Rh4AG365100

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001444
Physical Location & Seq
Forward (+)
67326896 .. 67328777
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG365100.1

Sequence Viewer

Length: 1359 bp
ATGAGGAAGATGAAGAAGAGGGTTTTGGTAGGATTGGCTTCTTTGTGCTTTTGTTTTATTGTATTCCATATCAGTGTCTCATTAACCTCCAGATTTCCTCATAGTGCAGCATTTTCAGTAGAGACTCGAGAAATAACACGTGAGATATTGGAAATTCATGAACAAATCCATCACCCTGCACAGAAAAGTCCGATCAGTTGCGACCGTACCTGTAAACATTACGATATCTGCTCGATCAACGGCCCAACAGGCTTGGATCCAACCACATCCACATTCTTCACTATGGACTCATCCCAACTTGCACCCCTGGAAGTGGAAAGAATCCGACCTTACCCTCGAAAATTCGAAGATTTCATAATGGCCCAGATCAAGGACCTCACACTCGCTGTTGGCCCACGAAGCCCACCGTGTAAAGTCCAACACAATGCTCCGGCCCTAGTATTCGCCGCCGGTGGATACACCGGAAACTTTTGGCATGACTTCAATGATGGATTTATCCCGCTCTTTATCACCGCCAACACACTCTTCCCAGATCAAGACTTCGTGATCGTGGTTTCCCAAGCTCCAAAGTGGTGGATTTATAAGTATGCCGATTTACTACGCATATTCACCAAGCACCCTATTATTACCATGGGAAATGATGCCACCACCCATTGTTTTCCTTCTGCCAGTCTAGGCCTTATATCACATGGTTTCATGACTATAAACCAAACACTAATACCTCGTGCAAAAACGTTTCTGCATTTTCGTGGGCTCCTAGACAACGCATATAAGCGAAGGTCCCAACCTCAAATTCTCTCCTCTGAGCAGACAAAACCCCGGCCACGCCTTGTTTTGGCGAGCCGGAATCATGCAACTGGACGCAGGATCGTTAACCAGGTTCAAGTAGTTCGTTTGGTAAAGAAAGTGGGGTTTGATGTGATTGTGTTTGAGCCCAAGGCCAATAACTCATTGCATGAGTCTTATGCGTTACTTAATTCAAGCCATGCTTTTGTTGGGGTACATGGCGCTGCACTTACACACTCATTGTTCCTCCGGCCGGGTTCAGTGTTCGTACAGGTGGTGCCGATTGGGGTGGACTGGGCGGCGAATGCGTGTTTCGGGAGAGTGGCGAAGGGGCTGAGGTTGGAGTATTTTGAGTACAAGATCGGAGTGAAAGAGAGTAGCTTGGTGGACAAGTATGGTAATGATAGTTTGGTGGTAAAAGATCCATTTGGTCTTCAGAAAATGGGGTGGCGTGCTGAGGTTATGGACATTTACTTGAAGGAGCAGAATGTGAAGCTGGATTTGGTTAGGTTCAAGGGGTATTTGAAGCAAGCGTTTAAGAAGGCTAAGAAATTTATGGACAACAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

452

Amino Acids

51.09

Weight (kDa)

