RchiOBHm_Chr4g0438931

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001444
Physical Location & Seq
Forward (+)
61454373 .. 61455635
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40701

Sequence Viewer

Length: 1263 bp
ATGTGTTTTTACATTTATCAAATTTTGATTTCAGAAATAACACGTGAGATATTGGAAATTCATGAACAAATCCATCACCCTGCACAGAAAAGTCCGATCAGTTGCGACCGTACCTGTAAACATTACGATATCTGCTCGATCAACGGCCCAACAGGCTTGGATCCAACCACATCCACATTCTTCACTATGGACTCATCCCAACTTGCACCCCTGGAAGTGGAAAGAATCCGACCTTACCCTCGAAAATTCGAAGATTTCATAATGGCCCAGATCAAGGACCTCACACTCGCTGTTGGCCCACGAAGCCCACCGTGTAAAGTCCAACACAATGCTCCGGCCCTAGTATTCGCCGCCGGTGGATACACCGGAAACTTTTGGCATGACTTCAATGATGGATTTATCCCGCTCTTTATCACCGCCAACACACTCTTCCCAGATCAAGACTTCGTGATCGTGGTTTCCCAAGCTCCAAAGTGGTGGATTTATAAGTATGCCGATTTACTACGCATATTCACCAAGCACCCTATTATTACCATGGGAAATGATGCCACCACCCATTGTTTTCCTTCTGCCAGTCTAGGCCTTATATCACATGGTTTCATGACTATAAACCAAACACTAATACCTCGTGCAAAAACGTTTCTGCATTTTCGTGGGCTCCTAGACAACGCATATAAGCGAAGGTCCCAACCTCAAATTCTCTCCTCTGAGCAGACAAAACCCCGGCCACGCCTTGTTTTGGCGAGCCGGAATCATGCAACTGGACGCAGGATCGTTAACCAGGTTCAAGTAGTTCGTTTGGTAAAGAAAGTGGGGTTTGATGTGATTGTGTTTGAGCCCAAGGCCAATAACTCATTGCATGAGTCTTATGCGTTACTTAATTCAAGCCATGCTTTTGTTGGGGTACATGGCGCTGCACTTACACACTCATTGTTCCTCCGGCCGGGTTCAGTGTTCGTACAGGTGGTGCCGATTGGGGTGGACTGGGCGGCGAATGCGTGTTTCGGGAGAGTGGCGAAGGGGCTGAGGTTGGAGTATTTTGAGTACAAGATCGGAGTGAAAGAGAGTAGCTTGGTGGACAAGTATGGTAATGATAGTTTGGTGGTAAAAGATCCATTTGGTCTTCAGAAAATGGGGTGGCGTGCTGAGGTTATGGACATTTACTTGAAGGAGCAGAATGTGAAGCTGGATTTGGTTAGGTTCAAGGGGTATTTGAAGCAAGCGTTTAAGAAGGCTAAGAAATTTATGGACAACAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

420

Amino Acids

47.58

Weight (kDa)

