, 463, 905
BspT107I GGYRCC 2 cut(s) 253, 776
BsrFI RCCGGY 4 cut(s) 16, 164, 176, 787
BsrI ACTGG 2 cut(s) 145, 788
BssAI RCCGGY 4 cut(s) 16, 164, 176, 787
BssECI CCNNGG 4 cut(s) 26, 126, 512, 553
BssMI GATC 6 cut(s) 237, 283, 336, 468, 570, 910
BssNI GRCGYC 1 cut(s) 254
BssT1I CCWWGG 3 cut(s) 26, 512, 553
Bst2UI CCWGG 4 cut(s) 114, 128, 473, 523
BstACI GRCGYC 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 766
BstC8I GCNNGC 7 cut(s) 166, 280, 329, 385, 597, 704, 789
BstDEI CTNAG 2 cut(s) 103, 542
BstF5I GGATG 3 cut(s) 210, 258, 898
BstFNI CGCG 5 cut(s) 282, 501, 692, 713, 749
BstH2I RGCGCY 2 cut(s) 257, 719
BstHHI GCGC 4 cut(s) 256, 713, 718, 749
BstKTI GATC 6 cut(s) 240, 286, 339, 471, 573, 913
BstMBI GATC 6 cut(s) 237, 283, 336, 468, 570, 910
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 5 cut(s) 114, 504, 596, 708, 766
BstNI CCWGG 4 cut(s) 114, 128, 473, 523
BstNSI RCATGY 2 cut(s) 187, 658
BstSCI CCNGG 7 cut(s) 112, 126, 144, 471, 521, 564, 742
BstSFI CTRYAG 2 cut(s) 830, 844
BstSLI GKGCMC 1 cut(s) 879
BstUI CGCG 5 cut(s) 282, 501, 692, 713, 749
BstV1I GCAGC 4 cut(s) 16, 611, 816, 904
BstX2I RGATCY 1 cut(s) 910
BstXI CCANNNNNNTGG 1 cut(s) 140
BstYI RGATCY 1 cut(s) 910
BtgZI GCGATG 1 cut(s) 707
BtsCI GGATG 3 cut(s) 210, 258, 898
BtsIMutI CAGTG 2 cut(s) 138, 166
Cac8I GCNNGC 7 cut(s) 166, 280, 329, 385, 597, 704, 789
CciI TCATGA 1 cut(s) 735
CfoI GCGC 4 cut(s) 256, 713, 718, 749
Cfr10I RCCGGY 4 cut(s) 16, 164, 176, 787
Cfr13I GGNCC 8 cut(s) 9, 19, 107, 116, 130, 143, 148, 741
Csp6I GTAC 4 cut(s) 172, 455, 667, 835
CspCI CAANNNNNGTGG 2 cut(s) 11, 46
CviAII CATG 8 cut(s) 184, 191, 464, 655, 707, 736, 757, 1042
CviQI GTAC 4 cut(s) 172, 455, 667, 835
DdeI CTNAG 2 cut(s) 103, 542
DinI GGCGCC 1 cut(s) 255
DpnI GATC 6 cut(s) 239, 285, 338, 470, 572, 912
DpnII GATC 6 cut(s) 237, 283, 336, 468, 570, 910
EaeI YGGCCR 2 cut(s) 562, 785
Ecl136II GAGCTC 1 cut(s) 93
Eco130I CCWWGG 3 cut(s) 26, 512, 553
Eco24I GRGCYC 1 cut(s) 95
Eco32I GATATC 2 cut(s) 270, 943
Eco47I GGWCC 2 cut(s) 9, 130
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 93
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 4 cut(s) 112, 126, 471, 521
EcoRV GATATC 2 cut(s) 270, 943
EcoT14I CCWWGG 3 cut(s) 26, 512, 553
EcoT38I GRGCYC 1 cut(s) 95
EgeI GGCGCC 1 cut(s) 255
EheI GGCGCC 1 cut(s) 255
ErhI CCWWGG 3 cut(s) 26, 512, 553
FaeI CATG 8 cut(s) 187, 194, 467, 658, 710, 739, 760, 1045
FaqI GGGAC 2 cut(s) 63, 109
FatI CATG 8 cut(s) 183, 190, 463, 654, 706, 735, 756, 1041
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 6 cut(s) 5, 499, 600, 714, 830, 918
FokI GGATG 3 cut(s) 197, 245, 905