9.61

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 158 - 352 2.4e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 1063
AccBSI CCGCTC 1 cut(s) 502
AciI CCGC 4 cut(s) 447, 500, 513, 1085
AclI AACGTT 1 cut(s) 734
AclWI GGATC 4 cut(s) 251, 264, 875, 1202
AcoI YGGCCR 2 cut(s) 821, 1037
AcsI RAATTY 4 cut(s) 153, 341, 792, 1337
AcuI CTGAAG 1 cut(s) 1205
AcvI CACGTG 1 cut(s) 140
AfaI GTAC 4 cut(s) 208, 1002, 1056, 1142
AfiI CCNNNNNNNGG 4 cut(s) 313, 370, 835, 1039
AflIII ACRYGT 1 cut(s) 137
AgsI TTSAA 6 cut(s) 484, 884, 981, 1264, 1300, 1312
AjnI CCWGG 2 cut(s) 306, 876
AjuI GAANNNNNNNTTGG 8 cut(s) 8, 40, 509, 541, 777, 809, 1271, 1303
AluBI AGCT 3 cut(s) 563, 1167, 1282
AluI AGCT 3 cut(s) 563, 1167, 1282
Alw26I GTCTC 2 cut(s) 82, 116
AlwI GGATC 4 cut(s) 251, 264, 875, 1202
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 8 cut(s) 241, 360, 391, 432, 676, 821, 939, 1037
ApeKI GCWGC 2 cut(s) 107, 1010
ApoI RAATTY 4 cut(s) 153, 341, 792, 1337
AspLEI GCGC 1 cut(s) 1010
AspS9I GGNCC 6 cut(s) 242, 361, 373, 392, 433, 780
AsuC2I CCSGG 2 cut(s) 820, 1041
AsuHPI GGTGA 3 cut(s) 164, 502, 601
AsuII TTCGAA 1 cut(s) 345
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 2 cut(s) 373, 780
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 1063
BanII GRGCYC 2 cut(s) 756, 936
BauI CACGAG 1 cut(s) 723
BbrPI CACGTG 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 1211
BbvCI CCTCAGC 2 cut(s) 1121, 1242
BbvI GCAGC 2 cut(s) 119, 997
BccI CCATC 2 cut(s) 177, 482
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 2 cut(s) 308, 878
BciVI GTATCC 1 cut(s) 449
BcnI CCSGG 2 cut(s) 820, 1041
BcoDI GTCTC 2 cut(s) 82, 116
BfaI CTAG 3 cut(s) 437, 674, 758
BfoI RGCGCY 1 cut(s) 1011
BfuI GTATCC 1 cut(s) 449
BglI GCCNNNNNGGC 1 cut(s) 249
BisI GCNGC 4 cut(s) 108, 447, 1011, 1086
BlsI GCNGC 4 cut(s) 109, 448, 1012, 1087
Bme1390I CCNGG 4 cut(s) 308, 820, 878, 1041
Bme18I GGWCC 2 cut(s) 373, 780
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 6 cut(s) 242, 361, 373, 392, 433, 780
BmiI GGNNCC 4 cut(s) 258, 755, 782, 1065
BmrFI CCNGG 4 cut(s) 308, 820, 878, 1041
BmrI ACTGGG 1 cut(s) 1090
BmsI GCATC 1 cut(s) 631
BmuI ACTGGG 1 cut(s) 1090
BpiI GAAGAC 1 cut(s) 1211
BpmI CTGGAG 1 cut(s) 73
Bpu10I CCTNAGC 2 cut(s) 1121, 1242
Bpu14I TTCGAA 1 cut(s) 345
BpuMI CCSGG 2 cut(s) 820, 1041
BsaAI YACGTR 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 306, 630, 818, 936
BsaWI WCCGGW 1 cut(s) 461
Bsc4I CCNNNNNNNGG 4 cut(s) 313, 370, 835, 1039
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 3 cut(s) 669, 862, 1085
Bse3DI GCAATG 1 cut(s) 950
BseBI CCWGG 2 cut(s) 308, 878
BseDI CCNNGG 4 cut(s) 306, 630, 818, 936
BseGI GGATG 2 cut(s) 266, 290
BseLI CCNNNNNNNGG 4 cut(s) 313, 370, 835, 1039
BseMI GCAATG 1 cut(s) 950
BseMII CTCAG 3 cut(s) 795, 1112, 1233
BseNI ACTGG 3 cut(s) 669, 862, 1085
BseRI GAGGAG 1 cut(s) 790
BseX3I CGGCCG 1 cut(s) 1037