9.37

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 126 - 320 2e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 967
AccBSI CCGCTC 1 cut(s) 406
AciI CCGC 4 cut(s) 351, 404, 417, 989
AclI AACGTT 1 cut(s) 638
AclWI GGATC 4 cut(s) 155, 168, 779, 1106
AcoI YGGCCR 2 cut(s) 725, 941
AcsI RAATTY 5 cut(s) 21, 57, 245, 696, 1241
AcuI CTGAAG 1 cut(s) 1109
AcvI CACGTG 1 cut(s) 44
AfaI GTAC 4 cut(s) 112, 906, 960, 1046
AfiI CCNNNNNNNGG 4 cut(s) 217, 274, 739, 943
AflIII ACRYGT 1 cut(s) 41
AgsI TTSAA 6 cut(s) 388, 788, 885, 1168, 1204, 1216
AjnI CCWGG 2 cut(s) 210, 780
AjuI GAANNNNNNNTTGG 6 cut(s) 413, 445, 681, 713, 1175, 1207
AluBI AGCT 3 cut(s) 467, 1071, 1186
AluI AGCT 3 cut(s) 467, 1071, 1186
AlwI GGATC 4 cut(s) 155, 168, 779, 1106
AoxI GGCC 8 cut(s) 145, 264, 295, 336, 580, 725, 843, 941
ApeKI GCWGC 1 cut(s) 914
ApoI RAATTY 5 cut(s) 21, 57, 245, 696, 1241
AspLEI GCGC 1 cut(s) 914
AspS9I GGNCC 6 cut(s) 146, 265, 277, 296, 337, 684
AsuC2I CCSGG 2 cut(s) 724, 945
AsuHPI GGTGA 3 cut(s) 68, 406, 505
AsuII TTCGAA 1 cut(s) 249
AvaII GGWCC 2 cut(s) 277, 684
BamHI GGATCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 967
BanII GRGCYC 2 cut(s) 660, 840
BauI CACGAG 1 cut(s) 627
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 1115
BbvCI CCTCAGC 2 cut(s) 1025, 1146
BbvI GCAGC 1 cut(s) 901
BccI CCATC 2 cut(s) 81, 386
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 2 cut(s) 212, 782
BciVI GTATCC 1 cut(s) 353
BcnI CCSGG 2 cut(s) 724, 945
BfaI CTAG 3 cut(s) 341, 578, 662
BfoI RGCGCY 1 cut(s) 915
BfuI GTATCC 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 153
BisI GCNGC 3 cut(s) 351, 915, 990
BlsI GCNGC 3 cut(s) 352, 916, 991
Bme1390I CCNGG 4 cut(s) 212, 724, 782, 945
Bme18I GGWCC 2 cut(s) 277, 684
BmgT120I GGNCC 6 cut(s) 146, 265, 277, 296, 337, 684
BmiI GGNNCC 4 cut(s) 162, 659, 686, 969
BmrFI CCNGG 4 cut(s) 212, 724, 782, 945
BmrI ACTGGG 1 cut(s) 994
BmsI GCATC 1 cut(s) 535
BmuI ACTGGG 1 cut(s) 994
BpiI GAAGAC 1 cut(s) 1115
Bpu10I CCTNAGC 2 cut(s) 1025, 1146
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 724, 945
BsaAI YACGTR 1 cut(s) 44
BsaJI CCNNGG 4 cut(s) 210, 534, 722, 840
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 274, 739, 943
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 573, 766, 989
Bse3DI GCAATG 1 cut(s) 854
BseBI CCWGG 2 cut(s) 212, 782
BseDI CCNNGG 4 cut(s) 210, 534, 722, 840
BseGI GGATG 2 cut(s) 170, 194
BseLI CCNNNNNNNGG 4 cut(s) 217, 274, 739, 943
BseMI GCAATG 1 cut(s) 854
BseMII CTCAG 3 cut(s) 699, 1016, 1137
BseNI ACTGG 3 cut(s) 573, 766, 989
BseRI GAGGAG 1 cut(s) 694
BseX3I CGGCCG 1 cut(s) 941
BseXI GCAGC 1 cut(s) 901
BsgI GTGCAG 2 cut(s) 66, 900
Bsh1285I CGRYCG 2 cut(s) 109, 944
BshFI GGCC 8 cut(s) 147, 266, 297, 338, 582, 727, 845, 943
BshNI GGYRCC 1 cut(s) 967
BsiEI CGRYCG 2 cut(s) 109, 944
BsiSI CCGG 7 cut(s) 335, 354, 366, 724, 748, 940, 944
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 4 cut(s) 217, 274, 739, 943
BsmFI GGGAC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 8 cut(s) 147, 266, 297, 338, 582, 727, 845, 943
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 660, 840
Bsp143I GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 4 cut(s) 351, 404, 417, 989
BspANI GGCC 8 cut(s) 147, 266, 297, 338, 582, 727, 845, 943
BspCNI CTCAG 3 cut(s) 700, 1017, 1138
BspHI TCATGA 2 cut(s) 61, 600
BspLI GGNNCC 4 cut(s) 162, 659, 686, 969
BspPI GGATC 4 cut(s) 155, 168, 779, 1106
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 967
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 854
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 3 cut(s) 573, 766, 989