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 6 cut(s) 5, 499, 600, 714, 830, 918
FspBI CTAG 2 cut(s) 554, 816
GlaI GCGC 4 cut(s) 255, 712, 717, 748
GluI GCNGC 6 cut(s) 5, 499, 600, 714, 830, 918
HaeII RGCGCY 2 cut(s) 257, 719
HapII CCGG 9 cut(s) 17, 146, 165, 177, 565, 744, 788, 812, 914
HhaI GCGC 4 cut(s) 256, 713, 718, 749
Hin1I GRCGYC 1 cut(s) 254
Hin1II CATG 8 cut(s) 187, 194, 467, 658, 710, 739, 760, 1045
Hin6I GCGC 4 cut(s) 254, 711, 716, 747
HinP1I GCGC 4 cut(s) 254, 711, 716, 747
HinfI GANTC 3 cut(s) 83, 808, 1038
HpaII CCGG 9 cut(s) 17, 146, 165, 177, 565, 744, 788, 812, 914
HphI GGTGA 2 cut(s) 406, 916
Hpy166II GTNNAC 3 cut(s) 174, 877, 999
Hpy188I TCNGA 2 cut(s) 274, 488
Hpy188III TCNNGA 4 cut(s) 80, 574, 736, 946
Hpy8I GTNNAC 3 cut(s) 174, 877, 999
HpyAV CCTTC 8 cut(s) 493, 601, 787, 883, 958, 1021, 1042, 1046
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 8 cut(s) 4, 305, 507, 658, 706, 760, 832, 877
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 504, 596, 708, 766
HpyF3I CTNAG 2 cut(s) 103, 542
HpySE526I ACGT 1 cut(s) 428
Hsp92I GRCGYC 1 cut(s) 254
Hsp92II CATG 8 cut(s) 187, 194, 467, 658, 710, 739, 760, 1045
HspAI GCGC 4 cut(s) 254, 711, 716, 747
KasI GGCGCC 1 cut(s) 253
KroI GCCGGC 2 cut(s) 164, 787
KroNI GCCGGC 2 cut(s) 166, 789
Kzo9I GATC 6 cut(s) 237, 283, 336, 468, 570, 910
LmnI GCTCC 1 cut(s) 967
Lsp1109I GCAGC 4 cut(s) 16, 611, 816, 904
LweI GCATC 1 cut(s) 577
MaeI CTAG 2 cut(s) 554, 816
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 412, 432
MalI GATC 6 cut(s) 239, 285, 338, 470, 572, 912
MboI GATC 6 cut(s) 237, 283, 336, 468, 570, 910
MboII GAAGA 3 cut(s) 211, 328, 964
MfeI CAATTG 1 cut(s) 934
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 2 cut(s) 95, 879
MluCI AATT 5 cut(s) 58, 66, 675, 924, 934
Mly113I GGCGCC 1 cut(s) 254
MlyI GAGTC 1 cut(s) 817
MmeI TCCRAC 2 cut(s) 157, 963
MnlI CCTC 8 cut(s) 251, 356, 542, 585, 652, 732, 780, 853
MroNI GCCGGC 2 cut(s) 164, 787
MseI TTAA 2 cut(s) 674, 978
MslI CAYNNNNRTG 4 cut(s) 263, 462, 882, 902
MspA1I CMGCKG 2 cut(s) 7, 602
MspI CCGG 9 cut(s) 17, 146, 165, 177, 565, 744, 788, 812, 914
MspR9I CCNGG 7 cut(s) 114, 128, 146, 473, 523, 566, 744
MteI GCGCNGCGC 1 cut(s) 714
MunI CAATTG 1 cut(s) 934
MvaI CCWGG 4 cut(s) 114, 128, 473, 523
MvnI CGCG 5 cut(s) 282, 501, 692, 713, 749
MwoI GCNNNNNNNGC 5 cut(s) 114, 504, 596, 708, 766
NaeI GCCGGC 2 cut(s) 166, 789
NarI GGCGCC 1 cut(s) 254
NciI CCSGG 3 cut(s) 146, 566, 744
NdeII GATC 6 cut(s) 237, 283, 336, 468, 570, 910
NgoMIV GCCGGC 2 cut(s) 164, 787
NlaIII CATG 8 cut(s) 187, 194, 467, 658, 710, 739, 760, 1045