BseXI GCAGC 2 cut(s) 119, 997
BsgI GTGCAG 3 cut(s) 126, 162, 996
Bsh1285I CGRYCG 2 cut(s) 205, 1040
BshFI GGCC 8 cut(s) 243, 362, 393, 434, 678, 823, 941, 1039
BshNI GGYRCC 1 cut(s) 1063
BsiEI CGRYCG 2 cut(s) 205, 1040
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 7 cut(s) 431, 450, 462, 820, 844, 1036, 1040
BslFI GGGAC 1 cut(s) 766
BslI CCNNNNNNNGG 4 cut(s) 313, 370, 835, 1039
BsmAI GTCTC 2 cut(s) 82, 116
BsmFI GGGAC 1 cut(s) 766
BsmI GAATGC 1 cut(s) 1096
BsnI GGCC 8 cut(s) 243, 362, 393, 434, 678, 823, 941, 1039
BsoBI CYCGRG 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 345
Bsp1286I GDGCHC 2 cut(s) 756, 936
Bsp143I GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 4 cut(s) 447, 500, 513, 1085
BspANI GGCC 8 cut(s) 243, 362, 393, 434, 678, 823, 941, 1039
BspCNI CTCAG 3 cut(s) 796, 1113, 1234
BspHI TCATGA 2 cut(s) 157, 696
BspLI GGNNCC 4 cut(s) 258, 755, 782, 1065
BspPI GGATC 4 cut(s) 251, 264, 875, 1202
BspT104I TTCGAA 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 1063
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 950
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 3 cut(s) 669, 862, 1085
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 4 cut(s) 306, 630, 818, 936
BssMI GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 2 cut(s) 630, 936
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 2 cut(s) 308, 878
Bst4CI ACNGT 2 cut(s) 206, 408
Bst6I CTCTTC 2 cut(s) 11, 530
BstBAI YACGTR 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 345
BstC8I GCNNGC 3 cut(s) 841, 1239, 1317
BstDEI CTNAG 4 cut(s) 804, 1121, 1242, 1332
BstDSI CCRYGG 1 cut(s) 630
BstF5I GGATG 2 cut(s) 266, 290
BstH2I RGCGCY 1 cut(s) 1011
BstHHI GCGC 1 cut(s) 1010
BstKTI GATC 9 cut(s) 195, 237, 259, 369, 535, 549, 870, 1149, 1210
BstMAI GTCTC 2 cut(s) 82, 116
BstMBI GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
BstMCI CGRYCG 2 cut(s) 205, 1040
BstMWI GCNNNNNNNGC 3 cut(s) 249, 399, 1091
BstNI CCWGG 2 cut(s) 308, 878
BstSCI CCNGG 4 cut(s) 306, 818, 876, 1039
BstV1I GCAGC 2 cut(s) 119, 997
BstV2I GAAGAC 1 cut(s) 1211
BstX2I RGATCY 2 cut(s) 256, 1207
BstXI CCANNNNNNTGG 1 cut(s) 573
BstYI RGATCY 2 cut(s) 256, 1207
BstZI CGGCCG 1 cut(s) 1037
BsuI GTATCC 1 cut(s) 449
BsuRI GGCC 8 cut(s) 243, 362, 393, 434, 678, 823, 941, 1039
BtgI CCRYGG 1 cut(s) 630
BtsCI GGATG 2 cut(s) 266, 290
BtsIMutI CAGTG 2 cut(s) 79, 1053
Cac8I GCNNGC 3 cut(s) 841, 1239, 1317
CciI TCATGA 2 cut(s) 157, 696
CfoI GCGC 1 cut(s) 1010
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 6 cut(s) 242, 361, 373, 392, 433, 780
CseI GACGC 1 cut(s) 870
CsiI ACCWGGT 1 cut(s) 876
Csp6I GTAC 4 cut(s) 207, 1001, 1055, 1141
CviAII CATG 9 cut(s) 158, 476, 631, 689, 697, 851, 956, 986, 1004
CviQI GTAC 4 cut(s) 207, 1001, 1055, 1141
DdeI CTNAG 4 cut(s) 804, 1121, 1242, 1332
DpnI GATC 9 cut(s) 194, 236, 258, 368, 534, 548, 869, 1148, 1209
DpnII GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