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 4 cut(s) 210, 534, 722, 840
BssMI GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
BssSI CACGAG 1 cut(s) 627
BssT1I CCWWGG 2 cut(s) 534, 840
Bst2BI CACGAG 1 cut(s) 627
Bst2UI CCWGG 2 cut(s) 212, 782
Bst4CI ACNGT 2 cut(s) 110, 312
Bst6I CTCTTC 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 745, 1143, 1221
BstDEI CTNAG 4 cut(s) 708, 1025, 1146, 1236
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 170, 194
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 9 cut(s) 99, 141, 163, 273, 439, 453, 774, 1053, 1114
BstMBI GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
BstMCI CGRYCG 2 cut(s) 109, 944
BstMWI GCNNNNNNNGC 3 cut(s) 153, 303, 995
BstNI CCWGG 2 cut(s) 212, 782
BstSCI CCNGG 4 cut(s) 210, 722, 780, 943
BstV1I GCAGC 1 cut(s) 901
BstV2I GAAGAC 1 cut(s) 1115
BstX2I RGATCY 2 cut(s) 160, 1111
BstXI CCANNNNNNTGG 1 cut(s) 477
BstYI RGATCY 2 cut(s) 160, 1111
BstZI CGGCCG 1 cut(s) 941
BsuI GTATCC 1 cut(s) 353
BsuRI GGCC 8 cut(s) 147, 266, 297, 338, 582, 727, 845, 943
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 170, 194
BtsIMutI CAGTG 1 cut(s) 957
Cac8I GCNNGC 3 cut(s) 745, 1143, 1221
CciI TCATGA 2 cut(s) 61, 600
CfoI GCGC 1 cut(s) 914
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 6 cut(s) 146, 265, 277, 296, 337, 684
CseI GACGC 1 cut(s) 774
CsiI ACCWGGT 1 cut(s) 780
Csp6I GTAC 4 cut(s) 111, 905, 959, 1045
CviAII CATG 9 cut(s) 62, 380, 535, 593, 601, 755, 860, 890, 908
CviQI GTAC 4 cut(s) 111, 905, 959, 1045
DdeI CTNAG 4 cut(s) 708, 1025, 1146, 1236
DpnI GATC 9 cut(s) 98, 140, 162, 272, 438, 452, 773, 1052, 1113
DpnII GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
EaeI YGGCCR 2 cut(s) 725, 941
EagI CGGCCG 1 cut(s) 941
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
EclXI CGGCCG 1 cut(s) 941
Eco130I CCWWGG 2 cut(s) 534, 840
Eco147I AGGCCT 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 660, 840
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 277, 684
Eco52I CGGCCG 1 cut(s) 941
Eco57I CTGAAG 1 cut(s) 1109
Eco72I CACGTG 1 cut(s) 44
EcoO109I RGGNCCY 2 cut(s) 277, 684
EcoRII CCWGG 2 cut(s) 210, 780
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 2 cut(s) 534, 840
EcoT38I GRGCYC 2 cut(s) 660, 840
ErhI CCWWGG 2 cut(s) 534, 840
FaeI CATG 9 cut(s) 65, 383, 538, 596, 604, 758, 863, 893, 911
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FaqI GGGAC 1 cut(s) 670
FatI CATG 9 cut(s) 61, 379, 534, 592, 600, 754, 859, 889, 907
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 3 cut(s) 351, 915, 990
FokI GGATG 2 cut(s) 157, 181
FriOI GRGCYC 2 cut(s) 660, 840
Fsp4HI GCNGC 3 cut(s) 351, 915, 990
FspBI CTAG 3 cut(s) 341, 578, 662
GlaI GCGC 1 cut(s) 913
GluI GCNGC 3 cut(s) 351, 915, 990
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 8 cut(s) 147, 266, 297, 338, 582, 727, 845, 943
HapII CCGG 7 cut(s) 335, 354, 366, 724, 748, 940, 944
HgaI GACGC 1 cut(s) 774
HhaI GCGC 1 cut(s) 914
Hin1II CATG 9 cut(s) 65, 383, 538, 596, 604, 758, 863, 893, 911
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 4 cut(s) 191, 225, 751, 863
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 7 cut(s) 335, 354, 366, 724, 748, 940, 944
HphI GGTGA 3 cut(s) 68, 406, 505
Hpy166II GTNNAC 4 cut(s) 119, 778, 982, 1078
Hpy188I TCNGA 6 cut(s) 34, 96, 230, 709, 1055, 1128
Hpy188III TCNNGA 5 cut(s) 62, 440, 448, 601, 1006
Hpy8I GTNNAC 4 cut(s) 119, 778, 982, 1078
HpyAV CCTTC 5 cut(s) 576, 675, 1012, 1162, 1225
HpyCH4III ACNGT 2 cut(s) 110, 312
HpyCH4IV ACGT 2 cut(s) 43, 638