NlaIV GGNNCC 4 cut(s) 11, 255, 778, 931
NmeAIII GCCGAG 1 cut(s) 753
NmuCI GTSAC 1 cut(s) 412
NspI RCATGY 2 cut(s) 187, 658
PagI TCATGA 1 cut(s) 735
PciI ACATGT 1 cut(s) 183
PdiI GCCGGC 2 cut(s) 166, 789
PfeI GAWTC 2 cut(s) 83, 1038
Pfl23II CGTACG 1 cut(s) 666
PflMI CCANNNNNTGG 1 cut(s) 694
PfoI TCCNGGA 1 cut(s) 471
PkrI GCNGC 6 cut(s) 6, 500, 601, 715, 831, 919
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 1 cut(s) 816
PluTI GGCGCC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 816
PscI ACATGT 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 95
Psp6I CCWGG 4 cut(s) 112, 126, 471, 521
PspGI CCWGG 4 cut(s) 112, 126, 471, 521
PspLI CGTACG 1 cut(s) 666
PspN4I GGNNCC 4 cut(s) 11, 255, 778, 931
PspPI GGNCC 8 cut(s) 9, 19, 107, 116, 130, 143, 148, 741
PstI CTGCAG 1 cut(s) 834
PsuI RGATCY 1 cut(s) 910
PvuI CGATCG 1 cut(s) 286
RsaI GTAC 4 cut(s) 173, 456, 668, 836
RsaNI GTAC 4 cut(s) 172, 455, 667, 835
RseI CAYNNNNRTG 4 cut(s) 263, 462, 882, 902
SacI GAGCTC 1 cut(s) 95
SaqAI TTAA 2 cut(s) 674, 978
SatI GCNGC 6 cut(s) 5, 499, 600, 714, 830, 918
Sau3AI GATC 6 cut(s) 237, 283, 336, 468, 570, 910
Sau96I GGNCC 8 cut(s) 9, 19, 107, 116, 130, 143, 148, 741
SchI GAGTC 1 cut(s) 817
ScrFI CCNGG 7 cut(s) 114, 128, 146, 473, 523, 566, 744
SduI GDGCHC 2 cut(s) 95, 879
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 2 cut(s) 830, 844
SfiI GGCCNNNNNGGCC 1 cut(s) 114
SfoI GGCGCC 1 cut(s) 255
SinI GGWCC 2 cut(s) 9, 130
SmiMI CAYNNNNRTG 4 cut(s) 263, 462, 882, 902
Sse9I AATT 5 cut(s) 58, 66, 675, 924, 934
SsiI CCGC 8 cut(s) 7, 168, 360, 496, 499, 602, 713, 749
SspDI GGCGCC 1 cut(s) 253
SspMI CTAG 2 cut(s) 554, 816
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 7 cut(s) 112, 126, 144, 471, 521, 564, 742
StyI CCWWGG 3 cut(s) 26, 512, 553
TaiI ACGT 1 cut(s) 431
TaqI TCGA 3 cut(s) 236, 348, 547
TasI AATT 5 cut(s) 58, 66, 675, 924, 934
TatI WGTACW 1 cut(s) 834
TauI GCSGC 2 cut(s) 501, 716
TfiI GAWTC 2 cut(s) 83, 1038
Tru1I TTAA 2 cut(s) 674, 978
Tru9I TTAA 2 cut(s) 674, 978
TscAI CASTG 2 cut(s) 145, 166
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 4 cut(s) 4, 599, 829, 917
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 5 cut(s) 199, 211, 724, 745, 1030
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 2 cut(s) 145, 166
Van91I CCANNNNNTGG 1 cut(s) 694
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 9, 130
XapI RAATTY 2 cut(s) 58, 66
XceI RCATGY 2 cut(s) 187, 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XmaJI CCTAGG 1 cut(s) 553
XspI CTAG 2 cut(s) 554, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.