EaeI YGGCCR 2 cut(s) 821, 1037
EagI CGGCCG 1 cut(s) 1037
Eam1104I CTCTTC 2 cut(s) 11, 530
EarI CTCTTC 2 cut(s) 11, 530
EclXI CGGCCG 1 cut(s) 1037
Eco130I CCWWGG 2 cut(s) 630, 936
Eco147I AGGCCT 1 cut(s) 678
Eco24I GRGCYC 2 cut(s) 756, 936
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 2 cut(s) 373, 780
Eco52I CGGCCG 1 cut(s) 1037
Eco57I CTGAAG 1 cut(s) 1205
Eco72I CACGTG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 126
EcoO109I RGGNCCY 2 cut(s) 373, 780
EcoRII CCWGG 2 cut(s) 306, 876
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 630, 936
EcoT38I GRGCYC 2 cut(s) 756, 936
ErhI CCWWGG 2 cut(s) 630, 936
FaeI CATG 9 cut(s) 161, 479, 634, 692, 700, 854, 959, 989, 1007
FalI AAGNNNNNCTT 2 cut(s) 973, 1005
FaqI GGGAC 1 cut(s) 766
FatI CATG 9 cut(s) 157, 475, 630, 688, 696, 850, 955, 985, 1003
FauI CCCGC 1 cut(s) 507
Fnu4HI GCNGC 4 cut(s) 108, 447, 1011, 1086
FokI GGATG 2 cut(s) 253, 277
FriOI GRGCYC 2 cut(s) 756, 936
Fsp4HI GCNGC 4 cut(s) 108, 447, 1011, 1086
FspBI CTAG 3 cut(s) 437, 674, 758
GlaI GCGC 1 cut(s) 1009
GluI GCNGC 4 cut(s) 108, 447, 1011, 1086
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 1011
HaeIII GGCC 8 cut(s) 243, 362, 393, 434, 678, 823, 941, 1039
HapII CCGG 7 cut(s) 431, 450, 462, 820, 844, 1036, 1040
HgaI GACGC 1 cut(s) 870
HhaI GCGC 1 cut(s) 1010
Hin1II CATG 9 cut(s) 161, 479, 634, 692, 700, 854, 959, 989, 1007
Hin6I GCGC 1 cut(s) 1008
HinP1I GCGC 1 cut(s) 1008
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HinfI GANTC 5 cut(s) 124, 287, 321, 847, 959
HpaI GTTAAC 1 cut(s) 874
HpaII CCGG 7 cut(s) 431, 450, 462, 820, 844, 1036, 1040
HphI GGTGA 3 cut(s) 164, 502, 601
Hpy166II GTNNAC 4 cut(s) 215, 874, 1078, 1174
Hpy188I TCNGA 5 cut(s) 192, 326, 805, 1151, 1224
Hpy188III TCNNGA 7 cut(s) 90, 128, 158, 536, 544, 697, 1102
Hpy8I GTNNAC 4 cut(s) 215, 874, 1078, 1174
HpyAV CCTTC 5 cut(s) 672, 771, 1108, 1258, 1321
HpyCH4III ACNGT 2 cut(s) 206, 408
HpyCH4IV ACGT 2 cut(s) 139, 734
HpyCH4V TGCA 8 cut(s) 107, 179, 302, 728, 742, 854, 955, 1013
HpyF10VI GCNNNNNNNGC 3 cut(s) 249, 399, 1091
HpyF3I CTNAG 4 cut(s) 804, 1121, 1242, 1332
HpySE526I ACGT 2 cut(s) 139, 734
Hsp92II CATG 9 cut(s) 161, 479, 634, 692, 700, 854, 959, 989, 1007
HspAI GCGC 1 cut(s) 1008
KspAI GTTAAC 1 cut(s) 874
Kzo9I GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
LmnI GCTCC 4 cut(s) 433, 568, 759, 1267
Lsp1109I GCAGC 2 cut(s) 119, 997
LweI GCATC 1 cut(s) 631
MabI ACCWGGT 1 cut(s) 876
MaeI CTAG 3 cut(s) 437, 674, 758
MaeII ACGT 2 cut(s) 139, 734
MaeIII GTNAC 1 cut(s) 969
MalI GATC 9 cut(s) 194, 236, 258, 368, 534, 548, 869, 1148, 1209
MbiI CCGCTC 1 cut(s) 502
MboI GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
MboII GAAGA 7 cut(s) 19, 25, 28, 268, 359, 517, 1211
MflI RGATCY 2 cut(s) 256, 1207
MhlI GDGCHC 2 cut(s) 756, 936
MluCI AATT 5 cut(s) 153, 341, 792, 976, 1337
MlyI GAGTC 3 cut(s) 118, 281, 968