HpyCH4V TGCA 7 cut(s) 83, 206, 632, 646, 758, 859, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 303, 995
HpyF3I CTNAG 4 cut(s) 708, 1025, 1146, 1236
HpySE526I ACGT 2 cut(s) 43, 638
Hsp92II CATG 9 cut(s) 65, 383, 538, 596, 604, 758, 863, 893, 911
HspAI GCGC 1 cut(s) 912
KspAI GTTAAC 1 cut(s) 778
Kzo9I GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
LmnI GCTCC 4 cut(s) 337, 472, 663, 1171
Lsp1109I GCAGC 1 cut(s) 901
LweI GCATC 1 cut(s) 535
MabI ACCWGGT 1 cut(s) 780
MaeI CTAG 3 cut(s) 341, 578, 662
MaeII ACGT 2 cut(s) 43, 638
MaeIII GTNAC 1 cut(s) 873
MalI GATC 9 cut(s) 98, 140, 162, 272, 438, 452, 773, 1052, 1113
MbiI CCGCTC 1 cut(s) 406
MboI GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
MboII GAAGA 4 cut(s) 172, 263, 421, 1115
MflI RGATCY 2 cut(s) 160, 1111
MhlI GDGCHC 2 cut(s) 660, 840
MluCI AATT 6 cut(s) 21, 57, 245, 696, 880, 1241
MlyI GAGTC 2 cut(s) 185, 872
MmeI TCCRAC 4 cut(s) 188, 253, 346, 1011
MnlI CCTC 8 cut(s) 249, 290, 636, 702, 715, 947, 1020, 1141
MseI TTAA 3 cut(s) 777, 879, 1227
MslI CAYNNNNRTG 1 cut(s) 651
MspI CCGG 7 cut(s) 335, 354, 366, 724, 748, 940, 944
MspR9I CCNGG 4 cut(s) 212, 724, 782, 945
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 2 cut(s) 212, 782
MwoI GCNNNNNNNGC 3 cut(s) 153, 303, 995
NciI CCSGG 2 cut(s) 724, 945
NcoI CCATGG 1 cut(s) 534
NdeII GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
NlaIII CATG 9 cut(s) 65, 383, 538, 596, 604, 758, 863, 893, 911
NlaIV GGNNCC 4 cut(s) 162, 659, 686, 969
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 2 cut(s) 61, 600
PceI AGGCCT 1 cut(s) 582
PctI GAATGC 1 cut(s) 1000
PfeI GAWTC 2 cut(s) 225, 751
PkrI GCNGC 3 cut(s) 352, 916, 991
PleI GAGTC 2 cut(s) 185, 871
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 2 cut(s) 185, 871
Ppu21I YACGTR 1 cut(s) 44
PpuMI RGGWCCY 2 cut(s) 277, 684
PsiI TTATAA 1 cut(s) 486
Psp1406I AACGTT 1 cut(s) 638
Psp5II RGGWCCY 2 cut(s) 277, 684
Psp6I CCWGG 2 cut(s) 210, 780
PspCI CACGTG 1 cut(s) 44
PspGI CCWGG 2 cut(s) 210, 780
PspN4I GGNNCC 4 cut(s) 162, 659, 686, 969
PspPI GGNCC 6 cut(s) 146, 265, 277, 296, 337, 684
PspPPI RGGWCCY 2 cut(s) 277, 684
PsuI RGATCY 2 cut(s) 160, 1111
RsaI GTAC 4 cut(s) 112, 906, 960, 1046
RsaNI GTAC 4 cut(s) 111, 905, 959, 1045
RseI CAYNNNNRTG 1 cut(s) 651
SaqAI TTAA 3 cut(s) 777, 879, 1227
SatI GCNGC 3 cut(s) 351, 915, 990
Sau3AI GATC 9 cut(s) 96, 138, 160, 270, 436, 450, 771, 1050, 1111
Sau96I GGNCC 6 cut(s) 146, 265, 277, 296, 337, 684
SchI GAGTC 2 cut(s) 185, 872
ScrFI CCNGG 4 cut(s) 212, 724, 782, 945
SduI GDGCHC 2 cut(s) 660, 840
SexAI ACCWGGT 1 cut(s) 780
SfaNI GCATC 1 cut(s) 535
SfuI TTCGAA 1 cut(s) 249
SgrAI CRCCGGYG 1 cut(s) 353
SinI GGWCC 2 cut(s) 277, 684
SmiMI CAYNNNNRTG 1 cut(s) 651
Sse9I AATT 6 cut(s) 21, 57, 245, 696, 880, 1241
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 4 cut(s) 351, 404, 417, 989
SspMI CTAG 3 cut(s) 341, 578, 662
StuI AGGCCT 1 cut(s) 582
StyD4I CCNGG 4 cut(s) 210, 722, 780, 943
StyI CCWWGG 2 cut(s) 534, 840
TaaI ACNGT 2 cut(s) 110, 312
TaiI ACGT 2 cut(s) 46, 641
TaqI TCGA 3 cut(s) 137, 241, 249
TasI AATT 6 cut(s) 21, 57, 245, 696, 880, 1241
TatI WGTACW 1 cut(s) 1044
TauI GCSGC 2 cut(s) 353, 992
TfiI GAWTC 2 cut(s) 225, 751
Tru1I TTAA 3 cut(s) 777, 879, 1227
Tru9I TTAA 3 cut(s) 777, 879, 1227
TscAI CASTG 1 cut(s) 957
TseI GCWGC 1 cut(s) 914
TspDTI ATGAA 4 cut(s) 50, 78, 247, 589
TspRI CASTG 1 cut(s) 957
VpaK11BI GGWCC 2 cut(s) 277, 684
XapI RAATTY 5 cut(s) 21, 57, 245, 696, 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XspI CTAG 3 cut(s) 341, 578, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.