MmeI TCCRAC 4 cut(s) 284, 349, 442, 1107
MseI TTAA 4 cut(s) 83, 873, 975, 1323
MslI CAYNNNNRTG 2 cut(s) 72, 747
MspI CCGG 7 cut(s) 431, 450, 462, 820, 844, 1036, 1040
MspR9I CCNGG 4 cut(s) 308, 820, 878, 1041
Mva1269I GAATGC 1 cut(s) 1096
MvaI CCWGG 2 cut(s) 308, 878
MwoI GCNNNNNNNGC 3 cut(s) 249, 399, 1091
NciI CCSGG 2 cut(s) 820, 1041
NcoI CCATGG 1 cut(s) 630
NdeII GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
NlaIII CATG 9 cut(s) 161, 479, 634, 692, 700, 854, 959, 989, 1007
NlaIV GGNNCC 4 cut(s) 258, 755, 782, 1065
NspV TTCGAA 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 126
PagI TCATGA 2 cut(s) 157, 696
PceI AGGCCT 1 cut(s) 678
PctI GAATGC 1 cut(s) 1096
PfeI GAWTC 2 cut(s) 321, 847
PkrI GCNGC 4 cut(s) 109, 448, 1012, 1087
PleI GAGTC 3 cut(s) 118, 281, 967
PmaCI CACGTG 1 cut(s) 140
PmlI CACGTG 1 cut(s) 140
PpsI GAGTC 3 cut(s) 118, 281, 967
Ppu21I YACGTR 1 cut(s) 140
PpuMI RGGWCCY 2 cut(s) 373, 780
PsiI TTATAA 1 cut(s) 582
Psp1406I AACGTT 1 cut(s) 734
Psp5II RGGWCCY 2 cut(s) 373, 780
Psp6I CCWGG 2 cut(s) 306, 876
PspCI CACGTG 1 cut(s) 140
PspGI CCWGG 2 cut(s) 306, 876
PspN4I GGNNCC 4 cut(s) 258, 755, 782, 1065
PspPI GGNCC 6 cut(s) 242, 361, 373, 392, 433, 780
PspPPI RGGWCCY 2 cut(s) 373, 780
PsuI RGATCY 2 cut(s) 256, 1207
RsaI GTAC 4 cut(s) 208, 1002, 1056, 1142
RsaNI GTAC 4 cut(s) 207, 1001, 1055, 1141
RseI CAYNNNNRTG 2 cut(s) 72, 747
SaqAI TTAA 4 cut(s) 83, 873, 975, 1323
SatI GCNGC 4 cut(s) 108, 447, 1011, 1086
Sau3AI GATC 9 cut(s) 192, 234, 256, 366, 532, 546, 867, 1146, 1207
Sau96I GGNCC 6 cut(s) 242, 361, 373, 392, 433, 780
SchI GAGTC 3 cut(s) 118, 281, 968
ScrFI CCNGG 4 cut(s) 308, 820, 878, 1041
SduI GDGCHC 2 cut(s) 756, 936
SexAI ACCWGGT 1 cut(s) 876
SfaNI GCATC 1 cut(s) 631
Sfr274I CTCGAG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 345
SgrAI CRCCGGYG 1 cut(s) 449
SinI GGWCC 2 cut(s) 373, 780
SlaI CTCGAG 1 cut(s) 126
SmiMI CAYNNNNRTG 2 cut(s) 72, 747
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
Sse9I AATT 5 cut(s) 153, 341, 792, 976, 1337
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 4 cut(s) 447, 500, 513, 1085
SspMI CTAG 3 cut(s) 437, 674, 758
StuI AGGCCT 1 cut(s) 678
StyD4I CCNGG 4 cut(s) 306, 818, 876, 1039
StyI CCWWGG 2 cut(s) 630, 936
TaaI ACNGT 2 cut(s) 206, 408
TaiI ACGT 2 cut(s) 142, 737
TaqI TCGA 4 cut(s) 127, 233, 337, 345
TasI AATT 5 cut(s) 153, 341, 792, 976, 1337
TatI WGTACW 1 cut(s) 1140
TauI GCSGC 2 cut(s) 449, 1088
TfiI GAWTC 2 cut(s) 321, 847
Tru1I TTAA 4 cut(s) 83, 873, 975, 1323
Tru9I TTAA 4 cut(s) 83, 873, 975, 1323
TscAI CASTG 2 cut(s) 79, 1053
TseI GCWGC 2 cut(s) 107, 1010
TspDTI ATGAA 5 cut(s) 26, 146, 174, 343, 685
TspRI CASTG 2 cut(s) 79, 1053
VpaK11BI GGWCC 2 cut(s) 373, 780
XapI RAATTY 4 cut(s) 153, 341, 792, 1337
XcmI CCANNNNNNNNNTGG 1 cut(s) 992
XhoI CTCGAG 1 cut(s) 126
XspI CTAG 3 cut(s) 437, 674, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.